Asthma ; epidemiology ; prevention & control ; therapy ; Child ; China ; epidemiology ; Drug Therapy, Combination ; Humans ; Immunotherapy

Asthma ; complications ; therapy ; Child ; Humans ; Rhinitis, Allergic, Perennial ; complications ; therapy

Objective To investigate the status quo of nursing lack in level three first-class hospitals of Zhongshan city,and analyze the relevant reasons.Methods A sample of 765 nurses in 4 tertiary general hospitals completed the modified Missed Nursing Care Survey.Results Assessment,planning,intervention and evaluation were reported to be missed by 9.86％～39.04％,12.05％～44.38％,3.29％～46.85％ and 12.19％～22.05％ of the survey respondents respectively.Reasons for missed care were lack of labor resources(85.48％～93.84％),lack of material resources(80.00％～80.96％),low efficiency of the team (78.08％～86.58％),poor work habits (70.95％～83.84％),and improvement of patients’ needs (88.90％～93.01％).Conclusions Lack of care is widespread,and the degree of lack of each link is different.Reasons lead to the lack of care of nursing staff are higher patients’ demands to nursing work,shortage of nursing staff and unplanned surge of patients’ number.

Asthma ; epidemiology ; Humans ; Rhinitis, Allergic ; Rhinitis, Allergic, Perennial ; epidemiology

Objective To investigate the knowledge,attitude and behavior of medical staffs on foodborne diseases.Methods To investigate the knowledge,attitude and behavior of medical staffs on foodborne diseases by random sampling following the questionnaire.Results There were 332 medical workers attending the survey.The medical staffs were lack of knowledge for foodborne disease,percent of pass was 34.33％.The pass rate of attitude and behavior were 88.88％ and 98.19％.The knowledge score of medical staff of top three hospital (6.49± 2.18)were lower than the community health service center(7.04±2.26) (P＜0.05),but attitude and behavior score of this two staffs had not statistical differences(P＞0.05).Conclusion It is necessary to help medical staff to improve the attitude of foodborne diseases,and improve the monitoring of underreporting case.

Objective To explore expression of the minichromosome maintenance 2 (MCM2)protein and the mucin-like cell surface adhesion molecule CD24 in non-small cell lung cancer (NSCLC) and their relationship with its prognosis. Methods Seventy-three patients of NSCLC diagnosed for the first time and received surgical treatment in Xuanwu Hospital, Beijing were selected for the study. Expression of the MCM2 and CD24 in pathological specimens of the patients was measured by immunohistochemistry and their relationship with its prognosis was analyzed retrospectively. Results High-level expression of the MCM2 and CD24 was seen in 42 and 54 of 73 NSCLC patients, accounting for 57. 5 percent and 74. 0 percent,respectively. Risk of death for the patients with high-level expression of the MCM2 or the CD4 was significantly higher as compared to those with low-level expression ( P ＜ 0. 05 ). Risk of death for patients with both high-level expression of the MCM2 and CD24 was significantly higher than that in those with only high-level expression of the MCM2 or the CD24 (HR =2. 59, 95%CI 1.40 -4. 80, P=0. 002) and in those with both low-level expression of them ( HR = 15.32, 95 % CI = 2.07 - 113.41, P = 0. 008 ). But there was no significant difference in risk of death between patients with high-level expression of the MCM2 or CD24 and those with low-level expression of both of them ( HR = 5. 60, 95% CI 0. 79 - 44. 82, P = 0. 083 ), and cumulative survival rate of patients with both high-level expression of the MCM2 and CD24 was significantly lower than those with only high-level expression of the MCM2 or the CD24 ( P = 0. 001 ). Conclusions Both expression of the MCM2 and the CD24 are independent prognostic factors for NSCLC and combined detection of the two markers have higher prognostic value for it.

Objective To investigate the expression of MCM2 and its prognostic significance in non-small cell lung cancer (NSCLC). Methods The expression of MCM2 was measured by immunohistochemistry in 73 cases of NSCLC and 10 cases of normal lung tissue. The correlations between the expression of MCM2 and clinic-opathological parameters and prognosis were investigated. Results There was no MCM2 expression in normal lung tissue and positive rate of MCM2 expression was 87.7% in NSCLC. The difference between the two groups was significant (P<0.001). The expression of MCM2 in poorly differentiated NSCLC patients was significantly higher than that in moderately- and well-differentiated NSCLC patients (P=0.008). The expression of MCM2 in patients with squamous carcinoma was higher than that in patients with adenocarcinoma (P=0.005). The hazard ratio was significantly higher(RR=3.389, 95 % CI=1.803-7.146,P<0.001), and the accumulated survival rate was significantly lower (P=0.001) in NSCLC patients with higher MCM2 expression than that of lower expression. MCM2 was independent prognostic factor of NSCLC patients (P=0.041). Conclusion MCM2 could reflect the reproductive activity of NSCLC and has some clinical significance for assessing the development and prognosis of NSCLC. MCM2 was a potential target for future treatment.

BACKGROUND: Experimental proof for the efficacy, safety, and immunological assessment is needed when minocycline is used for root canal disinfection.OBJECTIVE: To investigate the effect of minocycline for root canal disinfection on levels of interleukin-17 in apical exudates of periapical periodontitis and periapical exudate volume.METHODS: Sixteen patients with acute periapical periodontitis (16 teeth) scheduled for root canal therapy were enrolled and randomly divided into calcium hydroxide and minocycline groups, respectively, followed by root canal disinfection.One week after disinfection, periapical index, periapical exudate volume and interleukin-17 level were detected prior to the root canal filling. Another 16 patients with normal pulp vitality (16 teeth) scheduled for single root canal filling were enrolled as control group, in which periapical index, periapical exudate volume and interleukin-17 level were detected.RESULTS AND CONCLUSION: The periapical exudate volume and interleukin-17 level in the calcium hydroxide and minocycline groups were significantly higher than those in the control group (P < 0.05). The periapical index and interleukin-17 level in the calcium hydroxide and minocycline groups were decreased significantly at 1 week after root canal disinfection (P < 0.05), while there was no difference between these two experimental groups in the periapical index, periapical exudate volume and interleukin-17 level. To conclude, the use of minocycline significantly reduces interleukin-17 level and periapical exudate volume, and thus achieves effective outcomes in periapical disease.

Objective:To investigate the influence of p33~ING1b and wt-p53 on the biological activities of pancreatic cancer cells. Methods: Sense and anti-sense cDNAs of p33~ING1b were cloned into pcDNA3 eukaryotic expression vector separately, and the recombinant plasmids were solely or co-transfected with wt-p53 into pancreatic cell line SW1990. Flow cytometry was used to analyze the changes of cell cycle, Western blotting was used to evaluate the expression of p33~ING1b and p53 protein, and MG-P-MY staining was applied to observe cell apoptosis. Results: SW1990 cells solely transfected with sense p33~ING1b plasmid or co-transfected with wt-p53 and sense p33~ING1b plasmid expressed more p33~ING1b and had more apoptosis than the antisense p33~ING1b plasmid and mock transfected cells did (P

Background and purpose:With the recognition of prognostic factors and application of surgical- pathological staging,the treatment strategy of endometrial cancer has greatly changed.In this study,we investigated the treatment strategy,survival rate and prognostic factors of endometrial cancer.Methods:Five hundred and fifty-nine patients of endometrial cancer underwent primary surgical treatment from January 1996 to December 2006 in Cancer Hospital,Fudan University.The clinicopathologic parameters and prognosis were analyzed retrospectively.Results: Patients were followed up with the median period 36 months,25 cases experienced local relapse,24 cases were found to have distant metastases.The 3-year overall survival(OS)was 88.7%,3-year and 5-year disease free survival(DFS) was 82.7% and 80.9%.The 3-year overall survival(OS)were 95.5% in stageⅠ,95.3% in stageⅡ,75.9% in stageⅢand 32.4% in stageⅣrespectively(P