3.Research Progress on Insect-resistant Genes From Plants
Hong-Mei CHEN ; Kun-Zhi LI ; Li-Mei CHEN ;
China Biotechnology 2006;0(11):-
Plant insect-resistantgenetic engineering hasopened a new way to prevent and cure against the insect pest incrop and wood productions. With the progresses instudies, many insect-resistantgeneswere cloned and characterized. The classification of most insect-resistantgenes from plant, the functionalmechanism and the applications of theplantinsect-resistantgenes, the problems and prospectsforusing the plant insect-resistant genes in genetic engineering was described.
4.Clinical observation of chemotherapy combined with yiqi xiaozheng granule in treating advanced malignant tumor.
Hong SUN ; Ping-ping LI ; Yan-zhi CHEN
Chinese Journal of Integrated Traditional and Western Medicine 2003;23(5):382-383
Antineoplastic Combined Chemotherapy Protocols
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therapeutic use
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Cisplatin
;
administration & dosage
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Drug Therapy, Combination
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Drugs, Chinese Herbal
;
therapeutic use
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Female
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Fluorouracil
;
administration & dosage
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Humans
;
Intestinal Neoplasms
;
drug therapy
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Lung Neoplasms
;
drug therapy
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Male
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Phytotherapy
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Tetrahydrofolates
;
administration & dosage
5.Efficacy of blood purification on acute trichloropropane poisoning.
Hong-Jun YANG ; Zhi CHEN ; Li-Dan GAO
Chinese Journal of Industrial Hygiene and Occupational Diseases 2010;28(1):56-57
Adult
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Female
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Hemofiltration
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Humans
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Hydrocarbons, Chlorinated
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poisoning
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Male
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Middle Aged
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Poisoning
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therapy
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Treatment Outcome
8.Studies on a selective cyclooxygenase-2 inhibitor celecoxib inhibiting growth of human hepatocellular cancer SMMC-7721 cells lines and inducing apoptosis
Guohao XIA ; Hong CHEN ; Zhi WANG ; Liangxi PAN ; Jifeng FENG
China Oncology 2006;0(09):-
Background and purpose:The incidence of hepatoma is high. The outcome of treatment on hepatoma is poor.So we investigated the effect and mechanism of a selective cyclooxygenase-2 inhibitor celecoxib on the proliferation and apoptosis of SMMC-7721 hepatoma cell line. Methods:MTT assay was used to study the inhibitive effect of celecoxib on the growth of SMMC-7721 hepatoma cell. The effect of celecoxib on cell cycle and apoptosis on cells was studied by flow cytometry(FCM).Transmission electron microscopy (TEM) was used to display the morphological change of the SMMC-7721 hepatoma cell . The biochemical character of apoptosis was viewed on the agarose gel electrophoresis.The expression of bax gene and bcl-2 gene were measured by immunohistochemistry.Results:The SMMC-7721 cells were cultured in media that contained 25,50,75,100 ?mol/L celecoxib,by means of MTT, the inhibition rate was(15?3)%,(34.6?2.4)%,56.8?1.0)%,(86.2?0.4)% respectively after 24 hours; but the inhibition rate was (33.4?0.7)%,(66.7?1.8)%,(76.1?2.4)%,(97.3?0.8)% respectively after 48 hours(P
9.Detection of Legionella Pneumophila in Low Respiratory Tract Infection in Children Less Than 5 Years Old
guo-hong, ZHU ; ying-shuo, WANG ; zhi-min, CHEN
Journal of Applied Clinical Pediatrics 2006;0(23):-
Objective To study the present situation of legionella pneumophila(Lp) infection of low respiratory tract infection in children less than 5 years old.Methods Three hundred hospitalized patients with the diagnosis of low respiratory tract infection were enrolled in the study,191 males,109 females,aged 27 d to 5 years old,course from 1 d to 6 months.Serum antibody(IgM,IgG) of Lp serogroup 1-7 was detected by enzyme-linked immunosorbent assay(ELISA).Results In the patients,17 cases were positive,and the positive rate was 5.67%.Of the 17 children,the males(n=11) and females(n=6) did not significantly influence the positive rate(5.76% vs 5.50%).Four of them were between 27 days and 1 year old;7 were 1 to 3 years old,6 were 3 to 5 years old.The positive rates were significantly different among the 3 groups(2.53% vs 7.37% vs 12.77%) and tendentially increased following the increase of age(?2=7.8164 P
10.Application of combination transfer pump to continuous blood purification
Hongjun YANG ; Zhi CHEN ; Lidan GAO ; Hong ZHANG ; Lu KANG
Chinese Medical Equipment Journal 1989;0(04):-
Objective To evaluate the effect of combination transfer pump and single blood pump to 32 cases of renal failure patient who need blood purification therapy.Methods The combination transfer pump and single blood pump were used.According to the clinical requirement,the parameters of transfer pump were set such as therapy time,replacement liquid flow rate,dialysate flow rate,temperature of warmer and filtrate flow rate,etc.Results 32 patients with varying degrees of illness have been alleviated.Patients' acute left heart failure,water and electrolyte turbulences,acid intoxication and azotemia caused by liquid retention were corrected,which ensured a role for further treatment.Conclusion It is the functions of adjustable time and transfusion volume that are used in the combination transfer pump,the task of which is accomplished by several transfer pumps together.This method is easy to implement and can meet the clinical requirement largely without special CBP instrument.