2.Specific role of synovial macrophages in rheumatoid arthritis
YANG XUE-ZHI ; CHANG YAN ; WEI WEI
Chinese Journal of Pharmacology and Toxicology 2017;31(10):1026-1026
Rheumatoid arthritis (RA) is an autoimmune disease, which is characterized by synovial inflammation. Hyperplasia sublining macrophages found in synovium is an early hallmark of RA and effective treatment results in their diminution. However, the origin of these sublining macrophages in synovium (including infiltrated macrophages and tissue-resident macrophages) are still unknown both in animal models of arthritis and RA patients, let alone the differences and feature of these macro?phages. In rheumatic synovium, macrophages are submitted to a large variety of micro-environmental signals which influence the phenotypic polarization and activation of macrophages. Understanding the mechanisms and functional consequences of the heterogeneous macrophages will contribute to confirm their potential role in synovial inflammation development. Furthermore, research on macrophage plasticity to soft-control their phenotypic polarization could lead to novel therapeutic approaches.
3.Analysis of influencing factors on adverse reaction of Shengfu injection based on prospective active safety monitoring.
Zhi-fei WANG ; Wei ZHAO ; Yin ZHANG ; Yan-ming XIE
China Journal of Chinese Materia Medica 2015;40(24):4746-4751
This study collected demographic data, past history, personal history, family history, dosage, solvent, combined medication information of adverse reaction cases from a prospective, multi center, large sample intensive hospital monitoring, and found the influencing factors with cross-tab analysis. The results showed that in the medication group of 19-45, patients with allergic histories had a higher proportion in ADR; in the medication group of 46-65, male patients with allergic histories had a higher proportion in ADR; indication and non-indication group, patients of 19-45 years old, with combined medications and allergy histories had a higher proportion in ADR; Non-indication medication group, patients with combined medication, higher concentration, out-instruction solvent and dosage, had a higher proportion in ADR. So, the ADRs of Shenfu injection were related to the history of drug allergy, and also related to the indication, dosage, solvent, concentration when it was used.
Adult
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Aged
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Drug Hypersensitivity
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etiology
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Drugs, Chinese Herbal
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adverse effects
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Female
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Humans
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Injections
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Male
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Middle Aged
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Prospective Studies
4.Treatment of 7 patients with medial meniscal cyst under arthroscopy.
Yan-guo HE ; Min WEI ; Zhi-qiang ZHANG ; Chao CHEN
China Journal of Orthopaedics and Traumatology 2014;27(8):642-644
OBJECTIVETo investigate clinical effects of arthroscopy in the treatment of medial meniscal cyst.
METHODSFrom June 2011 to January 2013, 7 patients with medial meniscal cyst were treated with arthroscopy. There were 3 males and 4 females,ranging in age from 27 to 63 years old,with a mean age of (43.93±2.10) years old. The cysts have been discovered for 3 to 30 months,with a mean time of (10.6±1.3) months. All the patients complained of knee pain,especially in the medial joint gap. The Pisani sign, Caklin sign and medial McMurray sign were all positive. Preoperative MRI examination confirmed the diagnosis. Lysholm score changes and clinical efficacy were observed through a six-month follow-up.
RESULTSThe postoperative Lysholm scores were all significantly higher than the preoperative scores. According to Sarimo standard, 6 patients got an excellent result, and 1 good.
CONCLUSIONArthroscopic treatment of medial meniscal cyst has replaced the traditional method, which could retain the normal meniscus as much as possible and repair the meniscus injury simultaneously, as well as get a good curative effect and a good recovery of knee function. This method is worthy of clinical application.
Adult ; Arthroscopy ; methods ; Cysts ; physiopathology ; surgery ; Female ; Humans ; Male ; Menisci, Tibial ; physiopathology ; surgery ; Middle Aged
6.Comparative study of main components of ginseng on immune function of rats.
Zhi-Ying JIA ; Xie XIE ; Xiao-Yan WANG ; Wei JIA
China Journal of Chinese Materia Medica 2014;39(17):3363-3366
Ginseng and its effective components are famous for their influence to enhance human immunity, regulate endocrine and antioxidant action. However, the different effects of different components are not clear. In this study, Wistar rats were used to study the effects of main components of ginseng, including total ginsenoside, panaxadiol saponins, panaxtrol saponin and ginseng polysaccharide. The results showed that the effects of panaxadiol saponins and ginseng polysaccharide on improving animal immune organ weight, plasma interleukin 2 (IL-2), interleukin 6 (IL-6), plasma gamma-interferon (IFN-γ), tumor necrosis factor alpha (TNF-α) were better than that of the other groups. Total ginsenoside and panaxtrol saponin can effectively increase the concentration of spleen NK cells (NKC) while panaxadiol saponins and ginseng polysaccharide can significantly increase the concentrations of rat plasma adrenocorticotrophic hormone (ACTH), corticosterone (CORT) and thyroid stimulating hormone (TSH). As for the effect of increasing organization nitric oxide (NO) and superoxide dismutase (SOD), glutathione (GSH) and malondialdehyde (MDA), total ginsenoside is better than that of other groups. In brief, different components in ginseng possess different effects on enhancing immunity, regulating endocrine and resisting oxidation. Panaxadiol saponins and ginseng polysaccharide are better in enhancing immune, and total ginsenoside shows advantages in resisting oxidation and stress.
Adrenal Glands
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drug effects
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growth & development
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Adrenocorticotropic Hormone
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blood
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Animals
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Brain
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drug effects
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metabolism
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Corticosterone
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blood
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Ginsenosides
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pharmacology
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Glutathione
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metabolism
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Immune System
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drug effects
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physiology
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Interferon-gamma
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blood
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Interleukin-2
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blood
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Interleukin-6
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blood
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Killer Cells, Natural
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drug effects
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Malondialdehyde
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metabolism
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Nitric Oxide
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metabolism
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Organ Size
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drug effects
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Panax
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chemistry
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Polysaccharides
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pharmacology
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Random Allocation
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Rats, Wistar
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Saponins
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pharmacology
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Spleen
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drug effects
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growth & development
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Superoxide Dismutase
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metabolism
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Thymus Gland
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drug effects
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growth & development
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Thyrotropin
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blood
7.Mangiferin protects rats against chronic bronchitis via regulating NF-kappaB (P65) and IkappaBalpha expression in mononuclear cells.
Zhi-Quan WEI ; Li YAN ; Jia-Gang DENG ; Jing DENG
Acta Pharmaceutica Sinica 2014;49(5):596-601
This study is to investigate the protective effect of mangiferin on NF-kappaB (P65) and IkappaBalpha expression in peripheral blood mononuclear cell (PBMC) in rats with cigarette smoke induced chronic bronchitis. The rat model with chronic bronchitis was established by cigarette smoke. Real-time fluorescence RT-PCR was executed for evaluating the NF-kappaB (P65) and IKkappaBalpha gene expression in mononuclear cell, and flow cytometry for their protein expression. The serum hs-CRP (high-sensitivity C-reactive proteins) and TNF-alpha (tumor necrosis factor-alpha) were detected by enzyme-linked immunosorbent assay. The histopathological score was obtained from lung tissue HE staining slides of lung tissue. The results showed that mangiferin could markedly suppress the NF-kappaB (P65) mRNA and protein expression in mononuclear cell, while promote the IkappaBalpha mRNA and protein expression. Furthermore, mangiferin could lower serum hs-CRP and TNF-alpha level, and reduce the chronic inflammatory damage of bronchiole. These results suggested that mangiferin could notably ameliorate chronic bronchiole inflammation induced by cigarette smoke, and this protective effect might be linked to the regulation of NF-kappaB (P65) and IkappaBalpha expression in mononuclear cell.
Animals
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Bronchi
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pathology
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Bronchitis, Chronic
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blood
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etiology
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metabolism
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pathology
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C-Reactive Protein
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metabolism
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I-kappa B Kinase
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genetics
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metabolism
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Leukocytes, Mononuclear
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metabolism
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pathology
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Male
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Mangifera
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chemistry
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Plants, Medicinal
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chemistry
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RNA, Messenger
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metabolism
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Random Allocation
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Rats
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Rats, Sprague-Dawley
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Tobacco Smoke Pollution
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Transcription Factor RelA
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genetics
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metabolism
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Tumor Necrosis Factor-alpha
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blood
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Xanthones
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isolation & purification
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pharmacology
8.Feasibility of different xenogeneic (porcine) acellular dermal matrices in the repair of deep burn wounds:a 12-month follow-up
Ming ZENG ; Dinan WEI ; Yan ZHI ; Hong WANG ; Zonghua CHEN
Chinese Journal of Tissue Engineering Research 2016;20(11):1551-1557
BACKGROUND:Skin grafting is crucial for patients with deep burns, but limited source of autologous skin grafts is an existing difficulty.
OBJECTIVE:To investigate the effect of xenogeneic (porcine) acelular dermal matrix in the treatment of deep burn wounds and the feasibility of its application.
METHODS:Forty-one patients with deep burn were divided into two groups according to the intention of the patients, 21 cases in control group and 20 cases in observational group, folowed by autologous split-thickness skin grafting alone or combined with different (porcine) acelular dermal matrix, respectively. After 12 months of folow-up, the graft success rate at postoperative 1, 2, 3, 4 weeks and skin graft contraction rate and wound repair at postoperative 3, 6, 9, 12 months were observed and compared between two groups. Moreover, levels of inflammatory factors were detected and compared between two groups at postoperative 1, 2, 3 months.
RESULTS AND CONCLUSION: The skin graft success rates showed no difference between two groups at postoperative 1, 2, 3, 4 weeks (P > 0.05). The skin graft contraction rates also showed no difference between two groups at postoperative 3, 6, 9, 12 months (P > 0.05). After 12 months of folow-up, no serious scar hyperplasia, but soft texture appeared in the control group. In the observational group, three cases presented with local pigmentation at the early stage, but it gradualy subsided with time; no obvious scar, but only smal, point-like scar, was visible, and the repaired wound exhibited soft touch. No adverse events and death occurred in both two groups. Experimental results show that the treatment of deep burns with autologous split-thickness skin grafting combined with xenogeneic (porcine) acelular dermal matrix is safe and effective, which can improve the quality of wound healing.
9.The Toxic Effects of the Extracellular Active Components from One Algae-lysing Bacteria on Chlorella Pyrenoidosa
Chun-Min ZHANG ; Wei-Bin PAN ; Yan-Zhi CHEN ;
Microbiology 1992;0(06):-
Toxic effects of L7 lyophilized powder of extracellular active components (L7-LPEAC), extracted from the Algae-lysing bacteria L7, on Chlorella pyrenoidosa were studied according to the changes of effective photosynthesis rate (EPR), the protein content, the chlorophyll a content and the MDA content of algae. The results showed that the growth of alga was promoted at low concentrations of L7-LPEAC (0.80 g/L, 1.25 g/L). The 96 h-EC50 and 120 h-EC50 upon Chlorella pyrenoidosa are 5.75 g/L and 2.55 g/L, respec tively. The chlorophyll a content increased firstly and then decreased at high concentrations of L7-LPEAC (≥2 g/L), so did the protein content. Compared with the control group, there is a significant statistics diff erence (P
10.Curcumin inhibits mouse melanoma growth and influences nuclear factor?B activation and sur-vivin expression
Yan-Qun LIU ; Lin ZHOU ; Zhi-Ping WEI ;
Chinese Journal of Dermatology 2003;0(11):-
Objective To investigate the possible mechanism of curcumin inhibition to murine melanoma growth.Methods Melanoma cell line B16F10 was injected subcutaneously into the outer side of mouse right thigh to establish a melanoma-bearing mouse model.Seven days after the establishing the model, these mice were treated with intraperitoneal injection of curcumin at 50 and 100 mg/kg respectively or RPMI 1640 culture medium as control.Fourteen days later,the mice were killed,tumor weight was calculated;the tumor nuclear factor?B activity and survivin mRNA expression were measured by Western blot and RT-PCR,respectively.Results The tumor weight was significantly lower in the curcumin-treated mice than that in the controls (P