Human herpes simplex virus 1 (HSV-1) causes facial,ocular,and encephalitic disease and is associated with latent infection and cancer.Here,we developed a means of studying the pathogenesis of HSV-1 infection at the protein level by using the SELDI Protein Chip to detect changes of protein expression in Vero cells cultured in vitro.After infection with HSV-1 and culture for 12,24 or 48 h,cells were harvested and lysed.IMAC3 arrays were applied to SELDI-TOF-MS to detect proteomic differences before and after infection.The chip detected a series of differentially expressed protein peaks.Interestingly,both peaks at 16 912 Da and 17 581 Da corresponded precisely with the molecular mass of ISG 15,which may participate in antiviral activity during the process of infection.Thus,the results we obtained can serve as a basis to study the pathogenesis of HSV-1 and the interaction between the virus and its host.In addition,they can help in the discovery of new therapeutic targets for treatment of HSV-1 infection.

This study aims to explore the improvement and the mechanism of the Alisma plantago-aquatica Linn. (ApL) on chronic glomerulonephritis (CGN). All animal experiments were followed the regulation of the Experimental Animal Ethical Committee of Shanghai University of Traditional Chinese Medicine. CGN mouse model was established by a single tail-vein injection of doxorubicin (Dox) (20 mg·kg^-1). One week after Dox administration, the mice received water extract of ApL (85 and 255 mg·kg^-1) by gavage once a day for 14 days. At the end of experiment, the urine albumin-to-creatinine ratio (ACR), serum albumin (ALB), blood urea nitrogen (BUN) and serum creatinine (SCr) were detected, kidney histopathological H&E staining was analyzed. Active ingredients and action targets of ApL were collected from TCMSP database, and CGN-related targets were obtained from Genecards database. STRING platform was employed to perform protein-protein interaction (PPI), and Metascape platform was used for KEGG pathway and GO enrichment analysis. The results of experiments demonstrated that ApL (85 and 255 mg·kg^-1) could reduce the ACR and the content of SCr and BUN, and increase the content of ALB in mice. Network pharmacology results predicted that nuclear factor kappa-B (NF-κB)-related pathway and biological process of oxidoreductase activity regulation may be involved in the ApL-provided amelioration on CGN. The verification results showed that ApL could inhibit the activation of NF-κB and the expression of inflammatory factors in mice, and reduce the activity of renal myeloperoxidase (MPO). Meanwhile, ApL promoted the activation of nuclear factor erythroid 2-related factor 2 (Nrf2) and increased the expression of its downstream gene mRNA, and reduced the level of renal malondialdehyde (MDA) and reactive oxygen species (ROS), and further elevated renal glutathione (GSH) level. Based on network pharmacology combined experiments, this study found that ApL may improve CGN in mice through multiple targets and multiple pathways, in which the inhibition of NF-κB signaling and the activation of Nrf2 signaling may be important mechanisms involved.

OBJECTIVETo investigate the safety, medium-term and long-term efficacy of transradial percutaneous coronary intervention for unprotected left main coronary artery lesions with 6 French guiding catheter.

METHODSSixty-one patients with unprotected left main coronary artery lesions were treated by 6 French transradial percutaneous coronary intervention between January 2008 and December 2009. The mean age of patients was (66.03 ±10.02)years (44-87). Among 61 cases, 40 had hypertension and 14 had diabetes mellitus; 22 had a history of smoking. The average left ventricle ejection fraction was (62.96 ±12.15)% (range: 28-86) and the average plasma creatinine level was (82.92 ±18.30)μmol/L (range: 44-130). The major adverse cardiac events (MACE) after the procedure were evaluated.

RESULTSProcedural success was achieved in all cases. A total of 67 stents were implanted. No in-hospital death occurred. Mean clinical follow-up period was (26.25 ±5.92) months (range: 19-44 months). MACE developed in 6 cases (9.8%) during the follow-up period, including 2 death (3.3%) and 4 case of target lesion revascularization (6.6%). Compared with low-risk group (SYNTAX score<33), MACE was increased in the high-risk group (SYNTAX score>32).

CONCLUSION6 French transradial percutaneous coronary intervention for patients with unprotected left main coronary artery lesions is safe and feasible procedure with desirable medium-and long-term outcomes.

Adult ; Aged ; Aged, 80 and over ; Coronary Artery Disease ; therapy ; Female ; Follow-Up Studies ; Humans ; Male ; Middle Aged ; Percutaneous Coronary Intervention ; methods ; Radial Artery ; Treatment Outcome

OBJECTIVETo determine the incidence of work-related acute pesticide poisoning among farmers in Jiangsu Province and to identify the risk factors.

METHODSMulti-stage stratified sampling and cluster sampling methods were used to randomly select 1490 farmers from the south (Z village), centre (Y village) and north (X village) of Jiangsu Province. The database was established by EpiData 3.1. SPSS 17.0 and SAS 9.13 were used to do chi-square test, trend chi-square test, single factor and multi-factor logistic regression analysis.

RESULTSAmong 1490 farmers, 121 people had work-related acute pesticide poisoning in the past year. The incidence rate in Jiangsu Province was 8.1%. 118 farmers (97.5%) were mild pesticide poisoning.3 farmers (2.5%) were moderate pesticide poisoning. The incidence rate of Work-related acute pesticide poisoning in X village of North Jiangsu Province (13.5%, 68/505) is obviously higher than that in Y village of Central Jiangsu Province (4.8%, 19/399) and Z village of South Jiangsu Province (5.8%, 34/586), and the difference is statistically significant (χ(2) = 29.60, P < 0.01). The incidence rate of Work-related acute pesticide poisoning in female (10.4%, 58/557) is obviously higher than that in male (6.8%, 63/933), and the difference is statistically significant (χ(2) = 6.26, P < 0.05). There are lack of safety guidance, unsafety application methods (without alternate row spraying, without backward application, without down-wind application) and risk behaviors in the spraying (without read labels, preparing pesticides without gloves, wiping sweat with hand (s), equipment leakage, body pollution by pesticide, working when feeling sick, without bath after work). If farmers have risk behaviors above, the incidence rate of Work-related acute pesticide poisoning will be obviously higher than farmers who receive safety guidance and have no such dangerous behaviors, and all the differences are statistically significant (P < 0.05). The group of 24 ∼ 34 years old has the highest risk of work-related acute pesticide poisoning (OR = 7.15).

CONCLUSIONThe incidence rate of work-related acute pesticide poisoning among farmers in Jiangsu Province was higher. Effective measures are needed to prevent and reduce the occurrence of work-related acute pesticide poisoning. We can teach the farmers who spray pesticides about safety guidance, safety application methods and avoiding risky behaviors.

Adult ; Aged ; Agricultural Workers' Diseases ; epidemiology ; Agriculture ; methods ; Female ; Humans ; Incidence ; Male ; Middle Aged ; Occupational Exposure ; Pesticides ; poisoning ; Risk Factors

OBJECTIVETo investigate the relationship between smoking and gingival crevicular fluid volume (GCF), level of elastase (EA) in 37 severe periodontitis patients before and after 1 month periodontal initial treatment.

METHODSThe GCF samples were collected from 122 sites in 22 heavy smokers (>or= 20 cigarettes/day) and 90 sites in 15 non-smokers before and after 1 month periodontal initial treatment. There is no difference (P > 0.05) on pocket depth between smoking sites (5.6 +/- 1.2) mm and non-smoking sites (5.4 +/- 1.2) mm at baseline. The volume of each GCF sample was measured by Periotron 6000 and the elastase in GCF were determined by substrate (meosuc-als-als-pro-val-NA) method.

RESULTSAfter non-surgical treatment both GCF volume and elastase level were significantly decreased (P < 0.001) in both smokers and non-smokers. But the decrease of GCF volume (91 sites, 74.6%) and elastase level (70 sites, 76.1%) in smokers were significant lower (P < 0.01) than non-smokers (GCF, 88 sites, 97.8%; EA, 56 sites, 93.3%).

CONCLUSIONThese findings suggest that smoking has effect on gingival crevicular fluid volume and elastase level of patients with periodontitis.

Adult ; Gingival Crevicular Fluid ; enzymology ; physiology ; Humans ; Male ; Middle Aged ; Pancreatic Elastase ; analysis ; Periodontitis ; metabolism ; therapy ; Smoking ; adverse effects

OBJECTIVETo observe the changes of clinical conditions, and measure the GCF volume during the experimental gingivitis in Chinese for studying the relationship between the GCF volume and the development of gingival inflammation.

METHODS11 young male subjects with healthy gingiva, who had no systemic diseases, were selected for this study. GCF samples (18 teeth/person) were collected with strips of filter paper and the clinical parameters were recorded at the baseline (0 day), the 7th, 14th, 21st day (without oral hygiene), and 28th day (7 days after reestablishing oral hygiene) during experimental gingivitis. The GCF volume was measured by weighting.

RESULTSDuring the experimental gingivitis, all of the clinical parameters plaque index (PLI), bleeding index (BI), gingival index (GI) and probing index (PD) increased gradually following the plaque assembling, there were significant differences comparing the data of baseline with the data of afterwards without oral hygiene. On the 28th day, the data reduced to the level of baseline rapidly. The amount of GCF had the same tendency with the clinical parameters during the experimental gingivitis. There was positive correlation between the amount of GCF and clinical parameters.

CONCLUSIONThe amount of GCF can reflect the development of gingival inflammation.

Adult ; Dental Plaque ; complications ; Diet ; Gingival Crevicular Fluid ; physiology ; Gingivitis ; etiology ; Humans ; Male

OBJECTIVETo clarify drug properties of components in Euodiae Fructus.

METHODThe rat cold syndrome model was induced by cold water stress method. The content of neurotransmitters sand hormones such as DA, 5-HT, NE, AChE and 17-OHCS in serum of model rats were taken as the indexes to evaluate drug properties of components in Euodiae Fructus.

RESULTEuodiae Fructus and its components could correct or relief the content of energy metabolism and substance metabolism-related neurotransmitters sand hormones in serum of model rats with water-stressed cold syndrome.

CONCLUSIONEuodiae Fructus and its components are proved to show hot property.

Animals ; Cold Temperature ; adverse effects ; Disease Models, Animal ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Female ; Gastric Mucosa ; drug effects ; pathology ; Male ; Medicine, Chinese Traditional ; Neurotransmitter Agents ; blood ; Rats ; Rats, Wistar ; Stomach Ulcer ; blood ; drug therapy ; physiopathology ; Stress, Physiological

China ; Humans ; Maximum Allowable Concentration ; Occupational Exposure ; prevention & control

OBJECTIVETo investigate the potential genetic mode of aggressive periodontitis (AgP) in Chinese Han nationality.

METHODSA total of 233 subjects from 73 nuclear families were recruited. All probands were diagnosed according to the criteria of AgP in 1999 classification of periodontal diseases. Ninety parents, 35 siblings and three grandparents and two offspring were examined based on full-mouth periodontal chartings (including parameter of probing depths, attachment loss, bleeding on probing at six sites per tooth) and full-mouth periapical radiographs. The genetic ratio was calculated and analyzed by the methods of Edwards and simple segregation.

RESULTSThe prevalence of AgP in probands' siblings was close to the square root of the prevalence of general population. The segregation ratio was 0.2419, which was close to the theoretical ratio for autosomal recessive inheritance. However, autosomal dominant inheritance could not be rejected in families whose parent(s) suffered from severe chronic periodontitis.

CONCLUSIONSThe genetic heterogeneity of AgP existed in Chinese Han nationality. The genetic mode was autosomal recessive inheritance in general, and autosomal dominant inheritance could not be excluded in families whose parent(s) suffered from severe chronical periodontitis. The results imply the genetic heterogeneity of AgP, and further demonstrate that AgP was a multifactorial disease with major genetic component in the disease etiology.

Aggressive Periodontitis ; epidemiology ; genetics ; Asian Continental Ancestry Group ; genetics ; Chronic Periodontitis ; epidemiology ; genetics ; Female ; Genes, Dominant ; Genes, Recessive ; Genetic Heterogeneity ; Humans ; Male ; Pedigree ; Prevalence ; Surveys and Questionnaires

OBJECTIVETo investigate the relationship between tumor necrosis factor (TNF) A-308 gene polymorphism and aggressive periodontitis (AgP).

METHODSAnti-coagulated peripheral blood samples were obtained from 64 AgP patients, and 78 healthy controls (H). Genomic DNA was extracted from each sample. The TNF A-308 gene polymorphism was detected by standard polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. Data were analyzed by SAS statistical software.

RESULTSThere was no significant difference in distribution of TNF A-308 genotype between AgP patients and healthy controls. But the ORs for genotype GA and allele A were 22.2 and 16.1 respectively in non-smoking male AgP patients.

CONCLUSIONSThe TNF A-308 genotype GA and allele A may be associated with AgP susceptibility in Chinese males.

Aggressive Periodontitis ; genetics ; Alleles ; Female ; Gene Frequency ; Genetic Predisposition to Disease ; Genotype ; Humans ; Male ; Polymorphism, Genetic ; Tumor Necrosis Factor-alpha ; genetics