1. Liver transplantation in China: Focuses in recent years
Academic Journal of Second Military Medical University 2006;27(10):1045-1047
The past few years have witnessed a rapid development in liver transplantation in China and great achievement has been made. This review introduces the recent focuses on liver transplantation in China, including scoring for end-stage hepatic disease and liver transplantation, liver transplantation for hepatocellular carcinomas, liver re-transplantation, expanding of the donor pool, and post-operative long-term follow-ups.
2.Analysis of macrophage apoptosis induced by Brucella melitensis and the effects of caspases 3, 8 and 9
Xiao-li, REN ; Yuan-zhi, WANG ; Chuang-fu, CHEN ; Ya-li, ZHANG ; Hui, WANG ; Lin, ZHANG
Chinese Journal of Endemiology 2013;32(5):482-485
Objective To determine the difference of macrophage RAW264.7 apoptosis induced by Brucella melitensis virulent strain 16M and attenuated strain M5-90 and elucidate the regulatory role of caspases 3,8 and 9.Methods The best multiplicity of infection (MOI) was determined through kinetic analysis of Brucella melitensis strain 16M and M5-90 induced mouse macrophages apoptosis(bacterium ∶ cell =100 ∶ 1,50 ∶ 1,10 ∶1).The infection model was established using the best MOI =50 ∶ 1.The numbers of in vivo bacteria by colony formation units were calculated after macrophages were infected for different times,including 2,4,8,12,24 and 48 h,and the infected cells were collected.The ratios of apoptosis were detected and the regulation of caspases 3,8 and 9 in apoptosis pathway was elucidated by flow cytometry.Results The numbers of 16M in vivo bacteria were 105.4,104.8,105.8,106.5,108.0 and 109.0,respectively and of M5-90 were 106.1,106.2,106.4,106.3,106.1 and 105.0,respectively.The number of in vivo bacteria of 16M was significantly increased than that of M5-90 after infected for 24 h to 48 h.The ratios of apoptosis induced by 16M after infected for 2,4,8,12,24 and 48 h was (2.67 ± 0.09)%,(13.13 ± 0.30)%,(6.56 ± 0.42)%,(6.49 ± 0.28)%,(16.07 ± 0.86)% and (24.23 ± 1.67)%,respectively,and by M5-90 was (3.62 ± 0.02)%,(32.01 ± 2.59)%,(17.58 ± 0.44)%,(16.09 ± 0.10)%,(62.53 ± 2.70)% and (85.53 ± 0.15)%,respectively,and by control group was [(1.90 ± 0.20)%,(1.92 ±0.16)%,(1.99 ± 0.03)%,(2.48 ± 0.11)%,(3.56 ± 0.07)%,(5.26 ± 0.33)%].The differences were statistically between groups in same time.The Brucella melitensis vaccine strain M5-90 was more powerful than virulent strain 16M in respect of inducing macrophage apoptosis after infected for 24 to 48 h.Twenty-four hours after infection,the expression of caspases 3,8 and 9 was (1.47 ± 0.05)%,(1.52 ± 0.02)% and (2.47 ± 0.12)%,respectively,in control group and the expression was (9.70 ± 0.46)%,(6.08 ± 0.56)% and (35.08 ± 1.64)%,respectively,after infected for 24 h induced by M5-90.The expression of caspases 3,8 and 9 was significantly higher than that control group (P < 0.01).Twenty-four hours after given caspases 3,8 and 9 inhibitor,apoptosis rate in control group was (66.72 ± 1.28)%,in M5-90 group was (22.58 ± 0.55)%,(53.15 ± 1.85)% and (29.18 ± 0.23)%,respectively,and compared with control group,apoptosis rate of caspases 3,8 and 9 was significantly lower(P < 0.01).Conclusions Apoptosis of macrophage can be induced by Brucella melitensis virulent vaccine strain 16M and attenuated strain M5-90.M5-90 is stronger than that of strain 16M.Caspases 3,8 and 9 can regulate macrophage apoptosis after M5-90 infection.
3.Effect of serum hyaluronic acid on the liver cryopreservation-reperfusion injury in rats
Sui-Feng LÜ ; Zhi-Ren FU ; Guan-Nan XU ; Meng-Long WANG
Academic Journal of Second Military Medical University 2001;22(1):77-79
Objective: To investigate the relation between ser um hyaluronic acid (HA) concentration and cryopreservation-reperfusion injury. Methods: The animals were randomly assigned to 3 groups: (1 ) group A: the control; (2) group B: liver allografts were stored in lactated R inger's solution (0℃) for 2 h before implantation; (3) group C:liver allografts were stored in lactated Ringer's solution (0℃) for 4 h before implantation. Th e serum sample and liver specimen were taken up at 2 h and 4 h after transplanta tion to detect the concentration of HA, AST and LDH, and to get pathologic obser vation. Results: Serum HA increased earlier and decreased more s hortly than AST and LDH after transplantation in group A. Serum HA increased sig nificantly in group B and C, much higher than that in group A(P<0.01). The i njury of vascular endothelium and the disorder of hepatic sinuses and hepatic lo b ules were observed in group B and C. In the specimen of 4 h in group C, evident infiltration of inflammatory cell was present. Conclusion: Cryopreservation leads to injury of endothelial cell and reperfusion aggravat es this injury. The serum HA concentration indicates the degree of cold ischemia -reperfusion injury.
4.Study on the mutations of ATP2C1 gene in patients with Harley-Harley disease
Xiao-Xiao YAN ; De-Ke JIANG ; Fu-Ren ZHANG ; Hong-Qing TIAN ; Gui-Zhi ZHOU ; Xian-Mei LU ; Long YU
Chinese Journal of Dermatology 1994;0(06):-
Objective To detect the mutations of ATP2C1 gene in patients with Hailey-Hailey dis- ease (HHD).Methods PCR and direct sequencing were performed in 17 patients and 120 healthy controls to screen the mutations in the exons of ATP2C1 gene.Results Eight mutations were identified in nine probands, including three deletion mutations (nt1464-1487 del/nt1462-1485del,1523delAT,2375delTTGT),three splice site mutations (360—2A→G,1415—2A→T,2243+2T→C) and two missence mutations (C920T and G1942T).None of the above mutations was found in the controls.Conclusion Eight specific novel mutations were identified in nine probands of HHD,which could be causative factors of the disease.
5.Researches on Morphology and Anatomy of Root System of Dendrobium nobile Lindl.
Ming ZHANG ; Fu-Jun YIN ; Shi-Jiang CHEN ; Zhi-Long BIE ; Ling-Yan REN
China Journal of Chinese Materia Medica 2001;26(6):284-285
Objective: To understand the morphological and anatomical characteriatics of Dendrobium nobile so as to provide scientific basis for its domestication and culivation. Method: The root's morphological development and interior structure were investigated. Result and conclusion: Dendrobium has peculiar structure which determines that this plant requires a growing environment with high water and air ventilation properties.
6.Expression of heparanase mRNA and its clinical significance in primary hepatocellular carcinoma.
You-lei ZHANG ; Zhi-ren FU ; Guo-shan DING ; Hong FU ; Yuan-he WANG ; Qiang WANG
Chinese Journal of Oncology 2003;25(4):359-361
OBJECTIVETo investigate the expression of heparanase mRNA and its relation with the clinicopathological features and angiogenesis in primary hepatocellular carcinoma (HCC).
METHODSExpression of heparanase mRNA was detected by RT-PCR in 51 HCC lesions, and microvessel density (MVD) was detected by immunohistochemical stain with a factor VIII-related monoclonal antibody.
RESULTSExpression of heparanase mRNA was shown in 49.0% (25/51) HCC lesions. The positive rate of heparanase expression in tumors larger than 3 cm (63.6%, 21/33) was significantly higher than those in smaller tumors (22.2%, 4/18; P < 0.01). Heparanase expression was more frequent in highly invasive tumors (70.0%, 14/20) compared with moderately invasive tumors (46.7%, 7/15) and low invasive ones (25.0%, 4/16; P < 0.05). Moreover, heparanase expression in tumors with high MVD (62.5%, 20/32) was significantly higher than those in tumors with low MVD (26.3%, 5/19; P < 0.05).
CONCLUSIONHeparanase mRNA expression may be important for the growth, invasion and angiogenesis of hepatocellular carcinoma.
Adult ; Carcinoma, Hepatocellular ; blood supply ; enzymology ; pathology ; Female ; Glucuronidase ; biosynthesis ; genetics ; Humans ; Liver ; enzymology ; Liver Neoplasms ; blood supply ; enzymology ; pathology ; Male ; Microcirculation ; pathology ; Middle Aged ; Neoplasm Invasiveness ; Neovascularization, Pathologic ; enzymology ; RNA, Messenger ; biosynthesis ; genetics ; Tumor Burden
7.Clinical analysis of liver transplantation in elderly patients.
Wei LIU ; Hong FU ; Wei-yuan GUO ; Jun MA ; Zhi-ren FU ; Guo-shan DING
Chinese Journal of Hepatology 2008;16(5):389-390
Adult
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Aged
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Humans
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Liver Transplantation
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Male
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Middle Aged
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Prognosis
8.The effect of combination of glycine and methylprednisolone on Kupffer cells of liver after hemorrhagic shock in rats.
Gang WANG ; Yang WANG ; Fu-lan GUAN ; Guang-cheng REN ; Yu-zhi WANG
Chinese Journal of Surgery 2006;44(5):349-352
OBJECTIVETo investigate the effect of combination of glycine and methylprednisolone (MP) on Kupffer cells in liver of rats suffered from hemorrhagic shock.
METHODSFifty Wistar rats were bled to establish the shock model and subsequently resuscitated with shed blood and normal saline. Just prior to resuscitation, the rats were randomly assigned to 5 groups: sham group, shock group, shock + glycine group, shock + MP group and shock + glycine + MP group. The intracellular calcium concentration and the level of tumor necrosis factor alpha (TNF alpha) in the culture medium of Kupffer cells were determined after stimulation with different concentrations (1, 10, 100 and 1000 ng/ml) of lipopolysaccharide (LPS).
RESULTSConcentration of intracellular calcium and production of TNF-alpha by isolated Kupffer cells stimulated by LPS were elevated significantly in the rats with hemorrhagic shock, which were totally prevented by glycine + MP compared with other groups (P < 0.005).
CONCLUSIONSThe combination of glycine and MP prevents the increase of intracellular calcium of Kupffer cell, suppress Kupffer cell activation, decrease the production of TNF-alpha of Kupffer cell and block systemic inflammatory responses more effectively than single administer of glycine or MP.
Animals ; Calcium ; metabolism ; Cells, Cultured ; Disease Models, Animal ; Dose-Response Relationship, Drug ; Female ; Glycine ; pharmacology ; therapeutic use ; Kupffer Cells ; drug effects ; pathology ; physiology ; Liver ; drug effects ; metabolism ; pathology ; Male ; Methylprednisolone ; pharmacology ; therapeutic use ; Random Allocation ; Rats ; Rats, Wistar ; Shock, Hemorrhagic ; drug therapy ; pathology ; physiopathology ; Tumor Necrosis Factor-alpha ; metabolism
9.G-protein coupled receptor 34 knockdown impairs the proliferation and migration of HGC-27 gastric cancer cells in vitro.
Zhong-Tian JIN ; Kun LI ; Mei LI ; Zhi-Gang REN ; Fu-Shun WANG ; Ji-Ye ZHU ; Xi-Sheng LENG ; Wei-Dong YU ;
Chinese Medical Journal 2015;128(4):545-549
BACKGROUNDOverexpression of G-protein coupled receptor 34 (GPR34) affects the progression and prognosis of human gastric adenocarcinoma, however, the role of GPR34 in gastric cancer development and progression has not been well-determined. The current study aimed to investigate the effect of GPR34 knockdown on the proliferation, migration, and apoptosis of HGC-27 gastric cancer cells and the underlying mechanisms.
METHODSThe expression of GPR34 in gastric cancer cell line HGC-27 was detected by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. HGC-27 cells were employed to construct the stable GPR34 knockdown cell model in this study. Real-time RT-PCR and Western blotting were applied to validate the effect of short hairpin RNA (ShRNA) on the expression of GPR34 in HGC-27 gastric cells. The proliferation, migration of these cells were examined by Cell Counting Kit-8 and transwell. We also measured expression profile of PI3K/PDK1/AKT and ERK using Western blotting.
RESULTSThe ShRNA directed against GPR34 effectively inhibited both endogenous mRNA and protein expression levels of GPR34, and significantly down-regulated the expression of PIK3CB (P < 0.01), PIK3CD (P < 0.01), PDK1 (P < 0.01), phosphorylation of PDK1 (P < 0.01), Akt (P < 0.01), and ERK (P < 0.01). Furthermore, GPR34 knockdown resulted in an obvious reduction in HGC-27 cancer cell proliferation and migration activity (P < 0.01).
CONCLUSIONSGPR34 knockdown impairs the proliferation and migration of HGC-27 gastric cancer cells in vitro and provides a potential implication for therapy of gastric cancer.
Apoptosis ; genetics ; physiology ; Blotting, Western ; Cell Line, Tumor ; Cell Proliferation ; genetics ; physiology ; Humans ; RNA, Small Interfering ; genetics ; Real-Time Polymerase Chain Reaction ; Receptors, Lysophospholipid ; genetics ; metabolism ; Stomach Neoplasms ; genetics ; metabolism
10.The role of glycogen synthase kinase-3 beta in the pathogenesis of liver ischemia reperfusion injury.
Feng REN ; Hai-yan ZHANG ; Zheng-fu PIAO ; Su-jun ZHENG ; Yu CHEN ; Zhi-ming WU ; Zhong-ping DUAN
Chinese Journal of Hepatology 2011;19(7):547-551
OBJECTIVETo investigate the role of the key intracellular signaling molecule glycogen synthase kinase-3 beta in the mechanism of liver ischemia reperfusion (IR).
METHODSC57BL/6 mice were subjected to 90 min warm liver cephalad lobe ischemia, followed by various length of reperfusion. Experiment groups included sham control group, liver IRI model group and glycogen synthase kinase-3 beta inhibitor-treated group (SB216763 in DMSO, 25 g/kg, i.p, 2 hour prior to the onset of liver ischemia). The expression of glycogen synthase kinase-3 beta protein was analysed by Western blotting. The serum ALT levels were determined to reflect the function of liver. The affected liver lobes were harvested for histology analysis. The inflammatory gene expression was detected by Quantitative PCR.
RESULTSBy western blot analysis, we found that ischemia itself activated glycogen synthase kinase-3 beta by a significant decrease of its phosphorylation. Glycogen synthase kinase-3 beta inhibitor SB216763-pretreatment ameliorated the liver damages significantly as compared to the controls (sALT: 2046+/-513 U/L vs 5809+/-1689 U/L, P = 0.0153), and suppressed the gene expressions of IL-12, TNFa, IL-1b and IL-6.
CONCLUSIONSThis study demonstrated that the ischemia process modulated liver innate immune activation via a GSK-3-dependent mechanism which favored the development of a pro-inflammation response and lead to liver tissue damages. GSK-3b may be a new therapeutic target to ameliorate liver IRI in transplant patients.
Animals ; Glycogen Synthase Kinase 3 ; metabolism ; Glycogen Synthase Kinase 3 beta ; Inflammation ; metabolism ; Liver ; metabolism ; pathology ; Male ; Mice ; Mice, Inbred C57BL ; Reperfusion Injury ; metabolism ; pathology