1.Analysis of influencing factors of multi-site work-related musculoskeletal disorders in surgeons
HUANG Kai sheng HUANG Dang sheng WU Yun PENG Zhi heng SHI Yi ming WANG Zhong xu YANG Min
China Occupational Medicine 2022;49(05):493-
Objective - -
To analyze the prevalence and influencing factors of multi site work related musculoskeletal disorders
( ) Methods
WMSDs in surgeons. A total of 102 surgeons from four hospitals were selected as study subjects by convenient
sampling method. The Chinese version of Musculoskeletal Disorders Questionnaire was used to investigate the prevalence of
, Results
WMSDs in the past one year the related individuals and occupational factors. The total prevalence of WMSDs among
( ), ( ) ( )
surgeons was 54.9%. The top three sites were neck 48.0% lower back 35.3% and shoulder 32.4% . The prevalence of
( vs ,P )
WMSDs in multiple sites was higher than that in a single site 43.1% 11.8% <0.01 . Multivariate logistic regression
, ,
analysis showed that surgeons who smoked were tired at work and had a bent back had a higher risk of developing WMSDs
[ ( - ), ( - ), ( - ), P ]
odds ratios and 95% confidence intervals were 3.66 1.41 9.46 8.33 2.15 32.20 and 18.74 2.14 166.77 all <0.01
Conclusion -
after excluding the influence of confounding factors. The prevalence rate of multi site WMSDs among surgeons is
,
high and the influencing factors include bad living habits and occupational factors such as working load and working posture.
2.Characteristics of infrasound and its influence on workers in working environment of certain thermoelectricity works and department.
Wei-Min DANG ; Sheng WANG ; Shi-Xiu TIAN ; Quan-Hong ZHAO ; Bing CHEN ; Fei SUN ; Li-Hua HE ; Zhi-Fang ZOU ; Zhi-Bin GUO ; Wen-Jun MA
Chinese Journal of Industrial Hygiene and Occupational Diseases 2008;26(12):711-714
OBJECTIVETo study the characteristics of infrasound and its effects on the workers at power plants.
METHODSThe audible noise and infrasound in three thermoelectricity plants were measured and by using Symptom Checklist 90 (SCL-90), the subjective sensation, and the physiological indices were compared between exposed workers and controls.
RESULTSThe infrasound levels were different at different measure sites of the three thermoelectricity works ranging from 40 to 98 dB. There was still 40 approximately 80 dB infrasound even when the soot blower stopped running. Different apparatus produced different infrasound levels with the highest ranging from 62 to 115 dB. A single frequency (16 Hz) infrasound was produced in certain department during working hour with sound pressure levels of 110 to 120 dB, but the audible noise sound pressure level was less than 70 dB. There was no significant difference in the indices representing vision fatigue and neurobehaviour function between exposed workers and controls. Workers at certain department experienced evident subjective sensation of neurobehavioral dysfunction, and the scores of somatization, depression, hostility, phobic anxiety, and psychotism in the SCL-90 were significantly higher in the exposed group than in the control and the norm in China (P < 0.05).
CONCLUSIONInfrasound is ubiquitous in the working environment, but usually, the noise levels are less than 120 dB. In some special production department, there is persistent infrasound above 110 to 120 dB. No obvious health effects are found among those who are exposed to infrasound below 100 dB. However, the workers who are chronically exposed to infrasound above 110 to 120 dB present notable subjective sensation of autonomic neurobehavioral dysfunction, and their psychological health status is not as good as those in the control and those in the domestic normal pattern.
Adult ; China ; Humans ; Occupational Exposure ; adverse effects ; Power Plants ; Sound ; adverse effects ; Workplace
3.Prokaryotic expression, purification and activity assay of recombinant vascular endothelial growth factor.
Pei YANG ; Kun-zheng WANG ; Zhi-bin SHI ; Xiao-qian DANG ; Peng-bo YU ; Chun-sheng WANG ; Fu-liang GONG
Journal of Southern Medical University 2006;26(9):1263-1268
OBJECTIVETo express human vascular endothelial growth factor (hVEGF(165)) in E. coli JM109 in the form of fusion protein by genetic engineering and test the biological activity and immunological competence of the expressed protein.
METHODShVEGF(165) gene was subcloned by PCR and inserted into pQE30 plasmid. hVEGF(165) fusion protein was expressed in E. coli JM109 and purified by Ni(2+)-NTA. The immunological competence of the expressed protein was tested by means of Western blotting and enzyme-linked immunosorbent assay (ELISA), and its biological activity was assayed by chicken chorioallantoic membrane (CAM) and Matrigel angiogenesis assay.
RESULTSThe recombinant hVEGF(165) fusion protein was successfully expressed in E. coli JM109 and its expression accounted for 30% of the total cellular protein. The purified protein presented a single band of 23 kD in SDS-PAGE. Western blotting, ELISA, CAM and matrigel angiogenesis assay showed excellent immunologic competence and biological activity of the recombinant protein.
CONCLUSIONRecombinant hVEGF(165) protein with excellent biological activity has been successfully expressed in E.coli JM109, which may facilitate future study in construction of prefabricated tissue-engineered bone graft.
Blotting, Western ; Cloning, Molecular ; Enzyme-Linked Immunosorbent Assay ; Escherichia coli ; genetics ; metabolism ; Humans ; Plasmids ; genetics ; Prokaryotic Cells ; metabolism ; Recombinant Proteins ; biosynthesis ; genetics ; isolation & purification ; Vascular Endothelial Growth Factor A ; biosynthesis ; genetics ; isolation & purification
4.Ubiquitin ligase Smurf1 promotes poly-ubiquitination of PDK1
Jun WEI ; Hui-Sen SHI ; Zhi-Sheng DANG ; Ling-Qiang ZHANG ; Xue-Li ZHANG
Military Medical Sciences 2017;41(12):947-951
Objective To investigate the mechanism of 3-phosphoinositide-dependent protein kinase 1(PDK1)poly-ubiquitination.Methods Co-immunoprecipitation(Co-IP)and Western blot(WB)were used to analyze poly-ubiquitination of PDK1.It was confirmed that ubiquitin ligase smad ubiquitylation regulatory factor 1(Smurf1)inprove PDK1 poly-ubiquitination within MEF cells,site-directed mutagenesis and WB before PDK1 poly-ubiquitination sites were determined.Results We found that PDK1 could undergoes poly-ubiquitination,ubiquitin ligase Smurf1 was found to be a direct E3 ligase for PDK1 poly-ubiquitination and might rely on the ubiquitin ligase Smurf 1 K699 site activity.K304 was PDK1 poly-ubiquitination modification site point.Conclusion The ubiquitin ligase Smurf1 can promate poly-ubiquitination of PDK1.
5.Study on the ARIMA model application to predict echinococcosis cases in China
En-Li TAN ; Zheng-Feng WANG ; Wen-Ce ZHOU ; Shi-Zhu LI ; Yan LU ; Lin AI ; Yu-Chun CAI ; Xue-Jiao TENG ; Shun-Xian ZHANG ; Zhi-Sheng DANG ; Chun-Li YANG ; Jia-Xu CHEN ; Wei HU ; Xiao-Nong ZHOU ; Li-Guang TIAN
Chinese Journal of Schistosomiasis Control 2018;30(1):47-53
Objective To predict the monthly reported echinococcosis cases in China with the autoregressive integrated mov-ing average(ARIMA)model,so as to provide a reference for prevention and control of echinococcosis. Methods SPSS 24.0 software was used to construct the ARIMA models based on the monthly reported echinococcosis cases of time series from 2007 to 2015 and 2007 to 2014,respectively,and the accuracies of the two ARIMA models were compared. Results The model based on the data of the monthly reported cases of echinococcosis in China from 2007 to 2015 was ARIMA(1,0,0)(1,1, 0)12,the relative error among reported cases and predicted cases was-13.97%,AR(1)=0.367(t=3.816,P<0.001),SAR (1)=-0.328(t=-3.361,P=0.001),and Ljung-Box Q=14.119(df=16,P=0.590).The model based on the data of the monthly reported cases of echinococcosis in China from 2007 to 2014 was ARIMA(1,0,0)(1,0,1)12,the relative error among reported cases and predicted cases was 0.56%,AR(1)=0.413(t=4.244,P<0.001),SAR(1)=0.809(t=9.584, P<0.001),SMA(1)=0.356(t=2.278,P=0.025),and Ljung-Box Q=18.924(df=15,P=0.217).Conclusions The different time series may have different ARIMA models as for the same infectious diseases.It is needed to be further verified that the more data are accumulated,the shorter time of predication is,and the smaller the average of the relative error is.The estab-lishment and prediction of an ARIMA model is a dynamic process that needs to be adjusted and optimized continuously accord-ing to the accumulated data,meantime,we should give full consideration to the intensity of the work related to infectious diseas-es reported(such as disease census and special investigation).
6.Effect of Shaoyaotang on Expressions of CD14, FADD and Caspase-8 in Colonic Tissues of Rats with Large Intestinal Damp-heat Syndrome of Ulcerative Colitis
Si-qi CAO ; Feng-yi WANG ; Sheng-nan TANG ; Dang-sheng ZHAO ; Yang-yang LI ; Zhi-jie LIU ; Rui-ting CHAI
Chinese Journal of Experimental Traditional Medical Formulae 2021;27(5):1-7
Objective:To observe the effect of Shaoyaotang on the contents of cell adhesion molecule-1 (ICAM-1) and transforming growth factor-
7.Current Status of Antithrombotic Strategy for Elderly Patients With Atrial Fibrillation and Acute Coronary Syndrome After Stent Implantation in Beijing Area
Bo-Yang ZHANG ; Peng-Fei LIU ; Nan-Nan WANG ; Yu-Bin WANG ; Ru-Chen LIU ; Ge WANG ; Xiu-Feng XIE ; Hai-Feng YUAN ; Hai-Hong TANG ; Li ZHAO ; Yun-Dai CHEN ; Kang-Xing SONG ; Yi-Da TANG ; Cheng-Jun GUO ; Yun-Tian LI ; Zhi-Min MA ; Xian WANG ; Dang-Sheng HUANG ; Shou-Li WANG ; Tian-Chang LI
Chinese Circulation Journal 2018;33(1):30-35
Objective: To investigate the current status of antithrombotic strategy for elderly patients with atrial fibrillation (AF) and acute coronary syndrome (ACS) after stent implantation in Beijing area and to study the safety and efficacy of different therapeutic strategy. Methods: A total of 467 relevant patients were enrolled by re-travelling electronic medical records from 12 hospitals in Beijing area. The patients' mean age was (78.70±3.32) years and they were divided into 2 groups by antithrombotic therapy condition: Triple therapy group, n=17 (3.64%), Double therapy group, n=450 (96.36%). The incidence of major adverse cardiac and cerebral events (MACCE) including all-caused death, non-fatal myocardial infarction, stent thrombosis, target vessel revascularization (TVR), stoke and bleeding was compared between Triple therapy group and Double therapy group.Results: The medication in Double therapy group included aspirin+ticagrelor, aspirin+clopidogrel, clopidogrel+warfarin and cilostazol+clopidogrel; in Triple therapy group was aspirin+clopidogrel+warfarin. Patient with HAS-BLED score≥3 was defined as high risk of bleeding and they were all treated by double therapy; HAS-BLED<3 was defined as low risk of bleeding, only 5.03% patients were treated by triple therapy. 3 patients in Triple therapy group and 33 in Double therapy group suffered from gastrointestinal bleeding, P=0.338; 6 patients in Triple therapy group and 128 in Double therapy group had MACCE, P=0.589; 3 and 80 patients died in Triple therapy group and Double therapy group, P=0.766. Conclusion: Triple therapy was rarely used in elderly AF and ACS patients after stent implantation, double therapy was the main strategy; the incidence of MACCE and mortality were similar between triple and double therapies; patients with triple therapy had the higher incidence of gastrointestinal bleeding.
8.Prognostic Factors and Predictive Value of MRD before Consolidation Therapy in Middle-risk Newly Diagnosed Young and Middle-aged Patients with AML.
Zhi-Ying NIU ; Hui-Bing DANG ; Lei YUE ; Qiu-Sheng TIAN
Journal of Experimental Hematology 2021;29(2):462-468
OBJECTIVE:
To explore the prognostic factors of young and middle-aged patients with acute myeloid leukemia (AML) and the predictive value of minimal residual disease (MRD) before consolidation therapy.
METHODS:
The clinical data of 262 middle-risk young and middle-aged patients with AML treated in our hospital from January 2010 to December 2018 were selected retrospectively. All the patients were reached morphological leukemia-free state (MLFS) after induction chemotherapy, the overall and subgroup clinical data of the selected patients were analyzed. Cox regression model was used to evaluate the independent prognostic factors of middle-risk newly diagnosed young and middle-aged patients.
RESULTS:
Among the patients less than 40 years old treated by consolidation therapy with PR-CT and allo-HSCT regimens, the 5-year cumulative leukemia-free survival(LFS) rates were 40.92% and 63.51%(P=0.01)respectively, while those over 40 years old were 23.61% and 49.14%(P=0.00), respectively. The 5-year cumulative LFS rates of the patients treated by chemotherapy and achieved early remission and late remission were 63.51% and 41.33% (P=0.01), respectively. The 5-year cumulative overall survival(OS) rates of the patients treated by PR-CT and allo-HSCT regimens were 23.65% and 69.32% (P=0.00), respectively, and the 5-year cumulative LFS rates were 26.44% and 52.30% (P=0.01). Among the patients treated by PR-CT consolidation treatment, the MRD-negative and MRD-positive cases were 74 and 60 cases, respectively. The 5-year cumulative incidence of relapse rate in the MRD-negative subgroup was significantly lower than those in the MRD-positive subgroup (P<0.05), the 5-year LFS rate and OS rate of the patients in MRD-negative subgroup were significantly higher than those in MRD-positive subgroup (P<0.05). For the patients treated by allo-HSCT consolidation treatment, the MRD-negative and MRD-positive cases were 66 and 62 cases, respectively. The 5-year cumulative incidence of relapse rate of the patients in MRD-negative subgroup was significantly lower than those in MRD-positive subgroup(P<0.05), and the 5-year LFS and OS rates of the patients in MRD-negative subgroup were significantly higher than those in MRD-positive subgroup (P<0.05). The univariate analysis results showed that age, chromosome karyotype, MRD status after reaching MLFS, and consolidation treatment regime were all related to the prognosis of patients (P<0.05). The multivariate analysis results showed that age, MRD status after reaching MLFS, and consolidation therapy were the independent factors affecting the cumulative OS rate of the patients (P<0.05). Chromosome karyotype was an independent factor affecting the cumulative LFS rate of the patients (P<0.05). MRD status and consolidation treatment plan after reaching MLFS were the independent factors affecting the cumulative recurrence rate of the patients (P<0.05).
CONCLUSION
The OS rate of middle-risk young and middle-aged patients with newly diagnosed AML is independently related to age, MRD status after MLFS and consolidation therapy, while chromosome karyotype is independently related to cumulative LFS, and allo-HSCT consolidation therapy is recommended for middle-risk young and middle-aged AML patients after induction chemotherapy for MLFS, especially for those less than 40 years old and MRD positive before consolidation therapy.
Adult
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Consolidation Chemotherapy
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Hematopoietic Stem Cell Transplantation
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Humans
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Leukemia, Myeloid, Acute
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Middle Aged
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Neoplasm, Residual
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Prognosis
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Retrospective Studies
9.Effect of Shaoyaotang on Expressions of TLR4, NF-κB p65 and IL-6 in Rats with Damp-heat Ulcerative Colitis
Min XU ; Feng-yi WANG ; Dang-sheng ZHAO ; Xiao-wei PU ; Lei ZHANG ; Xiao-yuan ZHANG ; Lin LI ; Shu-xia LI ; Zhi-ping LI
Chinese Journal of Experimental Traditional Medical Formulae 2020;26(14):53-58
Objective:To explore the mechanism of Shaoyaotang in the treatment of ulcerative colitis (UC) based on toll-like receptor 4 (TLR4)/nuclear factor kappaB (NF-
10.Preparation instructions for Experts consensus statement on Cheezheng Xiaotong Tiegao in clinical practice.
Jian-Min WEN ; Wei CHEN ; Pei-Fu TANG ; Sheng-Hua LI ; Hong-Sheng ZHAN ; Pei-Jian TONG ; Lei DANG ; Gui-Qin MA ; Zhi-Gang ZHUANG ; Jin JIN ; Jian-Min LI
China Journal of Chinese Materia Medica 2019;44(13):2875-2879
As a topical plaster developed by modern pharmaceutical technology based on traditional Tibetan medicine,Cheezheng Xiaotong Tiegao has functions of promoting blood circulation,relieving swelling and relieving pain. Since its introduction in 1993,it has been widely used in the treatment of various types of acute and chronic musculoskeletal pain and various types of spinal,joint and soft tissue diseases. In order to better standardize the clinical application and improve the clinical efficacy of Cheezheng Xiaotong Tiegao,the research and development work of the Experts consensus statement on Cheezheng Xiaotong Tiegao in clinical practice was officially launched on October 19,2017,upon approval from China Association of Chinese Medicine. In this paper,main R&D process and related technical links for the experts consensus on Cheezheng Xiaotong Tiegao would be summarized,which will help the various medical workers understand,master and apply more accurately,and also provide reference for the development of experts consensus on clinical application of other topical Chinese medicines.
Administration, Topical
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China
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Consensus
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Humans
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Medicine, Tibetan Traditional
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Pain
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Pain Management