Hand-Arm Vibration Syndrome ; Humans

Animals ; Cell Culture Techniques ; Cell Differentiation ; Cells, Cultured ; Embryonic Stem Cells ; cytology ; Female ; Hematopoiesis ; Hematopoietic Stem Cells ; cytology ; Male ; Mice

Objective To analyze the clinical and radiographic characterstics of ureteral polyps with hydronephrosis in children.Methods Thirteen patients with ureteral polyps and hydronephrosis were studied retrospectively.All patients underwent abdominal plain film,intravenous pyelogram(IVP)and ultrasound(US)examinations,contrast-enhanced CT scan was performed in 10 cases.Results Intermittent or recurrent abdominal pain with painless hematuria was presented in most cases.Hydronephrosis was demonstrated in radiographic images.IVP delineated the dilatation of the ureter and filling defects within the ureteral lumen in 5 cases.Computed tomography(CT)showed all abnormal changes of ureter and irregular intraluminal soft tissue masses in 6 cases.Moderate and low echoic structures were showed in ureters by US in 2 cases.Conclusion US and CT,as an important imaging modalities,can improve the diagnostic accuracy for ureteral polyps.

AIM: To investigate the status of myopia in students from different kinds of schools in Shantou city, China.
METHODS: In this cross-sectional study, 2 418 students from 4 middle and high schools in Shantou were surveyed through randomized cluster sampling. Ophthalmic examination and auto-refraction were performed for all subjects.
RESULTS: Among all 2 394 students examined. The myopia rates for students from middle and high school were 48. 07% and 68. 28%. In normal middle school, students from rural area had lower rate of myopia than students from the urban area (χ2 = 13. 879, P<0. 01 ). Students from key rural high schools had lower rate of myopia than students from key urban high school (χ2 =175. 231, P<0. 01).
CONCLUSION:The prevalence of myopia in the middle school of urban area in Shantou was higher than rural area. The increasing rate of myopia in youngsters is correlated with study stress. In order to decrease the myopia rate, it is extremely important to improve study environment in students.

The genes of maltooligosyl trehalose synthase (MTSase) and maltooligosyl trehalose tetrahydrolase(MTHase) from Sulfolobus solfataricus ATCC 35092 were amplified using PCR. The expression plasmids, pTrc99a-MTSase and pTrc99a-MTHase, were constructed by inserting these two DNA fragments into E. coli expression vector pTrc99a. The specific activity of MTSase and MTHase in E. coli BL21(DE3) at optimal fermentation conditions reached 31.3U/g (wet cell) and 403U/g (wet cell), respectively. The biotransformation of partially hydrolyzed starch to trehalose catalyzed by MTSase and MTHase was carried out at 75℃ and pH 5.0. The highest yield of trehalose (ca. 53.6%) was gained when the original starch concentration was 15%(w/v) and the DE value was 10.

0.05).Conclusion Febrile seizures seldomly cause severe neurological damage.

Background Metadherin is a newly discovered oncogene.It is highly expressed in some solid tumors and plays a significant role in invasion and metastasis of neoplasm as an important biological marker of aggressive cancers.However,there is little data available for the relationship between metadherin expression and clinicopathologic features of retinoblastoma(RB).Objective This study was to investigate the expression of the metadherin in RB cell lines and specimens as well as its clinical significance.Methods The expression of metadherin mRNA in different metastatic potential of RB cell lines(Y79,WERI-RB1 and SO-RB50)was detected by real-time PCR,and the protein expression of metadherin (MDTH)in paraffin-embedded RB tissue was detected by immunohistochemistry.The relevance of metadherin expression to clinical histopathological features was statistically analyzed.Results The relative expression value of metadherin mRNA was 6.11±0.17,6.21±0.21 and 3.97±0.17 in Y79,SORB50,WERI-RB1,respectively,with a significant difference among the three types of cell lines(F =142.643,P＜0.05).The relative expression value of metadherin mRNA was significantly higher in Y79 or SO-RB50 than that in W ERI-RB1 (P=0.000).The expression of MTDH protein mostly located in the cellular membrane and cytoplasm.Among the 54 RB sections,35 (64.81％)showed a high intensity in expression of metadherin protein.The total score of metadherin protein expression was higher in the E stage of RB than that of D stage(P=0.035),in the patients with high-risk factor than those without high-risk factor(P=0.002)and the cases with optic nerve invasion compared to non-invasion (P＝0.017).However,no significant differences were found in the expression of metadherin protein between different ages (P =0.579),different genders (P =0.513),bilateral eyes (P =0.305),different disease courses (P=0.860),various types of differentiation (P =0.537),different degree of the ehoroid invasion (P =0.238),and with or without invasion of the anterior ocular segment (P =0.579).Conclusions Metadherin appears to be highly expressed in RB cells.The activation of the MTDH gene is associated with invasion and metastasis of RB.These results imply that the dynamic change of metadherin expression probably is a prognostic indicator of RB.

OBJECTIVETo observe the regulation of heart rate to cardiac pump function in the phase of negative force-frequency relationship and their possible mechanisms.

METHODSThe left ventricular pressure, aortic pressure, and cardiac output were measured in isolated working heart of rat from 240 to 300 beats/min of pacing rate.

RESULTSCardiac output of isolated working heart was decreased by a proximally 20% (P < 0.01) with the increase in the pacing rate from 240 to 300 beats/min. Left ventricular end-systolic pressure (LVESP) was declined by 4.8% (P < 0.05), but left ventricular end-diastolic pressure (LVEDP) was elevated by 139% (P < 0.01) with an increase in the pacing rate. Left atrium was enlarged at 300 beats/min of pacing rate. The time from peak to 75% relaxation in left ventricular pressure was shortened with the increased pacing rate. Pressure at aortic valve close was raised (P < 0.01) and ejection duration was shortened with the increased pacing rate (P < 0.01).

CONCLUSIONThose above results suggest that there are different mechanisms between the depressed cardiac output at higher heart rate and negative force-frequency relationship. The frequency-dependent acceleration of relaxation facilitates the decline of left ventricular pressure, and then may elevate the pressure of aortic valve close in the condition that the shape of aortic pressure curve stays the same. Therefore, the ejection duration is shortened at higher pacing rate. The shortened ejection duration may induce a decrease in stroke volume of the left ventricle. The increment of heart rate is not enough to compensate the decreased stroke volume. Finally, cardiac output shows a decrease at higher heart rate.

Animals ; Blood Pressure ; Cardiac Output ; Heart Rate ; physiology ; Male ; Pressoreceptors ; Rats ; Rats, Sprague-Dawley

Animals ; Antineoplastic Agents, Phytogenic ; isolation & purification ; pharmacology ; Cholesterol ; biosynthesis ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; HIV Protease ; metabolism ; HIV Protease Inhibitors ; pharmacology ; Humans ; Plants, Medicinal ; chemistry ; Platelet Aggregation ; drug effects ; Polysaccharides ; isolation & purification ; pharmacology ; Reishi ; chemistry ; Sterols ; isolation & purification ; pharmacology ; Triterpenes ; isolation & purification ; pharmacology

OBJECTIVETo analyze micromovement of distal tihiofihular joints in different motion range of ankle joint ,and define the micromovement characteristic and range of distal tibiofihular joints.

METHODSTwelve normal Chinese were chosen. There were 9 males and 3 females, aged from 19 to 37 years old with an average (26.5 +/- 0.5) years. Detection terminals of laser photographic scanner were installed near the highest point between medial malleolus and lateral malleolos, the change of detection terminals on the position of dorsiflex, extension, introversion and eversion of ankle joint were scanned by 3D-laser scanner. The displacement of two detection terminals on the X ,Y and Z-axis (X-axis stands for the vertical-axis between coronal plane and Z-axis Y-axis stands for the vertical-axis between sagittal plane and Z-axis Z-axis stands for macroaxis of tibia).

RESULTSAlong with increased range of motion on the position of dorsiflex ,extension, introversion and eversion of ankle joint, the range of micromovement of distal tibiofibolar joints increased too. The max-displacement of X, Y and Z were respectively (1.04 +/- 0.12) mm, (1.70 +/- 0.16) mm and--(0.87 +/- 0.10) mm.

CONCLUSION3D-laser scanner can be used to determine the detailed displacement of distal tibiofibolar joint on the X , Y and Z, and measure the motion of distal tibiofibular joint. The method can be used to study the pathologic change of distal tibiofibular joint ,and provide basic biomechnics data for internal fixtor fitting for the characteristic of distal tibiofibular joint.

Adult ; Ankle Joint ; physiology ; Asian Continental Ancestry Group ; Female ; Fibula ; Humans ; Male ; Movement ; Range of Motion, Articular ; Tibia ; Young Adult