This study was to localize the position of spinal center of sympathetic nerve which controls the pulmonary circulation in pithed rat model. The sympathetic preganglionic fibers arising from C7-T10 spinal segments were stimulated electrically in succession. During stimulation the pulmonary vascular rcsistance(PVR) was increased in all segments tested, most significantly in C7-T4(about 28% above control value) which was obviously higher than that of Ts-T10. In constract, the systemic vascular resistance(SVR) increased more remarkably when lower segments were stimulated. The higher the stimulated spinal segments, the larger the ratio of APVR/ASVR. The data showed that the spinal center of sympathetic nerve which regulates the vasomotion of pulmonary circulation is located in the segments C7-T4.

0.05) found between two groups. Hypoxia did not stimulate ECs to release endothe-lium-derived contracting factors (EDCFs), and the contracting effects of HPEC (16.4%)and HAEC (20%) were similar to that of NPEC and NAEC. These results indicated thatEDCFs released from endothelial cells of pulmonary artery and aorta can induce contrac-tion of PSMC directly and that hypoxia did not increase the release of EDCFs from ECs.

Hypoxic vasoconstriction was studied in isolated rings of pig intrapulmonary(PA) with or without endothelium. Arterial rings were suspended in modified Krebs' bic-arbonate solution for isometric recording. Hypoxia was induced by changing the bubblinggas mixture in the chamber from, 95% O_2-5%CO_2 to 95%N_2-5%CO_2. When PA ringswere prestimulated with phenylephrine (PE, 2?10~(-6) mol/L), hypoxia could induce contrac-ctions in PA (0.86?0.09g, n=12). Removal of the endothelium decreased the hypoxicontractions of PA significantly (tension increment 0.11?0.03g, n=12, P

Adult ; Disease Susceptibility ; complications ; Female ; Hearing Loss, Noise-Induced ; complications ; Humans ; Hypertension ; etiology ; Linear Models ; Logistic Models ; Male ; Middle Aged ; Multivariate Analysis ; Noise, Occupational ; adverse effects ; Occupational Exposure ; adverse effects

Research on post-marketing Chinese medicine should be the systematic study from application to mechanism. Clinical evaluation is the basis of mechanism study, we can find the clue from clinical evaluation, then make a mechanism study to find the reason, then apply the results to clinic. So it is a virtuous circle. In order to achieve it, we cannot be limited to traditional Chinese medicine, we should form multi-disciplinary team under the direction of grand science thinking, try hard to put industry-university-research institute collaboration association to use, and if necessary, explore the new model of the whole nation system. An appropriate operation mechanism is very important.
Biomedical Research ; Medicine, Chinese Traditional

Acute Disease ; Adult ; Humans ; Male ; Middle Aged ; Organotin Compounds ; poisoning ; Retrospective Studies

AIM:To investigate the expression and the significance of VEGF-C/D in rat cornea after alkali burning as well as the role of lymphangiogenesis in the high-risk corneal transplantation rejection.
●METHODS:The model of alkali burn corneal was made. Different times corneas were taken to electron microscope for vascularization, and examined the expression of VEGF-C/D and VEGFR-3 in l, 3, 5, 7, 14, 28d. The other rat cornea after alkali burn were divided into four parts to penetrate keratoplasty, containing only blood vessels in the cornea ( group A ) , angiogenesis and lymphangiogenesis ( group B ) , lymphangiogenesis degenerating period ( group C ) , angiogenesis degenerating period ( group D ) . ln addition, there are also normal groups ( group N ) to compare the Rl values and survival time of corneal graft.
●RESULTS: Electron microscopy showed that, when the first 7d rat cornea appeared neovascularization after alkali burn, but not lymphangiogenesis. The occurrence of new blood vessels and lymphatic in 2wk. There were no obvious lymphangiogenesis in 5wk and the angiogenesis gradually subside in 8 wk. The expression of VEGF-C/D and VEGFR-3 in the corneas of rats were up-regulated in the third days after the injury, and reached its peaks at 5d. The average survival time of group N, A, B, C, D were (14.25±0.62)d, (9.35±1.02)d, (5.06±1.13)d, (8.71±0.83) d, (9. 44±1. 05)d after transplant cornea. Compared to the rest of the group, group B plant average survival time significantly shortened (P<0. 05), while compared with group B, the survival time of A, C, D groups were significantly longer (P<0. 05).
● CONCLUSION: VEGF - C/D and VEGFR - 3 are expressed significantly after corneal alkali burn. New lymphatic vessels can accelerate high - risk corneal transplantation immune rejection.

Child ; Echocardiography ; Humans ; Male ; Myocardial Infarction ; diagnosis ; Myocarditis ; diagnosis ; virology

AIM: To establish the quality standard for Yanqingsong Granule (Radix P uerariae, Rhizoma Chuanxiong, Radix Paeoniae Alba, Radix Angelicae Dahuricae, et c.). METHODS: Radix Puerariae; Rhizoma Chuanxio ng; Radix Paeoniae Alba; Radix Angelicae Dahuricae. in Yanqingsong Granule were identified by TLC, and the content of puerarin was determined by HPLC. RESULTS: Radix Puerariae; Rhizoma Chuanxio ng; Radix Paeoniae Alba; Radix Angelicae Dahuricae could be identified by TLC. P uerarin showed a good linear relationship at a ran ge of 0.1716～0.8580?g,r=0.9999. The average recovery was 97.49% (RSD= 1.77% and n=6). CONCLUSION: The methods are available with a reproducibility and ca n control the quality of this granule effectively.

BACKGROUND:Skin grafting is crucial for patients with deep burns, but limited source of autologous skin grafts is an existing difficulty.
OBJECTIVE:To investigate the effect of xenogeneic (porcine) acelular dermal matrix in the treatment of deep burn wounds and the feasibility of its application.
METHODS:Forty-one patients with deep burn were divided into two groups according to the intention of the patients, 21 cases in control group and 20 cases in observational group, folowed by autologous split-thickness skin grafting alone or combined with different (porcine) acelular dermal matrix, respectively. After 12 months of folow-up, the graft success rate at postoperative 1, 2, 3, 4 weeks and skin graft contraction rate and wound repair at postoperative 3, 6, 9, 12 months were observed and compared between two groups. Moreover, levels of inflammatory factors were detected and compared between two groups at postoperative 1, 2, 3 months.
RESULTS AND CONCLUSION: The skin graft success rates showed no difference between two groups at postoperative 1, 2, 3, 4 weeks (P > 0.05). The skin graft contraction rates also showed no difference between two groups at postoperative 3, 6, 9, 12 months (P > 0.05). After 12 months of folow-up, no serious scar hyperplasia, but soft texture appeared in the control group. In the observational group, three cases presented with local pigmentation at the early stage, but it gradualy subsided with time; no obvious scar, but only smal, point-like scar, was visible, and the repaired wound exhibited soft touch. No adverse events and death occurred in both two groups. Experimental results show that the treatment of deep burns with autologous split-thickness skin grafting combined with xenogeneic (porcine) acelular dermal matrix is safe and effective, which can improve the quality of wound healing.