1.Effects of Caulis Sinomenii and sinomenine on morphine-induced place preference and brain histamine level in mice.
Zhi-xian MO ; Sheng-li AN ; Ji-yin ZHOU
Journal of Southern Medical University 2006;26(12):1709-1713
OBJECTIVETo evaluate the effects of Caulis Sinomenii and sinomenine on conditioned place preference (CPP) induced by morphine and brain histamine level in mice.
METHODSSixty mice were randomized into 6 equal groups and morphine (Mor) was injected subcutaneously (9 mg/kg) for 6 consecutive days to induce CPP using a shuttle box. Since the 4th day of training, the mice in 5 of the groups were treated for 3 consecutive days with Caulis Sinomenii (10 g/kg), sinomenine (60 mg/kg), diphenhydramine (30 mg/kg), CP48/80 (5 mg/kg) and L-histidine (750 mg/kg) in addition to morphine (9 mg/kg) treatment, respectively, leaving the other group with exclusive morphine treatment. Another 10 mice received saline injection to serve as saline control group. The content of histamine (HA) in the mouse brain was measured by fluorospectrophotometry.
RESULTSIn morphine group, the mice showed significantly extended stay in morphine-paired compartment whose HA content in the brain was markedly increased (P<0.01). Treatment with Caulis Sinomenii and sinomenine resulted in significantly reduced time of stay in morphine-paired compartment and brain HA level (P<0.01).
CONCLUSIONCPP induced by morphine in mice is associated with increased HA level in the brain. Caulis Sinomenii and sinomenine can suppress the acquisition of place preference induced by morphine and modulate HA level in the central nervous system in morphine-dependent mice.
Animals ; Arginine ; pharmacology ; Brain ; drug effects ; metabolism ; Conditioning, Operant ; drug effects ; physiology ; Diphenhydramine ; pharmacology ; Histamine ; metabolism ; Male ; Mice ; Morphinans ; pharmacology ; Morphine ; toxicity ; Morphine Dependence ; etiology ; physiopathology ; Motor Activity ; drug effects ; Random Allocation ; Sinomenium ; chemistry
2.The measurement of proportion and function of regulatory T cells in unexplained recurrent spontaneous abortion
Yu-Sheng LIU ; Shu-Yun ZHAO ; Jing-Juan JI ; Zhi-Min ZHAI ; Xian- TONG ; Li-Hua LUO ; Hong-Bing LUAN ;
Chinese Journal of Obstetrics and Gynecology 2001;0(03):-
Objective To investigate the proportion and function of CD_4~+ CD_(25)~+ regulatory T cells (CD_4~+ CD_(25)~+ Tr)in unexplained recurrent spontaneous abortion(URSA).Methods(1)Proportion measurement:the proportion of CD_4~+ CD_(25)~+ Tr cells in peripheral blood was measured by double-label flow cytometric analysis.The samples were taken from 15 URSA women,15 normal non-pregnancy women and 13 normal pregnancy women.(2)Function measurement:CD_4~+ CD_(25)~+ Tr ceils and CD_4~+ CD_(25)~+ T ce]ls were extracted from peripheral blood lymphocytes by the microbeads separation.The purity of CD_4~+ CD_(25)~+ Tr cells and CD_4~+ CD_(25)~+ T cells was measured by flow cytometry.The growth inhibitory effect of CD_4~+ CD_(25)~+ Tr cells on CD_4~+ CD_(25)~+ T cells was assessed in vitro.Results The proportion of CD_4~+ CD_(25)~+ Tr cells was decreased significantly in URSA women(6.9?1.8)% than that in normal non-pregnancy women[(10.8?1.1)%] (P0.05).Conclusion The results suggest that decrease in proportion and function of CD_4~+ CD_(25)~+ Tr cells may be associated with URSA.
3.Research on the influence of 12-week basic load resistance training on the physical fitness of flight students in an aviation school.
Zhe JI ; Hai-Tao ZHOU ; Zhi-Kang ZOU ; Xian GUO ; Xin ZHANG ; Hui CAO ; Zhi-Dong JIANG ; Xiang REN ; An-Li WANG ; Jian-Min CAO
Chinese Journal of Applied Physiology 2021;37(5):519-522
4.Cancer Risk Assessment among Medical X-ray Workers in China
Ji-Xian WANG ; Liang-An ZHANG ; Ben-Xiao LI ; Yong-Cheng ZHAO ; Zhi-Wei GAO ; Zhi-Quan WANG ; Jing-Yuan ZHANG
Acta Academiae Medicinae Sinicae 2001;23(1):65-68
Cancer incidence(1950 ~ 1995) among 27 011 medical X-ray workers in comparison with 25 782non X-ray medical specialists between 1950 and 1980 in China was investigated. The average cumulative dose received by the X-ray workers also reconstructed by retrospective dosimetry methods. Significant cancer risk was seen among medical X-ray workers(RR = 1.2). Significantly elevated risks were found in leukemia, cancer of skin, female breast, lung, liver, bladder and esophagus, the RRs were 2. 2, 4. 1,1.3, 1.2, 1.2, 1.8 and 2. 7 respectively. The patterns of cancer risk were associated with years since beginning of X-ray work, age and calendar year of initial employment and cumulative dose suggest that the risks of leukemia, skin cancer and female breast cancer, possibly thyroid cancer were related to occupational exposure to X-rays. A significant cancer risk could be induced by prolonged exposure to low dose ionizing radiation when the cumulative dose reached a certain level.
5.Relationship between DNA methylation and expressions of p57kip2 in hepatocellular carcinoma.
Ji-zhi ZHAO ; Zong-ji ZHANG ; Li-juan SHEN ; Ruo-chuan CHENG ; Hua-xian ZHANG ; Zhong-yi QIAN
Chinese Journal of Hepatology 2009;17(9):703-704
Biomarkers, Tumor
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metabolism
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Carcinoma, Hepatocellular
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genetics
;
metabolism
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CpG Islands
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Cyclin-Dependent Kinase Inhibitor p57
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genetics
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metabolism
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DNA Methylation
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Gene Expression Regulation, Neoplastic
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Humans
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In Situ Hybridization
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Liver
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metabolism
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Liver Neoplasms
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genetics
;
metabolism
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Polymerase Chain Reaction
;
methods
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Promoter Regions, Genetic
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RNA, Messenger
;
genetics
;
metabolism
6.A survey of the resources of medicinal plants in Luoding City.
Wen-qian CHEN ; Ji MA ; Wei TANG ; Zhi-xian MO
Journal of Southern Medical University 2010;30(10):2237-2241
OBJECTIVETo investigate the common resource of Chinese herbal medicine in western of Luoding City, Guangdong Province, and propose pertinent suggestions concerning the exploitation,utilization and conservation of the medicinal resources.
METHODSWith plant taxonomy method, we selected the JiaYi town as the center for local common Chinese herbal medicine resources, ecological environment and non-governmental investigation of medicinal.
RESULTSThere were 123 species of medicinal plants in Jiayi Town, including pteridophyte 11 species, gymnosperm 5 species, dicotyledon 97 species,and monocotyledon 12 species.
CONCLUSIONThis region has an excellent ecological environment and forest plant communities preserved relatively intact suitable for the growth of Lingnan Chinese herbal medicine, as well as a profound cultural background of folk medicine. The resources should be actively protected for further rational development and utilization.
China ; Conservation of Natural Resources ; Drugs, Chinese Herbal
7.The clinical classification method research of keloid.
Ji-Guang MA ; Jing-Long CAI ; Xian-Lei ZONG ; Jun-Cheng WU ; Zhen-Zhong LIU ; Su LIU ; Yu-Sheng SUN ; Zhi-Hua ZHANG
Chinese Journal of Plastic Surgery 2013;29(6):422-427
OBJECTIVETo explore the clinical classification method of keloids and providing a thread for the treatment of keloids.
METHODSTo summarize the 600 cases of keloid patients we accepted and diagnosed from November 2004 to October 2012, and filling in keloid patients information sheet, recording the keloids form by photographs, analyzing the treatment, putting forward the classification method of keloids in clinic.
RESULTSAccording to the position and quantity that keloids grow, the keloid patients are divided into four major categories:one in single site, one in each site, more than one in single site and more than one in each site; According to the area and thickness of keloids, the keloid single lesion is divided into four subclasses: type of small area and thin, type of small area and thick, type of large areas and thin,type of large areas and thick; According to the number of lesions, keloid multiple lesions is divided into two subgenera: isolated multiple and dispersion multiple, different kinds of keloids suit different methods of treatment.
CONCLUSIONThe clinical classification method of keloids can be used to provide thought for the treatment of keloids, and have a good application value.
Humans ; Keloid ; classification ; pathology ; therapy
8.An experimental study of immune function effect of rats irradiated with the complex field cure instrument.
Ming-hua ZHU ; Xian-zhi FU ; Yi ZENG ; De-quan WANG ; Ji-wei LU ; Chang-hong LI ; Zeng-shou WU
Chinese Journal of Medical Instrumentation 2002;26(5):339-341
The complex field cure instrument is a new medical instrument with which an experiment was carried out. Rats were continuously irradiated by the complex field for 90 days, with a day's total dose of 285.9 M.T.G. while other rats weren't irradiated for control group. The animals were respectively killed at 7d, 14d, 30d, 60d and 90d, and their blood samples were taken for cell and humoral immune analysis. The results show that values of lymphocyte transform rate, soluble receptor (SIL-2R), total hemolytic complement levels (CH50) and immunoglobulin (A.G.) after irradiation are more than those of the control group having proved that the instrument may improve immune function of rats.
Animals
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Female
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Immunoglobulin A
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blood
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Immunoglobulin G
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blood
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Immunoglobulins
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blood
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Lymphocyte Activation
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Lymphocyte Count
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Male
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Physical Therapy Modalities
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instrumentation
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Rats
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Rats, Sprague-Dawley
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Receptors, Interleukin-2
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blood
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Time Factors
9.Oral paroxetine for premature ejaculation: a randomized controlled study.
Zhi-yong GONG ; Tie-long TANG ; Shu CUI ; Ji-zhong WANG ; Xian-zhong DENG
National Journal of Andrology 2011;17(10):923-925
OBJECTIVETo validate the therapeutic efficacy of paroxetine in the treatment of premature ejaculation (PE).
METHODSEighty PE patients up to the inclusion criteria were equally randomized to an experimental and a control group. We observed all the patients for 4 weeks and recorded the baseline data on intravaginal ejaculatory latency time (IELT) and sexual satisfaction scores, followed by oral medication of paroxetine at 20 mg/d for the patients in the experimental group and placebo for the controls. Thirty days after the treatment, we again recorded IELT and sexual satisfaction scores of the patients.
RESULTSAfter the treatment, the experimental group showed significantly prolonged IELT ([5.75 +/- 1.24] min) and increased sexual satisfaction score (6.4 +/- 1.2) as compared with the baseline data ([0.89 +/- 0.21] min and [2.7 +/- 0.9]) (P < 0.01). The control group exhibited no significant differences before and after the medication either in the mean IELT or in sexual satisfaction scores ([1.06 +/- 0.28] min vs [0.97 +/- 0.18] min and 3.6 +/- 1.3 vs 3.1 +/- 1.1, P > 0.05).
CONCLUSIONOral medication of paroxetine at 20 mg/d for 30 days could improve IELT and sexual satisfaction in PE patients.
Adult ; Ejaculation ; Humans ; Male ; Paroxetine ; therapeutic use ; Serotonin Uptake Inhibitors ; therapeutic use ; Sexual Dysfunction, Physiological ; drug therapy ; Treatment Outcome ; Young Adult
10.Comparison of 99Tcm-DMSA renal cortical scintigraphy and ultrasonography in diagnosing acute pyelonephritis in children
Yi-wei, LI ; Qiang-ying, QIAN ; Rui-fang, ZHAO ; Zhi-ying, JI ; Xiao-mei, L(U) ; Ha, WU ; Xian-ying, CHENG ; Fan-lei, GU ; Xiao-fei, ZHAO
Chinese Journal of Nuclear Medicine 2010;30(5):336-338
Objective To compare the diagnostic value of renal ultrasound scan (RUS) and 99Tcmdimercaptosuccinic acid (DMSA) renal scintigraphy in children with acute pyelonephritis (APN). Methods In all, 165 children with initial clinical diagnosis of APN, aged from 1.5 months to 11 yrs ( median 20 months), were included in the study, all of which were examined with RUS and DMSA renal scientigraphy. The diagnosis with DMSA renal scientigraphy results was taken as the standard reference to evaluate the diagnostic sensitivity and specificity of RUS. Results Of 99 out of all 330 kidneys that were found abnormal on DMSA renal scientigraphy, 31 were abnormal on RUS. Of the rest normal kidneys on DMSA scans renal scientigraphy, 4 were abnormal on RUS. Thus diagnostic sensitivity of RUS for APN was 31.3%(31/99) and specificity was 98.3% (227/231). Conclusions Although RUS provides with high diagnostic specificity for children with APN, its low sensitivity may underestimate the clinical evaluation of APN.More often than not, 99Tcm-DMSA renal scientigraphy is a clinical necesscity for the definite RUS diagnosis.