1.Treatment of Pilon fracture with internal fixative operation with special-shaped plate
Zhi-Gang LIU ; Yan REN ;
Chinese Journal of Primary Medicine and Pharmacy 2006;0(08):-
Objective To investigate the effect of internal fixative operation with special-shaped plate on Pi- lon fracture.Methods 84 cases of Pilon fracture were treated respectively by continuous skeletal traction of calca- neus,internal fixative operation with general steel plate or Kirschner wire,or internal fixative operation with special- shaped plate.Results All the cases were followed up for 6 months to 2 years postoperatively.The rate of being ex- cellent and fine of 3 groups treated by 3 different methods was 42%,61% and 72 % separately.Conclusion Inter- nal fixative operation with special-shaped plate is a good way to treat Pilon fracture for its less complication and more recuperation and retention of function for anklebone.
3.Mangiferin protects rats against chronic bronchitis via regulating NF-kappaB (P65) and IkappaBalpha expression in mononuclear cells.
Zhi-Quan WEI ; Li YAN ; Jia-Gang DENG ; Jing DENG
Acta Pharmaceutica Sinica 2014;49(5):596-601
This study is to investigate the protective effect of mangiferin on NF-kappaB (P65) and IkappaBalpha expression in peripheral blood mononuclear cell (PBMC) in rats with cigarette smoke induced chronic bronchitis. The rat model with chronic bronchitis was established by cigarette smoke. Real-time fluorescence RT-PCR was executed for evaluating the NF-kappaB (P65) and IKkappaBalpha gene expression in mononuclear cell, and flow cytometry for their protein expression. The serum hs-CRP (high-sensitivity C-reactive proteins) and TNF-alpha (tumor necrosis factor-alpha) were detected by enzyme-linked immunosorbent assay. The histopathological score was obtained from lung tissue HE staining slides of lung tissue. The results showed that mangiferin could markedly suppress the NF-kappaB (P65) mRNA and protein expression in mononuclear cell, while promote the IkappaBalpha mRNA and protein expression. Furthermore, mangiferin could lower serum hs-CRP and TNF-alpha level, and reduce the chronic inflammatory damage of bronchiole. These results suggested that mangiferin could notably ameliorate chronic bronchiole inflammation induced by cigarette smoke, and this protective effect might be linked to the regulation of NF-kappaB (P65) and IkappaBalpha expression in mononuclear cell.
Animals
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Bronchi
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pathology
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Bronchitis, Chronic
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blood
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etiology
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metabolism
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pathology
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C-Reactive Protein
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metabolism
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I-kappa B Kinase
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genetics
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metabolism
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Leukocytes, Mononuclear
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metabolism
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pathology
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Male
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Mangifera
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chemistry
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Plants, Medicinal
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chemistry
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RNA, Messenger
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metabolism
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Random Allocation
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Rats
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Rats, Sprague-Dawley
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Tobacco Smoke Pollution
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Transcription Factor RelA
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genetics
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metabolism
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Tumor Necrosis Factor-alpha
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blood
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Xanthones
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isolation & purification
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pharmacology
5.Effects of glycyrrhizic acid and solid dispersions of curcumin on anti-oxidation and components of lung lavage fluid of SiO2 exposed rats.
Gang HAN ; Lin-Qi YAN ; Zhi-Yuan CHEN
Chinese Journal of Industrial Hygiene and Occupational Diseases 2010;28(4):289-290
Animals
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Bronchoalveolar Lavage Fluid
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chemistry
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Curcumin
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pharmacology
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Glycyrrhizic Acid
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pharmacology
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Lung
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drug effects
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metabolism
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Male
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Rats
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Rats, Wistar
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Silicon Dioxide
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toxicity
6.Outcomes of T3a Prostate Cancer with Unfavorable Prognostic Factors Treated with Brachytherapy Combined with External Radiotherapy and Hormone Therapy.
Zhi-peng MAI ; Wei-gang YAN ; Han-zhong LI ; Yi ZHOU ; Zhi-en ZHOU
Chinese Medical Sciences Journal 2015;30(3):143-149
OBJECTIVETo evaluate the outcomes of T3a prostate cancer with unfavorable prognostic factors treated with permanent interstitial brachytherapy combined with external radiotherapy and hormone therapy.
METHODSFrom January 2003 to December 2008, 38 patients classified as T3a prostate cancer with unfavorable prognostic factors were treated with trimodality therapy (brachytherapy + external radiotherapy + hormone therapy). The prescription dose of brachytherapy and external radiotherapy were 110 Gy and 45 Gy, respectively. The duration of hormone therapy was 2-3 years. The endpoints of this study included biochemical failure-free survival (BFFS), distant metastasis-free survival (DMFS), cancer-specific survival (CSS), and overall survival (OS). Survival curves were calculated using the Kaplan-Meier method. The Log-rank test was used to identify the prognostic predictors for univariate analysis.
RESULTSThe median follow-up was 71 months. The serum pre-treatment prostate-specific antigen (PSA) level ranged from 10.0 to 99.8 ng/ml (mean 56.3 ng/ml), the Gleason score ranged from 5 to 9 (median 8), and the percentage of positive biopsy cores ranged from 10% to 100% (mean 65%). The 5-year BFFS, DMFS, CSS, and OS rates were 44%, 69%, 82%, and 76%, respectively. All biochemical failures occurred within 40 months. The percentage of positive biopsy cores was significantly correlated with BFFS, DMFS, and OS (all P=0.000), and the Gleason score with DMFS (P=0.000) and OS (P=0.001).
CONCLUSIONST3a prostate cancer with unfavorable prognostic factors presents not so optimistic outcome. Hormone therapy should be applied to prolong the biochemical progression-free or metastasis-free survival. The percentage of positive biopsy cores and the Gleason score are significant prognostic factors.
Androgen Antagonists ; therapeutic use ; Brachytherapy ; Combined Modality Therapy ; Gonadotropin-Releasing Hormone ; agonists ; Humans ; Male ; Neoplasm Grading ; Prognosis ; Prostatic Neoplasms ; mortality ; pathology ; therapy ; Treatment Outcome
7.Expressions of receptor tyrosine kinases mRNA and protein in carcinoma of bladder.
Jin WEN ; Han-zhong LI ; Zhi-gang JI ; Wei-gang YAN ; Bing-bing SHI
Acta Academiae Medicinae Sinicae 2011;33(4):393-396
OBJECTIVETo detect the expressions of receptor tyrosine kinases (RTKs) mRNA and protein and to explore potentially promising tumor markers and conceivable drug target in bladder cancer.
METHODSThe expressions of RTKs mRNA and protein in tissue from invasive urothelial carcinoma of the bladder were examined by real-time quantitative PCR array and cytokine antibody array, with normal bladder tissue as control. The Results were analyzed using bioinformatic approaches.
RESULTSThe expressions of TGFA, STAB1, SERPINE1, ANGPT2, SPINK5, ANGPTL1, PROK1, MDK, CXCL9, GRN, RUNX1, VEGFA, and TGFB1 were obviously upregulated in bladder cancer tissue, while those of EDIL3, PTN, CCL2, PDGFD, FGF13, KITLG, FGF2, SERPINF1, and TNF were downregulated. ALK, Btk, EphB2, ErbB4, PDGFR-α, ROS, Tie-2, Tyk2, and VEGFR3 were over-expressed in bladder cancer, while FRK, Fyn, IGF-IR, Insulin R, Itk, JAK1, JAK3, and LCK were low-expressed.
CONCLUSIONVascular endothelial growth factor/platelet-derived growth factor-targeted therapies may play an active role in treating carcinoma of bladder.
Carcinoma, Transitional Cell ; metabolism ; Humans ; RNA, Messenger ; genetics ; Receptor Protein-Tyrosine Kinases ; genetics ; metabolism ; Urinary Bladder Neoplasms ; metabolism
8.Effects of plant growth regulator uniconazole on plant morphology and biomass allocation of Salvia miltiorrhiza.
Shu-rui GAO ; Zhi-gang ZHAO ; Jun-ling HOU ; Wen-quan WANG ; Yan SONG ; Bin-bin YAN ; Yan-qing JIN
China Journal of Chinese Materia Medica 2015;40(10):1925-1929
In this study, we use pot experiment to evaluate the effect of plant growth regulator on plant morphology and biomass allocation of Salvia miltiorrhiza. Different concentrations of uniconazole were supplied to S. miltioohiza by means of foliar spray. Height, breadth and stem diameter were measured dynamically, the biomass of leaf, stem, flower and fruit, root biomass and biomass ratio were also examined at the harvest time. Owing to the treatment, plant morphology showed significant changes, the height had been greatly reduced and the breadth decreased largely. Meanwhile, the biomass allocation changed too. The biomass ratio of leaf and stem had been notably reduced while the biomass ratio of root had been increased remarkably. It appears that foliar application of uniconazole during vigorous growth period in S. miltioohiza has dramatic effect on dwarfing plant and improving resistant to lodging. This measure could also be applied to condensed cultivation of S. miltioohiza to increase production.
Biomass
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Plant Growth Regulators
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pharmacology
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Plant Leaves
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drug effects
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growth & development
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Plant Roots
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drug effects
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growth & development
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Plant Stems
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drug effects
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growth & development
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Salvia miltiorrhiza
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drug effects
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growth & development
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Triazoles
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pharmacology
9.Implication of Increased serum uric acid level In patients with acute coronary artery syndrome
Yan-Gang WANG ; Feng XU ; Xin-Yan CHEN ; Sheng-Li YAN ; Shang-Lang CAI ; Zhi-Min MIAO
Chinese Journal of Endocrinology and Metabolism 2000;0(06):-
Acute coronary artery syndrome (ACS) was confirmed by coronary arteriography in 398 patients,and 378 healthy persons served as the control group.Serum uric acid in ACS and control group showed normal distribution,and serum uric acid level in ACS group (322?107 )?mol/L was significantly higher than those in control group (302?77)?mol/L (P<0.01 ).The prevalence of hyperuricemia (HUA) was 27.0% in male patients and 25.2% in female.Systolic blood pressure,diastolic blood pressure,fasting plasma glucose,total cholesterol,triglycerides,creatinine and blood urea nitrogen in ACS group were higher than those in control group (all P<0.01 ).Muhivariable analyses adjusted for age and sex indicated that raised fasting plasma glucose,total cholesterol,uric acid and mean arterial pressure were risk factors for coronary artery stenosis in ACS,and HUA played a role in the pathogenesis of ACS.
10.Research progress on trehalose used in lyophilization of blood cells--review.
Journal of Experimental Hematology 2006;14(2):416-418
Lyophilization is the best method for preservation of blood cells at present. Lyophilized blood cells could be stored at room temperature for long periods of time, while maintaining a high degree of viability. Lyophilized blood cells facilitates transportation and the costs are low. However, the membrane of blood cells is damaged and viability of blood cells is decreased in lyophilization. Trehalose has been shown to protect membrane, proteins and nucleic acids during freezing and desiccation. Now, researchers present a methor for loading blood cells with trehalose. In this paper, damage effect of lyophilization on blood cells, the mechanism of trehalose protection and the experimental studies on trehalose were reviewed.
Blood Preservation
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methods
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Cryopreservation
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methods
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Cryoprotective Agents
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pharmacology
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Erythrocyte Membrane
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metabolism
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Erythrocytes
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drug effects
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Freeze Drying
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methods
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Humans
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Trehalose
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pharmacology