Objective To learn the main pathogenic microorganisms,and to acquire the data of pathogens composition anddrug resistance of infant diarrhea disease in Wenzhou City.Methods The diarrheal stool specimens of <5 years oldchildren were collected from outpatients and inpatients in children′s hospital and were cultured and detected.Results Atotal of 201 strains pathogenic bacteria were separated from 51 7 clinical specimens,and the rate of total detection is38.88%,of which included 74 strains intestinal pathogenic bacteria and 1 27 strains diarrhea virus,with the rate of detection1 4.31 % and 24.56%,respectively.Among intestinal pathogenic bacteria,the E.coli was the most common bacteria(36strains,48.65%),followed by salmonella(31 strains,41 .89%).Diarrhea virus included norovirus type 2 (56 strains,44.09%),norovirus type 1 (24 strains,1 8.90%),A group rotavirus (56 strains,31 .50%)and goblet virus(7 strains,5.51 %).The resistance rate of Salmonella to ampicillin was 64.52%,and that to ampicillin,tetracycline,compound newMing were more than 70.00%.The resistance rate of Rifampicin.Novobiocin in all bacteria reached 1 00%.Conclusion E.coli and salmonella epidemic strains were the common pathogenic bacteria in infectious diarrhea among infants inWenzhou City.The predominant viruses were norovirus and rotavirus group A.The drug resistance of various pathogenicbacteria was different,and active surveillance should be strengthened.

Objective: To summarize the clinical characteristics of tumour necrosis factor receptor-associated periodic syndrome (TRAPS) in children. Methods: The clinical manifestations, laboratory tests, genetic testing and follow-up of 10 children with TRAPS from May 2011 to May 2021 in 6 hospitals in China were retrospectively analyzed. Results: Among the 10 patients with TRAPS, including 8 boys and 2 girls. The age of onset was 2 (1, 5) years, the age of diagnosis was (8±4) years, and the time from onset to diagnosis was 3 (1, 7) years. A total of 7 types of TNFRSF1A gene variants were detected, including 5 paternal variations, 1 maternal variation and 4 de novo variations. Six children had a family history of related diseases. Clinical manifestations included recurrent fever in 10 cases, rash in 4 cases, abdominal pain in 6 cases, joint involvement in 6 cases, periorbital edema in 1 case, and myalgia in 4 cases. Two patients had hematological system involvement. The erythrocyte sedimentation rate and C-reactive protein were significantly increased in 10 cases. All patients were negative for autoantibodies. In the course of treatment, 5 cases were treated with glucocorticoids, 7 cases with immunosuppressants, and 7 cases with biological agents. Conclusions: TRAPS is clinically characterized by recurrent fever accompanied by joint, gastrointestinal, skin, and muscle involvement. Inflammatory markers are elevated, and autoantibodies are mostly negative. Treatment mainly involves glucocorticoids, immunosuppressants, and biological agents.
Male ; Child ; Female ; Humans ; Child, Preschool ; Receptors, Tumor Necrosis Factor, Type I/genetics* ; Retrospective Studies ; Hereditary Autoinflammatory Diseases/drug therapy* ; Glucocorticoids/therapeutic use* ; Biological Factors/therapeutic use* ; Immunosuppressive Agents/therapeutic use* ; Autoantibodies ; Familial Mediterranean Fever/diagnosis* ; Mutation