Objective To analyze the stability of chromosome variant ratio of three available transformed corneal cell lines. Methods Chromosome specimens of transformed cells including human corneal epithelial cells(HCE),bovine corneal endothelial cells(BCE) and rabbit corneal epithelial cells(RCE) were prepared by a direct method using regular Giemsa staining. Chromosomes of cells in metaphase were counted under the microscope. Then, the variant ratio of chromosomes and their nuclear types were analyzed. Results The chromosome numbers were 56 to 65, 27 to 34 and 74 to 88 for HCE, BCE and RCE, respectively. Chromosome numbers in the three commonly used and transformed corneal cell lines were changed in comparison to their parent tissues. Conclusion Genotyping study may provide important information for using HCE、BCE、RCE in functional studies.

Objective To investiagte the serum adiponectin concentration in preeclampsia and its relationship with serum leptin and soluble leptin receptor levels.Method The level of adiponectin,leptin and soluble leptin receptor in serum were measured by enzyme-linked immunosorbent assay(ELISA)in 38 patients with preeclampsia and 42 patients as control.The relationship of free leptin index(leptin/soluble leptin receptor) to preeclampsia was analyzed.Results There were no significant differences in maternal age,gestational age and body mass index(BMI)between two groups.But the gestational age and birth weight were significantly lower in preeclampsia than in control.The patients with preeclampsia had significantly higher levels of serum adiponectin, leptin and free leptin index(1691.7?g/ml,37.5 ng/ml and 0.95 respectively)than the control(689.4?g/ml, 19.3 ag/ml and 0.49,respectively).But there was no significant difference in serum level of soluble leptin receptor between the groups(35.0 ng/ml vs 42.2 ng/ml).Serum adiponectin was not significantly correlated with the level of leptin,soluble leptin receptor and free leptin index.Area of serum adiponectin,leptin and free leptin index in preeclampsia under the ROC curve were less than 0.5.Conclusions The patients with preeclampsia have paradoxical higher serum levels of adiponecfin and more bioavailability of leptin,suggesting these may be important factom of this complication.

Child ; Epiphyses ; Fracture Fixation, Internal ; Humans ; Lower Extremity

Objective To compare the phonetic parameters changes of titanium denture base materials to that of conventionally processed poly methyl methacrylate(PMMA)resin.Methods Forty-two edentulous patients were included in this study.Each patient received two sets of dentures.One of the upper denture was restored with titanium denture base,the other was restored witll conventional PMMA denture base.Consonant /x,sh,r,zh,ch,j,q/associated with place of articulation and manner of articulation were chosen and all of the processed acoustic specimens were stored at computerized speech lab(CSL).Model 4150 (KAY) for the first concentrated frequency area (CFA-1),voice onset time (VOT) and spectrogram were analyzed.Results The consonant articulation effect of denture base showed significant difference between the two type of dentures in the CSL.The consonant parameters(/x,sh,r,zh,ch,j,q/)CFA-1 of group A [(3595±102),(3089±104),(1876±116),(4078±116),(3604±119),(5432±118),(4934±121)Hz]was different from the one of group B[(3644±101),(3280±88),(1978±113),(4214±193),(3817±49),(5478±158),(5020±183)Hz](P<0.05).The consonant parameters VOT of/zh,ch,j/in group A[(54.67±1.13),(143.80±2.24),(62.53±0.75)ms]was different from those of group B[(52.88±0.34),(137.55±2.50),(62.00±1.54) ms](P<0.05).The consonant parameters VOT of/g,k/in group A [(26.94±0.33),(114.53±2.15)ms] was different from those of group B [(28.59 4-0.65),(124.40±3.74) ms](P<0.05).A redundant spike as it was located prior to the inherent spike could be observed in the/g,k/spectrogram of the PMMA denture base group.Conclusions Titanium denture base is recommended to provide a satisfying form of articulate position with thinner front Palate thickness of the denture base.CFA-1 and VOT based CSL are effective acoustic parameters for articulation testing after denture delivery.

Objective To review the techniques used in biliary reconstruction for adult-adult living donor liver transplantation using a right lobe graft. Methods The clinical data of 21 pairs of donor and recipient who underwent right lobe living donor liver transplantation from April 2007 to May 2009 at Beijing Youan Hospital were analyzed retrospectively. Biliary anastomoses consisted of 10 single right hepatic duct to common hepatic duct anastomoses, 5 donor double branched ducts to recipient double branched ducts anastomoses, 5 single anastomoses between a donor double branched duct which had been converted to a single duct by ductoplasty to a single recipient bile duct, and 1 hepaticojejunostomy. A T-tube was inserted through the anterior wall of the common hepatic duct and splinted across the anastomosis in 2 recipients and a Y-tube was used in 1 recipient. Results 4 recipients died during the first post-transplant month. Another recipient received a retransplantation for acute liver necrosis. The remaining recipients were alive. The 1-year survival rate of the recipients was 77.65 %.5 patients developed biliary leakage and 2 patients developed biliary stricture. The 7 biliary complications were treated and cured by further surgical procedures. There was no significant difference in the biliary complications among the three different types of biliary anastomotic groups (x2 = 0. 659,P=0. 719). Conclusion The different types of biliary anastomoses can be used in living donor liver transplantation depending on the situations found in the donors and recipients. Continuous suturing on the posterior wall of the bile duct, interrupted suturing on the anterior wall and microsurgical techniques in biliary reconstruction are effective modalities to minimize biliary complications.

OBJECTIVETo analyze the variation of serum insulin levels in critically ill children and investigate the underlying mechanism and clinical significance to provide the basis for treatment.

METHODTotally 332 critically ill children admitted in pediatric intensive care unit (PICU) of Hunan Children's Hospital from Nov., 2011 to April, 2012 were studied. The high insulin group (n = 332) was defined as insulin levels within 24 h > 11.1 mU/L and was divided into 2 groups: mildly elevated group (n = 194): 11.10 - 33.30 mU/L, increased three times group (n = 138): > 33.3 mU/L. Insulin, C-peptide and blood glucose were measured within 24 hours after admission, on day 3 and 7. Other results of inflammatory markers, lactate, cardiac enzymes, amylase, pancreatic ultrasound, hepatic and renal function as well as indicators related to severity and prognosis were recorded after admission.

RESULTThe peak of insulin level was seen on day 1, then presented a downward trend and reached the normal level on day 7. The peaks of blood glucose and C-peptide level were seen on day 1 then declined, the levels on day 7 were still slightly higher than normal level. The insulin level on admission (41.47 ± 30.85) mU/L were positively correlated with lactic acid (2.29 ± 1.81) mmol/L and procalcitonin level (5.08 ± 6.70) ng/ml (r = 0.370, P = 0.000; r = 0.168, P = 0.002) (P < 0.01). The insulin level on admission in children with 1 organ failure (41.24 ± 22.60) mU/L or 2 or multiple organ failure (48.98 ± 22.17) mU/L was higher than that in children with non-organ failure (34.11 ± 29.84) mU/L (U = 1621.001, P = 0.000;U = 1300.000, P = 0.000) (P < 0.01). The insulin level on admission in death group (52.99 ± 32.34) mU/L was higher than that in survival group (32.85 ± 24.10) mU/L (U = 1585.000, P = 0.000) (P < 0.01). Ten cases in death group were complicated with pancreatic damage and the average insulin level on admission was (65.29 ± 50.53) mU/L.

CONCLUSIONThe high insulin level was correlated with the degree of inflammatory response, ischemia and hypoxia. The high insulin level in critically ill children was relevant to the pancreatic damage, the severity of the disease, organ dysfunction, and evaluation of prognosis.

Adolescent ; Blood Glucose ; metabolism ; C-Peptide ; blood ; Calcitonin ; blood ; Calcitonin Gene-Related Peptide ; Child ; Child, Preschool ; Critical Illness ; Female ; Humans ; Infant ; Infant, Newborn ; Insulin ; blood ; Intensive Care Units, Pediatric ; Male ; Multiple Organ Failure ; blood ; mortality ; Pancreas ; metabolism ; pathology ; Prognosis ; Protein Precursors ; blood ; Survival

Objective To observe the clinical efficacy and safety of voriconazole tablets combined with caspofungin injection in the treatment of hematological disease with invasive pulmonary aspergillosis.Methods A total of 100 patients of hematologic disease with invasive pulmonary aspergillosis were randomly divided into control group and treatment group with 50 cases per group.Control group was given amphotericin B,the first dose was 5 mg,the maximum dose of 25 mg at a speed of 5 mg · d-1,and then the dose of 25 mg · d-1 for 10 days,qd,intravenous drip,which was treated for 15 d.Treatment group was given caspofungin,the first dose of 70 mg,adjusted to 50 mg,qd,intravenous drip + voriconazole,the first day of 6 mg · kg-1,the second day adjusted to 4 mg · kg-1,bid,orally,which was treated for 15-28 d (treatment to full effect).The clinical efficacy and adverse drug reactions were compared between two groups.Results After treatment,the main indexes in treatment and control groups were compared:the total effective rates were 88.00％ (44/50 cases) and 68.00％ (34/50 cases),the total effective rates with empirical treatment were 92.31％ (24/26 cases) and 81.48 ％ (22/27 cases),the total effective rates with proactive treatment were 83.33％ (20/24 cases) and 52.17％ (12/23 cases),the differences were statistically significant (all P ＜ 0.05).The adverse drug reactions in treatment group were based on nervous system damage and liver and kidney dysfunction,which in control group were based on nervous system damage,peripheral edema,liver and kidney dysfunction and visual abnormalities.The incidences of adverse drug reactions in treatment and control groups were 4.00％ and 14.00％ with significant difference (P ＜ 0.05).Conclusion Voriconazole tablet combined with caspofungin injection has definitive clinical efficacy and safety in the treatment of hematological disease with invasive pulmonary aspergillosiss.

Adult ; Aortic Coarctation ; therapy ; Catheterization ; Humans ; Male ; Platinum ; Stents

OBJECTIVETo find a new and effective way for the transfection of adenovirus vectors encoding HSP70 cDNA, so as to provide another possible method in gene therapy against ischemia and cellular hypoxia after burn injury.

METHODSThe replicated defective adenovirus vectors encoding HSP70 cDNA were encapsulated. Its acid resistance and dissolution in intestinal fluid were tested in artificial gastric juice and intestinal fluid. The expression of HSP70 gene which was transfected by the microcapsules orally was detected by RT-PCR.

RESULTSThe encapsulated replicated defective adenovirus vectors were viable in vitro. They exhibited good resistant to acid (resolution ratio less than 10%) and dissolution in intestinal juice (resolution ratio higher than 50%). The HSP70 gene expression of the tested rats was significantly higher than control, but there was no difference in the quantity of HSP70 induced by sodium arsenite or adenovirus transfection through injection by vein.

CONCLUSIONThe encapsulation of adenovirus vectors can successfully keep the viability of the virus in vitro and protect the virus from harmful effect of acid and enzyme in the gastric juice. Its nice dissolution in intestinal juice should ensure its absorption by oral transfection. The expression of the HSP70 gene after oral intake of this preparation is as high as that with other traditional transfection methods. It is possible that in the future the encapsulated replication of defective adenovirus vectors encoding HSP70 cDNA can provide a safer, convenient and effective way for gene therapy for burn patients.

Adenoviridae ; genetics ; Animals ; Burns ; complications ; Capsules ; Genetic Therapy ; Genetic Vectors ; HSP70 Heat-Shock Proteins ; administration & dosage ; genetics ; Hypoxia ; etiology ; prevention & control ; Intestinal Mucosa ; metabolism ; Ischemia ; etiology ; prevention & control ; Male ; Myocardium ; metabolism ; Rats ; Rats, Wistar ; Transfection

OBJECTIVETo give some theory support of Cistanche tubulosa cultivation by searching dry matter accumulation and echinacoside content of C. tubulosa.

METHODDry matter accumulation content of C. tubulosa culturing in Huabei plain was analysed in different growth season of C. tubulosa. Echinacoside content was determined by HPLC.

RESULTDry matter accumulation of C. tubulosa showed "S" variation. Dry matter accumulation increased fastest in September among growing seasons. Dry matter amount was 138.58 g after C. tubulosa grew a year. Dry matter amount decreased significantly along with inoculation time retarded. Echinacoside content was 30.59% when C. tubulosa grew in 5 months, decreased guadully after that, and 9.76% in annual.

CONCLUSIONVariation rule of dry matter accumulation and echinacoside content was found in C. tubulosa that grew one year in Huabei plain.

Biomass ; China ; Cistanche ; chemistry ; growth & development ; Glycosides ; metabolism ; Plants, Medicinal ; chemistry ; growth & development ; Seasons