1.The technological innovation strategy for quality control of Chinese medicine based on Big Data.
Zhen-hao LI ; Zhong-zhi QIAN ; Yi-yu CHENG
China Journal of Chinese Materia Medica 2015;40(17):3374-3378
The evolution of the quality control concepts of medical products within the global context and the development of the quality control technology of Chinese medicine are briefly described. Aimed at the bottlenecks in the regulation and quality control of Chinese medicine, using Big Data technology to address the significant challenges in Chinese medicine industry is proposed. For quality standard refinements and internationalization of Chinese medicine, a technological innovation strategy encompassing its methodology, and the R&D direction of the subsequent core technology are also presented.
Data Mining
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Databases, Factual
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Drug Industry
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organization & administration
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standards
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Drugs, Chinese Herbal
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analysis
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pharmacology
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standards
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Humans
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Quality Control
2.Advance on chemical compounds of Ainsliaea genus.
Fa-jin FENG ; Zhi-ling XU ; Qian-jun ZHANG ; Zhen-hua YIN ; Wen-yi KANG
China Journal of Chinese Materia Medica 2015;40(7):1244-1251
Plants in Ainsliaea genus, belongs to Compositae family, are traditional Chinese medicine and widely used in folk. These plants contain various types of chemical components, and main components are sesquiterpene lactone and its glycosides. In addition, there are triterpenoids, flavonoids, steroids, phenolic acid, long chain fatty acid and volatile oils. Recently, much attention has been payed to varlous research of A. fragrans. This paper reviewed and summarized the chemical components to provide the theoretical basis for the use of Ainsliaea.
Asteraceae
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chemistry
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Drugs, Chinese Herbal
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chemistry
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pharmacology
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Molecular Structure
3.Study on lipid-lowering traditional Chinese medicines based on pharmacophore technology and patent retrieval.
Xiao-qian HUO ; Yu-su HE ; Lian-sheng QIAO ; Zhi-yi SUN ; Yan-ling ZHANG
China Journal of Chinese Materia Medica 2014;39(24):4839-4843
The combined application of statins that inhibit HMG-CoA reductase and fibrates that activate PPAR-α can produce a better lipid-lowering effect than the simple application, but with stronger adverse reactions at the same time. In the treatment of hyperlipidemia, the combined administration of TCMs and HMG-CoA reductase inhibitor in treating hyperlipidemia shows stable efficacy and less adverse reactions, and provides a new option for the combined application of drugs. In this article, the pharmacophore technology was used to search chemical components of TCMs, trace their source herbs, and determine the potential common TCMs that could activate PPAR-α. Because there is no hyperlipidemia-related medication reference in modern TCM classics, to ensure the high safety and efficacy of all selected TCMs, we selected TCMs that are proved to be combined with statins in the World Traditional/Natural Medicine Patent Database, analyzed corresponding drugs in pharmacophore results based on that, and finally obtained common TCMs that can be applied in PPAR-α and combined with statins. Specifically, the pharmacophore model was based on eight receptor-ligand complexes of PPAR-α. The Receptor-Ligand Pharmacophore Generation module in the DS program was used to build the model, optimize with the Screen Library module, and get the best sub-pharmacophore, which consisted of two hydrogen bond acceptor, three hydrophobic groups and 19 excluded volumes, with the identification effectiveness index value N of 2. 82 and the comprehensive evaluation index CAI value of 1. 84. The model was used to screen the TCMD database, hit 5,235 kinds of chemical components and 1 193 natural animals and plants, and finally determine 62 TCMs. Through patent retrieval, we found 38 TCMs; After comparing with the virtual screening results, we finally got seven TCMs.
Acyl Coenzyme A
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metabolism
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Animals
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Databases, Factual
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Drugs, Chinese Herbal
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chemistry
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pharmacology
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Hydrophobic and Hydrophilic Interactions
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Hydroxymethylglutaryl-CoA Reductase Inhibitors
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chemistry
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pharmacology
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Lipid Metabolism
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drug effects
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Lipids
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blood
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Medicine, Chinese Traditional
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Models, Molecular
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Quantitative Structure-Activity Relationship
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Technology
4.Application of early cleavage embryo in combination with embryo growth rate and morphology scoring in embryo selection
Yi-juan, SUN ; Ai-jun, ZHANG ; Xiao-wei, LU ; Zhi-hong, NIU ; Qian, CHEN ; Yun, FENG
Journal of Shanghai Jiaotong University(Medical Science) 2009;29(6):719-721
Objective To evaluate the role of early cleavage embryo in combination with embryo growth rate and morphology scoring in embryo selection in in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) cycles. Methods Six hundred and ten IVF/ICSI cycles were randomly assigned to group A(269 cycles) and group B(341 cycles). In group A, transferred embryos were chosen according to embryo growth rate and morphology scoring by 72 h(D3) after fertilization, while early cleavage embryo was added to the selecting system in group B. The pregnancy rate and implantation rate were compared between two groups, and the clinic outcomes were compared between transfers with early cleavage embryos and without early cleavage embryos in group B. Results The pregnancy rate and implantation rate in group B were significantly higher than those in group A (P < 0.05). Transfers with early cleavage embryos also achieved much higher pregnancy rate and implantation rate in group B (P < 0.01). Conclusion Compared with embryo growth rate and morphology scoring, early cleavage embryo in combination with embryo growth rate and morphology scoring can improve the clinical outcomes in IVF/ICSI cycles.
5.Endothelial progenitor cells homing to the orthotopic implanted liver tumor of nude mice.
Zhi, ZHU ; Gang, CHEN ; Xingrui, LI ; Qian, YIN ; Zhifang, YANG ; Jilin, YI
Journal of Huazhong University of Science and Technology (Medical Sciences) 2012;32(5):675-9
This study investigated the "homing" phenomenon in hepatocellular carcinoma (HCC). The "homing" specificity of endothelial progenitor cells (EPC) by establishing an orthotopic implantation model in nude mice. EPCs harvested from the marrow cells were separated by density gradient centrifugation. Fluorescence microscope, flow cytometry (FCM) and double fluorescence staining with FITC-UEA-I and DiI-ac-LDL, were employed to identify the cells. 4',6-diamidino-2-phenylindole (DAPI) labelling and real-time PCR were used for detecting the expression of CD133 and chemokines to trace and observe the distribution of EPCs. Our results showed that the distribution rate of EPCs was obviously higher than that in other important organs and the negative control group. Detection of CD133 and chemokines yielded similar results in difference tissues. Our experiment confirmed that the chemotaxis of EPCs does exist in HCC. Moreover, HIF-1α, SDF-1 and VEGF might play important roles in the "homing" of EPCs in HCC. EPCs might be a potential candidate for targeting vector of HCC for gene therapy.
6.Absorption of aqueous extracts from Salviae Miltiorrhizae Radix et Rhizoma by everted intestinal sac method.
Jie ZHAO ; Xue-lin XU ; Hong YI ; Hong-min ZHANG ; Xiao-qian LIU ; Jing-jing ZHU ; Zhi-min WANG
China Journal of Chinese Materia Medica 2015;40(15):3088-3093
To study the absorptive characteristics of aqueous extracts from Salviae Miltiorrhizae Radix et Rhizoma by in vitro rat everted intestinal sac model. Three representative ingredients in aqueous extracts from Salviae Miltiorrhizae Radix et Rhizome--protocatechuic aldehyde (PAL), posmarinic acid (RA) and salvianolic acid B (SAB), were selected as the study objects. An UPLC method was established to determine and measure their cumulative absorption amount, in order to explain the absorption characteristics of ingredients in different intestinal sections. According to the experimental result, RA and SAB showed the passive absorption in ileum, which conformed to the first-order absorption rate; with low and medium doses, they showed a zero-order absorption rate in jejunum, which was reflected in the coexistence of both positive and passive absorptions; PAL showed a passive absorption manner both in ileum and jejunum. According to the experiment for absorption in different intestinal sections, RA and SAB were mainly absorbed in jejunum, while PAL was absorbed mainly in ileum. All of the three ingredients in aqueous extracts from Salviae Miltiorrhizae Radix et rhizome--PAL, RA and SAB could be absorbed in intestines, but with differences in the absorption rate and mechanism, which indicated that the intestinal absorption of aqueous extracts from Salviae Miltiorrhizae Radix et rhizome was selectivity, instead of a simple semi-permeable membrane penetration process.
Animals
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Intestinal Absorption
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Male
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Plant Extracts
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pharmacokinetics
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Rats
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Rats, Sprague-Dawley
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Rhizome
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Salvia miltiorrhiza
7.Resilience in China recruits and its influencing factors
Yi MIAO ; Guojun FENG ; Min LI ; Qian CHU ; Li ZHI ; Yu GUO ; Jiajia ZHANG ; Ying XU ; Li PENG
Chinese Journal of Behavioral Medicine and Brain Science 2010;19(12):1105-1107
Objective To explore the characteristic and its influencing factors of resilience in Chinese recruits to provide the scientific evidence for the mental health training. Methods Resilience Scale for Adults (RSA) ,Self-report Symptom Checklist 90( SCL-90), Eysenck Personality Questionnaire(EPQ) and General SelfEfficacy Scale (GSES) were applied to 2459 recruits(2231 males and 139 females, aged ( 19 ± 1.5 ) ) within two male recruits( 101.76 ± 14.06) was significantly higher than that of males (96.65 ± 15.62) ( t=4. 13, P<0. 0l ) ;Recruits with College and above education have significantly higher RSA scores than those with junior high school education and senior high school education (P < 0. 0l ). Between the only child group and non only child group, there was no significant difference in RSA total score , but significant difference existed only in three factors higher RSA group scored significantly higher in extraversion (Z = 19.13, P = 0.000), abreaction adjustment (Z =8.67, P = 0.600 ) and self-efficacy (Z = 19.48, P = 0.000 ), while scored significantly lower in the SCL-90 (Z =Resilience had significant positive correlation with extraversion, abreaction adjustment and self-efficacy, and negative correlation with the neuroticism and inhibition adjustment. Multiple linear regression analysis revealed that extraversion, neuroticism,abreaction adjustment/inhibition adjustment and self-efficacy had predictive ability to resilience( explain 41% ). Conclusion Chinese recruits have good resilience. Emotion Regulation (abreaction adjustment/inhibition adjustment), extraversion, neuroticism and self-efficacy are important influencing factors of resilience for Chinese recruits.
8.Clinical application of thulium laser in thoracoscopic resection of pulmonary nodules
Yi ZHANG ; Ruotian WANG ; Kun QIAN ; Lei SU ; Lei LIU ; Mu HU ; Yuanbo LI ; Xin ZHAO ; Lin HUA ; Xiuyi ZHI
Chinese Journal of Thoracic and Cardiovascular Surgery 2017;33(6):360-362
Objective The aim of the present study was to evaluate the clinical application of 2 μm thulium laser in pulmonary nodules resection under VATS.Methods 61 patients,undergoing thoracoscopic resection of pulmonary nodules in thoracic department of Xuanwu Hospital,were identified between January and December of 2016.Of those,30 underwent 2μm thulium laser dissection and 31 were treated with standard technique by using staplers.In terms of clinical characteristics,including gender,age and smoking history,there is no significant difference between the laser group and the stapler group,but the lesion size was slightly larger in the stapler group compared with the laser group.The lesions of the two groups were almost evenly distributed on the five lobes.Results All the procedures were performed successfully under VATS.The intraoperative evaluation of air leaks demonstrated that less than or equal to 2 grade air leaks were observed in 28 cases in the laser group and in 30 cases in the stapler group.Grade 3 air leaks requiring a rescue treatment were observed in 2 cases in the laser group and in 1 case in the stapler group.There were no significant differences in the postoperative hospital stay and total hospital stay between two groups.Chest tube duration was lower in the laser group compared with the stapler group even if it was not statistically significant (2.71 vs 3.55 days).Hospitalization costs was significantly lower for the laser group.Conclusion The use of 2um thulium laser to prevent intra-and postoperative air leaks and bleeding is effective and makes patients recover quickly,which allows a minimally invasive,accurate and safe application during thoracoscopic resection of pulmonary nodules.
9.Satisfaction analysis on day-parting appointment for elderly hypertension contracted outpatients in community
Bo GAO ; Zhi-long QIAN ; Yi-hong SHEN ; Xing-lun LIANG
Shanghai Journal of Preventive Medicine 2020;32(5):430-
Objective To study the application effect of day-parting appointment for elderly hypertensive contracted outpatients in community. Methods In May 2018 two groups (experiment and control) of 103 elderly hypertensive contracted outpatients, aged between 60 and 80 and looked after by the team of family doctors, who had been diagnosed with hypertension and with medication for at least one year were set up.The experimental group used self-made community hypertension visiting card for appointments, and the control group used the original way of treatment.Six months later, comparison was made in blood pressure control, the number of outpatients, the time consumed and the satisfaction between the two groups. Results It was found in comparison that the blood pressure standard-reaching rate of the experimental group was better than that of the control group (
10.Effects of Tangweian granule on 5-HT(2A)R in rat model with diabetic gastroparesis.
Qiu-Hai QIAN ; Zhi-Cheng WANG ; Yi ZHAO ; Da-Wen LIU ; Wei-Bin QIAN
China Journal of Chinese Materia Medica 2008;33(5):541-544
OBJECTIVETo observe the effects of Tangweian granule on 5-HT(2A)R in rat model with diabetic gastroparesis (DGP).
METHODThe rats with diabetic gastroparesis induced by injecting alloxan and giving 200% Radix Rehmanniae preparata were divided into four groups randomly: Tangweian high dosage group, Tangweian low dosage group, motilium control group and the model control group, 10 rats each group. Each group was irrigated with drugs during establishing the model. Additionally, we chose 10 rats by way of normal control group. Further more, Tangweian high dosage group were irrigated stomach with gliclazide 20 mg x kg(-1) and Tangweian granule 31.75 g x kg(-1); Tangweian low dosage group were irrigated stomach with gliclazide 20 mg x kg(-1) and Tangweian granule 15.88 g x kg(-1); motilium control group were irrigated stomach with gliclazide 20 mg x kg(-1) and motilium 3.75 mg x kg(-1) and the model control group were irrigated stomach with distilled water. Then the effects of Tangweian granule on 5-HT(2A)R were observed.
RESULTThe curative group had better effects than the control group in lowering the blood sugar and the level of 5-HT(2A)R content (P < 0.01). And there was significant difference between the curative group and control group (P < 0.05).
CONCLUSIONIt is verified that Tangweian granule has obvious effects on lowering the blood sugar and improving the level of 5-HT(2A)R.
Animals ; Blood Glucose ; drug effects ; Diabetes Mellitus, Experimental ; complications ; metabolism ; Disease Models, Animal ; Drugs, Chinese Herbal ; pharmacology ; Gastroparesis ; drug therapy ; metabolism ; Gliclazide ; pharmacology ; Male ; Random Allocation ; Rats ; Rats, Wistar ; Receptor, Serotonin, 5-HT2A ; metabolism