3.One case of acute intermittent porphyria.
Ping ZHOU ; Zhi-min REN ; Qiang GAO
Chinese Journal of Pediatrics 2004;42(7):531-531
6. Expression of protein kinase C-α and its clinical significance in non-small cell lung cancer
Tumor 2007;27(12):981-984
Objective: To study the clinical significance of expression of the protein kinase C-α PKC-α) in non-small cell lung cancer (NSCLC) tissues and four types of NSCLC cell lines. Methods: PKC-α protein expression was examined by immunohistochemical SP method using tissue microarray technology in 160 cases with tumor tissue including 91 cases at stage I, and 69 cases at stage II-III and 40 cases of adjacent tissues. The mRNA and protein expression of PKC-α in NSCLC cell lines (A 549, A 549-DDP, H460 and H1299) were examined by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting, respectively. Results: Expression of PKC-α protein was positive in 53.1% (85/ 160) of cancerous tissues and 7.5% (3/40) of adjacent tissues (P < 0.01). But the expression of PKC-α had no significant difference between different types of NSCLC (adenocarcinoma, squamous carcinoma, and bronchioalveolar carcinoma). The positive rate of PKC-α protein was not related with the gender and age of NSCLC patients, but related with TNM staging and lymph node metastasis of NSCLC especially adenocarcinoma (P < 0.05). The expression of PKC-α protein was related with the differentiation degree of adenocarcinoma (P <0. 05), but not with the differentiation degree of squamous carcinoma. PKC-α were expressed in all NSCLC cell lines (A 549, A 549-DDP, H 460, and H 1299) at mRNA and protein level. A 549-DDP cells had the highest expression level of PKC-α. Conclusions: There exists high expression of PKC-α protein in NSCLC tissues and cell lines. The expression of PKC-α protein may play a crucial role in invasion, metastasis and development of NSCLC especially in adenocarcinoma. There is significant difference in the mRNA and protein expression levels of PKC-α in different NSCLC cell lines.
7.Growth activity of fetal liver stem cells in polyanhydride-three-dimensional vector-glucan
Meili YU ; Zhi DU ; Zhengyan ZHU ; Yingtang GAO ; Qiang GAO
Chinese Journal of Tissue Engineering Research 2009;13(47):9302-9304
OBJECTIVE: To observe effects of polyanhydride-three-dimensional vector-glucan material on the fetal liver stem cell adhesion and proliferation.METHODS: The two-step collagenase perfusion digestion and bliquid percoll discontinuous density gradient centrifugation was used to isolate fetal liver stem cells. Fetal liver stem cells at the third passage were incubated on the polyanhydride-three-dimensional vector-glucan material. Inverted microscope was utilized to observe cell adhesion and growth status. Cell adherent rate, proliferation activity were calculated, and cell number was counted. Cell-vector was obtained for tissue section. Using hematoxylin-eosin staining, cell growth in the vector was observed under the optical microscope. At 7 days,immunofluorescence staining and flow cytometry were used to determine marker expression.RESULTS: Polyanhydride-three-dimensional vector-glucan promoted grow and adhesion of liver stem cells. There was the active function of the liver stem cells within carrier materials. In the three-dimensional surface and the internal culture, liver stem cell proliferation was sustained. After 10 days, the polyanhydride common culture-three-dimensional vector-glucan on stem cells was non-toxic, and human fetal liver stem cells could be attached to the polyanhydride-three-dimensional vector-glucan stent. The cell proliferation was better and dynamic sustained expression of markers. 7-days training received 19.7 percent increase in the number of cells.CONCLUSION: Polyanhydride-three-dimensional vector-glucan promotes the proliferation of liver stem cells, and liver stem cells can be used as the vector in liver tissue engineering.
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