1.Erythropoietinprotein and its mRNA expression in rats cortical neurons following hypoxia
Jun LI ; Tao TAO ; Zhehua ZOU ; Jian XU ; Kaijian LUO ; Zhi LIU
Chinese Journal of Neurology 2014;47(4):254-257
Objective To observe erythropoietin (EPO) and its mRNA expression changes in rats cortical neurons when suffering hypoxia and investigate the endogenous EPO protective effect of hypoxia neuronal cells.Methods Cultured cortical neurons were prepared from hypoxia rats and divided into control,hypoxia 12,24,48,72 h group.Using immunohistochemistry,reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot,we observed EPO and its mRNA expression in cells,and also observe the culture medium containing lactate dehydrogenase to evaluate the activity of neurons in the whole process.Results Immunohistochemistry,RT-PCR and Western blot analysis showed:the EPO (20.79 ± 2.98) and its mRNA (0.78 ± 0.05) at 12 h had a basic expression in hypoxia group,compared with the control group (EPO:17.12 ± 1.99; mRNA:0.39 ± 0.05),and the difference was statistically significant (t =2.51,P < 0.05 ; t =13.51,P < 0.01) ; the strongest expression was observed at 48 h (EPO:28.88 ± 3.41,mRNA:1.45 ± 0.07),the difference was statistically significant (t =7.29,P < 0.01 ; t =33.24,P < 0.01) ; and neuronal activity was strongest.Lactate dehydrogenase activity was significantly decreased after hypoxia 72 h,and also a statistically significant difference was found between the groups at each point.Conclusions The EPO and EPO mRNA expression are increased after hypoxia in neuron cells,and may enhance the activity of neurons.Our study suggests that EPO might be involved in the development process of neuronal hypoxia and play an important role in neuronal hypoxia process.
2.Research and application of incorporating innovative research into undergraduate training
Yong-hong DU ; Jian-zhong ZOU ; Zhi-biao NG WA ; Jin BAI ; Jia-zhen YAN
Chinese Journal of Medical Education Research 2011;10(7):777-779
To introduce scientific research innovation into undergraduate education, cultivating innovative talents has been an urgent mission of current higher education. This article reviewed our experience, with the introduction of producing-studying-researching platform built on original innovative achievements of Chongqing medical university,of training physical medicine physician to be practical talents of large-scale diagnostic and therapeutic medical equipments, and was aimed to explore introducing producing-studying-researching platform into undergraduate education as well as improve personnel training quality of undergraduates.
3.The surgical procedure and clinical results of Stanford A aortic dissection
Zhi-Yun XU ; Liang-Jian ZOU ; Zhi-Gang SONG ; Jibin XU ; Fanglin LU ; Lin HAN ; Zhinong WANG ; Feng ZHAO ;
Chinese Journal of Thoracic and Cardiovascular Surgery 1995;0(05):-
Objective To evaluate the methods and consequences of surgical technique in the treatment of Stanford A aortic dissection.Methods 108 patients with type Standford A aortic dissection underwent surgery in our study,including urgent surgery in 53 and selective surgery in 55.The operation was performed under deep hypothennic circulatory arrest (DHCA) in 85 cases.Surgical procedures included ascending and semi arch replacement or total arch replacement (some cases combined with stented graft implanted into the descending aorta),"elephant trunk" procedure.Concomitant procedures included repair of intimal tear in arch or descending aorta,Bentall procedure,aortic valve replacement,Cabrol or modified Cabrol procedure,aortic valvuloplasty,mitral valvuloplasty or mitral valve replacement,tricuspid valvuloplasty and CABG.Results In-hospital mortality was 6.5% (7 of 108 patients).The mor- tality was 7.5% (4 of 53 patients) in urgent surgery group and in elective surgery group was 5.4% (3 of 55 patients).Ninety six percent survived patients were followed up for 1 month to 13.3 years [mean (3.2?1.3) years] and 2 deaths occurred during the fel- low-up period.3 patients underwent re-operatian.Conclusion The choice of surgical procedures depend on the location of intimal tear for Stanford A aortic dissection.The better operative effects can be expected with proper surgical indication,perfecting surgical technique,and enhancing postoperative treatment.
4.Chemical constituents in higher polar substances from Desmodium caudatum.
Dan ZHU ; Di WANG ; Guang-Hui WANG ; Zhi-Jian GUO ; Xiu-Hong ZOU ; Ting LIN ; Hai-Feng CHEN
China Journal of Chinese Materia Medica 2014;39(16):3112-3116
In this study the chemical constituents of the higher polar sustances from Desmodium caudatum were investigated.The compounds were isolated by using column chromatographies over silicagel, polyamide, ODS, Sephadex LH-20, and preparative HPLC. The structures of these compounds were identified on the basis of NMR and MS spectra. Thirteen compounds were obtained and their structures were identified as vanillin(1), loliolide(2), indole-3-carboxaldehyde(3), salicylic acid(4), swertisin(5), saccharumoside C(6), isosinensin (7), kaempferol 3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside (8), isovitexin (9), vitexin (10), nothofagin(11), resveratroloside (12), and 2"-α-rhamnopyranosyl-7-O-methylvitexin (13). Except for compound 5, the remaining compounds were isolated from D. caudatum for the first time. Compounds 2, 3, 6-8, 11-13 were separated from the genus Desmodium for the first time.
Apigenin
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chemistry
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isolation & purification
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Drugs, Chinese Herbal
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chemistry
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isolation & purification
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Fabaceae
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chemistry
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Molecular Structure
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Saponins
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chemistry
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isolation & purification
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Spectrometry, Mass, Electrospray Ionization
5. Application of ICG fluorescence imaging in surgical treatment of recurrent hepatocellular carcinoma
Xiong-feng ZOU ; Zhi-xiang JIAN
Chinese Journal of Practical Surgery 2019;39(10):1040-1041
The recurrence rate of primary hepatocellularcarcinoma is very high,and repetitive resection is still the besttreatment for operable recurrent hepatocellular carcinoma.Indocyanine green(ICG) has been widely used andpopularized in resection of hepatocellular carcinoma,providesmore possibilities and diversity for the surgical treatment ofrecurrent hepatocellular carcinoma. Combined withpreoperative imaging,three-dimensional imaging technologyand intraoperative ultrasound,ICG can effectively improve theradical and accuracy of the operation for recurrent hepatocellular carcinoma. ICG-guided hepatectomy isappropriate for the new concept and technical system of liversurgery advocated by modern medical model, and realizesfunctional and anatomical hepatectomy for recurrenthepatocellular carcinoma.
6.Detection of cytokine expression patterns in the peripheral blood of patients with acute leukemia by antibody microarray analysis.
Qing, LI ; Mei, LI ; Yao-hui, WU ; Xiao-jian, ZHU ; Chen, ZENG ; Ping, ZOU ; Zhi-chao, CHEN
Journal of Huazhong University of Science and Technology (Medical Sciences) 2014;34(2):176-80
The cytokines of acute leukemia (AL) patients have certain expression patterns, forming a complex network involved in diagnosis, progression, and prognosis. We collected the serum of different AL patients before and after complete remission (CR) for detection of cytokines by using an antibody chip. The expression patterns of cytokines were determined by using bioinformatics computational analysis. The results showed that there were significant differences in the cytokine expression patterns between AL patients and normal controls, as well as between acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). In confirmatory test, ELISA revealed the expression of uPAR in AL. Moreover, the bioinformatic analysis showed that the differentially expressed cytokines among the AL groups were involved in different biological behaviors and were closely related with the development of the disease. It was concluded that the cytokine expression pattern of AL patients is significantly different from that of healthy volunteers. Also, differences of cytokine expression patterns exist between AML and ALL, and between before and after CR in the same subtype of AL, which holds important clinical significance for revealing disease progression.
7.Research on the influence of 12-week basic load resistance training on the physical fitness of flight students in an aviation school.
Zhe JI ; Hai-Tao ZHOU ; Zhi-Kang ZOU ; Xian GUO ; Xin ZHANG ; Hui CAO ; Zhi-Dong JIANG ; Xiang REN ; An-Li WANG ; Jian-Min CAO
Chinese Journal of Applied Physiology 2021;37(5):519-522
8.Aortic valve replacement: the experiences of 1026 cases.
Bao-ren ZHANG ; Zhi-yun XU ; Liang-jian ZOU ; Er-song WANG ; Jian-zhou XING ; Wei-yong YU ; Zhi-nong WANG
Chinese Journal of Surgery 2008;46(4):259-262
OBJECTIVETo study the changes in pathogenic causes and the prognosis of aortic valve replacement (AVR).
METHODSThe clinical data of 1026 patients undergoing AVR from December 1980 to December 2006 were analyzed retrospectively. The mortality, morbidity, changes in pathogenic causes and risk factors were analyzed.
RESULTSThe postoperative mortality and complication morbidity were 4.3% and 10.6% respectively within 30 days followed operation. Main causes of operative death were heart failure, multi organ failure and endocarditis. The major risk factors for operative death were left ventricle ejection fraction less than 0.4, endocarditis, valve regurgitation and emergency operation before AVR. Late mortality was 0.54% patient-year (3.4%), most of whom died of heart failure, endocarditis and arrhythmias. Patients underwent reoperation 0.22% patient-year (1.4%), with the causes of endocarditis and perivalvular fistula.
CONCLUSIONSMorbidity of rheumatic damage in aortic valve has decreased, while valve degeneration has increased gradually in the recent years. Avoiding prosthesis-patient mismatch, good postoperatively guide and prevention of endocarditis can improve the prognosis of AVR.
Adolescent ; Adult ; Aged ; Aortic Valve ; surgery ; Female ; Follow-Up Studies ; Heart Valve Diseases ; surgery ; Heart Valve Prosthesis Implantation ; methods ; mortality ; statistics & numerical data ; Humans ; Male ; Middle Aged ; Postoperative Complications ; epidemiology ; mortality ; Prognosis ; Retrospective Studies ; Risk Factors ; Treatment Outcome
9.Investigation of GSTT1, GSTM1 and NQO1 genes polymorphisms in myelodysplastic syndromes.
Lin YANG ; Xu ZOU ; Mei-rong ZHANG ; Yue ZHANG ; Yu-shu HAO ; Zhi-jian XIAO
Chinese Journal of Hematology 2005;26(11):674-677
OBJECTIVETo investigate the relationship between GSTM1, GSTT1 and NQO1(C609T) genotypes and myelodysplastic syndromes (MDS) susceptibility and chromosome abnormalities.
METHODSGSTT1, GSTM1 and NQO1(C609T) genotypes were detected in 52 MDS patients and 241 unrelated controls by PCR or PCR-RFLP.
RESULTSThe incidence of GSTT1 and GSTM1 null genotype was significantly increased in MDS patients as compared with controls (P = 0.001 and P < 0.001, respectively). In individuals with GSTT1 and GSTM1 null genotype, the odds ratios for MDS risk were elevated to 2.873 (95% CI: 1.491-5.537) and 3.591 (95% CI: 1.717-7.508), respectively. A significantly increased frequency of GSTT(1) null genotype among MDS patients with normal karyotype and increased frequency of GSTM1 null genotype among MDS patients with chromosome abnormalities were found as compared to controls (OR = 5.336, P = 0.005 and P = 0.003, OR = 3.740, respectively). There was no difference in the incidence of NQO1(C609T) genotypes between MDS patients and controls.
CONCLUSIONDetermination of the GSTM1 and GSTT1 genotypes may be used as a stratification marker to predicate high-risk individuals for MDS.
Adolescent ; Adult ; Aged ; Female ; Gene Frequency ; Genetic Predisposition to Disease ; Genotype ; Glutathione Transferase ; genetics ; Humans ; Male ; Middle Aged ; Myelodysplastic Syndromes ; genetics ; NAD(P)H Dehydrogenase (Quinone) ; genetics ; Polymorphism, Genetic ; Polymorphism, Restriction Fragment Length
10.Role of Baicalein in the regulation of proliferation and apoptosis in human myeloma RPMI8226 cells.
Qiu-bai LI ; Yong YOU ; Zhi-chao CHEN ; Jian LÜ ; Jing SHAO ; Ping ZOU
Chinese Medical Journal 2006;119(11):948-952
Apoptosis
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drug effects
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Arachidonate 12-Lipoxygenase
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analysis
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physiology
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Cell Cycle
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drug effects
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Cell Line, Tumor
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Cell Proliferation
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drug effects
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Enzyme Inhibitors
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pharmacology
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Flavanones
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pharmacology
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Humans
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Lipoxygenase Inhibitors
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Multiple Myeloma
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drug therapy
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pathology