Objective To explore the relationship of neuron injury and its severity with the cause and the duration of convulsion Methods Patients were divided into 5 groups or 3 groups according to the causes of convulsion or duration of convulsion in children The levels of neuron specific enolase(NSE) and nitrogen monoxide and nitricoxide synthase(NO/NOS) in serum and cerebrospinal fluid(CSF) were tested by ELISA and spectrophotography at different time points after convulsion in children Results The levels of NSE and NO/NOS in serum and CSF of all objective groups except the febrile convulsion group were apparently higher than those in control group( t =15 4～47 7, P

Bile Duct Neoplasms ; pathology ; surgery ; Bile Ducts, Intrahepatic ; Cholangiocarcinoma ; pathology ; surgery ; Hepatectomy ; methods ; Hepatic Artery ; pathology ; surgery ; Humans ; Lymph Node Excision ; methods ; Portal Vein ; pathology ; surgery

Objective To detect the variation of neuron specific enolase (NSE) and D dimer in blood from neonates with hypoxic ischemic encephalopathy (HIE), and to explore the correlation of NSE and D dimer with brain damage. Methods NSE and D dimer levels in blood from neonates with HIE were measured by ELISA and turbidimetry before and after therapy. Results Blood NSE and D dimer levels in all objective cases were significantly higher than those in the control cases at 1 d after HIE and 5 d after therapy ( t =2.18-15.17, P

Bile Duct Neoplasms ; surgery ; Bile Ducts, Intrahepatic ; Cholangiocarcinoma ; surgery ; Humans

Adult ; Bronchoalveolar Lavage ; methods ; Humans ; Male ; Pulmonary Surfactants ; therapeutic use ; Silicosis ; therapy

Hepatectomy ; methods ; Humans ; Liver ; blood supply

Objective To establish brain hypoxic-ischemic (HI) model using postnatal day 3(P3) SD rats and evaluate the apoptotic neuronal cells in cerebral cortex and neurofunctional development.Methods The P3 rats were randomly divided into HI group (n=35) and control group(n=18).HI was induced in P3 rats with right carotid artery ligation followed by 2.5 h hypoxia in 60 mL?L-1 oxygen at 37 ℃.The injury of neural cells in the cerebral cortex was evaluated by tumor necrosis factor receptor-1(TNF-R1),Caspase-3 immunostaining and Hematoxylin-Eosin (HE) staining in 24 h and 7 d after HI,respectively.Furthermore,the neurofunctional development was evaluated by negative geotaxis reflex and eye opening time.The data were analyzed by SPSS 12.0 software.Results Caspase-3 and TNF-R1 positive cells were abundant in the ipsilateral cortex at 24 h after HI,compared with contralateral part and control group(P

AIM: To observe the effects of scleral buckling surgery for retinal detachment under microscope and under indirect ophthalmoscope.
METHODS: Forty - six patients ( 46 eyes ) with rhegmatogenous retinal detachment were retrospectively analyzed. Twenty-six patients received surgeries under microscope was retrospectively analyzed and compared to that of 20 patients who received surgeries under indirect ophthalmoscope by the same operator.
RESULTS: No statistical difference was found in patients' operation method choice, including condensation reaction reattachment situation and the retina reset operation at a time ( P> 0. 05 ). The best corrected visual acuity of microscopic group after 1mo of operation was better than before operation, and the improving rate of vision after operation was higher than that of the traditional group (P<0. 05).
CONCLUSION:The curative effect of external approach microsurgery has the similar efficacy with the conventional external approach surgery, and gets better visual acuity.

Objective To explore the relationships between erythropoietin( EPO), endothelin - 1 (ET - 1) and perinatal anoxia. Method ELISA was used to test cord blood EPO and ET-1 in 54 high risk neonates as subjects and 14 healthy neonates as controls.Results The cord blood EPO levels in amniotic fluids turbid Ⅲ degree group and group eclampsia/pre - eclampsia were higher than those in control group (t= 4.0842,3 680 allP

Cell Proliferation ; DNA ; chemistry ; Durapatite ; chemistry ; Hep G2 Cells ; Humans ; Liposomes ; chemistry ; Nanoparticles ; chemistry ; Particle Size ; Recombinant Proteins ; chemistry ; Transfection