2. Interaction of Hsc73 with CXCR4 in nuclei of renal cell carcinoma A498 cells
Academic Journal of Second Military Medical University 2010;31(7):702-705
Objective: To investigate the role of protein Hsc73 (heat shock cognate protein 73 000) in renal cell cancer metastasis promoted by SDF-1/CXCR4 axis, so as to determine whether Hsc73 participates in CXCR4 nuclear localization. Methods: Western blotting analysis was used to observe the expression of Hsc73 in A498 cells over-expressing CXCR4. The location of Hsc73 and the interaction of CXCR4 with Hsc73 were investigated in SDF-1-stimulated A498 cells by immunohistochemical staining, Co-IP (Co-Immunoprecipitation) experiment, etc. Results: Hsc73 was up-regulated in A498 cells over-expressing CXCR4. Hsc73 was mainly found in the cytoplasm of A498 cells; after stimulation with SDF-1, some Hsc73 appeared in the nuclei. Hsc73 protein was found in the nuclei of A498 cells after Co-IP with anti-CXCR4 antibody. Conclusion: Hsc73 as a common molecular chaperone participates in the intra-cellular translocation of CXCR4; Hsc73 also plays a key role in the activation of SDF-1/CXCR4 signal pathway and may be involved in the nuclear translocalization of CXCR4.
3.Not Available.
Journal of Forensic Medicine 2021;37(5):736-738
4.Analysis of complications after ultrasound-guided PTBD
Xiang JING ; Zhi DU ; Yijun WANG ; Jianmin DING ; Yandong WANG
Chinese Journal of Hepatobiliary Surgery 2010;16(8):600-603
Objective To investigate the reasons of complications after ultrasound-guided PTBD and explore measures for their management. Methods The clinical data of 753 patients with obstructive jaundice treated with ultrasound-guided PTBD in our hospital from January 2001 to June 2008were retrospectively analyzed. Results The 753 patients received a total of 791 treatments of PTBD.The occurring rates of severe, common, and long-term complications were 1.14 % (9/791), 11.88 %(94/791 ) and 4.3% (9/791), respectively. Only 1 patient died of manipulation-related complications.Conclusion Ultrasound-guided PTBD is an effective method for treatment of obstructive jaundice.
5.Three-dimensional reconstruction of brain surface anatomy:technique comparison between flash and diffusion-weighted imaging
Jian-Zhong SUN ; Zhi-Kang WANG ; Xiang-Yang GONG ;
Chinese Journal of Radiology 2001;0(08):-
Objective To compare two methods 3D flash and diffusion-weighted images(DWI)in reconstructing the brain surface anatomy,and to evaluate their displaying ability,advantages,limitations and clinical application.Methods Thrity normal cases were prospectively examined with 3 D flash sequence and echo-planar DWI.Three-dimensional images were acquired with volume-rendering on workstation.Brain surface structures were evaluated and scored by a group of doctors.Results Main structures of brain surface were clearly displayed on three-dimensional images based on 3D flash sequence.Average scores were all above 2.50.For images based on DWI,precentral gyrus,postcentral gyrus,superior parietal lobule, superior frontal gyrus,precentral sulcus,central sulcus,postcentral sulcus,intraparietal sulcus and superior frontal sulcus were best shown with average scores between 2.60-2.75,However,supramarginal gyrus, angular gyurs,middle frontal gyrus,inferior frontal gyrus,superior temporal gyrus,lateral sulcus,inferior frontal sulcus could not be well shown,with average scores between 1.67-2.48.Middle temporal gyrus, inferior temporal gyrus,superior temporal sulcus and inferior temporal sulcus can only get scores from 0.88 to 1.27.Scores of images based on 3D flash were much higher than that based on DWI with distinct differentiations,P values were all below 0.01.Conclusion Three-dimensional images based on 3D flash can really display brain surface structures.It is very useful for anatomic researches.Three-dimensional reconstruction of brain surface based on DWI is a worthy technique to display brain surface anatomy, especially for frontal and parietal structures.
6.Application of patent ductus arteriosus occluder in transcatheter occlusion of coronary artery fistula
Yunbin XIAO ; Zhi CHEN ; Xiyong HUANG ; Xiang WANG ; Zhou YANF
Journal of Interventional Radiology 2014;23(10):853-856
Objective To assess the clinical short-term to mid-term efficacy of transcatheter closure of coronary artery fistula by using patent ductus arteriosus occluder in pediatric patients. Methods During the period from Jan. 2008 to May 2013 at authors’ hospital, transcatheter closure of coronary artery fistula using patent ductus arteriosus occluder was performed in 8 pediatric patients. The clinical data, including follow-up information, were retrospectively analyzed. Results A total of 8 pediatric patients with a mean age of (4.1 ± 3.8) years were enrolled in this study. The fistula originated from the right coronary artery in five cases and from the left coronary artery in three cases. The blood flow shunted to the right atrium (n=4) or to the right ventricle (n = 4). Obstruction of the fistula was successfully accomplished in all patients. All patients were followed up for (2.2 ± 1.2) years. No procedure-related complications or cardiac ischemia occurred. Conclusion For the treatment of coronary artery fistula in pediatric patients, the use of domestic patent ductus arteriosus occluder is safe and effective with satisfactory short-term to mid-term clinical efficacy.
7.Recovery prediction of danshensu,salvianolic acid B,and salvianolic acid D in danshen ethanol precipitation
Xiang YU ; Biqi MO ; Zhi WANG ; Haibin QU
Chinese Traditional and Herbal Drugs 1994;0(06):-
Objective To develop recovery prediction models for danshensu,salvianolic acid B,and salvianolic acid D in danshen ethanol precipitation.Methods Recorded the operation parameters of 15 normal batches of danshen ethanol precipitation in one Chinese medicine plant and measured five active component contents in danshen concentrated liquid and danshen ethanol precipitated liquid,the Stepwise-MLR recovery prediction models of dashensu,salvianolic acid B,and salvianolic acid D were developed based on an approach which combined the process parameters and the active component contents in danshen concentrated liquid.The importance of variables in the prediction models was analyzed later.Results The coefficient correlations of dashensu,salvianolic acid B,and salvianolic acid D recovery prediction models were all above 0.95.Conclusion The models developed for active component recovery prediction show a good predictability.Models can be used to help manufacturers to understand the ethanol precipitation process and improve the quality control ability of danshen ethanol precipitation.
8.Therapeutic Effect of High Frequency Oscillatory Ventilation on Premature Infants with Respiratory Distress Syndrome
yan, WANG ; xiang-yong, KONG ; zhi-chun, FENG
Journal of Applied Clinical Pediatrics 2006;0(14):-
Objective To evaluate the effect of the high frequency oscillatory ventilation(HFOV) on the treatment of premature neonates with respiratory distress syndrome(RDS).Methods Seventy premature infants with RDS were randomly grouped as HFOV group(n=33) and conventional mandatory ventilation group(CMV group,n=37),based on their fetal age,weight,age,and their clinical condition from Jan.to Sep.in 2009.The blood gas analysis was detected and compared between the 2 groups.Results In HFOV group,the inhaled oxygen concentration,pa(CO2) decreased after treatment for 6 h,which were significantly lower than those at the beginning of the therapy(Pa
9.Effects of selective Cox-2 inhibitor in preventing postoperative recurrence of colorectal cancer
zhi-ming, JIN ; tian-xiang, WANG ; lu, YIN
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(04):-
0.05),but the 3-year recurrent rates were significantly different(P
10.Role of NO Pathway in Membrane Estrogen Receptor Mediated Proliferation and Apoptosis of Endothelial Progenitor Cells
Zhi TAN ; Yuhong CUI ; Qiuling XIANG ; Guiping LIN ; Tinghuai WANG
Journal of Sun Yat-sen University(Medical Sciences) 2010;31(1):64-68
[Objective] The aim of the present study was to investigate the role of membrane estrogen receptor (mER) mediated pathway in the proliferation and apoptosis of endothelial progenitor cells (EPCs). [Methods] Bone marrow (BM)-derived EPCs were cultured. The cells were divided into different groups, plus or not plus estrogen receptor blocker (ICI 182,780), PI3K inhibitors (LY294002), and NOS inhibitor (L-NAME) to show the effect of E_2-BSA on EPCs. The proliferation of EPCs was determined by MTT and nitric oxide (NO) release was measured by chromatometry. Apoptotic cell death was determined using the Hochest 33258 staining. The expression of phosphorylated eNOS (p-eNOS) were detected by Western blot. [Results] E_2-BSA could increase EPCs proliferation, and this effect was inhibited by estrogen receptor blocker ICI 182,780, thus indicated that mER-initiated membrane signaling pathways were involved in the action of estrogen on EPCs. E_2-BSA increased nitric oxide production and inhibited apoptosis induced by serum withdrawal, and this effect also inhibited by PI3K inhibitor (LY294002), NOS inhibitor (L-NAME)and estrogen receptor blocker(ICI 182,780), thus indicated that PI3K/Akt/NO pathway was involved the effect of estrogen on EPCs apoptosis. Moreover, E_2-BSA treatment increased phosphorylation of eNOS (p-eNOS). PI3K inhibitors (LY294002) also blocked these effects. [Conclusions] The results of present study suggested that mER mediated EPCs proliferation and apoptosis were related to the PI3K/Akt/eNOS pathway.