Objective: To provide the scientific evidence for molecular identification and phylogenetic evolution by analyzing internal transcribed spacer (ITS) sequences in 14 different germplasms from Dioscoreae Rhizoma. Methods: The ITS regions were cloned by PCR amplification and sequenced bi-directionally. The ITS sequences were aligned using Clustal X software (version 1.83), the genetic distances were calculated using Mega software (version 4.1), and the phylogenetic trees were constructed through the Neighbor-joining (NJ) and maximum parsimony (MP) methods. Results: The length of ITS sequences obtained ranged from 558 to 594 bp, of which ITS1 was from 141 to 165 bp and ITS2 was from 146 to 158 bp. There were a lot of transitions and transversions in ITS sequences, and the transition/transversion ratio among 14 different germplasms was 5.347. Both in ITS1 and ITS2, 102 variable sites were further observed. The kimura 2-parameter (K2-P) genetic distance among 14 different germplasms ranged form 0 to 0.5172. The phylogenetic trees suggested that there were close genetic relationship among Dioscorea opposita, D. persimilis, and D. japonica, they grouped closely into Clade I; Furthermore, D. alata and D. fordii also shared a closer genetic relationship comprised another clade (Clade II). Conclusion: The phylogenetic analyses based on ITS sequences can present a foundation for clearing the evolution of germplasm resources in Dioscoreae Rhizoma; The variable bases can also provide reliable molecular evidences for identifying different genotypic germplasms of Dioscoreae Rhizoma.

It is a common phenomenon that medical research is out of line with clinical work at present. In fact, there is intrinsic interrelation between clinical work and research. Thinking and exploration at clinics are part of the research, and any research based on chnical work not only has great value, but also receives strong support from the state. However, to translate clinical research into clinical practice is difficult, which requires evidence-based methodology, a knowledgable team with persistence andtechnologies, et al.

OBJECTIVE: To investigate the genetic diversity of yam resources in China and provide reliable molecular evidences for cultivar identification and genetic relationship. METHODS: Sequence-related amplified polymorphism (SRAP) was applied to detect the genetic diversity of 21 yam cultivars from eight cultivated populations, Popgene software (version 1.32) was used to calculate genetic parameters, UPGMA clustering was carried out using Mega software (version 4.1), and principal component analysis was performed with the help of Ntsys-pc (Version 2.1) program. RESULTS: Three hundreds and nine bands were amplified by 20 SRAP primer combinations, of which 289 bands were polymorphic. The Nei's gene diversity index (H=0.2881), Shannon's information index (I=0.4416) and total genetic diversity (Ht=0.2891) revealed a relatively high genetic diversity level among yam populations. Within populations, HNWX population showed the highest genetic diversity (PPB= 35.92%), whereas the lowest diversity (PPB=0) was observed in FJSM and ZJRG populations. The genetic differentiation coefficient (Gst=0.8658) and genetic flow (Nm=0.0075) indicated that the genetic diversity among populations was higher than that of within populations. A well-separated groups coupling with cultivated species was formed according to the genetic distance (GD) ranging from 0.0498-0.4879 of the investigated populations. Twenty-one investigated cultivars could be distinguished and be effectively grouped into four origins, i.e., Dioscorea opposita Thunb., Dioscorea alata Linn., Dioscorea persimilis Prain et Burkill. and Dioscorea fordii Prain et Burkill.. CONCLUSION: The genetic diversity level of yam resources is high, and relatively high genetic variation exists among four yam species. SRAP marker is an effective method to identify and classify numerous yam cultivars.

Child ; Child, Preschool ; Female ; Humans ; Infant ; Male ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; physiopathology ; psychology ; Quality of Life ; psychology ; Survivors ; psychology

Child ; Humans ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; therapy

Adolescent ; Humans ; Precursor Cell Lymphoblastic Leukemia-Lymphoma

Objective To investigate the effect of radical surgical excision of total cyst for hepatic cystic echinoccosis. Methods During six years,86 patients with hepatic cystic echinococcosis were treated by radical surgical excision of the total cyst.Hospital stay,abdominal drainage time,residual cavity drainage,accumulated liquid in residual cavity,residual cavity infection and recurrence were compared with that in 90 cases receiving internal capsule extirpation. Results Radical surgical excision of total cyst for hepatic cystic echinococcosis is safe and very effective,the average hospital stay was significant shorter(P＜0.001)and there was no significant complications such as billiay fistula,residual cavity infection,accumulated liquid of residual cavity and recurrence of hydatid cyst. Conclusion Radical surgical excision of total cyst is an ideal and new therapeutic method for the treatment of hepatic cystic eehinococcosis.

By the breeding model of physics mutagenests (60Co,UV light) and re-strong in the body of insects, We obtained a Bt strain Bt99985 with high insecticidal activity. The shake flask and 40t tank fermentation tests showed that, this strain showed special activity against Spodoptera exjgua, Comparing with original Bt strain: 93005, CK strain: HD-1-580 and GC-91, the value of potency increased respectively by 429%, 655% and 114% in shake flask test. Comparing with GC-91, the value of potency increased by 180% in 40t tank fermentation test.

Objective To investigate the method of establishing lymph node metastasis model for gastric cancer in nude mice.Methods Human poorly differentiated gastric cancer SGC-7901 ceils were cul- tured,inherited,amplified and collected in vitro,and were inoculated in nude mice subcu.taneously.After pas- saging six generations,the subcutaneous tumor was orthotopically implanted in nude mice.The mice were then sacrificed on the 9th week after implantation.Serum CEA density was detected using radio-immunity method. Orthotopically implanted tumors,remote organ metastases as well as lymph node metastasis were observed macrescopically and histologically.Results The successful rate of orthotopically transplantion of gastric can- cer was 100 %.The rate of perigastric lymph node metastases was 93.3 %.Local infiltration and remote metastasis were observed.The level of serum CEA in nude mice bearing tumor was significantly higher than that of normal nude mice(P

OBJECTIVETo study on characteristics and application of key acupoints in Elongated Needle Therapy.

METHODSProbe into method of determining key acupoints and their clinical application from characteristics of channels, characteristics of acupoints, position of diseases, pathogenesis, syndromes and other aspects.

CONCLUSIONEstablishment of the theory of key acupoints of enlongated needle therapy reflects the principle of "fewer but better" in acupoint selection of enlongated needle therapy, which can reduce pain of the patient and increase the therapeutic