1.Vitrectomy combined with inferior scleral buckling for a special kind of retinal detachment
Heng, LI ; Zhi-Gang, LIU ; Xue, MI
International Eye Science 2014;(8):1519-1521
AIM:To evaluate the efficiency of vitrectomy combined with inferior scleral buckling for a special kind of retinal detachment.
METHODS: Nineteen eyes of special retinal detachment had following features: 1 ) the course was more than 6mo;2) there were at least one hole located in the 5 : 00 - 7 : 00 of the marginal retina; 3 ) the detachment range of retina more than 270 degrees and retinal proliferation in retinal detachment. The 19 eyes who received vitrectomy combined with inferior scleral buckling, retinal anatomic reattachment and visual function recovery was observed.
RESULTS: Among these 19 eyes, all eyes retinal anatomic reattachment and best corrected visual acuity ( BCVA ) was improved in various degrees. The BCVA was 0. 01-0. 1 in 5 eyes, 0. 12-0. 3 in 9 eyes, and ≥0. 4 in 5 eyes.
CONCLUSION: It is an effective method to vitrectomy combined with inferior scleral buckling for the special kind of retinal detachment which has following features:1) the course was more than 6mo;2) there were at least one hole located in the 5:00-7:00 of the marginal retina;3 ) the detachment range of retina more than 270 degrees and retinal proliferation of cable in retinal detachment.
2.Primary mucosal melanoma of the nasal cavity: a linicopathological analysis of 17 cases
Wen LIU ; Zhi ZENG ; Heng ZHOU ; Hao WU ; Jingping YUAN
Chinese Journal of Clinical and Experimental Pathology 2017;33(2):169-173
Purpose To study the clinicopathologic features and differential diagnosis of primary mucosal melanoma of the nasal cavity (PMMNC).Methods 17 cases of PMMNC diagnosed from January 2003 to September 2016 were studied by clinical pathological analysis and immunohistochemical staining,and relevant literatures were reviewed.Results 73% of the PMMNC was characterized by unilateral nasal congestion and intermittent epistaxis and 61% of the PMMNC occurred in the nasal septum and nasal side wall.Microscopically,the organizational structure and morphology were complex and diverse,which had several cell types including epithelioid cell type (6cases,35.3%),spindle cell type (3 cases,17.6%) and snall cell type (5 cases,29.4%),the other 3 cases (17.6%)were mixed cell type.Mitotic activity and tumor necrosis were more likely to be seen in PMMNC,among other clinicopathological features with a small amount of fibrous stroma and melanoma and rich blood vessels.The immunohistochemical study showed that the positive rate of S-100 and HMB-45 were both 93.8%(15 cases) and those of Melan-A and vimentin were both 87.5% (14 cases),while CK and EMA were both negative (16 cases).Conclusion PMMNC is a rare disease and the phenotype of S-100,HMB-45,Melan-A and vimentin are useful for diagnosis of PMMNC.
3.Entry of antibiotic ointment into the anterior chamber following sutureless clear corneal incisions phacoemulsification:a case report
Sheng-Li, HAO ; Zhi-Heng, LIU ; Zhi-Guo, GAO ; Guo-Zhi, XU
International Eye Science 2011;11(8):1318-1320
·AIM:To report a case of entry of antibiotic ointment into the anterior chamber following sutureless clear corneal incisions phacoemulsification.·METHODS:A 62-year-old man had uneventful phaco-emulsification with clear corneal incisions with intraocular lens(IOL) implantation. Postoperative medication included antibiotic/steroid ointment; the eye was firmly patched at the end of the procedure. On the first postoperative day, the patient was seen an oily droplet floating in the anterior chamber. Immediate surgical removal of the ointment without IOL exchange was performed.·RESULTS:During follow-up for six months after irrigation/aspiration of the anterior chamber and removal of the oily droplet, visual acuity and intraocular pressure (IOP) were unchanged and anterior segment was formed and quiet.·CONCLUSION:Ophthalmic ointment may ingress into the anterior chamber through unstable clear corneal incisions. The case highlights the importance of appropriate wound construction and integrity, as well as the risk of tight eye patching following placement of ointment.
4.Histologic classification of nasopharyngeal carcinoma.
Kuang-rong WEI ; Ying XU ; Wen-jun ZHANG ; Zhi-heng LIANG ; Jing LIU
Chinese Journal of Pathology 2011;40(5):355-357
5.Relation between iodine intake and thyroid goiter.
Chinese Journal of Epidemiology 2004;25(8):726-728
China
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epidemiology
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Female
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Goiter
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chemically induced
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epidemiology
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Humans
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Iodine
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administration & dosage
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urine
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Male
6.Development and evaluation of complex intervention
Miao LIU ; Zhi-Heng HONG ; Si-Yan ZHAN
Chinese Journal of Epidemiology 2010;31(12):1410-1413
Experimental studies with only one intervention are not always sufficient for health care issnes, while the method of complex intervention is more useful. But the complex intervention is more complicated and needs carefully design and evaluation. This article will introduce the framework for design and evaluation of complex intervention and the revised version made by Medical Research Council, with five examples showing its application.
7.ISOLATION OF ACIDOPHILIC AND ACIDODURIC STREPTOMYCETES USING DISPERSION AND DIFFERENTIAL CENTRIFUGATION APPROACH
Li-Ming WANG ; Ying HUANG ; Qing-Feng CUI ; Qiong XIE ; Ya-Mei ZHANG ; Zhi-Heng LIU ;
Microbiology 1992;0(06):-
Technological improvement for microorgnism isolation is important since isolation provides substantial materials for the exploitation of new microbial resources. In this study, a new approach, dispersion and differential cetrifugation (DDC), was applied in the isolation of acidophilic and acidoduric streptomycetes from 12 acid soil samples. Contrast with traditional method, the new approach yielded satisfying results with 2 - 20 times isolation efficiency and good selectivity. 45 representatives out of 249 streptomycetes isolates, which belonged to 12 color groups, showed morphology and cell wall type consistent with streptomycetes. The optimum pH range for their growth were between pH 4.5 - 5.5. It is proved that we succeeded in the rare-streptomycetes isolation using DDC approach.
8.Prostate cancer cell vaccine transfected with 4-1BBL induces anti-tumor immunity in vitro.
You-lin KUANG ; Xiao-dong WENG ; Xiu-heng LIU ; Zhi-yuan CHEN ; Heng-cheng ZHU ; Bo-tao JIANG
National Journal of Andrology 2010;16(9):773-777
OBJECTIVETo explore the anti-tumor immunity in vitro induced by prostate cancer cell vaccine transfected with recombinant adenovirus encoding 4-1BBL in mice.
METHODSThe replication-deficient adenovirus AdEasy-1 system was used to construct recombinant adenovirus Ad-m4-1BBL and Ad-eGFP. The prostate cancer cell RM-1 of mice was transfected with Ad-m4-1BBL and Ad-eGFP, and treated with mitomycin (MMC) to produce TCV, TCV-Ad-eGFP and TCV-Ad-m4-1BBL, followed by co-culture with syngeneic murine spleen cells. Then the cytotoxic activity of the lymphocytes against RM-1 cells was analyzed with CCK-8 solution, and IL-2 and INF-gamma were detected by ELISA.
RESULTSThe 4-1BBL protein was highly expressed in the TCV-Ad-m4-1BBL of the 4-1BBL-transfected mice. TCV-Ad-m4-1BBL significantly increased the expressions of IL-2 ([180.24 +/- 2.22] pg/ml) and INF-gamma ([1512.46 +/- 23.64] pg/ml) as compared with TCV and TCV-Ad-eGFP (P < 0.05), and induced higher RM-1 cell specific cytotoxicity ([34.24 +/- 2.64]%) than the latter two ([9.82 +/- 1.48]%) and ([14.65 +/- 3. 21]%), (P < 0.05). But none of them exhibited significant cytotoxicity against hepatocellular carcinoma Hepal-6.
CONCLUSIONThe m4-1BBL-expressing prostate cancer cell vaccine can effectively induce anti-tumor immune responses.
4-1BB Ligand ; genetics ; immunology ; Animals ; Cancer Vaccines ; genetics ; immunology ; Cell Line, Tumor ; Coculture Techniques ; Cytotoxicity, Immunologic ; genetics ; Female ; Interleukin-2 ; metabolism ; Male ; Mice ; Mice, Inbred C57BL ; Prostatic Neoplasms ; Transfection
9.Effects of tetramethylpyrazine on cytosolic free calcium concentration in penis corpus cavernsum smooth muscle cells in rabbits.
Zhi CHEN ; Ji-Hong LIU ; Chun-Ping YIN ; Jun CHEN ; Heng-Jun XIAO
National Journal of Andrology 2003;9(5):331-334
OBJECTIVETo study the effects of Chinese medicine tetramethylpyrazine (TMP) on intracellular free calcium ([Ca2+]i) in cultured penis corpus cavernosum smooth muscle cell (PCSMC) in rabbits.
METHODSBy using laser scanning confocal microscope (LSCM), the [Ca2+]i fluorescence signal changes was investigated in cultured PCSMC loaded with Ca2+ indicator Fluo-3/AM and divided into potassium chloride(KCl) group and norepinephrine (NE) group. Compared with verapamil (Ver), the effects of TMP was observed in different concentrations on [Ca2+]i increase induced by high potassium and NE.
RESULTSTMP had no obvious effect on resting PCSMC [Ca2+]i. It was found that 1, 10, 100 mumol/L TMP significantly inhibited [Ca2+]i increase induced by high potassium-depolarization. The peak inhibition rates were (38.6 +/- 3.0)%, (44.1 +/- 2.4)% and (53.7 +/- 4.1)% respectively. TMP could also inhibit cytosolic calcium pool release induced by 1 mumol/L NE. The peak inhibition rates were (13.9 +/- 2.7)%, (21.2 +/- 1.9)% and (29.5 +/- 3.6)% respectively.
CONCLUSIONSTMP can inhibit rabbit PCSMC [Ca2+]i significantly by suppressing voltage-dependent calcium channel and cytosolic calcium pool release. The effect, similar to Ver, signifies the important mechanism of erectile dysfunction (ED) therapy.
Animals ; Calcium ; metabolism ; Cells, Cultured ; Dose-Response Relationship, Drug ; Male ; Muscle, Smooth, Vascular ; cytology ; metabolism ; Penis ; blood supply ; metabolism ; Pyrazines ; pharmacology ; Rabbits ; Vasodilator Agents ; pharmacology
10.Study on scavenging activity to DPPH free radical of different polarity components in Guizhou Miao medicine "bod zangd dak".
Hong-zhi DU ; Heng NONG ; Li-sha DONG ; Jia-li LI ; Ming LIU ; Xi-cheng HE ; Jing ZHANG
China Journal of Chinese Materia Medica 2015;40(12):2449-2454
The paper is aimed to search more natural plant antioxidants and further research and develop new medicinal plant resources in Guizhou. The Guizhou special miao medicine "bod zangd dak" was extracted with 60% ethanol. The antioxidant activity of the different polarity components separated from the extract was tested by DPPH method with ascorbic acid as positive control. The results showed that the IC50 of the different polarity components was as following: ascorbic acid (0.033 4 g x L(-1)) < ethyl acetate components (0.052 3 g x L(-1)) < total tannins components (0.054 9 g x L(-1)) < 60% ethanol extraction components (0.076 7 g x L(-1)) < butanol extraction components (0.110 g x L(-1)) < water-soluble polysaccharides components (0.168 g x L(-1)) < water extraction components (0.174 g x L(-1)) < water components after extraction (0.226 g x L(-1)) < total polysaccharides components (0.645 g x L(-1)). It is concluded that the different polarity components have different free radical scavenging activity and that provides a scientific basis for further search of the active ingredients and the activive mechanism.
Antioxidants
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chemistry
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isolation & purification
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Biphenyl Compounds
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chemistry
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China
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ethnology
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Drugs, Chinese Herbal
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chemistry
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isolation & purification
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Free Radicals
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chemistry
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Medicine, Chinese Traditional
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Picrates
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chemistry
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Plant Roots
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chemistry
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Plant Stems
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chemistry
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Smilax
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chemistry