Adult ; Aged ; Female ; Humans ; Lymphoma, Follicular ; diagnosis ; Male ; Middle Aged ; Prognosis ; Young Adult

Objective To investigate the role of annexin A1 (ANXA1) in human pulmonary arterial smooth muscle cells (PASMCs) proliferation induced by hypoxia.Methods Primary cultured human PASMCs were randomly divided into 5 groups ( n =60 each):normal oxygen group (group N) and hypoxia 2,8,12 and 24 h groups(groups H1-4).Group N was treated with 21％ O2 for 24 h,and groups H1-4 was treated with 3％ O2 for 2,8,12 and 24 h respectively.ANXA1 mRNA and protein expression was determined by RT-PCR and Western blot.Proliferation of PASMCs were determined by MTT and 3 H-TdR methods.Results ANXA1 mRNA and protein expression was down-regulated,proliferation of PASMCs was enhanced in groups H1-4 as compared with group N (P ＜ 0.05).ANXA1 mRNA and protein expression was down-regulated gradually,proliferation of PASMCs was enhanced gradually in groups H1-4 ( P ＜ 0.05 ).Conclusion The expression of ANXA1 is down-regulated in human PASMCs during hypoxia treatment,which might be the mechanism of human PASMCs proliferation induced by hypoxia.

OBJECTIVETo introduce an arthroscopic technique in managing recurrent dislocation of the patella and its clinical results.

METHODSSixteen patients with recurrent patellar dislocation were reviewed, including 3 males and 13 females. The average age was 17.6 years old (ranged from 14 to 32 years). The patients suffering from patellar sub-luxation averaged 18.5 months (ranged from 6 to 23 months)before operation. These patients were treated with lateral patellar retinacular release outside the synovial bursa of knee joint and medial patellofemoral ligament reconstruction using the semitendinosus tendon free autograft. The Lyshohm scores before and after operation were used to evaluate outcomes at the final follow-up.

RESULTSAll the patients were followed up, and the duration ranged from 6 to 48 months, with an average of 12 months. There was no recurrence. The Q angle decreased from (16.4 ± 3.7)° to (10.1 ± 1.4)°; insall index decreased from 1.37 ± 0.25 to 1.28 ± 0.23; congruence angle decreased from (21.3 ± 2.6)° to (5.86 ± 2.23)°; Lysholm score improved from 76.1 ± 5.2 to 89.8 ± 4.1 at 6 months after operation.

CONCLUSIONCompared with conventional procedure, arthroscopic surgery for recurrent dislocation of the patella achieves excellent outcomes with minimum invasion.

Adolescent ; Adult ; Arthroscopy ; Bursa, Synovial ; surgery ; Female ; Humans ; Knee Joint ; surgery ; Male ; Patellar Dislocation ; physiopathology ; surgery ; Patellar Ligament ; surgery ; Range of Motion, Articular ; Treatment Outcome ; Young Adult

The type of basic media and the contents of plant growth substances were investigated by orthogonal design experiment,and also the effects of different culture conditions on the growth of suspension cells and the accumulation of total flavonoids in Eucommia ulmoides were studied.The results showed that B5 medium supplemented with 0.5mg/L NAA,0.6mg/L 6-BA and 30g/L sucrose,at initial pH 5.0～5.5,20g(FW)/L inoculation quantity and 110 r/min of rotation speed was a preferable culture conditions for E.ulmoides suspension cells growth and flavonoids synthesis.The results of metabolic kinetics analysis for E.ulmoides cell suspension culture showed that the logistic and Luedeking-Piret equations can be used for describing the kinetics of cell growth,sucrose consumption and flavonoids production during the process.The maximum specific growth rate(?m),the actual growth yield based on sucrose(YG) and maintenance coefficient(m) were 0.417/d,0.619g/g and 0.0206g/(g?d-1) respectively.All these outcomes could give a basis for establishing the suspension cell culture of E.ulmoides and production of the natural active components in large-scale.

Aim To study the influence of estradiol on peripheral blood stem cells and angiogenesis in a rat model of myocardial infarction.Methods Acute myocardial infarction(AMI)model was bulit by ligation of left anterior descending coronary in ovariectomized SD rats.Then,they were treated with different doses of estradiol benzoate.CD34+ cells in myocardium at 24 h after AMI were detected by immunohistochemistry,and CD34+ cells in peripheral blood were measured by flow cytometry on day 1,3 and 7 after AMI.The expression of stromal cell-derived factor-1(SDF-1)in myocardium on day 1,3 and 7 after AMI and capillary density in myocardium at 28 days after AMI were evaluated by immunohistochemistry.Results CD34+ cells in peripheral blood and myocardium,the expression of SDF-1 and capillary density at 4 weeks after AMI in ovariectomized rats were obviously lower than those in ovary intact rats.However,after indication with different doses of estrogen,CD34+ cells in peripheral blood and myocardium,the expression of SDF-1 and microvascular density were increased in ovariectomized rats with AMI,this effect was strongest especially in estrogen replacement therapy group.Conclusions Estrogen could improve the mobilization and homing of bone marrow stem cells into myocardium,increase capillary density and improve prognosis after AMI,and this mechanism could be mediated by promotion of expression of SDF-1 in myocardium.Moreover,the effect of estrogen replacement before AMI was better than estrogen therapy after AMI.

Objective　To explore the risk factors and causes affecting the operative mortality in esophagectomy patients with esophageal can cer. Methods　1400 cases with a curative esophagectomy for neopl asm of esophagus hospitalized from Mar,1973 to June, 2000 were reviewed. There w ere 31 died within 30 d or during hospitalization after esophagectomy as a group , and 1 369 survival cases, after operation, as another group. Sixteen factors t hat may influence the operational mortality were selected. A multi-variate anal ysis of these individual variables was performed by the computer′s logistic reg ression model. Results　The operative mortality was 2.2%(31/1400 ). The causes of death included respiratory complication 17 cases (including res piratory failure caused by pneumonia or atelectasis), 15 cases, and adult respir atory distress syndrome (ARDS) 2 cases, the mortality was 54.8% in the death gro up), anastomotic leak 11 cases (34.5%), Chylothorax 2 cases (6.5%) and postopera tive digestive tract hemorrhage 1 case (3.2%). The results showed that the major risk factors that affected operative mortality in cases with esophageal cancer were history of long-herm heavy smoking, duration of operation and the year of operational (P<0.05). Conclusion　To minimize operative mort ality of esophagectomy, some means must be noticed, including the reinforcemen t of the perioperative care, the improvement of anastomotic methods and surgical skill, reduing operative time as p ossible, disposing pulmonary complications in time and using respirator if neces sary.

OBJECTIVE: To seek a precise method for estimating postmortem interval (PMI) in forensic medicine practice. METHODS: This study detected the concentration of 21 elements in the vitreous humor of rabbit within 96 hours after death and explored the relationship between their concentration and PMI using the method of ICP-MS. RESULT: It was shown that the concentration of 9 elements in vitreous humor of rabbit within different intervals after death were correlated to PMI available as a reference indicator to estimate PMI. CONCLUSION The changes in the concentration of certain elements in vitreous humor of vitreous humor are correlated with PMI and determination of such changes can be used to estimate the time of death.
Animals ; Calcium/analysis* ; Forensic Medicine ; Iron/analysis* ; Magnesium/analysis* ; Postmortem Changes ; Potassium/analysis* ; Rabbits ; Time Factors ; Vitreous Body/chemistry*

Objective To summarize the auxiliary value of CT scanning in differentiating acute glioma stroke from simple cerebral hemorrhage. Methods The CT data of 12 patients with acute glioma stroke diagnosed by operation and pathological examination were analyzed retrospectively.Results CT scan presented high density hematoma with clear borderline in 12 cases, solid tumor mass in 9 cases, no clear tumor foci in 3 cases, and perifocal edema in 10 cases. Conclusion The combination with the clinical history, CT manifestations and enhanced CT scan can facilitate the diagnosis of acute glioma stroke from simple cerebral hemorrhage.

OBJECTIVETo evaluate the potential reconsideration of curative operative treatment for patients with unresectable stage IIIA (N2) non-small-cell lung cancer (NSCLC).

METHODSFrom Jan. 1999 to Dec. 2002, 76 patients with unresectable stage IIIA (N2) NSCLC were entered in this study. They had all been proved by chest CT, chest film and fiberobronchoscopy. Twenty-one (27.6%) patients were examined by mediastinoscopy. All the patients received two cycles of chemotherapy with NVB (25 mg/m(2), D1, D5) and carboplatin (300 mg/m(2), D1). All the patients were staged again three weeks after induction chemotherapy. Sixty-four patients who achieved partial response (PR) or complete response (CR) were allowed to undergo surgery. Twelve patients who did not responde to chemotherapy received radiotherapy instead. Of the 64 surgically treated patients, 56 (84.7%) had a complete resection and then received 2 cycles of chemotherapy using the same regime, 8 patients had an incomplete resection and then received radiotherapy for the residual tumor.

RESULTSThe median survival for these 76 patients with unresectable stage IIIA (N2) NSCLC treated by either surgery or radiation after induction chemotherapy was 18.6 months with 1-, 2-, 3-year survival rate of 64.2%, 39.4% and 25.6%, respectively. The median survival for the 56 patients with a complete resection was 28.2 months with 1-, 2-, 3-year survival rate of 70.4%, 52.5% and 38.6%, respectively.

CONCLUSIONPreoperative induction chemotherapy with NVB plus carboplatin should be seriously considered for the patients with unresectable stage IIIA (N2) NSCLC, It is suggested that, whenever possible, surgery should be taken as the first choice for the patients who show down-staged benefits that complete resection can be attempted.

Adult ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Carboplatin ; administration & dosage ; Carcinoma, Non-Small-Cell Lung ; drug therapy ; surgery ; Chemotherapy, Adjuvant ; methods ; Combined Modality Therapy ; Drug Administration Schedule ; Female ; Humans ; Lung Neoplasms ; drug therapy ; surgery ; Male ; Mediastinoscopy ; Middle Aged ; Preoperative Care ; Vinblastine ; administration & dosage ; analogs & derivatives

OBJECTIVETo evaluate variations of pulmonary venous drainage and venous ostium index (VOI) in patients with atrial fibrillation (AF) prior to radio-frequency catheter ablation (RFCA) by MDCT pulmonary venography.

METHODS16-detector row CT pulmonary venography was performed in 64 AF patients referred to RFCA from June, 2005 to May, 2006. Variations in pulmonary venous drainage were observed in volume render imagines. Anterior-posterior and superior-inferior diameters of pulmonary venous ostium were measured on maximum intensity projection images. VOI derived from left superior, left inferior, right superior, right inferior pulmonary veins and variations in pulmonary venous drainage were calculated.

RESULTSClassic pulmonary veins anatomy was found in 11 patients (17.18%), early branching veins in 45 patients (70.31%), left common ostium in 5 patients (7.81%), right common ostia in 1 patient, right accessory (middle) pulmonary vein in 5 patients (7.81%) and left accessory (middle) pulmonary vein in 1 patient (1.56%). VOI of homolateral pulmonary veins and bilateral superior pulmonary veins were similar (P > 0.05) while there was a significant difference on VOIs derived from left superior and right inferior; two inferior, left inferior versus right superior veins (P < 0.05). Right inferior pulmonary venous ostium was most rounded and had the highest index (0.88) and left inferior pulmonary venous ostium was most oval and had the lowest index (0.72).

CONCLUSIONMultidetector row CT pulmonary venography (MDCT-PV) could provide valuable informations on pulmonary venous anatomy in AF patients referred to RFCA and should be used as a routine examination prior to the operation.

Adolescent ; Adult ; Aged ; Atrial Fibrillation ; diagnostic imaging ; therapy ; Catheter Ablation ; methods ; Female ; Humans ; Male ; Middle Aged ; Pulmonary Veins ; abnormalities ; diagnostic imaging ; Tomography, Spiral Computed ; methods ; Young Adult