1.Effect of Transcranial Low Frequency Electrical Stimulation on Contents of Monoamines in Ischemic Area of Rats with Middle Cerebral Artery Occlusion
Zhi-kuan DENG ; Jian-ning YE ; Suo-quan XU
Chinese Journal of Rehabilitation Theory and Practice 2008;14(12):1136-1139
Objective To observe the effect of transcranial low frequency electrical stimulation on the contents of monoamines in ischemic area of rats with middle cerebral artery occlusion(MCAO).MethodsPermanent MCAO model of Wistar rat was established with silk thread enveloped with polyammoniacum.The ischemic areas received various intensity of transcranial low frequency electrical stimulation for 1 hour in rats underwent 1 hour of ischemia.Then the affected tissue was processed with fluorospectrophotometry to determine the contents of dopamine(DA),noradrenalin(NE) and 5-hydroxytryptamine(5-HT).ResultsCompared with the sham-operation group,the contents of DA,NE and 5-HT in ischemic area of MCAO model rats decreased obviously(all P<0.01),while all three monoamines investigated in the sham-operation group with transcranial low frequency electrical stimulation had no significant change.In the MCAO groups stimulating with lower(10 V) and middle(30 V) intensity transcranial low frequency electrical field,the contents of DA,NE and 5-HT in ischemic area had no significant increase.But in the MCAO group stimulating with high(50 V) intensity transcranial low frequency electrical field,the contents of DA,NE and 5-HT in ischemic area increased significantly(P<0.05).ConclusionSome degree of intensity transcranial low frequency electrical field stimulation can increase the contents of DA,NE and 5-HT in ischemic area of rats subjected to MCAO.
2.Clinical study of acute low-tone sensorineural hearing loss.
Han ZHOU ; Guang-Qian XING ; Zhi-Bin CHEN ; Deng-Yuan WANG ; Xing-Kuan BU
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2005;40(5):331-334
OBJECTIVETo explore the etiology, clinical aspects, diagnosis and therapeutic strategies of acute low-tone sensorineural hearing loss (ALHL).
METHODSThirty patients (31 ears) with ALHL were selected for this study. Detailed history collection, otological examination and systematic audiological evaluations were conducted. The hearing tests included pure tone audiometry, acoustic immittance, auditory brainstem response (ABR) and otoacoustic emissions (OAE). All cases received therapeutic trial of corticosteroid for 15 days with 6 to 14 months' following-up.
RESULTSALHL mainly affected young people. Low-tone tinnitus, a sensation of ear fullness and hearing impairment were the frequent complains. Otological examinations showed normal results. Mild to moderate sensorineural hearing loss at low frequencies and type "A" tympanograms were found in all patients. Acoustic stapedial reflexes were elicited in 26 of 31 affected ears, and 14 of them had positive results on the Metz test. ABR responses were normal in all 20 tested ears. In 14 out of 20 ears, TEOAEs were absent and DPOAE grams at low frequencies (0.5, 0.75 kHz) were abnormal on the first visit. After steroid therapy, 24 ears demonstrated complete recovery, but 4 ears showed partial recovery and 3 ears unchanged. The total improvement rate was 90.3%.
CONCLUSIONSALHL patients are clinically characterized by low-tone tinnitus, aural fullness and hearing loss, which mainly involved unilateral ear. Audiological findings indicate a cochlear impairment, which only invades low frequency region. The basic pathological feature may be endolymphatic hydrops involves immune response. Conflicting data exist on whether ALHL is an independent disorder or a subtype of Meniere's disease. Ideal therapeutic strategy has not been established by now and corticosteroid is probably an effective agent.
Acute Disease ; Adrenal Cortex Hormones ; therapeutic use ; Adult ; Audiometry, Evoked Response ; Endolymphatic Hydrops ; etiology ; Female ; Hearing Loss, Sensorineural ; diagnosis ; drug therapy ; physiopathology ; Humans ; Male ; Meniere Disease ; diagnosis ; drug therapy ; physiopathology ; Middle Aged ; Otoacoustic Emissions, Spontaneous
3.Clinical study of Milan criteria for liver transplantation.
Zhong-yang SHEN ; Hong ZHENG ; Zheng-lu WANG ; Wen SHEN ; Zhi-jun ZHU ; Yong-lin DENG ; Cheng PAN ; Fu-kuan BAO
Chinese Journal of Surgery 2007;45(23):1615-1618
OBJECTIVETo investigate the value and deficiency of Milan criteria for liver transplantation in patients with hepatocellular carcinoma (HCC).
METHODSBetween December 2001 and November 2005, 125 patients underwent orthotopic liver transplantation ( OLT), who measured up Milan criteria with preoperation computerized tomography (CT) scanning. The results of pre-transplant multidetector CT scan and post-transplant pathology were retrospectively analyzed, and survival rates were compared.
RESULTSPathology examination demonstrated that 97 cases met Milan criteria (77.6%), 26 cases exceeded Milan criteria,and the other 2 cases were diagnosed as nodular cirrhosis. The 1-,2-,3-,4- and 5-year survival rates for those met pre-transplant multidetector CT scanning pre-transplant met Milan criteria vs. those met post-transplant pathology post-transplant criteria were 92.0% vs. 92.8%, 87.2% vs. 90.7%, 86.4% vs. 89.7%, 86.4% vs. 89.7%, and 86.4% vs. 89.7%, respectively. There was no statistic significant difference (P > 0.05). The 1-,2-,3-,4- and 5-year survival rates were 73.0%, 65.4%, 61.5%, 61.5% and 61.5%, for those pathology exceed Milan criteria respectively. The difference between this group and each of the above two were statistically significant (P < 0.05).
CONCLUSIONSThe prognosis of OLT for HCC is good for those met Milan criteria by pre-transplant multidetector CT. Factors leading to poor prognosis such as portal vein tumor thrombi and lymphatic metastasis should be accurately evaluated avoiding for misjudgement.
Adult ; Aged ; Carcinoma, Hepatocellular ; diagnostic imaging ; pathology ; surgery ; Female ; Follow-Up Studies ; Humans ; Liver Neoplasms ; diagnostic imaging ; pathology ; surgery ; Liver Transplantation ; methods ; mortality ; standards ; Male ; Middle Aged ; Retrospective Studies ; Survival Analysis ; Survival Rate ; Tomography, Spiral Computed
4.Application of Montreal Cognitive Assessment in screening mild cognitive impairment in elderly patients
Hai-Yuan LI ; Yan-Ping WANG ; Shao-Kuan HUANG ; Shao-Qing YANG ; Sheng-Qiang CHEN ; Yong-Ping DENG ; Zhi-Chao CHEN
Chinese Journal of Neuromedicine 2009;8(4):376-379
Objective To assess the value of Montreal Cognitive Assessment(MoCA)in screening mild cognitive impairment(MCI)in elderly patients.Methods Thirty-two elderly patients with MCI and 50 healthy elderly subjects were examined with neuropsychological tests using Mini-mental State Examination(MMSE)and the MoCA. Results With a cut-off score of 26,the MoCA had a sensitivity of 96.87%and specificity of 76%for detecting MCI,and the sensitivity and specificity of MMSE were 56.25%and 96%,respectively.For the MoCA sub-items,the elderly patients with MCI had significantly lower scores than the healthy elderly in all the cognitive function measures (P<0.05)except for abstraction and fixed orientation.In MMSE sub-items,significant differgnces were found between the two groups in only calculation/attention and delayed recall(P<0.05).Conclusions The MoCA is a highiy sensitive scale for screening MCI in elderly patients,which allows comprehensive assessment of the cognitive function of MCI patients and is applicable in detecting MCI in patients with normal MMSE results.
5.Epidemiologic study on hearing impairment and ear diseases in old people.
Cheng LIU ; Xing-Kuan BU ; Guang-qian XING ; Ling ZHOU ; Xia XU ; Deng-yuan WANG ; Zhi-bin CHEN ; Han ZHOU ; Hui-qin TIAN ; Xiao-lu LI ; Ling LU ; Xiao-nian ZHAO ; Fang-li LI ; Chang-qiang TAN
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2006;41(9):661-664
OBJECTIVETo investigate the prevalence of hearing impairment and ear diseases in old people and provide scientific data for drawing up the prevention and treatment strategies.
METHODSUsing the probability proportion to size (PPS) method, 1261 people over 60 years were investigated in 40 clusters in Jiangsu Province with the WHO protocol.
RESULTSThe prevalence of hearing impairment was 58.1% (the standardized rate: 59.5% in the whole country, 60.9% in Jiangsu province). Degrees of hearing impairment were mild (33.1%), moderate (17.8%), severe (5.9%) and profound (1.3%). The prevalence of hearing disability was 25.0% (the standardized rate: 26.6% in the whole country, 28.1% in Jiangsu province). There were significant difference of the prevalence between male and female, as well as urban and rural, and different ages. The prevalence of the ear diseases was auricle malformation (0.2%), wax (1.7%), otitis externa (0.1%), fungi (0.5%), serous otitis media (1.2%), chronic suppurative otitis media (1.6%), dry perforation of tympanic membrance (2.3%). The causes of hearing impairment were ear diseases (2.9%), non-infectious condition (92.6%), genetic condition (0.3%) and undetermined causes (4.2%). Of which, 31.1% of persons needed hearing aids while 2.3% of persons needed medicine treatment, but 0.9% of persons needed non-urgent surgery and 1.0% of persons needed other treatment.
CONCLUSIONSThe prevalence of hearing impairment and disability in the old rised obviously than the last investigation in 1987. It was a heavy burden for social development in China. The government and the whole society should take more concern about the problem. The scientific strategies of prevention and treatment were urgently needed and implemented.
Aged ; Aged, 80 and over ; Audiometry, Pure-Tone ; China ; epidemiology ; Ear Diseases ; epidemiology ; Female ; Hearing Loss ; epidemiology ; Humans ; Male ; Middle Aged ; Prevalence
6.Construction of a human cervical spine with bilateral vertebral artery fluid-solid coupling model.
Hui-hao WANG ; Zhi-bi SHEN ; Zhen DENG ; Kuan WANG ; Hong-sheng ZHAN ;
Journal of Zhejiang University. Medical sciences 2015;44(2):131-137
OBJECTIVETo construct a human cervical spine with bilateral vertebral artery fluid-solid coupling model.
METHODSHelical CT images under the principle of reverse engineering and meshed in finite element model(FEM) related software were used to establish a human cervical spine with bilateral vertebral artery fluid-solid coupling model. In the process of modeling of vertebral body, vertebral artery, ligament, intervertebral disc, cartilage and endplate large anatomic data and cadaver experiments results were referenced. From the morphology and function the simulation of model with real physiological status was tested.
RESULTSThe study showed that the stress concentration on the surface of vertebral body and the blood wall of the bilateral vertebral artery, and the result of the volume flow rate-time curve of bilateral vertebral artery of the model were consistent with the published literatures. This model was well consistent with the clinical phenomenon.
CONCLUSIONThe three-dimensional FEM of the human cervical spine established by the introduced method has been effectively verified. The modeling method would provide a new tool for research on the cervical spine biomechanics.
Biomechanical Phenomena ; Cadaver ; Cervical Vertebrae ; anatomy & histology ; Finite Element Analysis ; Humans ; Intervertebral Disc ; Models, Anatomic ; Tomography, Spiral Computed ; Vertebral Artery ; anatomy & histology