China ; Hearing Tests ; Humans ; Infant, Newborn ; Neonatal Screening

Nowadays,research-based teaching is a teaching model,which has been accepted and applied universally.This paper deals with the characteristics of research-based teaching,which has been applied into systematic anatomy experiment teaching.

Hemodynamics ; Humans ; Hypertension, Portal ; physiopathology ; surgery ; Portal Vein ; physiopathology ; Portasystemic Shunt, Surgical ; methods

Antiretroviral Therapy, Highly Active ; CD4 Lymphocyte Count ; HIV Infections ; drug therapy ; immunology ; Humans

Wilson’s disease is an autosomal recessive disorder of copper metabolism. The resultant accumulation of copper primarily damages the liver and brain, resulting in hepatic, neurological and psychiatric symptoms. There have been many recent studies advancing the understanding of Wilson’s disease in Asia. There are indications that the incidence of Wilson’s disease in parts of Asia may be relatively high. Many genetic studies have identifi ed various hot spots in theATP7B gene in a variety of the Asian populations. Screening of these hotspot mutations may thus be useful in confi rming the diagnosis. Despite the advances in treatment, lack of familiarity by the health care profession resulting in late diagnosis, and poor access to treatment particularly among those from the developing economies remain areas of major concern.

OBJECTIVE: To discuss the mechanism of dioscin against apoptosis of cardiomyocytes induced by hydrogen peroxide through Serum Pharmacologic Method. METHODS: The drug-containing serum [0.6 g (crude drug) · kg-1] was prepared by serum pharmacologic method. Cardiomyocytes from neonatal SD rats were cultured in Dulbecco Modified Eagle Medium (DMEM). The primary cultured cardiomyocytes were injured by hydrogen peroxide before giving the drug-containing serum with different concentrations. The cardiomyocyte viability was determined by MTT method. Morphological changes of cardiomyocytes were observed by fluorescence microscope after treating with the drug-containing serum at different concentrations. The anti-apoptotic effect of dioscin was indicated by detecting the activity of caspase-3. The protein expression of Bcl-2 and Bax were semi-quantified by Western-blot method after treating with the drug-containing serum. RESULTS: The cardiomyocyte viability was elevated (P < 0.01) after being treated with different concentrates of drug-containing serum (0.4, 0.8, 1.2 g (crude drug) · kg-1, prepared from the original drug-containing serum). Typical apoptotic features of the cardiomyocytes such as membrane blebbing, cell shrinkage and detachment, and nucleus condensation and fragmentation were dose-dependently improved after being treated with drug-containing serum. The activity of caspase-3 decreased with the increasing concentration of drug. Western blot approach showed that the Bcl-2 level increased meanwhile the Bax decreased. CONCLUSION: Dioscin could increase the viability of the primary cultured cardiomyocytes in hydrogen peroxide induced injury. It could also decrease the activity of caspase-3, improve nucleus condensation and fragmentation, increase Bcl-2 level and decrease the Bax level. Copyright 2012 by the Chinese Pharmaceutical Association.

Objective:To prepare magnetic poly D, L-lactide-co-glycolide oxymatrine nanoparticle(M-PLGA-OM-NP) and investigate its preventive effects against experimental liver fibrosis. Methods: The M-PLGA-OM-NP was prepared by multiple emulsion process and observed by transmission electron microscope(TEM). Dimethylnitrosamine (DMN)-induced liver fibrosis was established with mice. After intervention with M-PLGA-OM-NP, the expression of α-smooth muscle actin (a-SMA) in the livers of mice was detected by immunohistochemical assay and the score of liver fibrosis was determined by H-E staining and Van Gieson (VG) staining. Results: The prepared M-PLGA-OM-NP was in a regular sphere shape, with a mean diameter of 146.5 nm; the drug loading was 7.61% and the encapsulation ratio was 44.8%. The serum Alt level in the M-PLGA-OM-NP group was decreased compared with that in the model group. Light microscopy revealed that the fibrosis in the M-PLGA-OM-NP group was greatly improved compared with the model group and pure oxymatrine group. The expression of a-SMA, the marker of hepatic stellate cell activation, was obviously decreased in the M-PLGA-OM-NP group compared with that in the other groups.Conclusion: The prepared M-PLGA OM-NP under magnetic field can reinforce the preventive effect of oxymatrine against DMNinduced experimental liver fibrosis in mice. Conclusion: The prepared M-PLGA-OM-NP under magnetic field can reinforce the preventive effect of oxymatrine against DMNinduced experimental liver fibrosis in mice.

Antibodies, Antiphospholipid ; blood ; immunology ; Antiphospholipid Syndrome ; diagnosis ; etiology ; immunology ; Child, Preschool ; Diagnosis, Differential ; Humans ; Male ; Partial Thromboplastin Time

Objective To investigate the expression of serum tumor markers in pancreatic carcinoma and to assess the value of serum tumor markers in the diagnosis and prognosis of pancreatic carcinoma. Methods Five serum tumor markers including AFP, CEA, CA199, CA125 and CA242 were detected in 95 patients with pancreatic carcinoma by chemi-luminescence immunoassay (CLI) and enzyme linked immunosorbent assay (ELISA), 80 patients with other malignant tumor and 65 patients with benign disease served as controls. Five tumor markers in pancreatic carcinoma diagnosis for TNM staging were compared. The value of tumor markers for prognosis was analyzed. Results Among these 5 kinds of tumor marker, CA199, CAI25, CA242 were valuable in diagnosis of pancreatic carcinoma, and CA199 was the most valuable one, whose sensitivity and specificity were 76.2 % and 74.8 % respectively. CA199, CA125, CA242 were increased with progression of pancreatic carcinoma. Conclusion Serum CA199, CA125 and CA242 are the preferred tumor markers to be used in the diagnosis and prognosis of pancreatic carcinoma.