Objective To explore the clinical value of respiratory index(RI) and oxygenation index(OI)dynamic monitoring in neonatal respiratory distress syndrome(NRDS) during mchanical ventilation.Methods According to prognosis,24 newborns with NRDS who had not be treated with pulmonary surfactant in NICU of Chengdu maternal and child health hospital from Dec.2006 to Nov.2007 were divided into 2 groups.There were 16 newborns in survival group and 8 newborns in death group.The changes of RI,OI,p(A-a)(O2) and pa(O2) were stu-died at 2,24,48 h after using of synchronized intermittent mandatory ventilation and before ventilation weaning.Results In the early periods,there were significant differences in RI,OI and p(A-a)(O2) between the survival group and the death group(t=2.47,2.62,3.01 Pa

Hemodynamics ; Humans ; Hypertension, Portal ; physiopathology ; surgery ; Portal Vein ; physiopathology ; Portasystemic Shunt, Surgical ; methods

Estrogen receptor(ER),an important transcription factor belonging to the nuclear receptor superfamily,comprises two subtypes ER?and ER?.Estrogen receptor is expressed in prostate cancer and ladder cancer and has a compacted relationship with them.In this review,we summarized the structure,distri- bution,function of different estrogen receptor subtypes and progress in study on relationship between different estrogen receptor subtypes and prostate or bladder cancer.

Objective: To detect the loss of heterozygosity (LOH) of ING1 gene microsatellite and the expression of p33ING1b protein in lung carcinoma and to investigate their association with the carcinogenesis of lung cancer. Methods: Seventy cases of fresh-frozen lung cancers and 217 cases formalin-fixed, paraffin-embedded specimens were examined. The LOH frequency of ING1 gene microsatellite was detected by polymerase chain reaction-single strand conformation polymorphisms (PCR-SSCP) method. The expression of p33ING1b protein was determined by immunohistochemical method and tissue microarray technology. Results: The total LOH frequency of four microsatellites of ING1 gene was 55.7% (39/70) in lung carcinomas. The LOH had higher frequency if it was closer to the ING1 locus. But the LOH frequency had no correlation with clinicopathological parameters. The total LOH frequency of p33ING1b protein was 47.0% (102/217) in lung carcinomas. The positivity of p33ING1b in squamous carcinoma was significantly higher than that in adenocarcinoma (AC), adenosquamous carcinoma (AdCa), and bronchioloalveolar carcinoma (BAC) (P <0.05), while there was no significant difference between AC, AdCa, and BAC. The expression of p33ING1b protein was not associated with the clinicopathological characteristics but a negative correlation with LOH frequency was observed (P <0.05). Conclusion: There existes higher frequency of low expression or deletion of ING1 protein in lung carcinoma tissues. The LOH of ING1 gene microsatellites also have a higher frequency. LOH is one of the important reasons for the abnormal expression of ING1 gene, which causes the down-regulation of ING1 gene and inactivation of ING1 protein. Finally ING looses its inhibitory effect on the growth of tumor cells and results in carcinogenesis of lung cancer.

Objective To establish a hydrogen peroxide-impaired model of rat cerebral microvascular endothelial cells in vitro, and observe the protective effect of serum containing Naoyian. Method Rat cerebral microvascular endothelial cells were treated with six concentrations of hydrogen peroxide at four time points. The optimum injury condition of hydrogen peroxide was determined by MTT chromatometry. Then the cultured cells pretreated with serum containing Naoyian and pyrrolidine dithiocarbamate(PDTC) were interfered with hydrogen peroxide in suitable concentration and time, and OD of the treated cells was measured by MTT chromatometry. Results The cultured cells were injured obviously by hydrogen peroxide in 0 125mmol/L for 30min. The OD of the cells pretreated with 5% serum containing Naoyian and 50?mol/L PDTC were significantly higher than that of the cells without pretreatment (P

Neonatal brain damage including hypoxia-ischemia encephalopathy(HIE) and intracranial haemorrhage,may cause adverse neurodevelopmental outcome such as cerebral palsy.Earlier diagnosis is very important for the treatment of brain damage.This review is to elucidate the relationship between imaging changes(CT,MRI and ultrasonography) and prognostic assessment,thus provide reference for clinicians,the objective is focus on imaging changes of HIE and intracranial haemorrhages,which are 3 of the most frequently reported cases.

Although talus fractures are not common among foot and ankle injuries, their treatment is difficult because of their complications and poor prognosis. They can be treated conservatively or surgically, with a variety of treatment protocols. At present, the main strategy of surgical treatment is to use strong and effective fixation to restore anatomic structure of the talus and preserve blood supply to the talus as much as possible so that deformity can be avoided and early healing be facilitated. This paper reviews the difficulties and current situation, and summarizes principles and the latest progress in clinical treatment of talus fractures, hoping to provide useful references for the treatment.

Amino Acid Metabolism, Inborn Errors ; diagnosis ; genetics ; therapy ; Brain ; diagnostic imaging ; metabolism ; pathology ; Brain Diseases, Metabolic ; diagnosis ; genetics ; therapy ; Child, Preschool ; Diagnosis, Differential ; Glutarates ; metabolism ; Glutaryl-CoA Dehydrogenase ; deficiency ; genetics ; Humans ; Infant ; Infant, Newborn ; Lysine ; metabolism ; Magnetic Resonance Imaging ; Multiple Acyl Coenzyme A Dehydrogenase Deficiency ; diagnosis ; genetics ; therapy ; Mutation ; Neonatal Screening ; methods ; Radiography

Comet Assay

Administration, Intranasal ; Safety ; Steroids ; administration & dosage ; adverse effects