Objective To explore the variance of helicobacter pylori(Hp) infection rate in children with recurrent abdominal pain(RAP).Methods By using the()~(13)C-urea breath test(~(13)C-UBT) to detect the status of Hp infection in 1676 children with RAP from 1998 to 2004.Results 1.Total 1676 cases of children with RAP were detected.There were 438 cases showed Hp positive,the positive rate was 26.13%.The infection rates in patients from 2-5,5-10,and 10-14 years of age were 23.66%(84/355),(26.31%)(286/1087),29.06%(68/234),respectively.2.Hp infection rates in children with RAP from 1998 to 2004 were 29.33%(83/283),(28.90%)(76/263),25.76%(51/198),26.01%(56/215),25.81%(48/186),24.45%(67/274),22.18%(57/257),respectively.Conclusion 1.The Hp infection rate in children with RAP increased with age;2.The Hp infection rate in children with RAP decreased year after year.

Objective To analyze the allele frequencies of 5 short tandem repeat(STR)loci(D12S313,D12S304,D12S1640,D12S1708 and D12S1583)on chromosome 12 among Kashin-Beck disease(KBD)patients and the control population living in the area suffered from KBD.Methods Fifty KBD patient8 and 50 non-KBD patients were chosen in endemic afea of Shaanxi Province,5 STR loci on chromosome 12 were genotyped by the technology of polymerase chain reacfion(PCR)and capillary electmphoresis.The pelymorphisms of STR in these popIllations were analyzed.The allele and genotype frequencies of each STR in the corresponding groups were caleulated and compared. Results In KBD group,the 5 STR loci had 8,6,7,5 and 11 types ofalleles and 17,11,15,8 and 28 genotypes, respectively;while in the control group,the number of aUele types of 5 STR loci were 6,8,6,4 and 10,the number of genotype of those loci were 13,21,14,8 and 23,respectively The allele frequence of D12S304 locus was statiBtically significant between KBD patients and controls(P<0.05),especially for the 319 bp allele(P<0.006 25). Conclusion There is an association between D12S304 locus and KBD.The 319 bp allele might play the key role.

Objective: To study the chemical constituents from the root barks of Cudrania cochinchinensis. Methods: The chemical constituents were isolated and purified by silica gel column chromatography. The structures of the compounds were identified on the basis of spectral data (MS, 1H-NMR, 13C-NMR, and 2D NMR) and by the comparison of spectroscopic data with the reported values in the literatures. Results: A new xanthone, 1,6,7-trihydroxy-4-(1,1-dimethylallyl)-3-methoxyxanthone (1) and a known prenylated xanthone 1,5,6-trihydroxy-4-(1,1-dimethylallyl)-3-methoxyxanthone (isocudraniaxanthone B, 2) were isolated from the root barks of C. cochinchinensis. Conclusion: Compound 1 is a new prenylated xanthone. Isomers 1 and 2 are obtained from this plant for the first time. © 2013 Tianjin Press of Chinese Herbal Medicines.

Myopia is the most important cause of visual impairment in adolescents. However, its etiology is complex. In recent years, a large number of epidemiological studies have been done on risk factors of myopia. Most of these studies is cross- sectional study, not longitudinal cohort study. Overall, the incidence of myopia is the result of the interaction between genetic susceptibility and environmental exposure. This review is about the risk factors for myopia.

One of the strain of bivalent HFRS vaccine, Z37 strain was isolated from Rattus norvegicus and identified as SEO virus by serological test. The M segment cDNA of Hantavirus Z37 strain was obtained by reverse transcripti on and polymerase chain reaction, subsequently cloned into pGEM-T vector. The s equence of positive recombinants was determined by the method of dideoxy chain t ermination, which revealed that the M genomic segment is 3651 nucleotide in len gth with a predicated long open reading frame encoding a protein of 1133 amino acids. Comparison with HNT type (76-118, A9, HV-114 strains) indicated that th ere were 71.8%～72.1% homology at the nucleotide level, 76.2～76.7% homology at the amino acid level. Comparison with SEO type (R22,L99,80-39 strains) showed 95.3～96.1 homology at the nucleotide level, 95.3～99.2% homology at the amino acid level. The results of nucleotide and amino acid comparison indicated that Z 37 strain is SEO viruses in molecular level.

Hepatectomy ; methods ; Humans ; Liver ; blood supply

AIM: To investigate the effect of low intensity white light irradiation on the retinas of mice.
METHODS: Thirty C57BL/6J mice were randomly divided into two groups. The number of the mice in each group was 15. The mice in experimental group received dark adaptation from 5:00p. m. to 6:00p. m. , and then exposed to LED white light from 6:00p. m. to 7:00p. m. everyday for a month. At 1, 3, 7, 14 and 30d after the beginning, we examed the histology of mice retinas, calculated the thickness of outer nuclear layer ( ONL ) , inner nuclear layer ( INL ) and analyzed electrophysiology of mice.
RESULTS:One month after experiment, compared to the control group, the latency of Rod-R a wave of the mice in experimental group significantly prolonged, the amplitude of Cone-R b wave of the mice in experimental group significantly decreased and the latency of b wave of the mice in experimental group significantly prolonged ( P<0. 05). There are no significant difference in the histology of retina, ONL and INL thicknesses.
CONCLUSION: 100lux low intensity white light could give rise to the impairment of the retinal functions in dark-adapted mice.

Adult ; Bronchial Hyperreactivity ; physiopathology ; Bronchial Provocation Tests ; Chronic Disease ; Cough ; physiopathology ; Female ; Humans ; Male ; Middle Aged ; Respiratory Function Tests

Animals ; Beverages ; Macrophages ; drug effects ; metabolism ; Mice ; Mice, Inbred Strains ; Receptors, Complement 3b ; metabolism

In order to optimize extraction process conditions of tannins from Geranium orientali-tibeticum by supercritical CO2, the content of tannins was determined by phosphomolybdium tungsten acid-casein reaction, with extraction pressure, extraction temper- ature and extraction time as factors, the content of tannins from extract of G. orientali-tibeticum as index, technology conditions were optimized by orthogonal test. Optimum technology conditions were as follows: extraction pressure was 25 MPa, extraction temperature was 50 °C, extracted 1.5 h. The content of tannins in extract was 12.91 mg x g(-1), extract rate was 3.67%. The method established could be used for assay the contents of tannin in G. orientali-tibeticum. The circulated extraction was an effective extraction process that was stable and feasible, and that provides a way of the extraction process conditions of tannin from G. orientali-tibeticum.
Carbon Dioxide ; chemistry ; Chemical Fractionation ; methods ; Drugs, Chinese Herbal ; chemistry ; Geranium ; chemistry ; Tannins ; isolation & purification