Objective This study aims to observe and analyze the result of implementing health management and intervention for chronic Keshan disease treatment. Methods Certain amounts of chronic Keshan disease patients in Keshan disease area were chosen and given health management and treatment intervention including primarily pharmacological treatment for the chronic congestive heart failure together with health education and life style instruction. From 2006 to 2008, three interventions were conducted every six month. Electrocardiogram description, proportion of heart to chest, classification of heart function, types of Keshan disease and labour capacity at the initial and the last stage of the intervention were taken as judging indexes. If one of the indexes was improved then the intervention will be regarded as effective. The number of effective cases and effective rate were counted in order to observe the impact of intervention times and different medicine on intervention results. Results Altogether 345 Keshan disease patients were involved in the study, among them 207 were effective, the effective rate was 60%(207/345). One hundred and eighty were involved in the intervention for once, and the effective rate was 44%(79/180). Ninty-one took part in the intervention twice, and the effective rate was 66%(60/91). Seventyfour attended the intervention for three times, and the effective rate was 92% (68/74). There were significant difference among groups of different intervention times (χ2 = 52.16, P < 0.01), and the effective rate increased with increased intervention times. Three times were higher than that of one or two times (χ2= 15.81, 49.56, all P< 0.0125). Twice were better than once(χ2 = 11.76, P < 0.0125). There were significant difference among groups of different medicine groups(χ2 = 19.56, P< 0.01). Among different medicine groups, the efficacy of angiotensin converting enzyme inhibitor(ACEI) group was the highest 77%(82/106) while the cardiotonic medicine group was 50%(47/94) and cardiac muscle nutrition poisons was 54% (78/145). The efficacy rate of ACEI group was higher than that of cardiotonic medicine group and cardiac muscle nutrition poisons(χ2 = 16.28, 14.71, P< 0.0125). Conclusions The implement of health management and treatment intervention to Keshan disease patients is of great significant in improving the healthy condition of Keshan disease patients. Health education and life-style instruction as well as the use of ACEI, β-blockers and diuretic have comparatively obvious effect on treatment of chronic Keshan disease congestive cardiac failure.

Objective: To establish a mouse hepatic cancer cell line Hca-F transfected with shRNA (small hairpin RNA) targeting annexin A7, so as to provide a basis for future study. Methods: Three shRNAs(shRNA1, 2 and 3) were designed and inserted into the pSilencer vector to silence annexin A7 gene. The three pSilencer-shRNA vectors were transfected into Hca-F cells separately, and the most effective pSilencer-shRNA vector was selected based on the results of RT-PCR and Western blotting. The Hca-F cells were transfected with the most effective pSilencer-shRNA vector and the transfectants were selected by 400 μg/ml G418. The cells with annexin A7 stably knockdown were passaged and the expression of annexin A7 was confirmed by Western blotting, and the result was compared with those transfected with empty vector and normal controls. Results: The sequencing results confirmed that the sequences of the 3 shRNAs were correct,and shRNA1 was found to have the best inhibitory effect against annexin A7. Compared with normal Hca-F cells and those transfected with empty vectors, the annexin A7 protein expression was significantly down-regulated in cells transfected with pSilencer-shRNA(0.318 6 vs 0.824 3, 0.798 7,P <0.05), with no significant difference found between the former 2 groups. Conclusion: We have successfully established a Hca-F cell line with annexin A7 stably down-regulated using shRNA technique, paving a way for future study.

Mesenchymal Stem Cell Transplantation ; Mesenchymal Stromal Cells ; cytology ; Nervous System Diseases ; surgery

Objective To comprehend the action mechanism of simvastatin in pulmonary hypertension(PH)when it induced by high pulmonary blood flow.Methods Abdominal aorta-inferior vena cava shunting was made in rats to establish animal model of PH induced by high pulmonary blood flow,simvastatin with dose of 2 mg/(kg?d)was used to interfere for 11 weeks.And then,pulmonary arterial pressure,apoptosis rate and proliferation rate of pulmonary vascular smooth muscle cell were determined.Results were compared with other groups.Results Simvastatin could cut down the pulmonary arterial pressure well,pulmonary arterial pressures of simvastatin group rats were lower than those of spliting groups obviously(Pa

Objective To study TIMI flow influence of intracoronary injection of GPⅡb/Ⅲa receptor antagonists (tirofiban) in patients with STEMI before primary PCI. Methods Sixty-seven STEMI patients (48 male, mean age 57.4?8.1 years old) admitted during April, 2005 to August, 2007 were included in the study and in all of them, IRA thrombus was found during CAG. The patients were divided into the primary PCI group (n=32) and primary PCI plus introcoronary tirofiban injection group (n=35). Changes in TIMI flow were were obsurved diving the operation. Results The incidence of slow-flow and no-reflow of the intracoronary tirofiban injection+PCI group was lower than that of the primary PCI group (2.86% vs 18.75%,P

Adenocarcinoma ; metabolism ; pathology ; Adenocarcinoma, Clear Cell ; metabolism ; pathology ; Biomarkers ; metabolism ; Carcinoma ; metabolism ; pathology ; surgery ; Diagnosis, Differential ; Female ; Humans ; Keratins ; metabolism ; Membrane Proteins ; metabolism ; Middle Aged ; Mucin-1 ; metabolism ; Myoepithelioma ; metabolism ; pathology ; surgery ; Neoplasm Recurrence, Local ; Parotid Gland ; surgery ; Parotid Neoplasms ; metabolism ; pathology ; surgery ; S100 Proteins ; metabolism

In order to cultivate advanced person with ability,we should teach students some theoretics and more practical skill during microbiology teaching. after we using the measures “examining on theoretics and practical skill that must be mastering in the lesson”. Students had been more interested in microbiology,and advanced of knowledge. So “examining on theoretics and practical skill that must be mastering in the lesson” is better method in teaching of Microbiology in Occupation technique college.

Humans ; Infant ; Male ; Mycobacterium tuberculosis ; Otitis Media ; microbiology ; Tuberculosis

Objective To identify the arteriosclerosis (AS)changes in peripheral artery and abdominal aorta of patients with familial hypercholesterolemia(FH) during follow-up.Methods Seventeen patients of 6 FH [5 male and 12 female with average age of (16.12?6.65) years old],along with 17 subjects of matching sexes and ages with normal blood cholesterol as healthy control group,underwent examination by color doppler ultrasound,and changes of intima-media thickness (IMT) in peripheral artery and abdominal aorta,severity of stenosis,morphology,and function were observed.Results For 17 patients of FH,the total cholesterol(TC),low density lipoprotein cholesterol(LDL-C) in serum were higher significantly and high density lipoprotein cholesterol (HDL-C) was lower significantly than those in healthy control group(Pa0.05).The IMT of carotid aorta,subclavicular aorta,common abdominal artery,and common femoral artery in 13 patients were showing various degrees of increase,yielding an average of 2.9 mm.As patients aged,this phenomenon was reported to be more profound in common abdominal aorta and femoral artery.Conclusions Patients of FH show AS lesion in early stage and it worsened as they aged,from carotid arteries to common abdominal aorta and femoral artery.Color doppler ultrasound can be a non-invasive examination for monitoring the progress of AS in blood vessels in patients of diagnosed FH.