Human herpes simplex virus 1 (HSV-1) causes facial,ocular,and encephalitic disease and is associated with latent infection and cancer.Here,we developed a means of studying the pathogenesis of HSV-1 infection at the protein level by using the SELDI Protein Chip to detect changes of protein expression in Vero cells cultured in vitro.After infection with HSV-1 and culture for 12,24 or 48 h,cells were harvested and lysed.IMAC3 arrays were applied to SELDI-TOF-MS to detect proteomic differences before and after infection.The chip detected a series of differentially expressed protein peaks.Interestingly,both peaks at 16 912 Da and 17 581 Da corresponded precisely with the molecular mass of ISG 15,which may participate in antiviral activity during the process of infection.Thus,the results we obtained can serve as a basis to study the pathogenesis of HSV-1 and the interaction between the virus and its host.In addition,they can help in the discovery of new therapeutic targets for treatment of HSV-1 infection.

This study aims to explore the improvement and the mechanism of the Alisma plantago-aquatica Linn. (ApL) on chronic glomerulonephritis (CGN). All animal experiments were followed the regulation of the Experimental Animal Ethical Committee of Shanghai University of Traditional Chinese Medicine. CGN mouse model was established by a single tail-vein injection of doxorubicin (Dox) (20 mg·kg^-1). One week after Dox administration, the mice received water extract of ApL (85 and 255 mg·kg^-1) by gavage once a day for 14 days. At the end of experiment, the urine albumin-to-creatinine ratio (ACR), serum albumin (ALB), blood urea nitrogen (BUN) and serum creatinine (SCr) were detected, kidney histopathological H&E staining was analyzed. Active ingredients and action targets of ApL were collected from TCMSP database, and CGN-related targets were obtained from Genecards database. STRING platform was employed to perform protein-protein interaction (PPI), and Metascape platform was used for KEGG pathway and GO enrichment analysis. The results of experiments demonstrated that ApL (85 and 255 mg·kg^-1) could reduce the ACR and the content of SCr and BUN, and increase the content of ALB in mice. Network pharmacology results predicted that nuclear factor kappa-B (NF-κB)-related pathway and biological process of oxidoreductase activity regulation may be involved in the ApL-provided amelioration on CGN. The verification results showed that ApL could inhibit the activation of NF-κB and the expression of inflammatory factors in mice, and reduce the activity of renal myeloperoxidase (MPO). Meanwhile, ApL promoted the activation of nuclear factor erythroid 2-related factor 2 (Nrf2) and increased the expression of its downstream gene mRNA, and reduced the level of renal malondialdehyde (MDA) and reactive oxygen species (ROS), and further elevated renal glutathione (GSH) level. Based on network pharmacology combined experiments, this study found that ApL may improve CGN in mice through multiple targets and multiple pathways, in which the inhibition of NF-κB signaling and the activation of Nrf2 signaling may be important mechanisms involved.

OBJECTIVETo investigate the relationship between smoking and gingival crevicular fluid volume (GCF), level of elastase (EA) in 37 severe periodontitis patients before and after 1 month periodontal initial treatment.

METHODSThe GCF samples were collected from 122 sites in 22 heavy smokers (>or= 20 cigarettes/day) and 90 sites in 15 non-smokers before and after 1 month periodontal initial treatment. There is no difference (P > 0.05) on pocket depth between smoking sites (5.6 +/- 1.2) mm and non-smoking sites (5.4 +/- 1.2) mm at baseline. The volume of each GCF sample was measured by Periotron 6000 and the elastase in GCF were determined by substrate (meosuc-als-als-pro-val-NA) method.

RESULTSAfter non-surgical treatment both GCF volume and elastase level were significantly decreased (P < 0.001) in both smokers and non-smokers. But the decrease of GCF volume (91 sites, 74.6%) and elastase level (70 sites, 76.1%) in smokers were significant lower (P < 0.01) than non-smokers (GCF, 88 sites, 97.8%; EA, 56 sites, 93.3%).

CONCLUSIONThese findings suggest that smoking has effect on gingival crevicular fluid volume and elastase level of patients with periodontitis.

Adult ; Gingival Crevicular Fluid ; enzymology ; physiology ; Humans ; Male ; Middle Aged ; Pancreatic Elastase ; analysis ; Periodontitis ; metabolism ; therapy ; Smoking ; adverse effects

OBJECTIVETo determine the incidence of work-related acute pesticide poisoning among farmers in Jiangsu Province and to identify the risk factors.

METHODSMulti-stage stratified sampling and cluster sampling methods were used to randomly select 1490 farmers from the south (Z village), centre (Y village) and north (X village) of Jiangsu Province. The database was established by EpiData 3.1. SPSS 17.0 and SAS 9.13 were used to do chi-square test, trend chi-square test, single factor and multi-factor logistic regression analysis.

RESULTSAmong 1490 farmers, 121 people had work-related acute pesticide poisoning in the past year. The incidence rate in Jiangsu Province was 8.1%. 118 farmers (97.5%) were mild pesticide poisoning.3 farmers (2.5%) were moderate pesticide poisoning. The incidence rate of Work-related acute pesticide poisoning in X village of North Jiangsu Province (13.5%, 68/505) is obviously higher than that in Y village of Central Jiangsu Province (4.8%, 19/399) and Z village of South Jiangsu Province (5.8%, 34/586), and the difference is statistically significant (χ(2) = 29.60, P < 0.01). The incidence rate of Work-related acute pesticide poisoning in female (10.4%, 58/557) is obviously higher than that in male (6.8%, 63/933), and the difference is statistically significant (χ(2) = 6.26, P < 0.05). There are lack of safety guidance, unsafety application methods (without alternate row spraying, without backward application, without down-wind application) and risk behaviors in the spraying (without read labels, preparing pesticides without gloves, wiping sweat with hand (s), equipment leakage, body pollution by pesticide, working when feeling sick, without bath after work). If farmers have risk behaviors above, the incidence rate of Work-related acute pesticide poisoning will be obviously higher than farmers who receive safety guidance and have no such dangerous behaviors, and all the differences are statistically significant (P < 0.05). The group of 24 ∼ 34 years old has the highest risk of work-related acute pesticide poisoning (OR = 7.15).

CONCLUSIONThe incidence rate of work-related acute pesticide poisoning among farmers in Jiangsu Province was higher. Effective measures are needed to prevent and reduce the occurrence of work-related acute pesticide poisoning. We can teach the farmers who spray pesticides about safety guidance, safety application methods and avoiding risky behaviors.

Adult ; Aged ; Agricultural Workers' Diseases ; epidemiology ; Agriculture ; methods ; Female ; Humans ; Incidence ; Male ; Middle Aged ; Occupational Exposure ; Pesticides ; poisoning ; Risk Factors

OBJECTIVETo observe the changes of clinical conditions, and measure the GCF volume during the experimental gingivitis in Chinese for studying the relationship between the GCF volume and the development of gingival inflammation.

METHODS11 young male subjects with healthy gingiva, who had no systemic diseases, were selected for this study. GCF samples (18 teeth/person) were collected with strips of filter paper and the clinical parameters were recorded at the baseline (0 day), the 7th, 14th, 21st day (without oral hygiene), and 28th day (7 days after reestablishing oral hygiene) during experimental gingivitis. The GCF volume was measured by weighting.

RESULTSDuring the experimental gingivitis, all of the clinical parameters plaque index (PLI), bleeding index (BI), gingival index (GI) and probing index (PD) increased gradually following the plaque assembling, there were significant differences comparing the data of baseline with the data of afterwards without oral hygiene. On the 28th day, the data reduced to the level of baseline rapidly. The amount of GCF had the same tendency with the clinical parameters during the experimental gingivitis. There was positive correlation between the amount of GCF and clinical parameters.

CONCLUSIONThe amount of GCF can reflect the development of gingival inflammation.

Adult ; Dental Plaque ; complications ; Diet ; Gingival Crevicular Fluid ; physiology ; Gingivitis ; etiology ; Humans ; Male

OBJECTIVETo investigate the safety, medium-term and long-term efficacy of transradial percutaneous coronary intervention for unprotected left main coronary artery lesions with 6 French guiding catheter.

METHODSSixty-one patients with unprotected left main coronary artery lesions were treated by 6 French transradial percutaneous coronary intervention between January 2008 and December 2009. The mean age of patients was (66.03 ±10.02)years (44-87). Among 61 cases, 40 had hypertension and 14 had diabetes mellitus; 22 had a history of smoking. The average left ventricle ejection fraction was (62.96 ±12.15)% (range: 28-86) and the average plasma creatinine level was (82.92 ±18.30)μmol/L (range: 44-130). The major adverse cardiac events (MACE) after the procedure were evaluated.

RESULTSProcedural success was achieved in all cases. A total of 67 stents were implanted. No in-hospital death occurred. Mean clinical follow-up period was (26.25 ±5.92) months (range: 19-44 months). MACE developed in 6 cases (9.8%) during the follow-up period, including 2 death (3.3%) and 4 case of target lesion revascularization (6.6%). Compared with low-risk group (SYNTAX score<33), MACE was increased in the high-risk group (SYNTAX score>32).

CONCLUSION6 French transradial percutaneous coronary intervention for patients with unprotected left main coronary artery lesions is safe and feasible procedure with desirable medium-and long-term outcomes.

Adult ; Aged ; Aged, 80 and over ; Coronary Artery Disease ; therapy ; Female ; Follow-Up Studies ; Humans ; Male ; Middle Aged ; Percutaneous Coronary Intervention ; methods ; Radial Artery ; Treatment Outcome

OBJECTIVETo clarify drug properties of components in Euodiae Fructus.

METHODThe rat cold syndrome model was induced by cold water stress method. The content of neurotransmitters sand hormones such as DA, 5-HT, NE, AChE and 17-OHCS in serum of model rats were taken as the indexes to evaluate drug properties of components in Euodiae Fructus.

RESULTEuodiae Fructus and its components could correct or relief the content of energy metabolism and substance metabolism-related neurotransmitters sand hormones in serum of model rats with water-stressed cold syndrome.

CONCLUSIONEuodiae Fructus and its components are proved to show hot property.

Animals ; Cold Temperature ; adverse effects ; Disease Models, Animal ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Female ; Gastric Mucosa ; drug effects ; pathology ; Male ; Medicine, Chinese Traditional ; Neurotransmitter Agents ; blood ; Rats ; Rats, Wistar ; Stomach Ulcer ; blood ; drug therapy ; physiopathology ; Stress, Physiological

OBJECTIVETo investigate the relationship between obesity and aggressive periodontitis (AgP).

METHODSA total of 151 AgP patients and 94 healthy controls were recruited. Body weight and height were measured, and body mass index (BMI) was calculated. Periodontal clinical parameters were examined. Logistic regression analysis was used to evaluate the association between obesity and AgP.

RESULTSThe mean value of probing depth and clinical attachment loss were (4.8 +/- 2.1) mm and (4.7 +/- 2.8) mm respectively in AgP patients. The numbers of obese and underweight patients in AgP group were 20 and 41 respectively. Five and eight subjects in control group were with BMI values lower and higher than normal range respectively. Both under weight and obesity were risk indicators for AgP, and the OR were 3.60 (P < 0.05) and 4.61 (P < 0.01) respectively.

CONCLUSIONSUnderweight and obesity may be risk indicators for AgP.

Adult ; Aggressive Periodontitis ; etiology ; Case-Control Studies ; Female ; Humans ; Male ; Middle Aged ; Obesity ; epidemiology ; Periodontal Attachment Loss ; Risk Factors

OBJECTIVETo determine the serum levels of lipid and glucose in patients with periodontitis.

METHODSThe patients with aggressive periodontitis (AgP, n = 117) and chronic periodontitis (CP, n = 40) and healthy controls (n = 37) were recruited. Peripheral blood sample was obtained from each subject by venipuncture. Serum chemistry variables including total cholesterol, triglycerides, and fasting glucose were analyzed by biochemical analyzer.

RESULTSThe average serum levels of triglycerides and glucose of the patients with AgP [(1.09 +/- 0.79) mmol/L and (5.07 +/- 0.66) mmol/L] were both significantly higher than those of the control group [(0.94 +/- 0.28) mmol/L and (4.62 +/- 0.64) mmol/L], P < 0.05. Higher serum levels of total cholesterol and glucose were also observed in patients with CP [(4.61 +/- 1.23) mmol/L and (5.40 +/- 1.01) mmol/L] compared with controls [(4.49 +/- 0.78) mmol/L and (4.62 +/- 0.64) mmol/L], P < 0.05. Serum level of total cholesterol in AgP group was positively associated with percentage of severe sites (r = 0.25, P < 0.01).

CONCLUSIONSPatients with periodontitis may have altered serum lipid and glucose levels compared with controls.

Adolescent ; Adult ; Blood Glucose ; metabolism ; Cholesterol ; blood ; Female ; Humans ; Male ; Middle Aged ; Periodontitis ; blood ; Triglycerides ; blood ; Young Adult

OBJECTIVETo evaluate the prevalence of putative periodontal microorganism in Chinese patients with aggressive periodontitis (AgP).

METHODSA total of 72 subgingival plaque samples were collected from 55 AgP patients and 17 healthy subjects. Seven putative periodontal microorganisms including Actinobacillus actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), Tannerella forsythensis (Tf), Treponema denticola (Td), Campylobacter rectus (Cr), Prevotella intermedia (Pi) and Prevotella nigrescens (Pn) were detected by using 16S rRNA based polymerase chain reaction (PCR).

RESULTSThe prevalence of Aa in AgP patients was very low (1.8%), while Pg, Tf, Td and Cr were more frequently detected in AgP patients (prevalence: 81.8%, 83.6%, 80% and 81.8%) than in healthy controls (prevalence: 17.6%, 11.8%, 5.9%, 29.4%, P < 0.01).

CONCLUSIONSThe prevalence of Pg, Tf, Td and Cr were very high in aggressive periodontitis patients, and the combination of these bacteria may play an important role in AgP.

Adolescent ; Adult ; Aggregatibacter actinomycetemcomitans ; isolation & purification ; Aggressive Periodontitis ; microbiology ; Bacterial Typing Techniques ; Campylobacter rectus ; isolation & purification ; Case-Control Studies ; Dental Plaque ; microbiology ; Dental Plaque Index ; Female ; Humans ; Male ; Polymerase Chain Reaction ; Treponema denticola ; isolation & purification ; Young Adult