Female ; Humans ; Male ; Nasal Septum ; abnormalities ; surgery ; Otorhinolaryngologic Surgical Procedures ; Rhinitis, Allergic, Perennial ; complications ; surgery ; Treatment Outcome

OBJECTIVETo observe the intestinal movement of transient receptor potential vanilloid 1 or vanilloid receptor 1 (TRPV1) knockout mice after stimulated by electroacupuncture (EA), and to primarily explore the roles of TRPV1 receptor in the jejunal motility regulated by acupuncture.

METHODSNormal wild-type CL57BL/6 and TRPV1 gene knockout mice were recruited in two groups, the B6 group and the TRPV1 group, 15 in each group. The thermal threshold and the mechanical pain threshold were respectively detected using JL-F digital photo thermal analyzer instrument and ALMEMO2450 machine. The difference between the two thresholds were compared. Meanwhile, a self-made pressure head was placed in the jejunum. The internal pressure was monitored. When the pressure was stable, 2 mA 2/15 Hz EA at Quchi (LI11), Tianshu (ST25), Shangjuxu (ST37), and Dachangshu (BL25) to observe the changes of intestinal pressure value between before and after EA. The curve of internal pressures was recorded.

RESULTS(1) The stimulation of light/heat and mechanical stimulation were obviously slowed in the TRPV1 group than in the B6 group (P < 0.01). (2) In the intestinal pressure observation experiment, the bowel movement was not obviously seen in the two groups when acupunctured at Quchi (LI11) and Shangjuxu (ST37) (P > 0.05). (3) Acupuncture at Tianshu (ST25) and Dachangshu (BL25) of TRPV1 knockout mice could lead to intestinal movement (P < 0.05), mainly inhibited bowel movement. The changing degree was equivalent to that of B6 mice.

CONCLUSIONSUnder the physiological condition, TRPV1 might be mediated by thermal and mechanical stimulation. But TRPV1 mediated acupuncture effect was quite complex, indicating TRPV1 mice might be one of intestinal movement mediating factors.

Animals ; Electroacupuncture ; Gastrointestinal Motility ; Jejunum ; physiology ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; TRPV Cation Channels ; physiology

Chemokines are major regulators of cell transformation and adhesion.Recent study has demonstrated that CXCR7 can bind to CXCL11 and CXCL12 with high affinity,and the activated CXCR7 may influence tumor invasion and metastasis by regulating extracellular matrix (ECM) and epithelial-mesenchymal transition (EMT) and other signal transduction pathways.Therefore,in-depth study of the molecular mechanisms of CXCR7 in tumor invasion and metastasis may provide a more effective theoretical basis for tumor treatment.

Animals ; Drugs, Chinese Herbal ; pharmacology ; Liver ; pathology ; Liver Cirrhosis ; pathology ; Paeonia ; Rats ; Rats, Sprague-Dawley

Bacterial cellulose (BC) is a natural polymer that has bioactivity, biodegradability and biocompatibility. It displays unique physical, chemical and mechanical properties including high crystallinity, high water holding capacity, nanofibre-network structure, high tensile strength and elastic modulus. Due to its unusual material properties, BC has recently become a kind of attractive biomedical material in the international research.Describes BC's properties, study history and its applications as biomedical materials, especially gives emphasis to introduce the applications of BC on scaffold tissue engineering, artificial blood vessels, artificial skin and the treatment of skin wound, as well as the present study status.

Cholestasis, Intrahepatic ; etiology ; Citrullinemia ; complications ; Humans ; Infant ; Male

Objective To investigate the effects of ulinastatin on the expression of neutrophil CD_(11b) and CD_(18) during orthotopic liver transplantation(OLT).Methods Forty patients with liver diseases,ASAⅢorⅣ, undergoing OLT were randomly divided into two groups.Ulinastatin group(n=20)received intravenous infusion of ulinastatin 3?10~5 IU in 100 ml normal saline after skin incision and repeated every 4 hours thereafter.Control group received same amount of normal saline instead(n=20).Blood samples were taken immediately before skin incision,120 min after skin incision,30 min of anhepatic phase,60 min of neohepatic phase and at the end of operation for measurement of CD_(11b) and CD_(18) expression of neutrophil by flow cytometry.Results CD_(11b)/CD_(18) expression was increased significantly in control group 60 min of neohepatic phase and at the end of operation compared to the level before skin incision,but in ulinastatin group there was no significant change in CD_(11b)/CD_(18) expression during the whole procedures(P＞0.05).CD_(11b)/CD_(18)expression was significantly lower 60 min of neohepatic phase and at the end of operation in ulinastatin group than in control group(P＜0.01).Conclusion Ulinastatin can inhibit the increase in CD_(11b)/CD_(18)expression during OLT,and be helpful for reducing the inflammatory response.

ObjectiveTo discuss the role of transient receptor potential vanilloid 1 (TRPV 1) in the regulation effect of electroacupuncture on gastric motility.MethodTRPV1 gene knock-out mice (KO mice) and wild-type C57BL/6 mice (WT mice) were selected to receive acupuncture at Zusanli (ST36),Quchi (LI11), Zhongwan (CV12), and Weishu (BL21), and the intragastric pressure was observed before and after acupuncture.ResultElectroacupuncture at Zusanli caused both excitation and inhibition in WT mice, predominated by mild excitation, while electroacupuncture at Weishu, Quchi and Zhongwan all caused inhibition effect; in the KO mice, electroacupuncture at Zusanli, Quchi, Zhongwan, and Weishu all inhibited gastric motility.Conclusion TRPV1 bears certain regulating effect on gastric motility, andacupuncture can inhibit the gastric motility in TRPV gene KO mice.

Altitude ; Altitude Sickness ; prevention & control ; Health Services Administration ; Humans