1.Research progress on mitochondrial oxidative stress and corresponding targeted delivery systems in Alzheimer's disease
Ling-ling ZHOU ; Kang QIAN ; Peng YANG ; Qi-zhi ZHANG
Acta Pharmaceutica Sinica 2022;57(6):1630-1640
Mitochondrial oxidative stress has been recognized as a preliminary and critical factor that aggravates the pathological cascade of Alzheimer's disease, which induces the production of
2.Study on accumulation law of peimine in different Fritillaria thunbergii cultivar.
Yan-nan ZHANG ; Kang-cai WANG ; Xiao-qian ZHANG ; Zhi-wei CUI ; Qian WANG
China Journal of Chinese Materia Medica 2015;40(3):421-423
In order to study the accumulation of Fritillaria thunbergii cultivar, peimine content in Xiaye, Kuanye, Duozi and Xiaosanzi bulbs of different sizes and parts was determined by HPLC-ELSE. The results indicated that the peimine content varied significantly with the cultivar type, the size and part of bulb. The distribution laws of peimine were as follow: Xiaosanzi > Duozi > Xiaye > Kuanye, small-size bulb > big-size bulb, core bud > scale. The peimine yield per plant in Duozi was the highest.
Cevanes
;
analysis
;
Chromatography, High Pressure Liquid
;
Fritillaria
;
chemistry
;
growth & development
3.Advance on chemical compounds of Ainsliaea genus.
Fa-jin FENG ; Zhi-ling XU ; Qian-jun ZHANG ; Zhen-hua YIN ; Wen-yi KANG
China Journal of Chinese Materia Medica 2015;40(7):1244-1251
Plants in Ainsliaea genus, belongs to Compositae family, are traditional Chinese medicine and widely used in folk. These plants contain various types of chemical components, and main components are sesquiterpene lactone and its glycosides. In addition, there are triterpenoids, flavonoids, steroids, phenolic acid, long chain fatty acid and volatile oils. Recently, much attention has been payed to varlous research of A. fragrans. This paper reviewed and summarized the chemical components to provide the theoretical basis for the use of Ainsliaea.
Asteraceae
;
chemistry
;
Drugs, Chinese Herbal
;
chemistry
;
pharmacology
;
Molecular Structure
4.Effect of low-temperature vernalization on metabolism of carbon and nitrogen of Isatis indigotica.
Qian-Qian LIU ; Kang-Cai WANG ; Qian WANG ; Zhi-Wei CUI
China Journal of Chinese Materia Medica 2013;38(10):1484-1488
OBJECTIVEThe aim of the present study is to investigate the effect of low temperature vernalization on metabolism change of carbon and nitrogen of Isatis indigotica.
METHODThe Yunnan and Beijing I. indigotica seedlings with six leaves were vernalized at 4 degrees C for 25 days, and the metabolism indicators of carbon and nitrogen were measured.
RESULTThere appeared a dramatic increase in the soluble sugar content, reducing sugar content and soluble protein content in response to the low temperature, after termination of vernalization it reached the maximum, however, starch and total nitrogen concentration decreased significantly, after termination of vernalization it reached the minimum.
CONCLUSIONThe high C/N value can promote the low temperature vernalization of I. indigotica.
Carbon ; metabolism ; Cold Temperature ; Isatis ; metabolism ; Nitrogen ; metabolism
5.Amifostine in treatment of five patients with myelodysplastic syndrome.
Hong-lan QIAN ; Zhi-jian SHEN ; Xu-dong HU ; Yong-xian HU ; Kang YU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2006;24(10):622-623
Adult
;
Aged
;
Amifostine
;
therapeutic use
;
Benzene
;
poisoning
;
Female
;
Humans
;
Male
;
Middle Aged
;
Myelodysplastic Syndromes
;
chemically induced
;
drug therapy
6.Effect of EPHA2-siRNA plasmid on biological behavior of human osteosarcoma cells in vitro.
Li-zhi ZHANG ; Xuan-song CAI ; Zhi-kang QIAN ; Jiong MEI ; Xiao-hui MA ; Huang-yuan HUANG ; Wei-da HUANG
Chinese Journal of Oncology 2007;29(8):566-569
OBJECTIVETo investigate the role of EPHA2 in regulating apoptosis, proliferation and vasculogenic mimicry of osteosarcoma cells, by gene silencing through RNA interference.
METHODSEPHA2-siRNA plasmids were achieved by gene cloning. The plasmids were transfected into human osteosarcoma cells (MG63). The expression level of EPHA2 protein was measured by Western blotting. The proliferation, apoptosis and vasculogenic mimicry features of osteosarcoma MG63cells were assessed by light microscopy, MTIP assay, flow cytometry, annexin V-FITC/PI and HE staining, respectively.
RESULTSThe EPHA2-siRNA plasmid was confirmed by DNA sequencing. After treatment with Sequence-specific siRNA targeted EPHA2, the protein level of the transfected group decreased significantly. As compared to non-siRNA transfected cells, the transfected group showed lower proliferation, higher and earlier apoptosis and less osteosarcoma-generated vasculogenic mimicry.
CONCLUSIONEPHA2 gene may be involoved in apoptosis and proliferation of osteosarcoma cells, and may be necessary for vasculogenic mimicry. Down-regulation of EPHA2 expression by sequence-specific siRNA may be considered as a new option in the treatment of EPHA2 over-expressing cancer including osteosarcoma in future.
Apoptosis ; Bone Neoplasms ; metabolism ; pathology ; Cell Line, Tumor ; Cell Proliferation ; Humans ; Neovascularization, Pathologic ; pathology ; Osteosarcoma ; metabolism ; pathology ; Plasmids ; RNA Interference ; RNA, Small Interfering ; genetics ; Receptor, EphA2 ; genetics ; metabolism ; Transfection
7.Establishment of genotyping method for human platelet antigens of HPA-15 system by PCR-SSP.
Yue-Kang CHEN ; Da-Cheng LI ; Da-Ming WANG ; Qian LI ; Zhi-Hui DENG
Journal of Experimental Hematology 2008;16(1):185-188
This study was aimed to establish the reliable genotyping method of human platelet antigens of HPA-15 system by PCR-SSP and to use this assay in the further HPA genotyping of volunteer platelet donors. 3 sequence-specific primers recommended by the 11th Platelet Genotyping and Serology Workshop on behalf of International Society of Blood Transfusion (ISBT) were synthesized. The concentration of each primer pair, the concentration of Mg(2+) and the PCR conditions were adjusted to optimize the conditions so that HPA-15 system could be specific amplified. The accuracy and reliability of the developed assay was evaluated and confirmed by typing the coded DNA samples provided by the 11th Platelet Genotyping and Serology Workshop. As a parallel control, a total of 50 volunteer platelet donors in Shenzhen were genotyped by both our assay and the G&T commercial kit at HPA-15 system. 10 coded samples distributed by the 11th Platelet Genotyping and Serology Workshop were genotyped by established PCR-SSP method. The results showed that a concordance rate of 100% was observed between the results obtained by established PCR-SSP method and the results provided by ISBT report. The HPA gene frequencies observed in 50 randomly-selected platelet donors in Shenzhen were 0.5100 and 0.4900 for HPA-15a and HPA-15b respectively. In conclusion, PCR-SSP assay established in our study provides a simple, rapid and accurate method for HPA-15 system genotyping, which assay is suitable for routine clinical HPA genotyping and shows a broad prospect in its further applications.
Antigens, CD
;
genetics
;
immunology
;
Antigens, Human Platelet
;
genetics
;
GPI-Linked Proteins
;
Genotype
;
Humans
;
Isoantigens
;
genetics
;
immunology
;
Neoplasm Proteins
;
genetics
;
immunology
;
Polymerase Chain Reaction
;
methods
;
Polymorphism, Single-Stranded Conformational
8.Value of galactose-deficient IgA1 in the early diagnosis of Henoch-Schönlein purpura nephritis in children.
Zhi-Juan KANG ; Bo LIU ; Zhi-Hui LI ; Cui-Rong DUAN ; Tian-Hui WU ; Man XUN ; Yi ZHANG ; Yun-Feng DING ; Ru-Qian FU
Chinese Journal of Contemporary Pediatrics 2019;21(2):172-175
OBJECTIVE:
To explore the value of galactose-deficient IgA1 (Gd-IgA1) in the early diagnosis of Henoch-Schönlein purpura nephritis (HSPN) in children.
METHODS:
A total of 67 hospitalized children who were definitely diagnosed with HSPN between January and April 2018 and 58 hospitalized children with Henoch-Schönlein purpura (HSP) were enrolled in the study. Twenty children undergoing routine physical examinations served as controls. The levels of serum and urine Gd-IgA1 were determined using ELISA. The receiver operating characteristic curve was used to analyze the value of serum Gd-IgA1 and urine Gd-IgA1/urine creatinine ratio in the diagnosis of HSPN.
RESULTS:
The level of serum Gd-IgA1 and urine Gd-IgA1/urine creatinine ratio in children with HSP or HSPN were significantly higher than those in healthy control group (P<0.01), with a significantly greater increase observed in children with HSPN (P<0.01). Serum Gd-IgA1 ≥1 485.57 U/mL and/or urine Gd-IgA1/urine creatinine ratio ≥105.74 were of favorable value in the diagnosis of HSPN. During the six-month follow-up of the 49 children with HSP, the incidence of HSPN was 47% (23/49), which included a 100% incidence in children with serum Gd-IgA1 ≥1 485.57 U/mL and a 73% incidence in children with urine Gd-IgA1/urine creatinine ratio ≥105.74.
CONCLUSIONS
Serum and urine Gd-IgA1 is of favorable clinical value in the early diagnosis of HSPN.
Child
;
Early Diagnosis
;
Galactose
;
Glomerulonephritis, IGA
;
Humans
;
Immunoglobulin A
;
Purpura, Schoenlein-Henoch
10.Effect of N-acetyl-cysteine and depakine pretreatment on ferrous chloride-induced membrane potential and peroxidate changes in rat cortex neurons.
Yuan-xiang LIN ; Ru-xiang XU ; Xiao-dan JIANG ; De-zhi KANG ; Yi-quan KE ; Mou-xuan DU ; Ying-qian CAI ; Ling-sha QIN
Journal of Southern Medical University 2006;26(4):448-451
OBJECTIVETo investigate the effect of N-acetyl-cysteine (NAC) and depakine (DP) on the changes of membrane potential and peroxidate in rat cortex neurons exposed to ferrous chloride (FeCl(2)).
METHODSCultured cortex neurons of newly born SD rats were randomly divided into control group (PBS group), model group (FeCl(2) group), NAC pretreatment group (NAC group), DP pretreatment group (DP group) and NAC+DP pretreatment group (NAC+DP group). In the latter three groups, NAC (0.08 mg/ml) and DP (0.1 mg/ml) were added in the cell culture 2 and 3 h before FeCl(2) (1 mmol/L) exposure, respectively. After exposure to FeCl(2), the membrane potential of the neurons was detected with fluorescent dye DiBAC4(3) (bis-(1,3-dibutylbarbituric acid) trimethine oxonol), and the peroxidate level with 2,7-dichlorofluorescin diacetate (H(2)DCF) by laser confocal scanning microscope (LCSM) and nuclear factor-KappaB (NF-KappaB) level with immunocytochemistry.
RESULTSCompared with FeCl(2) group, the expression of NF-KappaB and peroxidate level in the neurons were decreased significantly in NAC and NAC+DP groups (P<0.01), but not in DP group (P>0.05). FeCl(2) depolarized the membrane potential and increased the expression of NF-KappaB in the neurons. Compared with FeCl(2) group, significant changes in the membrane potential were observed in DP and NAC+DP groups (P<0.01) but not in NAC or PBS group (P>0.05).
CONCLUSIONBoth NAC and DP can protect the neurons from FeCl(2)-induced damage but through different pathways, and their combined use can significantly alleviate neuronal damages due to FeCl(2) exposure. Antioxidants such as NAC in combination with antiepileptic drugs may produce favorable effect in prevention and treatment of posttraumatic epilepsy.
Acetylcysteine ; pharmacology ; Animals ; Animals, Newborn ; Cells, Cultured ; Cerebral Cortex ; cytology ; metabolism ; physiopathology ; Female ; Ferrous Compounds ; pharmacology ; Male ; Membrane Potentials ; drug effects ; Neurons ; cytology ; metabolism ; physiology ; Neuroprotective Agents ; pharmacology ; Peroxides ; metabolism ; Rats ; Rats, Sprague-Dawley ; Valproic Acid ; pharmacology