Objective To investigate the association between serum N-terminal pro brain natriuretic peptide(NT-ProBNP) and creatine kinase myocardial(CK-MB),cardiac troponin I(cTnI) in children who were diagnosed as viral myocarditis(VMC),and to explore the clinical significance.Methods Fifty children with VMC were selected as VMC group who were further divided into cTnI(+)group and cTnI(-)group.Fifteen healthy children were selected as healthy control group.The levels of myocardial enzyme of the 2 groups were tested by automatic biochemical analyzer and level of NT-proBNP was detected by specific NT-proBNP enzymelink immunosorbent assay(ELISA) kit.SPSS 13.0 software was used to analyze the data.Results 1.There were 23 cases with positive cTnI and 27 cases with negative cTnI.The levels of NT-proBNP in VMC group including cTnI positive and cTnI negative were significantly higher than those in healthy control group (P0.05).2.In the group with positive cTnI,the level of NT-proBNP was correlated with cTnI.The coefficient of determination was 0.17.3.In the group with negative cTnI,the level of NT-proBNP was correlated with CK-MB.The coefficient of determination was 0.34.NT-proBNP presented a increasing trend during interval of 25-100 U?L-1 and 175-255 U?L-1 of CK-MB.Conclusions The levels in the serum NT-proBNP in VMC children were higher than those in healthy children.Serum detection NT-proBNP may be applied as a new approach in diagnosis of VMC in children.

BACKGROUND: The therapeutic effect of mild hypothermia in the treatment of severe brain injury has been recognized in spite of the poor understanding of its mechanism. Until now, no reports have been available to describe the changes in cerebral oxygen metabolism following serious brain and during mild hypothermia treatment.OBJETCIVE: To observe the patterns of cerebral oxygen metabolism changes during mild hypothermia treatment for severe brain injury, and explore the mechanism of the therapeutic effect of mild hypothermia.DESIGN: A clinical observation of factorial design.SETTING: Mild Hypothermia Treatment Center of Tianjin Huanhu Hospital.PARTICIPANTS: From August 1998 to January 2000, 13 patients with severe brain injury were treated in Mild Hypothermia Treatment Center of Tianjin Huanhu Hospital, including 11 males and 2 females aged 18-65 years. Diagnosis of brain contusion and laceration with subdural hematoma was established in 6 cases, epidural hematoma in 1 case, subarachnoid hemorrhage in 4 cases and diffuse axonal injury in 2 cases. Of these cases 7 were treated with conservative therapy, and 6 with internal/external decompression after surgical hematoma removal.METHODS: A blanket for controlling the body temperature was applied to induce whole-body hypothermia in the patients in the mild hypothermia treatment room with continuous intravenous infusion of chlorpromazine (100 mg), promethazine (100 mg) and atracurium besilate (400 mg) administered in 500 mL normal saline. Neurotrend-7TM multi-parameter monitoring system was used to for monitoring the dynamic changes of cerebral PO2,PCO2, pH and brain temperature to evaluate their changes after treatment.The correlation between cerebral oxygen metabolism and the scores of Glasgow Coma Scale was analyzed.MAIN OUTCOME MEASURES: Dynamic changes of cerebral PO2,PCO2, pH and brain temperature.RESULTS: All the 13 patients entered the final analysis. Eighteen hours after hypothermia, the PO2 [(2.23±1.29) kPa] was obviously increased in comparison with that before hypothermia [(1.29±0.57) kPa, t=2.449, P ＜ 0.05], and PCO2 exhibited significant decrease at hypothermia 6 hours to (7.32±0.92) kPa from the pre-treatment level of (7.75±1.07) kPa (t=2.446, P ＜ 0.05). Significant elevation of pH and descension of intracranial pressure occurred upon the achievement of hypothermia [7.06±0.15 vs 6.83±0.20 for pH, t=5.164, P ＜ 0.05;(2.03±1.01) vs (2.57±0.93) kPa for intracranial pressure, t=2.948, P ＜ 0.05].Six hours after hypothermia, the cerebral perfusion pressure was obviously higher than that before hypothermia [(9.40±1.80) vs (7.80±1.59) kPa, t=2.365,P ＜ 0.05]. PCO2 was found inversely correlated with Glasgow Outcome Scale (GOS) scores at 24 hours of hypothermia (r=-0.699, P ＜ 0.05). The variations of cerebral oxygen metabolism indices before and after mild hypothermia were positively correlated with GOS scores.CONCLUSION: Dynamic monitoring of cerebral oxygen metabolism is safe and effective, and may help in early detection of cerebral hypoxia and acidosis following severe brain injury. Mild hypothermia treatment can effectively alleviate hypoxia and acidosis following severe brain injury to improve the prognosis of the patients.

To clarify the effects of zinc on the proliferation and apoptosis of cultured human retinal pigment epithelia (RPE) and the expression of caspase-3 in RPE cells. The effect of Zinc on the proliferation of RPE were examined with MTT method. TUNEL method was used to detect the apoptosis of RPE cells. Caspase-3 was detected by immunohistochemistry. A concentration of zinc higher than 0.001 microM could inhibit the proliferation of RPE. And the relationship between concentration of zinc higher than 10 microM and growth prohibition rate of RPE cells was dose-dependent. All concentrations of zinc including 0.001 microM enhanced the expression of caspase-3 of RPE. But only the concentration of zinc higher than 0.01 microM could induce apoptosis of RPE. It is concluded that zinc could enhance the expression of caspase-3 of RPE cells and induce apoptosis of RPE cells. Caution should be taken when using zinc supplements for the treatment of ARMD patients without deficiency of zinc.
Apoptosis/*drug effects ; Caspase 3 ; Caspases/metabolism ; Cell Division/drug effects ; Cells, Cultured ; Dose-Response Relationship, Drug ; Pigment Epithelium of Eye/*cytology ; Zinc/*pharmacology

Objective To evaluate the efficacy of the soft-shell technique with domestic materials during the cataract phacoemulsification. Methods Eighty-two eyes of 76 patients with the mature cataract phacoemulsification were divided into 3 groups randomly, including 28 eyes using the soft-shell technique with sodium tvlose and sodium hyaluronate (the domestic group),and 28 eyes using DuoVisc (Viscoat+ProVisc) (the import group), and 26 eyes using sodium hyaluronate (the common group). Results The average naked vision was 0.50 ± 0.22,0.51 ± 0.27,0.27 ± 0.21 respectively on the first day after operation.There was no signiticant difference between the domestic group and the import group (P＞0.05 ), while the first two groups and the common group had significant difference (P ＜ 0.05). The average naked vision was 0.61 ± 0.17,0.63 ± 0.18,0.58 ± 0.18 respectively 7 days after operation. There was no statistical difference among the three groups (P ＞ 0.05 ). The rate of corneal endothelial cells 1 month after operation was 7.1% in the domestic group,7.0% in the import group and 15.9% in the common group. It was clear that the rate in the domestic group and the import group was much lower than that in the common group. Meanwhile, the difference existed statistically between the two groups and the common group (P ＜ 0.01 ). However, the difference between the domestic group and the import group had no statistical significance. Conclusion The soft-shell technique with domestic materials is as safe and effective as that with the import materials in protecting the corneal endothelial cells during the cataract surgery in patients with the mature cataract.

Objective To explore the values of combined detection of serumhuman epididymis protein E4 , CA125 and CA199 in diagnosis of endometrial cancer. Methods 118 patients with endometrial cancer were chosen as group A, 82 patients with benign uterine disease were assigned to group B. Serum levels of HE4, CA125 and CA199 were detected and the positive rates were compared between the two groups. Results Serum levels of HE4, CA125, and CA199, the positive rate were significantly higher in group A than in group B (P <0.05). HE4 positive rate was highest in group A, and group B had no HE4-positive patients. The sensitivity and accuracy of HE4 + CA125 + CA199 were the highest, which was 94.9% and 92.5%. Levels of HE4, CA125 and CA199 and positive rate showed a rising trend as FIGO stage increased (P < 0.05), and HE4 positive rate was significantly higher than those of CA125 and CA199 (P < 0.05). Conclusions HE4 can be used to identify benign and malignant diseases in the uterus , and has a higher diagnostic value of endometrial cancer as compared with CA125 and CA199. Combined detection of HE4, CA125 and CA199 can improve the positive rate and diagnosis accuracy in endometrial cancer.

0.05) re spectively.Conclusion:E-Q system is rapid, simple and effective in root canal therapy.

To explore the velocity difference between specifications and categories of liquid with the same pressure infusion bag in order to widen the specification range of the liquid for pressure infusion. Pressure infu-sion experiments were performed with three specifications of normal saline of 100, 250 and 500 ml to compare the veloci-ties of different specifications of liquid, and with 500 ml normal saline, (5%, 10% and 50%) glucose injection, 5% glu-cose and sodium chloride injection, 5% sodium bicarbonate injection, 10% fructose injection, 706 plasma substitute, 20%mannitol injection and etc to make clear the velocities of different categories of liquid. With the same pressure, there were no significant differences between the velocities of three specifications of liquid, and between those of cate-gories of liquid with the same specification and concentration; the difference was significant between the same category of liquid with different concentrations, and the velocity showed a negative correlation with the concentration. The pressure infusion bag is compatible with 100, 250 and 500 ml liquid, and the velocity may be constant in case some specification of liquid is replaced by another one. The velocity has to be regulated in case the concentration or category of the liquid changes, when the pressure infusion is performed.

Deoxynupharidine is one of the alkaloids extracted trom Nuphar pumilum ( Timm ) DC which has been used in the treatment of arthritis and lumber-leg pain in folk medicine. This article presents the results of the experimental study of the analgesic and antiinflamma-tory actions of deoxynupharidine.Deoxynupharidine, ip, 15-20mg/kg, increased significantly the pain threshold of thermal and electrical stimulation in mice, and decreased significantly the rate and frequency of the development of body twisting reaction induced by ip acetic acid. Deoxynupharidine also significantly reduced exudation of acetic acid-induced inflammation in peritoneal cavity of mice. Data also showed that deoxynupharidine 15-20mg/kg and dexamethasone 4-8mg/kg produced a similar inhibing effeet on the degree of ankle swelling induced by subcutaneous injection of egg albumin. Dose-response relationship was seen in the above experiment.These resalts provide evidences for the clinial appli cation of deoxyuupharidine in the treatments of arthrtis and other inflammatory diseases.

Atrioventricular Block ; chemically induced ; Child ; Humans ; Male ; Mercury Poisoning ; complications

Chronic Disease ; Diagnosis, Differential ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Gastritis ; diagnosis ; drug therapy ; Humans ; Medicine, Chinese Traditional ; Phytotherapy ; Reference Standards