1.Expression of Serum miR-141 and miR-224 in Patients with Non-Small Cell Lung Cancer and Its Clinical Significance
Wen-Ke LIN ; Ji-Fang WU ; Zhi-Ang ZHENG
Journal of Modern Laboratory Medicine 2018;33(2):46-50
Objective To investigate the expression of miR-141 and miR-224 in non-small cell lung cancer (NSCLC) and its clinical significance.Methods The levels of serum miR-141,miR-224 and squamous cell carcinoma associated antigen (SCC-Ag) were detected by RT-PCR in 128 patients with NSCLC,60 patients with benign lesions (benign group) and 60 healthy subjects (control group).To analyze the relationship between the expression levels of miR-141 and miR-224 and the clinicopathological features of NSCLC.The sensitivity and specificity of miR-141,miR-224 and SCC-Ag in the diagnosis of NSCLC were evaluated by ROC curve,and the correlation between serum miR-141 and miR-224,SCC-Ag were analyzed by Pearson correlation analysis in NSCLC patients.Results The levels of serum miR-141,miR-224 and SCC-Ag in NSCLC group were significantly higher than those in the benign group and the control group[miR-141(2-△△Ct):2.56±0.48 vs 1.08±0.24 and 1.02±0.21,miR-224 (2-△△Ct):3.94±0.82 vs 1.42±0.35 and 1.26±0.30,SCC-Ag (ng/ml):2.75±1.36 vs 0.64±0.47 and 0.52±0.24,all P<0.01].In patients with NSCLC,the levels of serum miR-141 and miR-224 were correlated with pathological stage,pathological grade and lymph node metastasis (P<0.05).ROC curve analysis showed that the optimal cut-off values of serum miR-141,miR 224 and SCC Ag (ng/ml) for diagnosis of NSCLC were 1.84,2.85 and 2.03,respectively.The AUC (0.913) of the three combined diagnosis of NSCLC was the largest,and the sensitivity and specificity were better,with 92.5 % and 82.7 %,respectively.Pearson correlation analysis showed that serum miR-141 were positively corre lated with miR-224 and SCC-Ag (r=0.782,0.594,all P<0.01),and serum miR 224 was positively correlated with SCC-Ag (r=0.594,P<0.01).Conclusion Serum miR-141 and miR-224 are up-regulated in patients with NSCLC and are associated with clinicopathological features,and may be a new biomarker for the diagnosis of NSCLC.
2.Defibrillation energy measurement and result uncertainty assessment in quality control of defibrillator
zhi Da YI ; Ang YAN ; zheng Chu HU ; ping Ya HOU ; Quan ZHOU
Chinese Medical Equipment Journal 2017;38(7):93-96
Objective To explore the application of defibrillation energy measurement and result uncertainty assessment to defibrillator quality control.Methods The released energy of the defibrillator was measured in standardized technology and environmental conditions,and uncertainty assessment was carried out according to the criteria JJF 1059-2012 Assessment and expression of measurement uncertainty,and then the quality of the defibrillator was executed based on uncertainty.Results Uncertainty assessment contributed to the evaluation of the defibrillator quality,and provided guidance to medical engineering departments in the defibrillator quality control.Conclusion Uncertainty assessment of the defibrillation energy measurement facilitates the quality control,and thus can be promoted in the quality control of other likely medical devices such as respirator,infusion pump and electrosphygmomanometer.