2.Domestic shock wave lithotripters and their characteristics
Chinese Medical Equipment Journal 2004;0(08):-
Although there is great technological disparity between the best foreign lithotripters and domestic ones, the clinical efficacy of the latter is quite good. In this article, the characteristics, developing status and clinical applications of domestic lithotripters are introduced.
4.Value of three-dimensional endometrial volume and 3-dimensional power Doppler imaging in predicting endometrial polyps and endometrial hyperplasia
Wei ZHAO ; Zhi LIU ; Cai CHANG
Chinese Journal of Ultrasonography 2010;19(1):40-42
Objective To evaluate the value of endometrial volume measurement and 3-dimensional power Doppler imaging(3D-PDI) in the differentiation between endometrial polyps and endometrial hyperplasia.Methods One hundred and fifty-six women who diagnosed as thick and inhomogeneous endometrium or hyperechogenic focal intrauterine structures by 2D ultrasound were enrolled,including 50 patients with endometrial polyps in the proliferative phase,51 cases with hyperplasia and 55 cases with normal histology or others.All were scheduled for hysteroscopy,dilatation and curettage,and the ultrasound was performed within 24 hours before the procedure. Endometrial volume, vascularity index (VI), flow index (FI) and vascularity flow index (VFI) were measured. These parameters were compared between endometrial polyps group and endometrial hyperplasia group.Results These parameters (endometrial volume,VI,FI and VFI) were all considered statistically significant between two groups.Conclusions Endometrial volume and 3D-PDI are good diagnostic tools in the differentiation between endometrial polyps and endometrial hyperplasia.
5.Expression of lysozyme of macrophages of alveolus in rats exposed to quartz.
Wei-wei SUN ; Zhi-fang SONG ; Zhao-yu ZENG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2006;24(9):558-559
Animals
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Macrophages, Alveolar
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drug effects
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enzymology
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Male
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Muramidase
;
biosynthesis
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Quartz
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toxicity
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Rats
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Rats, Sprague-Dawley
6.Chronic mucor invasive fungal sinusitis with intraorbital extension: a case report.
Zhao-wei GU ; Zhi-wei CAO ; Xin-jia ZHOU
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2009;44(1):76-77
Aged, 80 and over
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Chronic Disease
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Female
;
Humans
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Mucor
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pathogenicity
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Mucormycosis
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microbiology
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pathology
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Orbit
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pathology
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Sinusitis
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microbiology
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pathology
7.Analysis of influencing factors on adverse reaction of Shengfu injection based on prospective active safety monitoring.
Zhi-fei WANG ; Wei ZHAO ; Yin ZHANG ; Yan-ming XIE
China Journal of Chinese Materia Medica 2015;40(24):4746-4751
This study collected demographic data, past history, personal history, family history, dosage, solvent, combined medication information of adverse reaction cases from a prospective, multi center, large sample intensive hospital monitoring, and found the influencing factors with cross-tab analysis. The results showed that in the medication group of 19-45, patients with allergic histories had a higher proportion in ADR; in the medication group of 46-65, male patients with allergic histories had a higher proportion in ADR; indication and non-indication group, patients of 19-45 years old, with combined medications and allergy histories had a higher proportion in ADR; Non-indication medication group, patients with combined medication, higher concentration, out-instruction solvent and dosage, had a higher proportion in ADR. So, the ADRs of Shenfu injection were related to the history of drug allergy, and also related to the indication, dosage, solvent, concentration when it was used.
Adult
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Aged
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Drug Hypersensitivity
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etiology
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Drugs, Chinese Herbal
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adverse effects
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Female
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Humans
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Injections
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Male
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Middle Aged
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Prospective Studies
8.Experimental study of the oriented differentiation of bone marrow derived mesenchymal stem cells into chondrogenic phenotype in a specific culture fluid
Xiaofei DING ; Jingmin ZHAO ; Weiping CHEN ; Zhi YANG ; Wei SU
Chinese Journal of Tissue Engineering Research 2005;9(34):167-169
BACKGROUND:To construct tissue engineering cartilage would open up a novel way for the repair of cartilage damage in avoidance of the disadvantages of traditional therapeutic method.OBJECTIVE: To probe the techniques for the isolation of mesenchymal stem cells (MSCs) from bone marrow, as well as the in vitro differentiation into chondrocytic phenotype in a specific culture fluid.DESIGN:A complete randomized experimentSETTING:The Department of Traumatic Orthopedics and Hand Surgery of the First Affiliated Hospital of Guangxi Medical University, and Teaching and Research Faculty of Histology and Embryology of Guangxi Medical University.METHODS: The experiment was carried out at Guangxi Medical University between August 2002 and April 2003. Twenty SD neonatal weaning rats were selected. Bone marrow was aspirated from the bones of rat limbs and was isolated by gradient centrifugation in Percoll, and MSCs could be obtained in combination with adherent screening method, which were then cultured in DMEM-LG with 15% fatal bovine serum (FBS) in the incubator of 37℃ with 5% CO2 for 10-14 days. The passage cells were induced in DMEM-HG with 15% FBS (containing TGF-β1 10 μg/L, 10-7 mol/L dexamethasone, 50 mg/L VitC).MAIN OUTCOME MEASURES :The morphology, growth, as well as proliferation and specific expression of chondrogenic matrix of in vitro cultured MSCs due to specific induction.RESULTS: Totally 20 SD rats were observed and analyzed with no loss SCs grew in visible symmetric colonies, displaying a long-spindle shape,and the morphological characteristics of marrow-derived MSCs had no obvious changes during passage-culture, but its proliferation time was found from a shuttle fibroblastic appearance to polygonal shape, displaying posiHC staining of type Ⅱ collagen of cartilage specific matrix.bronectin adherent screening technique is a convenient, effective and practical method to separate and collect MSCs from rat bone marrows in chondrogenic phenotype when induced by a specific medium and can secrete cartilage specific matrix, and they can be the optimal seed cells for cartilage tissue engineering.
9.Effects of MCTP on production of nitric oxide and expression of eNOS protein of cultured pulmonary artery endothelial cells and contractility of cultured pulmonary artery smooth muscle cells
Wei CHENG ; Honglin MA ; Peiqi WANG ; Wenming ZHAO ; Zhi LI
Chinese Pharmacological Bulletin 2003;0(09):-
Aim To investigate the effects of monocrotaline pyrrole on production of nitric oxide and expression of eNOS protein of cultured pulmonary artery endothelial cells and on contractility of cultured pulmonary artery smooth muscle cells.Methods DAF-2 fluorescence technique was used to determine NO level,Western blot analysis was performed to determine the level of eNOS protein,and collagen gel contraction system was adopted to analyze muscle contractility.Results NO production induced by ACh and expression of eNOS protein were obviously inhibited by monocrotaline pyrrole compared with those of control group and gel contraction area in MCTP-treated cells induced by Thapsigargin obviously decreased.Conclusions monocrotaline pyrrole could inhibit the level of the ACh-induced production of NO and expression of eNOS protein,and enhance the contractility of pulmonary artery smooth muscle cells,which may be one of the possible mechanisms of MCTP-induced pulmonary artery hypertension.
10.Simultaneous Determination of Four Major Steroidal Saponins in seven species of Dioscorea L.By HPLC-ELSD
Zhi SHEN ; Wentinge ZHANG ; Weiliang ZHAO ; Wei HUANG
Chinese Herbal Medicines 2011;03(1):70-74
Objective To control the quality of the species in Dioscorea L. better. Methods An HPLC-ELSD method was developed for the first time to simultaneously determine four bioactive ingredients:dioscin gracillin,protoneodioscin, and protoneogracillin in 31 samples belonging to seven species of Dioscorea L. from different areas. The column was an Inertsil HILIC (250mmx4.6 mm,5pm). The separation was carried out with a gradient program. The mobile phase was acetonitrile-water at a flow rate of 0.8 mL/min. Results The standard curve was rectilinear in the range of 0.464-12.97 gg (r=0.9969) for dioscin, 0.310-7.09 ltg (r = 0.9953) for gracillin, 0.469-11.66 gg (r=0.9970) for protoneodioscin, and 0.276-6.87 gg (r=0.9992) for protoneogracillin. The recoveries of the markers were 98.1%, 100.1%, 97.2%, and 96.4%, respectively. The contents of the four components were quite different among the seven species of Dioscorea L. Conclusion The proposed HPLC-ELSD method is convenient,fast, accurate, and applicable for simultaneous analysis of multiple bioactive components of species in Dioscorea L.for quality control, which could facilitate discovering new natural resources of steroidal saponin.