1.The development of biliary tract surgery.
Chinese Journal of Surgery 2006;44(23):1585-1586
2.In Vitro Generation of Hematopoietic Stem Cells from Embryonic Stem Cell to Reconstruct Hematopoiesis
Zhi-xu, HE ; Shao-liang, HUANG ; Qiang, MI ; Jing, HUANG
Journal of Applied Clinical Pediatrics 2008;23(15):1213-1218
Objective To search for a good method for generation of hematopoietic stem cells (HSC) by embryonic stem cells (ESC),and to investigate the potential of HSC derived from ESC to reconstruct hematopoiesis in vivo.Methods Using a three-step method to induce a mice ESC line, E14.1-into HSC.And identifying HSC by flow cytometry analysis cell markers with CD34 +/Sca-1+, then HSC (1×109L-1)from ESC differentiation were injected into severe combined immunodefieency (SCID) mice for observing teratoma formation.To validate function of HSC by colonngenic cells assay and to reconstitute the hematopoiesis in lethally irradiated mice.Results Combining to use more hematopoietic stimulating factor to availably promote the El4.1 cell into embryoid body (EBs) with abundant hematopoietic progenitors.EBs were induced after 14 day to fast differentiate for HSC with peak percentages of CD34+/Sca-1+ cells reached to (13.72±2.07)%.To harvested ceils from EBs by day 14 for second -step HSC differentiation, percent of CD34+/Sca-1+ cells rise to(24.62±2.50) % after day 16 induction.Cloning forming units (CFU) analysis showed that more Erythro -myeloid cloning generation was observed at this period.Cells obtained in the second step are subsequently plated on monolayer of mice bone marrow stromal cells, in the presence of TPO, FLt3 ligand and superoatant of mice fetal liver stromal cells, cultured for additional 15 days, followed fast expansion of CD34+/Sca-1+ to maximally (58.64±4.20 ) % with more CFU-E, CFU-GM and CFU-GEMM population.Wright-Giemsa stain showed that its had the character of hematopoietic progenitors.Cells from the third-step were injected into SCID mice, but no teratomas were recovered in 2 mices after 6 weeks.Positive selection of CD34+/Sca-1+ cells by magnetic sorting from the third - step differentiation were transplanted into 7 lethally irradiated female mice while predominant hematopoietic reconstitution were observed in 10 days after transplantation, with 71.4% successful engraftment rate.And 3 recipients showed that the cell population of the peripheral blood leukocytes,red cells, hemoglobin approached normal index at 40 days after transplant, hut followed relative'slow renew in platelet count.Survival rate of transplant group is 43.0%, compared to 100% mortality in control mice.Karyotyping assays confirmed female mice with XY.Conclusions The results showed that the three-step differentiation and the culture conditions described here good support differentiation of ESC into HSC.HSC derived from ESC were safe without teratomas formation in body, and its can reconstruct hematopoiesis.
4.Fourier Transform Infrared Microspectroscopy of Rat Kidney with Regard to Fa- tal Hyperthermia.
Zhi-jun WANG ; Shan-shan SHEN ; Kai-fei DENG ; Zhi-qiang QIN ; Ping HUANG ; Zhen-yuan WANG
Journal of Forensic Medicine 2015;31(4):257-261
OBJECTIVE:
To observe the chemical groups changing in rat kidney with regard to fatal hyperthermia by Fourier transform infrared microspectroscopy (FTIR-MSP) and to provide a new method to diagnose fatal hyperthermia.
METHODS:
Rats were sacrificed by hyperthermia, brainstem injury, massive hemorrhage and asphyxiation and divided into groups. The renal samples were dissected immediately after death. The data of infrared spectroscopy in glomerulus were measured by FTIR-MSP.
RESULTS:
The absorbances of 3290, 3070, 2850, 1540 and 1396 cm(-1) significantly increased (P < 0.05), and the ratios of Al650/A3290 and A1650/A1540 significantly decreased (P < 0.05) in group of hyperthermia.
CONCLUSION
FTIR-MSP can analyze the changes of chemical groups of kidney as an auxiliary diagnosis for discriminating hyperthermia with other causes of death.
Animals
;
Fever/mortality*
;
Fourier Analysis
;
Kidney/metabolism*
;
Microspectrophotometry
;
Rats
;
Spectroscopy, Fourier Transform Infrared/methods*
5.Colon metastasis of chromophobe renal cell carcinoma with sarcomatoid change
Wei-Ping ZHAO ; Yan-Lan YU ; Zhi-Qiang CHEN ; Xue-Feng HUANG ; Zhi-Gen ZHANG
Chinese Medical Journal 2012;125(18):3352-3354
We present a rare case of colonic metastasis of renal cell carcinoma (RCC) and review the literature.A 54-year-old male was referred to our hospital with a history of bloody stools and fever.A dght kidney tumor measuring about 10 cm in diameter was found by abdominal computed tomography.Right radical nephrectomy and a right hemicolectomy with ileotransversostomy were performed.Pathological diagnosis was chromophobe RCC with sarcomotoid change involving the colon.Chromophobe RCC with sarcomotoid change is very rare.
6.Applied research of intermaxillary fixation screw in the jaw fracture.
Wei HUANG ; Zhi-qiang CAO ; Dong FANG ; Zhen-yu HU
Chinese Journal of Plastic Surgery 2004;20(5):364-365
OBJECTIVETo evaluate and generalize the application of intermaxillary fixation screw in the jaw fracture.
METHODS41 cases of jaw fracture have been treated with intermaxillary fixation screw.
RESULTSBoth function and appearance have recuperated in 40 cases except 1 case has light malocclusion.
CONCLUSIONSApplication of intermaxillary fixation screw advanced the traditional therapy.
Adolescent ; Adult ; Bone Screws ; standards ; Female ; Humans ; Jaw Fractures ; surgery ; Male ; Maxillary Fractures ; surgery ; Middle Aged ; Orthopedic Fixation Devices ; standards ; Reconstructive Surgical Procedures ; instrumentation ; methods ; Treatment Outcome
7.Contrast-enhanced ultrasonography in evaluation of splenic trauma and injury grading and its clinical apllication
Qiao-rong, LIANG ; Chun-yan, HUANG ; Tong, LIANG ; Shi-ming, TAO ; Zhi-qiang, ZENG
Chinese Journal of Medical Ultrasound (Electronic Edition) 2008;5(2):288-294
Objective To evaluate the conventional ultrasound (US) and contrast-enhanced ultrasound (CEUS) in diagnosis of splenic trauma including its grading diagnosis. Methods US and CEUS in 42 patients with splenic trauma confirmed by CT and/or operation were performed during 9.2004-10.2007. All the data were compared and analyzed retrospectively. Results Of 42 patients with splenic trauma, 28 cases were detected and 14 cases were missed on US examination, whereas, 40 cases were detected and only 2 mild cases were missed on CEUS examination. The detection rate of lesions with CEUS was significantly higher than that of US (P<0.001) . Ten cases in grading the injury were underestimated by US, however, none of them were underestimated by CEUS. CEUS had good concordance with CT and/or operation in grading diagnosis of 42 cases except two mild cases. Conclusions CEUS has very good concordance with CT and/or operation in detecting injury and grading the splenic trauma compared with conventional US. CEUS as a new imaging technology has made great advance of ultrasoongraphy in evaluation of splenic trauma including injury grading,and it is very useful in clinical application.
8.Analysis of TGFBI gene mutation in a Chinese family with Avellino corneal dystrophies
Zhi-qiang, HOU ; Wei, WANG ; Jing, ZHANG ; Yong-gen, XU ; Zhen, ZHOU ; Jing, HAN ; Chen, HUANG
Chinese Journal of Experimental Ophthalmology 2011;29(3):254-257
Background Researches demonstrated that corneal dystrophy is associated with the mutation of transforming growth factor beta induced gene(TGFBI)located at chromosome 5q31 domine.Recent study showed that the gene mutation location is in R124H of TGFBI gene. Objective This study was to identify the mutation characteristics of TGFBI gene in a Chinese family with Avellino corneal dystrophy. Methods This Chinese family with Avellino corneal dystrophy were determined and surveyed in Peking University Third Hospital.Periphery blood from 8 patients with Avellino corneal dystrophy and 2 unaffected subjects were collected from a Chinese family with corneal dystrophy for the extraction of DNA.Exons 4,11,12 of the TGFBI gene were amplified by polymerase chain reaction(PCR),and the amplified products were sequenced directly and compared the gene sequence with that of TGFBI in GenBank.Written informed consent was obtained from each Subject prior to any medieal process. Results This family included 27 members of consecutive 4 generation.The hereditary pattern W88 in accordance with the autosomal dominant inheritance.Directly sequencing of 8 affected members revealed a G tO A transition at codon 124 (CGC to CAC),producing R124H mutation of TGFBI gene.Two synonymous single nucleotide polymorphism(SNP)of TGFBI gene occurred in the family.including a C to T transition at eodon 472(CTC to CTT)in 8 members,and a T to C transition at codon 540(TTT>TTC)in 9 members,which wag unrelated with disease. Conclusion R124H mutation of the TGFBI gene is found in this Chinese family with Avellino corneal dystrophy.
9.Study on the Biochemical Mechanism of Degrading Keratins by Streptomyces fradiae
Lin HUANG ; Zhi-Qiang XIONG ; Hua-Jing CAI ; Mei-Jin GUO ; Guo-Quan TU ;
Microbiology 1992;0(04):-
The biochemical mechanism of degrading keratins by S.fradiae var S-221 was primarily studied.The compounds (Na_ 2 SO_ 4 , Na_ 2 SO_ 3 and sulfdryl acohol), which respecitively enhance specific activity of keratinase, activate keratinase intensively and mainly act on the disulfide bonds reductase in the keratinase, Na_ 2 SO_ 3 activates intensively both disulfide bonds reductase and polypeptide hydrolytase at 0.01 mol/L, whereas Na_ 2 S_ 2 O_ 3 , which acts on the disulfide bonds reductase, inhibits keratinase.On the condition that substrate, keratins exists, S.fradiae var S-221 is induced to produce exo-keratinase, which is a multiproteinase, containing disulfide bonds reductase, which is a key enzyme degrading keratins, then, with polypeptidic, hydrolytase, graduately hydrolyzates denatured keratins into polypeptides, oligopeptides and free amino acids, so that keratins have been decomposed completely.Sulfur in the keratins was transferred into sulfhydryl compounds, H_ 2 S and sulfates in the course of keratinolysine.
10.PCR-SSCP Analysis of the Pronucleus Microbial Communities in Luzhou-flavor Daqu
Hui-Bo LUO ; Zhi-Guo HUANG ; Hao LI ; Qiang ZHANG ; Chun-Hui WEI ;
Microbiology 2008;0(09):-
PCR-SSCP(single-strand conformation polymorphism) was used for studying the community changes of pronucleus microorganisms in various fermenting stages of Luzhou-flavor Daqu. The results showed:(1) The pronucleus microorganism's community was similar and also had the polymorphism in each simple of various fermentation stage;(2) Different microflora had complex ecology effects of coordination and the restriction;(3) The diversity indexes of different stage of Daqu microorganisms were around 1.69~2.01,and the composition of them was stable relatively;(4) The similarity indexes were 0.67~1.00,and much higher in the approaching stages.