1.The changes of transforming growth factor-β1 in pulmonary fibrosis induced by paraquat in mice1
Xuesong DONG ; Shengye LIU ; Wei LIU ; Shuying LIU ; Zhi LIU
Chinese Journal of Emergency Medicine 2011;20(8):826-829
Objective To study the changes of transforming growth factor-β1(TGF-β1) in pulmonary fibrosis induced by paraquat (PQ) with immunohistochemistry method. MethodsA total of 58 C57BL/6J male mice were randomly (random number) divided into the experimental group and control group. Pulmonary fibrosis was induced by intra-peritoneal injection of PQ in dose of 10 mg/kg into the mice of experimental group (n = 48), while physiological saline was used instead in mice of control group (n = 10). The mice of experimental group were sacrificed 2, 5, 7 and 14 days after PQ poisoning and the mice of control group were sacrificed on the 7th day after saline administration. Lungs of mice were taken and histological changes in lungs were evaluated by haematoxylin-eosin stain, and TGF-β1 was determined with immunohistochemistry method. The integrated optical density (iOD) value of TGF-β1 was measured and analyzed. ResultsThe TGF-β1 was markedly increased in macrophages during the genesis of pulmonary fibrosis induced by PQ. As the course of fibrosis progressed, the positive staining of TGF-B1 was mainly seen in macrophages and neutrophil's cytoplasm. On the 14th day after PQ poisoning, TGF-β1-positive cells were also detected in the fibroblast and myo-fibroblast inside the fibroblastic foci. Compared with the control group, the iOD value of TGF-β1 increased in experimental group (P < 0. 01 ) and it gradually upgraded during the course of fibrosis. Conclusions The TGF-β1 significantly increased during the course of pulmonary fibrosis induced by PQ and played an important role in the pathogenesis of the disease.
2.Evaluation of early diagnosis and treatment of cancer.
Zhi-wei DONG ; You-lin QIAO ; Gui-qi WANG
Chinese Journal of Oncology 2012;34(8):637-640
3.A pilot study on purification of human plasminogen from cord blood plasma and inducing posterior vitreous detachment
Wei-zhi, CHEN ; Jian-qiang, DONG ; Xiao-xin, LI
Chinese Journal of Experimental Ophthalmology 2011;29(8):690-696
Background Many studies and clinical trials of pharmacologic vitreolysis are already under way to try to improve vitreo-retinal surgery and to liquefy and detach the vitreous from the retina ultimately, including chondroitinase,hyaluronidase,dispase and plasmin. However, there has not been any report on purification of human plasminogen from cord blood plasma and inducing posterior vitreous detachment of the animal eye at present.Objective This study was designed to isolate and purify the production of human plasminogen (Plg) from cord blood plasma with ethanol precipitation and evaluate the efficacy of Plg in inducing posterior vitreous detachment (PVD).Methods Human Plg was Separated and purified from cord blood plasma by ethanol precipitation method. The protein band corresponding to Plg with molecular mass of 92 000 was revealed in SDS-PAGE and confirmed by MALDI-TOF and Mascot database. Anion-exchange chromatography and plasminogen activity assay kit were used to obtain purified Plg with biological activity. Twenty-five fresh pig eyes were enucleated and assigned to 5 groups and 5 eyes for each group. The normal eyes were used as control group. Balanced salt solution(BSS)of 0.1 ml was intravitreally group and standard substance group. All of the eyes were then incubatedfor 60 minutes under the 37 ℃. Retinal histopathology and ultrastructure were examined under the light microscopy, scanning electron microscopy ( SEM ) and transmission electron microscopy (TEM). Results The Plg with potential fibrinolytic activity was successfully extracted and purified from cord blood plasma by ethanol precipitation method. No posterior vitreous detachment (PVD) was seen in normal control group, BSS group and r-SK group following the intravitreal injection under the sem. However,PVD was demonstrated in r-SK+ Plg group and standard substance group under the SEM. The inner limiting membrane ( ILM ) and the retina were well preserved in all of the experimental eyes. No retinal morphology and ultrastructural abnormality were found under the light and SEM and TEM. Conclusion Ethanol precipitation is a feasible way to isolate and purify Plg from human cord blood plasma. Extracted Plg shows potential fibrinolytic intravitreal injection of Plg.
4.Management of deep vein thrombosis of lower limbs in patients with severe craniocerebral injury
De-zhi LI ; Shao-dong ZHANG ; Wei-min SUN
Chinese Journal of Rehabilitation Theory and Practice 2004;10(7):402-403
Objective To explore the management of deep vein thrombosis (DVT) of lower limbs in patients with severe craniocerebral injury.Methods The clinical data of 9 patients of severe craniocerebral injury with DVT were analyzed respectively.Results All 9 patients were given medicine therapy including thrombolytic, anti coagulating, anti platelet aggregation and antibiotics. 3 cases were cured, 1 case was improved, 4 cases died and 1 case discharged by himself. Conclusion There are risk factors for DVT in patients with severe craniocerebral injury. Early prophylaxis is important. Early diagnosis and treatment are benefited.
5.Analysis of four flavonoids in Lysimachia clethroides using ionic liquid-assisted extraction.
Jin-feng WEI ; Zhi-juan ZHANG ; Dong-dong LI ; Wei LIU ; Wen-yi KANG
China Journal of Chinese Materia Medica 2015;40(7):1305-1310
In order to established a method for simultaneous determination of isoquercitrin, astragaline, quercetin and kaempferol in Lysimachia clethroides, the ionic liquid 1-hexyl-3-methylimidazolium hexafluorophosphate ([HMIM]PF6) methanol was used as the ultrasound-assisted extraction solvent combing with RP-HPLC. A Purospher star RP-C1 column was used with the mobile phase of aceto- nitrile, methanol and 0. 4% phosphate acid by gradient elution at the detection wavelength of 360 nm. The flow rate was 0.7 mL x min(-1), and the column temperature was the room temperature. Under the optimized conditions, the linear ranges were 2.54 x 10(-2)-2. 54, 2.50 x 10(-2)- 2.50, 1.54 x 10(-3)-0.154, 1.49 x 10(-3)-0.149 microg for isoquercitrin, astragaline, quercetin and kaempferol, respectively. The average recoveries of the four constituents were 101.1%, 98.90%, 101.0%, 101.6%, respectively. The method was green, simple, rapid and accurate, and provided a valid method for analysis of isoquercitrin, astragaline, quercetin and kaempferol in L. clethroides.
Chemical Fractionation
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instrumentation
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methods
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Chromatography, High Pressure Liquid
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Drugs, Chinese Herbal
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analysis
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isolation & purification
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Flavonoids
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analysis
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isolation & purification
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Ionic Liquids
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chemistry
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Primulaceae
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chemistry
6.Intravascular large B-cell lymphoma: report of a case.
Guo-hua YU ; Gui-mei QU ; Wei-dong YAO ; Zhi-qiang LANG ; Wei WANG
Chinese Journal of Pathology 2009;38(7):488-489
Antigens, CD20
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metabolism
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Antigens, CD34
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metabolism
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Antineoplastic Combined Chemotherapy Protocols
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therapeutic use
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Cyclophosphamide
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therapeutic use
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Diagnosis, Differential
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Doxorubicin
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therapeutic use
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Humans
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Lymphoma, Large B-Cell, Diffuse
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drug therapy
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metabolism
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pathology
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Male
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Melanoma
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metabolism
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pathology
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Middle Aged
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Prednisone
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therapeutic use
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Vascular Neoplasms
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drug therapy
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metabolism
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pathology
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Vincristine
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therapeutic use
7.MRI evaluation on disease development of chronic brucellosis spondylitis and its value
Dong SHI ; Jie LI ; Dongkui YANG ; Zheng PAN ; Zhi LIU ; Tianhui DU ; Wei WEI ; Lei ZHENG
Chinese Journal of Zoonoses 2017;33(6):535-537,558
We evaluated the patient in the early,advanced or healing phase of the disease by MRI in the treatment of chronic brucellosis spondylitis and to guide the clinical treatment.MRI images of 40 patients with clinically diagnosed chronic brucellosis spondylitis were analyzed retrospectively.The imaging findings of early,advanced and healing patients were summarized.MRI showed abnormal signals in the vertebral body,intervertebral disc,paraspinaI and psoas muscle.It is early stage if the intervertebral space was normal,and advanced stage if combined with interverbral space stenosis.It demonstrated short T1 and short T2 signal or similar to the normal vertebral body,combined with intervertebral space stenosis,for the healing stage.According to the specific imaging manifestations of chronic brucellar spondylitis in the course of disease development,it is possible to evaluate the clinical stage of the disease and guide the rational selection of clinical treatment.
8.Multifocal eccrine angiomatous hamartoma: report of a case.
Gui-Mei QU ; Guo-Hua YU ; Wei-Dong YAO ; Zhi-Qiang LANG ; Wei WANG ; Cheng-Gong DONG
Chinese Journal of Pathology 2008;37(12):853-853
Adult
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Eccrine Glands
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pathology
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Female
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Hamartoma
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pathology
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Humans
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Sweat Gland Diseases
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pathology
9.Multilocular cystic renal cell carcinoma.
Zhi-qiang LANG ; Wei-dong YAO ; Gui-mei QU ; Lei JIANG
Chinese Journal of Pathology 2006;35(9):574-575
Carcinoma, Renal Cell
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metabolism
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pathology
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surgery
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Cytokines
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metabolism
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Diagnosis, Differential
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Humans
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Immunohistochemistry
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Kidney
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chemistry
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pathology
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surgery
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Kidney Diseases, Cystic
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metabolism
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pathology
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surgery
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Kidney Neoplasms
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metabolism
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pathology
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surgery
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Male
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Middle Aged
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Mucin-1
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metabolism
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Nephrectomy
10.Neonatal hepatoblastoma--a case report.
Zhao-hong CHEN ; Guang-jin LU ; Yu-kun HAN ; Wei-dong LI ; Zhi-jun WU
Chinese Journal of Pediatrics 2005;43(8):631-632