Aim To investigate the changes of [Ca2+]i in the pulmonary artery smooth muscle cells of PAH rats induced by MCT.Methods PAH rat model was established by MCT intraperitoneal injection.The PASMCs were primarily cultured and loaded with Fura-2/AM.Effects of Ryanodine receptor agonists on intra-cellular calcium were measured by Fluorescence microscopy Results After being given 10 nmol?L-1 Ryanodine,the concentration of [Ca2+]i in control group increased by(93.31?12.41)nmol?L-1;and the concentration in PAH group increased by(141.71?13.59)nmol?L-1(P

Defecation ; Humans ; Male ; Middle Aged ; Postoperative Complications ; physiopathology ; therapy ; Spinal Neoplasms ; surgery ; Urination

Objective To analyze the relation between the CT changes of ventricular volume and prognosis in newborn of perinatal hypoxic-ischemic brain damage.Methods From Jan. 2002 to Dec. 2006, 105 newborns with perinatal hypoxic-ischemic brain damage (moderate to severe) were divided into 2 groups (group A with nervous system sequel and group B without nervous system sequal). Brain CT was performed in 24 hours after admission in all cases and at 1 and 3-month time point in some cases according to their convalescence condition. Huckman index,the third ventricular width on CT images of various time points were measured and calculated by radiologist. The relations between the values and prognosis were analyzed statistically.Results The huckman index increased gradually with rehabil (P

Adolescent ; Child ; Child, Preschool ; Female ; Homeodomain Proteins ; genetics ; Humans ; Infant ; Male ; Oncogene Proteins, Fusion ; genetics ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; genetics

Child ; Child, Preschool ; Humans ; Leukemia ; immunology ; prevention & control ; Vaccination

Cholesterol ; metabolism ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Metabolic Diseases ; prevention & control ; Phytotherapy

OBJECTIVE: To study the effects and mechanisms of danhong injection on cerebral ischemia reperfusion injury in rats with hyperlipidemia. METHODS: Hyperlipidemia model was established by feeding SD rats with high-fat diet for 6 weeks. Then hyperlipidemia rats were randomly divided into sham operation group, model group and danhong injection low (0.5 mL•kg-1), medium (1.0 mL•kg-1) and high (2.0 mL•kg-1) dose groups with six rats for each group. Danhong injection groups were injected for 7 consecutive days before the establishment of the model of middle cerebral artery occlusion (MCAO), and the corresponding dose was given once at 1 h before modeling and 6 h after reperfusion. After 24 h of reperfusion, rats were evaluated in terms of neurological function. The changes of histopathology were observed in the cerebral cortex of ischemic rats by HE staining; RT-PCR was used to detect the mRNA expressions of Nox2, Rac1, ASC, NLRP3, caspase-1, IL-1β and IL-18 in cerebral ischemia area. In addition, the content of triglyceride (TG) and total cholesterol (TC) in serum were detected by the kits. The expression of NLRP3 protein was evaluated by immunohistochemical. RESULTS: Compared with MCAO group, Danhong injection groups improved the reperfusion injury of cerebral ischemia in varying degrees, significantly reduced the mRNA expression of Nox2, Rac1, ASC, NLRP3, caspase-1, IL-1β and IL-18, increased the neurological score of MCAO rats, and reduced the contents of TC and TG besides the expression of NLRP3 protein. CONCLUSION: Danhong injection could effectively regulate blood lipid level and play a obvious protective effect on the reperfusion injury in MCAO rats with hyperlipidemia. The mechanism might be related to the inhibition of the formation of NLRP3 inflammatory body and the decrease of the inflammation-related factors expression of caspase-1, IL-1β and IL-18.

0.05).The volume and Hct of suspension RBC and leukocyte depleted suspension RBC made by the above two methods were(144.01?11.54) ml,(152.45?15.39) ml,(158.71?10.69) ml and 0.48?0.03,0.50?0.03,0.53?0.05,respectively,and all the differences had statistical significance(P

Objective To study the role of Foxp3 + CD4 + CD25 + regulatory T cells(Foxp3 + CD4 + CD25 + Tregs) and Foxp3mRNA in pathogenesis of passive transferred myasthenia gravis(PTMG) by detecting their expression in a PTMG model.Methods Sixteen C57BL/6 young female mice were divided into model group and control group.A PTMG model was established by injecting Ringer's solution containing 1.0 mg/kg mAb35 into abdominal cavity.Mice in control group were injected with Ringer's solution not containing mAb35.Levels of CD4 + CD25 + T cells and Foxp3 + CD4 + CD25 + Tregs in spleen cells were measured by flow cytometry.Expression of Foxp3 mRNA in spleen cells was detected by real-time fluorescent quantitative polymerase chain reaction(RT-FQ-PCR).Serum level of interleukin-2 and interferon-? was detected by ELISA.Results The ratio of CD4 + CD25 + T cells and CD4 + T cells was higher in model group(8.82 ? 0.74)% than in control group(9.89 ? 0.88)%(P

Acetates ; therapeutic use ; Child ; Child, Preschool ; Female ; Humans ; Leukotriene Antagonists ; therapeutic use ; Male ; Quinolines ; therapeutic use ; Rhinitis, Allergic, Perennial ; drug therapy ; Rhinitis, Allergic, Seasonal ; drug therapy ; Single-Blind Method