Face ; blood supply ; Hemangioma ; therapy ; Humans ; Vascular Malformations ; therapy ; Vasculitis ; therapy

Face ; blood supply ; Hemangioma ; classification ; diagnosis ; therapy ; Humans ; Jaw ; blood supply ; Lymphatic Abnormalities ; classification ; diagnosis ; therapy ; Mouth ; blood supply ; Vascular Malformations ; classification ; diagnosis ; therapy

Objective To investigate the association between serum N-terminal pro brain natriuretic peptide(NT-ProBNP) and creatine kinase myocardial(CK-MB),cardiac troponin I(cTnI) in children who were diagnosed as viral myocarditis(VMC),and to explore the clinical significance.Methods Fifty children with VMC were selected as VMC group who were further divided into cTnI(+)group and cTnI(-)group.Fifteen healthy children were selected as healthy control group.The levels of myocardial enzyme of the 2 groups were tested by automatic biochemical analyzer and level of NT-proBNP was detected by specific NT-proBNP enzymelink immunosorbent assay(ELISA) kit.SPSS 13.0 software was used to analyze the data.Results 1.There were 23 cases with positive cTnI and 27 cases with negative cTnI.The levels of NT-proBNP in VMC group including cTnI positive and cTnI negative were significantly higher than those in healthy control group (P0.05).2.In the group with positive cTnI,the level of NT-proBNP was correlated with cTnI.The coefficient of determination was 0.17.3.In the group with negative cTnI,the level of NT-proBNP was correlated with CK-MB.The coefficient of determination was 0.34.NT-proBNP presented a increasing trend during interval of 25-100 U?L-1 and 175-255 U?L-1 of CK-MB.Conclusions The levels in the serum NT-proBNP in VMC children were higher than those in healthy children.Serum detection NT-proBNP may be applied as a new approach in diagnosis of VMC in children.

Leydig cells are the major source of androgens in males. Stem cells can be induced to differentiate into androgen-secreting Leydig like cells, whose functions are regulated by the hypothalamus and pituitary, so that they precisely secret the necessary hormones to maintain physiological function. Therefore, the establishment of an effective protocol to induce the differentiation of stem cells into androgen-secreting cells is very helpful for the treatment of hypogonadism caused by abnormalities of Leydig cells. This review outlines the recent findings concerning the differentiation of stem cells into androgen-secreting cells.
Androgens ; secretion ; Cell Differentiation ; physiology ; Humans ; Hypogonadism ; therapy ; Hypothalamus ; physiology ; Male ; Pituitary Gland ; physiology ; Stem Cells ; cytology ; secretion

OBJECTIVEEndothelial apoptosis plays an important role in the initiation of atherosclerosis. It would be useful to clarify whether activation of non-neuronal muscarinic receptor (NNMR) could prevent endothelial apoptosis and atherosclerosis. We investigated the effects of NNMR activation on regulating rat aortic endothelial cells (RAECs) apoptosis induced by homocysteine, an independent risk factor of atherosclerosis, and further studied its molecular mechanism.

METHODSRAECs were incubated using homocysteine at the concentration of 2.7 mmol/L for 36 h. RAECs were also pre-treated with carbachol or arecoline to examine their effects. RT-PCR was used to assess changes in the gene expression related to cell apoptosis.

RESULTSIncubation of RAECs with homocysteine at the concentration of 2.7 mmol/L resulted in morphologic changes, such as cellular shrinkage, membrane blebbing, chromatin condensation and margination. These could be attenuated by pretreatment with carbachol and arecoline at the concentration of 10 micromol/L for 12 h. Homocysteine induced apoptosis in RAECs and the molecular mechanisms were associated with the regulation of fas, fas-L and caspase-8 in the death receptor pathway, bcl-2, bcl-xL and bax in the mitochondrial pathway, caspase-12 in the endoplasmic reticulum pathway and caspase-3, caspase-6 and p53 as downstream effectors. Carbachol and arecoline attenuated the effects of homocysteine on genes in the death receptor pathway, in the mitochondrial pathway and in the downstream pathway. Atropine could reverse all of the effects of arecoline.

CONCLUSIONActivation of NNMR by carbacol and arecoline inhibits homocysteine-induced endothelial cell apoptosis mainly through regulation of death receptor pathway, mitochondrial pathway and downstream effectors.

Animals ; Aorta ; cytology ; Apoptosis ; Apoptosis Regulatory Proteins ; metabolism ; Arecoline ; Carbachol ; Cell Cycle ; Endoplasmic Reticulum ; metabolism ; Endothelial Cells ; cytology ; drug effects ; Homocysteine ; adverse effects ; Mitochondria ; metabolism ; Rats ; Receptors, Muscarinic ; metabolism

Plasma exchange is a commonly used blood purification method, h includes plasma exchange,double filtration plasmapheresis and immunoadsorption.Many nervous system diseases have the antibody. Guillian-Barre syndrome is caused by antibodies of the nervous system demyelinafing disease; Myasthenia grads is caused by the acetylcholinc receptor antibodies. CIDP also has autoimmune-mediated mechanism. Plasma exchange can be apphed effectively to remove antibodies, and to quickly improve the patient' s clinical symptoms and shorten the course of disease.

Shan Qi soy has busting bags, bacteria sum overproof, continue acidification and other problems occasionally during the storage. It's probably caused by the Lactobacillus continue growth in the soy. We tried expounding the relationship between growth and producing acid when Lactobacillus growing in the thin fermented material.

Porcine Reproductive and Respiratory Syndrome virus (PRRSV) is the etiological agent of Por- cine Reproductive and Respiratory Syndrome. We summarized the recent research progress on molecular bi- ology of PRRSV including the structure of genome, viral structural and Non-structural protein.

Object To study comparatively the chromatographic fingerprint of Forsythia suspensa (Thunb ) Vahl in different producing aeras Methods Pyrolysis gas chromatography (PGC) was used Results The similar fingerprint of 13 samples (PGC) appeared The chromatographic overlap of them was over 89%, RSD