Antibodies, Monoclonal, Murine-Derived ; metabolism ; Diagnosis, Differential ; Female ; Follow-Up Studies ; Hemangioendothelioma ; metabolism ; pathology ; surgery ; Hemangioendothelioma, Epithelioid ; metabolism ; pathology ; surgery ; Humans ; Lymphatic Metastasis ; Middle Aged ; Platelet Endothelial Cell Adhesion Molecule-1 ; metabolism ; Sarcoma ; metabolism ; pathology ; surgery ; Thigh ; Vimentin ; metabolism ; von Willebrand Factor ; metabolism

In recent years, the studies on moxibustion for treatment of cancer are increasing day by day, with some advances. In the paper, clinical and experimental studies on traditional moxibustion for treatment of cancer and the mechanisms are reviewed, and some problems and shortcomings are put forward.
Acupuncture Therapy ; Humans ; Moxibustion ; Neoplasms

OBJECTIVETo set up a new grading system of intraventricular hemorrhage (IVH) and determine the value of predicting the probability of post-hemorrhagic hydrocephalus (PHH) in IVH.

METHODSWe first modified the Graeb criteria, then compared the value of prediction for PHH assessed by the Graeb criteria with the modified Graeb criteria. One hundred and thirty one IVH patients were divided into two groups: the upper group (n = 67) and the lower group (n = 64). Gold standard of PHH was assessed by CT scan or by out-drainage. The diagnostic parameters such as sensitivity (SE), specificity (SP) were analyzed. In the cutoff point of SE and SP curves, diagnostic efficiency (DE), and Kappa value (K) were analyzed. The probability of PHH was estimated by binary logistic regressions.

RESULTSIn all ventricular group, to Graeb criteria in the cutoff point, SE, SP, and K was 0.78, 0.84, and 0.60; and to modified Graeb criteria SE, SP, and K was 0.90, 0.84, and 0.74 respectively. The probability of PHH from point of 3-12 was 0.011, 0.032, 0.085, 0.212, 0.435, 0.689, 0.865, 0.949, 0.981, and 0.994 respectively according to modified Graeb criteria.

Aged ; Cerebral Hemorrhage ; complications ; diagnostic imaging ; Female ; Humans ; Hydrocephalus ; diagnosis ; diagnostic imaging ; etiology ; Logistic Models ; Male ; Middle Aged ; Sensitivity and Specificity ; Tomography, X-Ray Computed

Adult ; Humans ; Lateral Ventricles ; pathology ; Male ; Neurocytoma ; pathology

Adult ; Brain ; pathology ; Brain Neoplasms ; metabolism ; pathology ; surgery ; Diagnosis, Differential ; Female ; Ganglioglioma ; metabolism ; pathology ; surgery ; Humans ; Neoplasms, Neuroepithelial ; pathology ; S100 Proteins ; metabolism ; Synaptophysin ; metabolism

Adenocarcinoma ; metabolism ; pathology ; Adult ; Antigens, CD34 ; metabolism ; Biopsy ; Diagnosis, Differential ; Follow-Up Studies ; Hemangioendothelioma, Epithelioid ; diagnostic imaging ; metabolism ; pathology ; surgery ; Humans ; Lung ; metabolism ; pathology ; Lung Neoplasms ; diagnostic imaging ; metabolism ; pathology ; surgery ; Male ; Mesothelioma ; metabolism ; pathology ; Middle Aged ; Multimodal Imaging ; Platelet Endothelial Cell Adhesion Molecule-1 ; metabolism ; Positron-Emission Tomography ; Thoracoscopy ; Tomography, X-Ray Computed ; Tuberculosis, Pulmonary ; metabolism ; pathology

Objective To evaluate the possibility of methylation analysis of secreted frizzled-related protein gene 2 (SFRP2),hyperplastic polyposis protein gene (HPP1) and O~6-methylguanine-DNA methyhransferase gene (MGMT) in feces for screening colorectal cancer (CRC).Methods Total DNA was isolated from fecal samples of 52 patients with colorectal cancer and 35 patients with benign colorectal diseases and 24 normal volunteers,and the methylation status of SFRP2,HPP1 and MGMT was analyzed with methylation-specific polymerase chain reaction.Results SFRP2,HPP1 and MGMT were methylated in 94.2%,71.2%,48.1% of CRCs,respectively.At least one of the three genes was methylated in 96.2% of CRCs and 81.8% of precancerous lesions,respectively.In contrast,of the 24 normal controls,only one had methylated SFRP2.These results indicated 93.7% sensitivity,77.1% specificity,84.3% positive predicative value and 90.2% negative predictive value of the test for detecting CRC and precancerous lesions.Conclusions Methylation analysis of SFRP2,HPP1 and MGMT gene in stool is a high sensitive screening tool for CRC and precancerous lesions.Analysis of fecal DNA methylation is expected to serve as a new detection way for CRC or a new screening tool for individuals at risk of developing colorectal neoplasm.

Adult ; Aged ; Biomarkers, Tumor ; metabolism ; Carcinoma, Basal Cell ; metabolism ; pathology ; surgery ; Humans ; Keratin-5 ; metabolism ; Keratins ; metabolism ; Male ; Membrane Proteins ; metabolism ; Neoplasm Recurrence, Local ; Prostate ; metabolism ; pathology ; surgery ; Prostatectomy ; Prostatic Neoplasms ; metabolism ; pathology ; surgery

OBJECTIVETo observe the expression of homeobox gene Msx-1, Msx-2 and Dlx-2 during murine mandibular first molar development.

METHODSThe murine heads or mandibles on embryonic days 11-18 (E11-18) and postnatal day 1-3 (P1-3) were removed, fixed and embedded, 5 micro m serial sections were cut in the coronal plane. Msx-1, Msx-2 and Dlx-2 RNA probes were synthesized by in vitro transcription and labeled with digoxigenin. Msx-1, Msx-2 and Dlx-2 mRNA expression was observed after in situ hybridization.

RESULTSDuring molar development Msx-1 transcripts appeared only in mesenchymal cells, not in epithelial cells. Msx-2 and Dlx-2 both expressed in the epithelial and mesenchymal cells. At the initiation stage of the molar development Msx-2 and Dlx-2 had similar expression. At the bud stage (E13-14) Msx-2 mRNA signaling was intensive in the enamel organ and slight in the dental mesenchyme; Dlx-2 signaling was stronger in the dental papilla. At cap stage (E15-16) Msx-2 showed prominent mRNA signaling in enamel knot and Dlx-2 was maximal in the dental papilla. At the late bell stage (P2-3) Msx-2 transcripts were observed in odontoblasts but not labeled in ameloblasts, and Dlx-2 transcripts appeared in ameloblasts but no labeling was seen in odontoblasts.

CONCLUSIONSMsx-1, Msx-2 and Dlx-2 are expressed in various patterns during murine mandibular first molar development, suggesting they possibly play a role in the interaction between the epithelium and mesenchyme during the molar development.

Animals ; DNA-Binding Proteins ; genetics ; Female ; Gene Expression Regulation, Developmental ; Genes, Homeobox ; Homeodomain Proteins ; genetics ; MSX1 Transcription Factor ; Male ; Mandible ; embryology ; Mice ; Molar ; embryology ; RNA, Messenger ; analysis ; Transcription Factors ; genetics

Angioplasty, Balloon ; methods ; Budd-Chiari Syndrome ; diagnostic imaging ; etiology ; therapy ; Hepatic Veins ; diagnostic imaging ; pathology ; Humans ; Liver ; diagnostic imaging ; pathology ; Liver Circulation ; Liver Transplantation ; adverse effects ; Living Donors ; Male ; Middle Aged ; Radiography ; Radiology, Interventional ; Retrospective Studies ; Treatment Outcome ; Ultrasonography, Doppler, Color