Objective To investigate the clinical characteristics of colorectal cancer and its trend in Guangdong province.Methods The data from 6501 patients with pathologically confirmed colorectal cancer of 5 hospitals from 1990 to 2004 was analyzed.Results The mean age of 6501 patients with colo- rectal cancer was 59 years old,with the predominant age ranged from 61 to 70 years old.The proportion of young patients decreased from 7.1% to 3.5%.and the ratio between male and female was 1.47:1, which was gradually increased with increasing of age.The prevelence reached a high peak(60 years old) and dropped after that as a single peak curve.The ratio between rectal cancer and colon cancer was 1.1: 1,and the descending colon cancer accounted for 27.6%.With ageing,the rectal cancers decreased while the right hemi colon cancer increased gradually.Tubular adenocarinoma was predominant with his- tological typing,which accounted for 76.0%.The progressing stage(Dukes B,C,and D)was found in 93.9% of all cases.Conclusions The onset age of patients with colorectal cancer become close to west- ern country.The proportion of young patients are decreasing.The ratio between male and female dis- plays a single peak curve from rising to dropping.A tendency of decreasing percentage in rectal cancer and the gradual increasing in right hemi colon cancer emphasize the importance of colonoscopy in colorec- tal caner screening.

Objective:To observe the effect of “bulk-fill”flowable resin in filling deep wedge-shaped defects.Methods:50 pa-tients with totally 85 pairs of premolars with deep wedge-shaped defects(depth >2 mm)were included.Each pair of the defects was randomly divided into the test group and the control group,the defects were treated by “bulk-fill”flowable resin with single cure tech-nique and conventional flowable resin with incremental layering technique respectively,the tooth sensitive rate 1 week after filling,the filling failure rate,the edge coloring and secondary caries rate 2 years after filling of the 2 groups were compared.Results:There was no significant difference between the 2 groups in the sensitive rate 1 week after filling,the failure rate and the edge coloring and sec-ondary caries rate 2 years after filling(P >0.05).Conclusion:The “bulk-filled”flowable resin using single cure technique can ac-quire the same clinical effect as conventional flowable resin using incremental layering technique in filling deep wedge-shaped defects.

Objective To explore the effect of HgCl2 exposure on energy metabolism of renal mitochondria in rats and the preventive effect of N-acetylcysteine(NAC).Methods Twenty-four Wistar rats were randomly divided into control group,HgCl2 group and NAC+HgCl2 group by body weight,8 in each.Control group and HgCl2 group were treated with physiological saline through intraperitoneal injection,NAC+HgCl2 group rats were intraperitoneally pretreated with NAC(0.4 mmol/kg),two hours after treatment,HgCl2 group and NAC+HgCl2 group were injected subcutaneously with HgCl2(1.84 ?mol/kg),but control group was injected with physiological saline,the volume of was 5 ml/kg,once a day for 14 consecutive days.Twenty-four hours after the last injection,all rats were sacrificed and the cortices of rat kidneys were collected.The mitochondria were prepared by using the differential centrifugation.The levels of malondialdehyde(MDA),the activities of phospholipase A2(PLA2) and succinate dehydrogenase(SDH),the mitochondrial membrane potential were measured.Results Compared with control group,the level of MDA and the activity of PLA2 significantly increased in HgCl2 group(P

Aim To investigate the effect of procyanidins on apoptosis in brain of experimental rats with intracerebral hemorrhage(ICH) and its possible mechanisms.Method Rat intracerebral hemorrhage model was established by injecting collagenase into the brain caudate nucleus.Fifty-four health Sprague-Dawley rats were divided into six groups randomly: sham operation group,model group of intracerebral hemorrhage,treatment groups with procyanidins at doses of 50 mg?kg~-1,100 mg?kg~-1 and 200 mg?kg~-1,and positive control group with nimodipine at dose of 10 mg?kg~-1.Twentyfour hours after operation,the content of glutathione(GSH) and activity of catalase(CAT) were measured.The levels of bax and bcl-2 were detected by method of immunohistochemistry,and the apoptosis in brain tissues was detected by method of TUNEL.Result In procyanidins groups(50,100 and 200 mg?kg~-1),the content of GSH and the activity of CAT were significantly higher than those in ICH group(P

OBJECTIVEThis study aimed to evaluate the success and survival rate of endodontically treated premolars restored by ceramic onlays by comparing restored by quartz fiber posts and metal ceramic crowns.

METHODSNinety-four patients with 126 endodontically treated premolars were enrolled in this study and divided into 4 groups according to the remaining axial walls and restorative methods. The observation time was 36 months. The success and survival rates of the restorations, as well as causes of failures, were analyzed.

RESULTSAt the final follow-up, the success and survival rates of the mild defect endodontically treated premolars were restored by quartz fiber posts and the crowns were at 96.3% and 98.1%; the success and survival rates of the severe defect premolars restored by quartz fiber posts and crowns were at 88.5% and 96.2%. The success and survival rates of the mild defect premolars restored by cast ceramic onlays were both at 96.6%, the success and survival rates of the severe defect premolars restored by cast ceramic onlays were at 94.1% and 100%, respectively. The success and survival rates of the different groups were no significant difference (P>0.05).

CONCLUSIONBased on the results and within the limits of this study, cast ceramic onlays is a very reliable method to restore endodontically treated premolars.

·AIM:To report a case of entry of antibiotic ointment into the anterior chamber following sutureless clear corneal incisions phacoemulsification.·METHODS:A 62-year-old man had uneventful phaco-emulsification with clear corneal incisions with intraocular lens(IOL) implantation. Postoperative medication included antibiotic/steroid ointment; the eye was firmly patched at the end of the procedure. On the first postoperative day, the patient was seen an oily droplet floating in the anterior chamber. Immediate surgical removal of the ointment without IOL exchange was performed.·RESULTS:During follow-up for six months after irrigation/aspiration of the anterior chamber and removal of the oily droplet, visual acuity and intraocular pressure (IOP) were unchanged and anterior segment was formed and quiet.·CONCLUSION:Ophthalmic ointment may ingress into the anterior chamber through unstable clear corneal incisions. The case highlights the importance of appropriate wound construction and integrity, as well as the risk of tight eye patching following placement of ointment.

Objecfive To establish the quality standard of Zaodi Anshen Pills. Methods TLC method was used to identify Spinosae and Radix polygoni Multiflori. HPLC method was used to determine the schisandrin, deoxyschizandrin and schisandrin B. Eclipse XDB-C18 column (250 mm×4.6 mm, 5 μm) was adopted by gradient elution using MeOH-water solution as the mobile phase. The detection wavelength was at 254 nm, column temperature was at 40 ℃ and the flow rate was 1.0 mL/min. Results The TLC spots were clear without interference of the negative control. The linear ranges of schisandrin, deoxyschizandrin and schisandrin B were within 338-1690 ng (r 2=0.999 1), 128-640 ng (r 2=0.999 3) and 236-1180 ng (r 2=0.999 4), and the average recoveries were 99.2%, 99.0% and 98.9% respectively. Conclusion This method is accurate, reliable, specific and reproducible, and can be used for quality control of Zaodi Anshen Pills.

Objective To explore the blood drug concentration monitoring of sustained-release valproate(DK)in children with epilepsy,focusing on the selection of sampling time and evaluation of the results.Methods Two hundred and seventy-one children taking DK and 155 children taking sodium valproate syrup(VPA Syr)were involved and their serum were taken when achieved steady state to determine the valproic acid level using fluorescence polarization immunoassay.They were divided into 4 groups,which were DK taken once daily group(DK qd group,126 children),DK taken once daily at night and sampled on morning group(DK qn group,26 children),DK taken every 12 h group(DK q12 h group,119 children),VPA Syr q12 h group(155 children).Determine the proportion of the blood drug concentration of each group below,ithin and above the therapeutic range for valproate(50-100 mg/L)were determined.The data were analyzed by t test.Results The Cmin of DK qd group were(73.09?19.91)mg/L,significantly lower from the serum concentration of DK qn and sampled on morning group [(94.94?25.44)mg/L](P0.05).Conclusions DK qn should sampled at night before the night dose.The Cmin of DK q12 h was higher according to the therapeutic range,it's favorable range still needs clinical practice.

Congresses as Topic ; Otolaryngology

BackgroundOsteopontin is highly expressed in many metastatic tumors,but its expression in uveal melanoma and the relationship of osteopontin with metastasis is still under investigation.Objective This study was to investigate the relationship between osteopontin expression and uveal melanoma metastatic potential.Methods Fifty samples of uveal melanoma were collected in Beijing Tongren Hospital.The expressions of osteopontin in the samples were detected using immunohistochemistry,and expressions of osteopontin mRNA in different metastatic potential uveal melanoma cell lines (MU M-2B,C918,OCM-1A) were analyzed using quantitative polymerase chain reaction (Q-PCR).ResultsOsteopontin was positively expressed in 10 samples of 13 patients with metastasis and 14 samples of 37 patients without metastasis,showing a significant difference between them (x2=5.888,P=0.000).There were 11 samples and 13 samples exhibiting the positive expression of osteopontin in 15 epithelial type and 35 non-epithelial type of uveal melanoma respectively with the significant difference (x2=5.510,P =0.000).The positive expressing numbers of osteopontin were 20 tumors of 30 affected tumors and 4 tumors of 20unaffected ciliary tumors with the significant difference (x2 =10.470,P =0.000 ).No significant differences in the expression of osteopontige were found between different gender,age,eyes,largest basal diameters and invasion of scleral canal( P =0.536,0.256,0.802,0.848,0.555 ).The relative expression value of osteopontin mRNA was in turn 1.00±0.04,0.91 ±0.03,0.08±0.01 in MUM-2B,C918,OCM-1A with the significant difference among three cell lines( F=33.135,P＜0.05).The osteopontige mRNA expression was significant enhanced in high metastatic potentialcell lines( MUM-2B,C918 ) compared with low metastatic potential cell lines( OCM-1 A) (P＜0.05 ),but no significant difference was seen between two high metastatic potential cell lines(MUM-2B,C918) (P=0.804).Conclusions Osteopontige expression is associated with the uveal melanoma cell line invasion and metastasis potential.These results imply that osteopontige expression maybe an indicator of uveal melanoma invasion and metastasis ability.