Objective: To assess the clinical effect of modified Mapleson D breathing system in intravenous anesthesia for minor surgical procedures. Methods: A total of 112 patients (ASA I-II) scheduled for minor outpatient surgery were randomly divided into two groups, with 56 in each group. Patients in Group A inhaled oxygen using common face mask, and were transferred to artificial ventilation by emergency ventilator when respiratory depression occurred; those in Group B were subjected to modified Mapleson D breathing system, and were transferred to artificial ventilation using the present breathing system when respiratory depression occurred. The respiratory rate (RR), oxygen saturation (SpO 2), Partial end-tidal CO2 pressure (PETCO 2), occurrence (T1) and rectification time (T2) of respiratory depression were all recorded in the two groups. Results: The incidence of respiratory depression in Group B was lower than that in Group A; moreover, Group B also had a higher incidence of hyoxemia and shorter rectification time compared with Group A. Conclusion: Compared with conventional face mask, our modified Mapleson D breathing system is easy and convenient to use, and it leads to lower incidence and quick rectification of respiratory depression, showing a potential in the airway management of patients undergoing minor intravenous anesthesia.

Objective: To study the effect of obstructive jaundice on liver function and pathomorphology in rats. Methods: Sixty healthy male Sprague-Dawley rats were randomly divided into bile duct ligation (BDL) group (the common bile ducts were ligated, n = 50) and sham group(the common bile ducts were isolated but not ligated, n=10). According to the ligation periods, BDL group was further divided into BDL3, BDL7, BDL14, BDL42 and BDL42 groups. Liver function indices, including the serum total bilirubin(TBIL), direct bilirubin(DBIL), total bile acid(TBA), and alanine aminotransferase(ALT), were measured; and the liver pathomorphology was observed 3, 7, 14, 28, and 42 days after BDL. The above examinations were performed in the sham rats 7 days after operation. Results: The liver function of rats had obvious changes compared with that of sham group. The serum levels of TBIL, DBIL, and TBA reached the peak in the early phases (BDL4 or BDL7) and decreased gradually thereafter, but were still higher than that of the sham control in the end phase(BDL42). There was a similar change in the biochemical indices such as ALT. The main pathomorphology changes in BDL group were intrahepatic bile duct proliferation and hepatic fibrosis. Conclusion: There is obvious impairment in the early phase of obstructive jaundice: the liver function may be improved to a certain degree by a compensation mechanism: however 9 the persistence of obstructive jaundice finally leads to hepatic fibrosis. The liver function indicated by the pathomorphology changes can not reflect the actual impairment of liver tissue in obstructive jaundice rats.

Animals ; Cholesterol ; blood ; Hyperlipidemias ; blood ; drug therapy ; Leptin ; pharmacology ; therapeutic use ; Male ; Mice ; Mice, Inbred Strains

Aged ; Aged, 80 and over ; Coronary Artery Disease ; complications ; therapy ; Coronary Stenosis ; complications ; therapy ; Female ; Humans ; Male ; Middle Aged ; Percutaneous Coronary Intervention

ObjectiveTo seek a convenient method for improving the clinical therapeutic effect on knee osteoarthritis.Method One hundred and eighty-six patients were randomly allocated to three groups, 62 cases each. The observation group received needle-sticking warm needling moxibustion plus articular cavity injection of sodium hyaluronate; control group 1, needle-sticking warm needling moxibustion; control group 2, articular cavity injection of sodium hyaluronate. Needle-sticking warm needling moxibustion was given once daily, five treatments followed by two days of rest, seven days as a course, for a total of five courses. Articular cavity injection of sodium hyaluronate was administered once a week, for a total of five injections. The therapeutic effects were compared after treatment.ResultA large part of the symptoms and signs resolved, and the cure rate, the cure and marked efficacy rate and the efficacy rate were 40.3%, 87.1% and 95.2%, respectively, in the observation group. Part of thesymptoms and signs resolved in control groups 1 and 2. The cure rate, the cure and marked efficacy rate and the efficacy rate were 24.2%,51.6%and 80.6%, respectively, in control groups 1 and 21.0%, 54.8% and 82.3%, respectively, in control groups 2. There were statistically significant differences in the cure rate (P<0.05), the cure and marked efficacy rate (P<0.01) and the efficacy rate (P<0.05) among the three groups. The therapeutic effect was significantly better in the observation group than in control groups 1 and 2.Conclusion Needle-sticking warm needling moxibustion plus articular cavity injection of sodium hyaluronate is clinically a better way to treat knee osteoarthritis.

Objective:To assess the clinical effect of Multimodal Antiemetic Therapy on Postoperative Nausea and Vomiting in Patients Undergone Gynecological Laparoscopic Surgery. Methods:Ninety gynecologic patients scheduled for laparoscopic Surgery were randomly divided into three groups with 30 cases each. Group A (Multimodal Group) received multimodal antiemetic therapy including the management of using TIVA with propofol for induction and maintenance of anesthesia, avoidance of volatile anesthetics, minimization of opioids, combination of using antiemetics ondansetron 4mg + dexamethasone 2.5mg + droperidol 1.25mg for preventing PONV.Group B (Combination Group)received combination antiemetics therapy. Group C(Control Group)didn't receive special interventions for PONV. Patient demographics,risk profiling,incidence of PONV and patient total satisfaction were observed and compared.Result: The preoperative expected risks of PONV in three groups were high. Group A and B were more effective than Group C in preventing PONV.Compared with Group B, Group A has a higher complete response rate and greater patient satisfaction. Conclusion:In Patients Undergone Gynecological Laparoscopic Surgery,evaluated to have a high risks of PONV preoperatively, multimodal antiemetic therapy has an effective prevention, also associated with a better clinical effect and greater patient satisfaction when compared with combination antiemetics therapy.

The total cDNA obtained through reverse transcription of F. oxysporum CGMCC 0536 mRNA used as template, a fragment about 1.5kb was amplied with oligo(dT)15 primer and a gene specific primer designed on the base of the sequence of both NH2-terminus and the cDNA sequence encoding D-lactonohydrolase of Fusarium oxysporum reported on the NCBI, then the fragment was cloned to the pMD18-T vector and sequenced. The sequence encoding D-lactonohydrolase of F. moniliforme CGMCC 0536 shows a high homology of 90.06% with that of F. oxysporum indicating that the gene encoding D-lactonohydrolase is highly conservative. Two specific primers were designed according to the sequence result, and a fragment, 1146bp, was amplied using hot start PCR with these two specific primers. Subsequently, the resulting products were digested with EcoR I and Sal I and ligated to the pTrc99a vector digested with the same enzymes using T4 DNA ligase. the recombinant plasmid, pTrc99a-LAC, was transformed into Escherichia coli JM109. The two positive clones were induced with IPTG, and enzymes expressed in Escherichia coli JM109, the enzyme activity was about 37U and 41U respectively. The expression products were analyzed by SDS-polyacrylamide gel electrophoresis indicating that about 40kD protein was obtained.
Base Sequence ; Carboxylic Ester Hydrolases ; biosynthesis ; genetics ; Cloning, Molecular ; Electrophoresis, Polyacrylamide Gel ; Escherichia coli ; genetics ; metabolism ; Fungal Proteins ; biosynthesis ; genetics ; Fusarium ; enzymology ; genetics ; Molecular Sequence Data

To study the therapeutic effect of Astragalus injection (AI) in treating early stage diabetic nephropathy (DN) patients. Methods: The total of 136 early diabetic nephropathy patients were randomly divided into two groups, 50 cases in the conventional treated group and 86 in the AI treated group, the therapeutic course being 3 weeks. Levels of plasma endothelin-1 (ET-1), 24 hrs urinary albumin excretion rate (uAER), and platelet granule membrane protein (GMP-140), 6-keto-prostaglandin F1α (6-keto-PGF1α), and thromboxane B2 (TXB2) before and after treatment were determined by radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) respectively. Moreover, the above-mentioned criteria in 26 healthy subjects were also measured for control. Results: The plasma ET-1, GMP-140, TXB2 and uAER levels in DN patients were higher, but 6-keto-PGF1α level was lower than those in healthy subjects. The above elevated criteria in DN patients could be lowered by AI treatment. Conclusion: The pathogenesis and development of DN might be closely associated with the changes of plasma ET-1 level and platelet function. Astragalus could improve the above-mentioned changes in patients of early stage DN.

Human herpes simplex virus 1 (HSV-1) causes facial,ocular,and encephalitic disease and is associated with latent infection and cancer.Here,we developed a means of studying the pathogenesis of HSV-1 infection at the protein level by using the SELDI Protein Chip to detect changes of protein expression in Vero cells cultured in vitro.After infection with HSV-1 and culture for 12,24 or 48 h,cells were harvested and lysed.IMAC3 arrays were applied to SELDI-TOF-MS to detect proteomic differences before and after infection.The chip detected a series of differentially expressed protein peaks.Interestingly,both peaks at 16 912 Da and 17 581 Da corresponded precisely with the molecular mass of ISG 15,which may participate in antiviral activity during the process of infection.Thus,the results we obtained can serve as a basis to study the pathogenesis of HSV-1 and the interaction between the virus and its host.In addition,they can help in the discovery of new therapeutic targets for treatment of HSV-1 infection.