Animals ; Apoptosis ; Carcinoma, Hepatocellular ; pathology ; virology ; Cell Line, Tumor ; Cell Proliferation ; Hepatitis B virus ; Hepatocytes ; pathology ; virology ; Humans ; Liver Neoplasms ; pathology ; virology ; Trans-Activators ; genetics ; metabolism ; physiology

Animals ; Apoptosis ; Cell Cycle ; Cell Cycle Proteins ; genetics ; metabolism ; physiology ; DNA Repair ; Homeodomain Proteins ; genetics ; metabolism ; physiology ; Humans ; Inhibitor of Growth Protein 1 ; Intracellular Signaling Peptides and Proteins ; genetics ; metabolism ; physiology ; Neoplasm Metastasis ; Neoplasms ; metabolism ; pathology ; Neovascularization, Pathologic ; pathology ; Nuclear Proteins ; genetics ; metabolism ; physiology ; Prognosis ; Receptors, Cytoplasmic and Nuclear ; genetics ; metabolism ; physiology ; Signal Transduction ; Transcription Factors ; genetics ; metabolism ; physiology ; Tumor Suppressor Protein p53 ; metabolism ; Tumor Suppressor Proteins ; chemistry ; genetics ; metabolism ; physiology

Prostate cancer (PCa) incidence is increasing in China.Because of the limited clinical symptoms,early detection of PCa is critical and difficult.Current clinical diagnostic methods for localizing PCa including both the conventional anatomic imaging and histological techniques have many problems.Enormous novel PCa specific molecular probes have been evaluated.Those probes have substantially improved the diagnostic accuracy and specificity of PCa.This review briefly introduces the conventional noninvasive imaging techniques for PCa diagnosis.Nuclear medicine probes that have great clinical value for PCa molecular imaging are also discussed.

Chimeric antigen receptor T-cell (CAR-T) therapy is an emerging cancer immunotherapy strategy in recent years. CAR-T therapy has shown significant efficacy in the treatment of relapsed or refractory diffuse large B cell lymphoma and refractory pediatric acute lymphoblastic leukemia. However, restricted by the complexity of solid tumor microenvironment, the application of CAR-T therapy in solid tumors is still underway. Molecular imaging can trace the in vivo status of T cells in real time and in depth, which is of great significance to investigate the biological behavior of CAR-T cells in vivo and to evaluate their activation status. Meanwhile, molecular imaging strategy can also act as an early predictor of CAR-T treatment efficacy and related side effects, which can provide effective information for clinical intervention and play an important role in guiding the development of CAR-T therapy.

Alternative Splicing ; Apoptosis ; Cell Cycle ; Cell Proliferation ; Cyclin-Dependent Kinase Inhibitor p21 ; metabolism ; Hep G2 Cells ; Humans ; Inhibitor of Growth Protein 1 ; Intracellular Signaling Peptides and Proteins ; genetics ; metabolism ; physiology ; Nuclear Proteins ; genetics ; metabolism ; physiology ; Plasmids ; Protein Isoforms ; Proto-Oncogene Proteins c-bcl-2 ; metabolism ; Recombinant Proteins ; genetics ; metabolism ; Transfection ; Tumor Suppressor Protein p53 ; metabolism ; Tumor Suppressor Proteins ; genetics ; metabolism ; physiology ; bcl-2-Associated X Protein ; metabolism

Hypoxiaisoneofthemostimportantfactorsinfluencingcancertherapyandclinicalprogno-sis.With positron emission tomography (PET)widely adopted in clinical practice,the development and appli-cation of PET hypoxia imaging agents has been much popular in the field currently.PET hypoxia imaging can detect tumor hypoxia region noninvasively,which has important significance for optimizing cancer treatment decisions and improving the prognosis of cancer.

The somatostatin analogue pasireotide is a new type of protein which is the first therapeutic agent targeted to the pituitary.Pasireotide can prevent adrenocorticotropic hormone release and inhibit the growth of tumor cells after coupling with somatostatin receptor of the target cell membranes.Pasireotide has a high binding affinity for most of somatostatin receptor (SSTR) subtypes and in particular for SSTR5.Pasireotide can paly an important role in the new round of new targets for individualized diagnosis and treatment of tumors through the studies of translational medicine.

Objective To label human VEGF targeted bevacizumab (avastin) with 111In and to evaluate the application of 111 In?DTPA?avastin SPECT imaging for tumor diagnosis. Methods DTPA?avastin was prepared by coupling with a bifunctional chelating agent, and then labeled with 111 In to obtain 111 In?DTPA?avastin. The stability and molecular integrity of the labeled radiotracer were studied. Human hepatoma cell ( BEL7404) bearing nude mice tumor model was employed for tumor targeting evaluation. Gamma imaging was acquired after intravenous injection of 18.5 MBq probe. At the end of the observation, animals were sac?rificed for bio?distribution study. Results 111 In?DTPA?avastin tracer was synthesized and purified to a?chieve a radiochemical purity yield above 98% and specific activity up to 185 GBq/nmol. Its stability in 5%BSA was optimal, and the radiochemical purity after incubation for 96 h was over 90%. Gamma imaging re?sults showed that the tracer possessed definite tumor targeting property. Its biodistribution was consistent with that of normal in vivo antibody metabolism while possessing a good tumor?targeting property with a relatively high uptake of (3.8±0.8) %ID/g in tumor tissues 96 h after injection. Conclusions 111 In?DTPA?avastin tracer has good physicochemical properties, in vivo stability and good VEGF targeted binding. 111 In?DTPA?avastin has potential to be a new molecular probe for SPECT imaging.

Gas Chromatography-Mass Spectrometry ; Solvents ; analysis ; chemistry ; Volatile Organic Compounds ; analysis ; chemistry

Air Pollutants, Occupational ; analysis ; Solvents ; analysis