1.Analysis of paraplegia during transarterial chemoembolization in liver cancer
Bo ZHOU ; Zhi-Ping YAN ; An-Le WU ;
Journal of Interventional Radiology 2006;0(12):-
Objective To evaluate the pathophysiology of paraplegia during transarterial chemoembolization in liver cancer and investigate effective management and prevention for improving clinical situation and relieve major symptoms.Method 2758 patients accepted TACE procedure because of liver cancer(and/or combined with remote metastasis),demonstrated 4 cases suffering from paraplegia(3 males,1 female)since Mar.2003 to Feb.2005,with mean age of(51?14)years old,The operative records and the clinical features after chemoembolization were summarized in detail.Results The incidence of paraplegia was 0.145%,with major symptoms of dysesthesia and hypokinesise emerging within 4 hours after TACE,and most symptoms aggravating gradually within 24-48 hours untill paraplegia appeared,and then turned to stabilization about 5-7 days,with their body functions partial recovery in 2 months.Conclusion More attention should be paied to prevent ectopic embolization of spinal cord vessels originating from extrahepatic collateral arteries during TACE or TAE.
2.Primary mucosal melanoma of the nasal cavity: a linicopathological analysis of 17 cases
Wen LIU ; Zhi ZENG ; Heng ZHOU ; Hao WU ; Jingping YUAN
Chinese Journal of Clinical and Experimental Pathology 2017;33(2):169-173
Purpose To study the clinicopathologic features and differential diagnosis of primary mucosal melanoma of the nasal cavity (PMMNC).Methods 17 cases of PMMNC diagnosed from January 2003 to September 2016 were studied by clinical pathological analysis and immunohistochemical staining,and relevant literatures were reviewed.Results 73% of the PMMNC was characterized by unilateral nasal congestion and intermittent epistaxis and 61% of the PMMNC occurred in the nasal septum and nasal side wall.Microscopically,the organizational structure and morphology were complex and diverse,which had several cell types including epithelioid cell type (6cases,35.3%),spindle cell type (3 cases,17.6%) and snall cell type (5 cases,29.4%),the other 3 cases (17.6%)were mixed cell type.Mitotic activity and tumor necrosis were more likely to be seen in PMMNC,among other clinicopathological features with a small amount of fibrous stroma and melanoma and rich blood vessels.The immunohistochemical study showed that the positive rate of S-100 and HMB-45 were both 93.8%(15 cases) and those of Melan-A and vimentin were both 87.5% (14 cases),while CK and EMA were both negative (16 cases).Conclusion PMMNC is a rare disease and the phenotype of S-100,HMB-45,Melan-A and vimentin are useful for diagnosis of PMMNC.
3.The impact of Pan3CSK4 on the platelet activation and the expression of TLR2 on it
Benquan WU ; Mingjun ZHI ; Hui LIU ; Yuqi ZHOU ; Tiantuo ZHANG
Chinese Journal of Microbiology and Immunology 2010;30(6):516-520
Objective To investgate role of TLR2 in the activation, the innate immune and inflammation of human platelets. Methods Human washed platelets were separated from healthy people(n=5) and were stimulated with different concentrations(1μg/ml, 5μg/ml, 10μg/ml) of TLR2 agonistPam3CSK4(a synthetic bacterial lipoproteins). Then the platelet aggregation rate, the expression of CD62p and TLR2 on the platelet surface were measured. Results The platelet aggregation rate were (28.32±5.67)%, (52.56±8.54)% and (76.24±11.23)%, respectively, at concentration of 1μg/ml, 5μg/mland 10μg/ml of Pam3CSK4, more than (12.83±2.43)% at 0μg/ml of it. In addition, the expression of CD62p were (18.45±2.66)%, (22.45±2.04)%, (29.53±4.08)%, respectively at above concentration of Pam3 CSK4, more than (11.20±1.67)% of CD62p at control group(P<0.01). The expression of TLR2 was not significantly increased at a lower concentration of Pam3CSK4(1μg/ml) with (16.85±6.10)% compared with(10.81±3.99)% at the control group. However, it were (21.15±9.90)% and (22.52±9.26)%, respectively, at a higher concentration(5μg/ml, 10μg/ml)more than(10.81±3.99)% at the control group(P<0.05). Conclusion Pam3CSK4 induce aggregation, activation and the up-regulation of TLR2 of platelet by stimulating TLR2 receptor of it. Thereafter, TLR2 play an important role in the innate immune of platelet.
4.Observation on effect of qi-supplementing blood-activating and stasis-removing principle in treating patients with systemic lupus erythematosus of blood-stasis type.
Jia-li WU ; Xio-hui BAO ; Zhi-lin ZHOU
Chinese Journal of Integrated Traditional and Western Medicine 2003;23(11):860-861
Adolescent
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Adult
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Anti-Inflammatory Agents
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therapeutic use
;
Diagnosis, Differential
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Dipyridamole
;
therapeutic use
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Drug Therapy, Combination
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Drugs, Chinese Herbal
;
therapeutic use
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Female
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Humans
;
Lupus Erythematosus, Systemic
;
drug therapy
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Male
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Medicine, Chinese Traditional
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Middle Aged
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Phytotherapy
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Prednisone
;
therapeutic use
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Tripterygium
;
chemistry
5.Updates on study of glioma stem cells.
Zhi-hua ZHOU ; Liang YI ; Xiu-wu BIAN
Chinese Journal of Pathology 2007;36(3):201-203
AC133 Antigen
;
Animals
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Antigens, CD
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metabolism
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Cell Differentiation
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Cell Proliferation
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Drug Resistance, Neoplasm
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Glioma
;
pathology
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Glycoproteins
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metabolism
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Humans
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Neoplastic Stem Cells
;
metabolism
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pathology
;
physiology
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Neovascularization, Pathologic
;
etiology
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pathology
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Peptides
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metabolism
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Radiation Tolerance
6.Detective Significance of Cerebral Electrical Admittance Plethysmogram in Newborns with Asphyxia
ke-ying, ZHOU ; jin-zhi, SONG ; jing-zhi, LI ; hui, LUO ; xue-chang, XIAO ; zhi-guang, LI ; ben-qing, WU
Journal of Applied Clinical Pediatrics 2006;0(20):-
Objective To study hemodynamics of cerebral blood flow in newborns with asphyxia.Method Bilateral cerebral electrical admittance plethysmogram(BCEAP) was used to explore characteristics of cerebral blood flow in 20 healthy newborns and 20 newborns with asphyxia respectively from first to fourth day after birth.Results Both the ratio of Hs to b-S(Hs/ b-S) and index of admittance differential loop(ADL) Ⅰ+Ⅱ decreased significantly in newborns with asphyxia compared to normal control from first to fourth day after birth(P
7.The effect of combined application of low dose fentanyl and midazolam on sodium channels in rat cerebral cortical neurons.
Yun-Chun YANG ; Xian ZHOU ; Jia-Li WU ; Xuan JIANG ; Shu-Zhi ZHOU ; Xiao-Bin WANG
Chinese Journal of Applied Physiology 2011;27(1):85-87
Anesthetics, Intravenous
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administration & dosage
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pharmacology
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Animals
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Animals, Newborn
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Cerebral Cortex
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cytology
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metabolism
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Drug Synergism
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Female
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Fentanyl
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administration & dosage
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pharmacology
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Male
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Midazolam
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administration & dosage
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pharmacology
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Neurons
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metabolism
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Patch-Clamp Techniques
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Primary Cell Culture
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Rats
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Rats, Sprague-Dawley
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Voltage-Gated Sodium Channels
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drug effects
9.Effect of chlorine gas pulmonary emphysema on IL-8 and TNF in rats.
Shan WU ; Zhi-xie JUNG ; yan-lung SHEN ; Ming SHEN ; Yi ZHOU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2003;21(4):283-284
Animals
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Chlorine
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toxicity
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Interleukin-8
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metabolism
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Lung
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drug effects
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metabolism
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pathology
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Male
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Pulmonary Emphysema
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chemically induced
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metabolism
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Rats
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Rats, Wistar
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Time Factors
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Tumor Necrosis Factor-alpha
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metabolism
10.UDP-Ga1NAc: polypeptide α-N-acetygalactosaminyltransferase 2 Localized on Both cis and trans Side of Golgi Stacks in SGC7901 Cells
Yinghui ZHOU ; Saiyu HANG ; Hao QIU ; Wei JIA ; Lan XU ; Zhi JIANG ; Shiliang WU
Progress in Biochemistry and Biophysics 2009;36(1):49-57
Uridine diphosphate (UDP)-GalNAc : polypeptide N-acetylgalactosaminyltransfemse (ppGalNAcT) catalyzes the initial step in mucin type O-glycosylation in the Golgi apparatus. Here generation and characterization of a polyclonal antibody to human ppGalNAcT2 were described. The subcellular location of ppGalNAeT2 in SGC7901 cell line was investigated using Western blot analysis of fractionated cell extracts and confocal microscopy with this antibody and two Golgi markers: Golgi SNARE (soluble N-ethylmalemide-sensifive factor attachment protein receptor) of 28 ku (GS28) and trans-Golgi network (TGN) 38, markers for the c/s- and trans-Golgi apparatus, respectively. Morphometric analyses indicated that ~60% of the ppGalNAcT2 signal colocalized with the GS28, while~36% of the c/s-Golgi marker colocalized with the ppGalNAeT2. Approximately 34% of the ppGalNAcT2 signal colocalized with the TGN38, whereas 38% of the trans-Golgi marker colocalized with the ppGalNAcT2. The results provide unequivocal evidence for the location ofppGalNAcT2 within the Golgi apparatus, and further highlight the importance of this organelle in the initiation of O-linked glycosylation.