Objective:To prepare renal cell carcinoma-dendritic cell fusion vaccines,and observe the biological characteristics of the fusion cells,to provide theoretical basis and experimental data for further research of DC based cancer vaccines. Methods:The renal cell carcinoma and dendritic cells were fused by using polyethylene glycol(PEG). The growth curve of fusion cells,morphological characteristics,expression of costimulation molecules,abilities to amplify T lymphocytes and grow into new tumors were detected. Results:Renal cell carcinoma and dendritic cells could be fused effectively by PEG.The fusion cells had weak proliferation abilities and were unable to grow into new tumors in immunol-deficiency animals,but the costimulation molecules expression such as CD86 and HLA-DR and the abilities to irritate the proliferation of T lymphocytes were remarkably increased than that of parental control groups(P

Social capital is an invisible capital form that promotes the efficient allocation and utilization of resources.It plays an active role in the prevention and control of chronic diseases.The purpose of this study is to investigate the effect of social capital on the three levels prevention of stroke by means of document retrieval.Social capital is associated with both morbidity and mortality of stroke.Social participation and social support at a high level can promote the rehabilitation of stroke patients,and have a beneficial effect on improving the quality of life and the ability of daily life.Social capital is correlated with stroke related behavioral factors,such as smoking,drinking,obesity,diet and physical activity.People with high social capital stock are more likely to choose healthy behavior patterns,thus reducing the risk of stroke.We should take full account of the social capital factors,and make more comprehensive and effective completion of the three levels of stroke prevention.

Health problems caused by age-related hearing loss can not be ignored, and elderly hearing screening that aimed to detect early and intervene early is of importance. As one of the main screening tools, elderly hearing screening scales are rarely used in China, so the paper mainly reviewed the development and application of hearing screening scales for elderly and provided a reference for future research and clinical application.

Objective: To examine the change of body weight (BW) and blood pressure (BP) in obese rats, clarify relationships between BP and BW and other factors. Methods: Male Spraque-Dawley rats were fed either with normal diet (ND) or high calorie diet (HC) for 20 weeks. BW and BP of tail artery were observed biweekly and tetraweekly respectively; serum leptin and fasting insulin (FINS) were detected by enzyme-linked immunoadsordent assay (ELISA) and radioimmunoassay (RIA) respectively. Fasting plasma glucose (FPG) and free fatty acid(FFA) were measured by conventional means. Results: BW, abdominal fat weight (AFW), ratio of abdominal fat weight to body weight (RF/W), systolic blood pressure (SBP), diastolic blood pressure (DBP), serum levels of leptin and FINS, FPG, FFA increased in the HD group after 20 weeks diet intervention (P<0.05 or P<0.01). SBP was strongly correlated with BW, leptin, FINS and FFA (P<0.05), DBP was correlated with FFA (r=0.47, P<0.05). In addition, leptin was positively correlated with BW, AFW, RF/W, FINS and FFA (P<0.05 or P<0.01). Conclusion: In this study of high calorie-diet induced rats, the gain of BW is accompanied by increased BP. The obese rats have hyperleptinemia, hyperinsulinemia, hyperglycemia and dyslipidemia which may have important effects on the development of obesity-related hypertension. R F/W is the key factor in which affect serum leptin level.

ATM: To investigate the relationship between the oculus dominans and the eye with higher diopter among myopes, and to study the difference between the Worth 4 dot test and hole-in-the-card test.
METHODS: Totally 125 cases were divided into two groups as physiogenic anisometropic group and pathological anisomitropic group according to the degree of binoculus anisometropy. Worth 4 dot test and hole-in-the-card test were used to distinguish oculus dominans respectively based on medical optometry.
RESULTS: In physiogenic anisometropic group, the oculus dominans and the eye with higher diopter among myopes had significant relationship by hole-in-the-card test (Z = -4. 057, P<0. 01) and Worth 4 dot test (Z=-3. 558, P<0. 01). However, in pathological group, No statistical significance was between two tests (P>0. 05); distinguish the oculus dominans had no statistically significant difference between the Worth 4 dot test and hole-in-the-card test (n=125, P>0. 05).
CONCLUSION:The oculus dominans is determined by gene or formed during juvenile period, then myopia anisometropic may affect the choice of that. The Worth 4 dot test has some reference significance for observing oculus dominans.

Gas Chromatography-Mass Spectrometry ; methods ; Humans ; Malathion ; metabolism ; urine

Aldehydes ; chemistry ; pharmacology ; Chromatography, High Pressure Liquid ; DNA Adducts

Objective To evaluate the efficacy of erlotinib on advanced NSCLC, and observe its adverse events. Methods An open labeled, expanded access program (EAP) was conducted on 19 pathologically confirmed advanced NSCLC patients who had received at least one regimen chemotherapy.Erlotinib (150 mg) was orally administered daily till disease progression or intolerable adverse events developed.The efficacy was evaluated according to RECIST criteria; the adverse events were evaluated according to NCI criteria.Results In 19 patients, the objective response rate was 21.1% (4/19), and the disease control rate was 84.2 % (16/19); the median progression-free survival time was 7.5 months (3-36 months), and the median survival time was 15.9 months (9-39 months).Adverse events were generally mild (grade Ⅰ or Ⅱ ), including skin rash (84.2 %) and diarrhea (57.9 %). One (5.3 %) patient developed grade Ⅲ elevation of serum glutamate pyruvate transaminase.No grade Ⅳ drug-related adverse event occurred.Conclusion Erlotinib is effective and safe for locally advanced or metastatic NSCLC patients who have failed previous chemotherapy.

The clinical therapeutic effects of Zhenerkang for 99 cases of children with hypoferric anemia and physique dysplasia were studied in present paper. For the anemia children two mouths of treatment made the Hb content raise by an average of 21.60g/L and the blood concentrations of metallic elements such as Fe,Ca,Zn,Cu,Se,Ge,etc elevate obviously. The drug was more effective for children with hypoferric anemia as compared with the control (Pearl powder).

Objective Lateral dislocation of the patella pulled laterally from the trochlea and across the lateral femoral condyle,was often a transient phenomenon with spontaneous reduction,diagnosis based on the results of physical examination and clinical history was difficult.The present was in an effort to explore the mechanism and definition of acute patellofemoral dislocation as well as assessment with MRI of the injury.Methods From May2001to July2002,34patients aging from11to25years with18males and16females,had a history of knee joint,28of which were experienced a twisting injury with the knee in flexion and internal rotation of femur on a fixed foot.Sagital,coronal and transverse MRI were performed with T 1 WI,T 2 WI,STIR and TSHIRT sequences.The manifestations of MRI were analyzed retrospectively,and clinical data,X-ray films,CT scan and the findings of arthroscopy were studied as well.Results All of34cases injured were found the signal intensity abnormalities of subchondral bone in the inferior medial part of patella and the anteri-or lateral part of lateral femoral condyle,which suggested that bone was injured.MRI findings of cartilage fis-sure at medial patella were found in20cases and injury of the patella retinaculum was seen at MRI in27cas-es.MRI findings revealed joint effusion in all patients,and lateral subluxation of the patella was seen at MRI in23cases.Conclusion The resultant jury on MRI findings of acute patellofemoral joint dislocation caused by a-cute impingent of patellar medial facet to lateral femoral chondyle at the various degree of flexion and rotation of the knee include the osteochondral fracture in the inferior medial part of patella,the anterior lateral part of lateral femoral condyle,the subluxation or dislocation of patella,the injury of medial patella retinaculum and the joint effusion.MRI could be helpful for the diagnosis of the disease,and demonstrate the location and ex-tent of injury on the different compartments of the involved knee.