AIM: To provide the applied ground of the formulation of Rhizoma Phragmitis by setting up a fingerprint of Rhizoma Phragmitis. METHODS:We gained the extracts of Rhizoma phrapmitis by acetone extraction and assayed its extracts by means of HPLC, with a mobile phase of water (1% acetic acid)-water(5% acetonitrile) on inertsil ODS-3 column. an ultraviolet detector was at 250 nm. RESULTS:Fingerprint was established on the basis of the relative retention value and the relative peak area. CONCLUSION: The method is of high sensitivity and good reproducibility.