1.To further improve the effects of surgical treatment for hilar cholangiocarcinoma.
Xiao-ping CHEN ; Zhi-yong HUANG
Chinese Journal of Surgery 2009;47(15):1121-1122
Bile Duct Neoplasms
;
pathology
;
surgery
;
Bile Ducts, Intrahepatic
;
Cholangiocarcinoma
;
pathology
;
surgery
;
Hepatectomy
;
methods
;
Hepatic Artery
;
pathology
;
surgery
;
Humans
;
Lymph Node Excision
;
methods
;
Portal Vein
;
pathology
;
surgery
4.Hydroxyapatite nanoparticles enhance the efficacy of liposome-mediated gene-transfection into HepG2 cells and its mechanisms.
Gao-Peng LI ; Xiao-Ping CHEN ; Zhi-Yong HUANG
Chinese Journal of Oncology 2008;30(2):111-112
Cell Proliferation
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DNA
;
chemistry
;
Durapatite
;
chemistry
;
Hep G2 Cells
;
Humans
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Liposomes
;
chemistry
;
Nanoparticles
;
chemistry
;
Particle Size
;
Recombinant Proteins
;
chemistry
;
Transfection
6.The application of hepatectomy occlusion technology.
Zhi-yong HUANG ; Yang-an LIU ; Xiao-ping CHEN
Chinese Journal of Surgery 2012;50(6):485-487
Hepatectomy
;
methods
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Humans
;
Liver
;
blood supply
8.Bartter's syndrome:report of 12 cases
Xing-Duan HUANG ; Bin YAO ; Zhi-Min HUANG ; Zhi-Hong LIAO ; Jian-Ping WENG ; Guo-Liang HU ; Yan-Bing LI ;
Chinese Journal of Endocrinology and Metabolism 1986;0(03):-
Twelve cases of Bartter's syndrome were reported and reviewed retrospectively.Usually vomiting was the first sympton in children,while fatigue was common in adults.Bartter's syndrome was characteristic of hypokalemia,metabolic alkalosis,elevations of plasma renin activity,serum angiotersinⅡand aldosterone and juxtaglomerular apperatus hyperplasia.Supplementation of potassium choloride was the main manner of therapy.
9.Fourier Transform Infrared Microspectroscopy of Rat Kidney with Regard to Fa- tal Hyperthermia.
Zhi-jun WANG ; Shan-shan SHEN ; Kai-fei DENG ; Zhi-qiang QIN ; Ping HUANG ; Zhen-yuan WANG
Journal of Forensic Medicine 2015;31(4):257-261
OBJECTIVE:
To observe the chemical groups changing in rat kidney with regard to fatal hyperthermia by Fourier transform infrared microspectroscopy (FTIR-MSP) and to provide a new method to diagnose fatal hyperthermia.
METHODS:
Rats were sacrificed by hyperthermia, brainstem injury, massive hemorrhage and asphyxiation and divided into groups. The renal samples were dissected immediately after death. The data of infrared spectroscopy in glomerulus were measured by FTIR-MSP.
RESULTS:
The absorbances of 3290, 3070, 2850, 1540 and 1396 cm(-1) significantly increased (P < 0.05), and the ratios of Al650/A3290 and A1650/A1540 significantly decreased (P < 0.05) in group of hyperthermia.
CONCLUSION
FTIR-MSP can analyze the changes of chemical groups of kidney as an auxiliary diagnosis for discriminating hyperthermia with other causes of death.
Animals
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Fever/mortality*
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Fourier Analysis
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Kidney/metabolism*
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Microspectrophotometry
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Rats
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Spectroscopy, Fourier Transform Infrared/methods*
10.Neuroprotection of adenoassociated vira-mediated brain-derived neurotrophic factor on retinal ganglion cells in DBA/2J mice with glaucoma
Chu-kai, HUANG ; Ming-zhi, ZHANG ; Li-fang, LIU ; Ling-ping, CEN ; Miao-miao, HAN
Chinese Journal of Experimental Ophthalmology 2011;29(4):314-318
Background Deficiency of neurotrophic factor is associated with the damage of optic nerve in glaucoma.Reaserches showed that ectopically applied neurotrophic factor has a transient neuroprotective effect in glaucoma model,and the viral expression of adeno-associated neurotrophie factor may provide long-term supplementation of neurotrophic factor and neuroprotection in tissues.Objective The present study Was to investigate the neuroproteetive effect of adeno-associated viral(AAV)-mediated brain-derived neurotrophic factor (BDNF)on retinal ganglion cells(RGCs)in DBA/2J mice with experimental glaucoma. Methods 10 clean DBA/2J mice were administered intravitreal injection with 1 microliter of AAV-BDNF-GFP in the left eyes at the age of 6 months,and the right eyes were injected with the same volume of saline solution as control.Intraocular pressure (IOP)was measured with Tonolab in the mice every month.Retinas were obtained after 3 months for the investigation of GFP expression in RGCs using fluorescence microscopy.Immunohistochemistry Was performed by applying TUJ1 and Cy3 antibodies to identify surviving RGCs. Results The IOP of DBA/2J mice were 11.90 mmHg and 11.40 mmHg in the right eyes and left eyes,respectively,at 4 months.The IOP of DBA/2J mice began to rise at 5 months and reached its peak in 8 month-old mice.There was no statistically significant difference in IOPs between the right eyes and the left eyes from 4 month-through 9 month-old mice(t=-1.78-0.61,P=0.11-0.90).Three months after intrlavitreal injection of AAV-BDNF-GFP,GFP was positively expressed in RGCs of retinas with the expression rate of 46.33%±8.08%.The over-expression of BDNF led to more RGCs survival than the control eyes (3168.13±1319.33/mm2 vs 2024.81±796.38/mm2,t=2.75,P=0.02). Conclusion These data suggest that BDNF can exert a protective effect in DBA/2J glaucoma mice.