Objective:To prepare renal cell carcinoma-dendritic cell fusion vaccines,and observe the biological characteristics of the fusion cells,to provide theoretical basis and experimental data for further research of DC based cancer vaccines. Methods:The renal cell carcinoma and dendritic cells were fused by using polyethylene glycol(PEG). The growth curve of fusion cells,morphological characteristics,expression of costimulation molecules,abilities to amplify T lymphocytes and grow into new tumors were detected. Results:Renal cell carcinoma and dendritic cells could be fused effectively by PEG.The fusion cells had weak proliferation abilities and were unable to grow into new tumors in immunol-deficiency animals,but the costimulation molecules expression such as CD86 and HLA-DR and the abilities to irritate the proliferation of T lymphocytes were remarkably increased than that of parental control groups(P

Health problems caused by age-related hearing loss can not be ignored, and elderly hearing screening that aimed to detect early and intervene early is of importance. As one of the main screening tools, elderly hearing screening scales are rarely used in China, so the paper mainly reviewed the development and application of hearing screening scales for elderly and provided a reference for future research and clinical application.

Social capital is an invisible capital form that promotes the efficient allocation and utilization of resources.It plays an active role in the prevention and control of chronic diseases.The purpose of this study is to investigate the effect of social capital on the three levels prevention of stroke by means of document retrieval.Social capital is associated with both morbidity and mortality of stroke.Social participation and social support at a high level can promote the rehabilitation of stroke patients,and have a beneficial effect on improving the quality of life and the ability of daily life.Social capital is correlated with stroke related behavioral factors,such as smoking,drinking,obesity,diet and physical activity.People with high social capital stock are more likely to choose healthy behavior patterns,thus reducing the risk of stroke.We should take full account of the social capital factors,and make more comprehensive and effective completion of the three levels of stroke prevention.

Objective: To examine the change of body weight (BW) and blood pressure (BP) in obese rats, clarify relationships between BP and BW and other factors. Methods: Male Spraque-Dawley rats were fed either with normal diet (ND) or high calorie diet (HC) for 20 weeks. BW and BP of tail artery were observed biweekly and tetraweekly respectively; serum leptin and fasting insulin (FINS) were detected by enzyme-linked immunoadsordent assay (ELISA) and radioimmunoassay (RIA) respectively. Fasting plasma glucose (FPG) and free fatty acid(FFA) were measured by conventional means. Results: BW, abdominal fat weight (AFW), ratio of abdominal fat weight to body weight (RF/W), systolic blood pressure (SBP), diastolic blood pressure (DBP), serum levels of leptin and FINS, FPG, FFA increased in the HD group after 20 weeks diet intervention (P<0.05 or P<0.01). SBP was strongly correlated with BW, leptin, FINS and FFA (P<0.05), DBP was correlated with FFA (r=0.47, P<0.05). In addition, leptin was positively correlated with BW, AFW, RF/W, FINS and FFA (P<0.05 or P<0.01). Conclusion: In this study of high calorie-diet induced rats, the gain of BW is accompanied by increased BP. The obese rats have hyperleptinemia, hyperinsulinemia, hyperglycemia and dyslipidemia which may have important effects on the development of obesity-related hypertension. R F/W is the key factor in which affect serum leptin level.

ATM: To investigate the relationship between the oculus dominans and the eye with higher diopter among myopes, and to study the difference between the Worth 4 dot test and hole-in-the-card test.
METHODS: Totally 125 cases were divided into two groups as physiogenic anisometropic group and pathological anisomitropic group according to the degree of binoculus anisometropy. Worth 4 dot test and hole-in-the-card test were used to distinguish oculus dominans respectively based on medical optometry.
RESULTS: In physiogenic anisometropic group, the oculus dominans and the eye with higher diopter among myopes had significant relationship by hole-in-the-card test (Z = -4. 057, P<0. 01) and Worth 4 dot test (Z=-3. 558, P<0. 01). However, in pathological group, No statistical significance was between two tests (P>0. 05); distinguish the oculus dominans had no statistically significant difference between the Worth 4 dot test and hole-in-the-card test (n=125, P>0. 05).
CONCLUSION:The oculus dominans is determined by gene or formed during juvenile period, then myopia anisometropic may affect the choice of that. The Worth 4 dot test has some reference significance for observing oculus dominans.

Gas Chromatography-Mass Spectrometry ; methods ; Humans ; Malathion ; metabolism ; urine

Humans ; Hypertension, Pulmonary ; diagnosis ; therapy

This study aimed to construct full-length cDNA clones of the Japanese encephalitis virus (JEV). SA14-14-2 strain and discuss the feasibility of constructing chimeric viruses for exogenous gene expression based on the JEV genetic skeleton. Long-fragment RT-PCR techniques were applied to amplify JEV cD-NAs, and two amplified fragments with corresponding restriction endonuclease sites at both ends were cloned into the pACYC184 vector sequentially. Using standard molecular techniques, the enhanced green fluorescent protein (EGFP) gene was inserted into the 3' non-coding region of JEV as a reporter gene. After in vitro transcription and transfection procedures, wild-type JEV and chimeric JEV that expressed the EGFP as the reporter gene were successfully rescued. The recovered viruses were characterized by RT-PCR, plaque assays, and direct fluorescence microscopy. After six serial passage generations, the stability of the recovered viruses were studied in terms of virus growth characteristics and structural gene expression. The results showed that cDNA clones of rJEV and rJEV-EGFP were successfully constructed and rescued in BHK-21 cells after in vitro transcription and transfection. Each generation of the recovered viruses was stable and the chimeric virus rJEV-EGFP could stably express EGFP. The findings of this study indicate that both rJEV and rJEV-EGFP could be constructed and rescued in BHK-21 cells, and the JEV SA14-14-2 strain could be obtained as a viral vector to express foreign genes.
Cloning, Molecular ; DNA, Complementary ; genetics ; metabolism ; Encephalitis Virus, Japanese ; genetics ; metabolism ; Encephalitis, Japanese ; virology ; Gene Expression ; Genetic Vectors ; genetics ; metabolism ; Green Fluorescent Proteins ; genetics ; metabolism ; Humans ; Vaccines, Attenuated ; genetics ; metabolism ; Viral Vaccines ; genetics ; metabolism

Aldehydes ; chemistry ; pharmacology ; Chromatography, High Pressure Liquid ; DNA Adducts

Objective To evaluate the efficacy of erlotinib on advanced NSCLC, and observe its adverse events. Methods An open labeled, expanded access program (EAP) was conducted on 19 pathologically confirmed advanced NSCLC patients who had received at least one regimen chemotherapy.Erlotinib (150 mg) was orally administered daily till disease progression or intolerable adverse events developed.The efficacy was evaluated according to RECIST criteria; the adverse events were evaluated according to NCI criteria.Results In 19 patients, the objective response rate was 21.1% (4/19), and the disease control rate was 84.2 % (16/19); the median progression-free survival time was 7.5 months (3-36 months), and the median survival time was 15.9 months (9-39 months).Adverse events were generally mild (grade Ⅰ or Ⅱ ), including skin rash (84.2 %) and diarrhea (57.9 %). One (5.3 %) patient developed grade Ⅲ elevation of serum glutamate pyruvate transaminase.No grade Ⅳ drug-related adverse event occurred.Conclusion Erlotinib is effective and safe for locally advanced or metastatic NSCLC patients who have failed previous chemotherapy.