Cell Proliferation ; Child ; Humans ; Nervous System Diseases ; surgery ; Neurons ; transplantation ; Stem Cell Transplantation ; adverse effects ; methods ; trends

China ; Intensive Care Units, Neonatal ; organization & administration

Child ; China ; Critical Illness ; Humans ; Intensive Care Units, Pediatric ; Pediatrics

China ; Humans ; Pediatrics ; trends

Female ; Humans ; Infant, Newborn ; Infant, Newborn, Diseases ; etiology ; genetics ; Maternal-Fetal Relations

Acute Kidney Injury ; etiology ; pathology ; therapy ; Anti-Bacterial Agents ; administration & dosage ; therapeutic use ; Anticoagulants ; administration & dosage ; therapeutic use ; Child ; Child, Preschool ; Consensus ; Female ; Hemofiltration ; instrumentation ; methods ; Humans ; Infant ; Intensive Care Units, Pediatric ; Male ; Multiple Organ Failure ; etiology ; pathology ; therapy ; Practice Guidelines as Topic ; Sepsis ; complications ; pathology ; therapy ; Severity of Illness Index

Infant neurodevelopmental assessment is a basic method of estimated high risk infant early development outcome.The early individualized neurodevelopmental assessment consist of monthly observations and evaluations of the infants' behavior modulation,motor repertoire which includes non functional and functional motor,autonomic system stability and muscle tensility,etc.The aims of early neurodevelopmental assessment are to discover the brain developmental abnormal and brain injure induced by various risk factors.Basis of the characteristics of which early brain development have better plasticity,administed early intervention and training,which can intervent neurodevelopmental problems such as cerebral palsy and developmental delay.By developmental assessment also make infant' parents to understand their children' ability and mental specialty,being help for infants' nursing,caring and early education.The applied of neurodevelopmental assessment can provide a new way and idea for infant' early development and intervention.

Objective To investigate the metabolic rules of surfactant protein A and D(SP- A,SP- D )in bronchoalveolar lavage fluid(BALF)in human fetal lungs during gestational ages. Methods BALF with 30 mL saline was performed on clinically collected human fetus with induction of labor by water- bag Their BALF was respectively retrieved [total retrieval rate(85. 6% ? 13 1)% ]for analysis of protein content. The BALF SP - A and SP - D from fetus of various gestational ages or newboms were detected by RPHA and ELISA. Results The total protein in BALF gradually increased since 10th week to newborn peak during lung development. And SP - A and SP D were respectively updated from(0.34 ?0.07 ) ,(0.05?0.01) ng/L to newborn climax[ (6 42 ? 0 36),(1.22 ? 0 13)ng/L] .Conclusions The protein in BALF gradually increases with fetal growth and lung development. SP-A and SP- D may reach prenatal climax and become the main indicator of newborn lung maturity.

Objective To observe the changes of neural stem cells(NSCs) in subventricular in developing human fetal brain at(diffe)-rent ages.Methods Thirty cases of embryoes at gestational age 24-28 weeks induced by labor with water bag were collected to determin distribution,shapes and growth modes in subventricular with hybridization in situ under light microscope.Results NSCs expressing Nestin mRNA existed in subventricular from human fetal brain at different ages,NSCs existed in subventricular of different fetal age included astro-NSCs,each had enations from 3 to 6,and all projections crowded together into a reticular plexiform,in which NSCs districuted.Nucli were round in shape,each had nucleoli from 1 to 4.NSCs had rarefaction chromatin,most NSCs existed in a single growth mode,symmetral cleavage growth mode and clony with 3 NSCs would be seen.There were no differences between positive Nestin mRNA NSCs in distribution and cell shapes,but had differences in growth mode.Conclusion NSCs exist in subventricular from different gestational ages and their growth mode are changing with difference of gestational age.