3.Expression of ?-catenin in keratosic dermatosis and non-melanocytic skin tumors
Ai-fang, JIANG ; Min-zhi, YIN ; Zhi-rong, YAO
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(07):-
Objective To study the expression and significance of ?-catenin in keratosic dermatosis and non-melanocytic skin tumors. Methods ?-catenin expression was determined in 10 specimens of normal skin,10 cases of seborrheic keratosis(SK),26 cases of actinic keratosis(AK),17 cases of Bowen's disease(BD) and 35 cases of primary cutaneous squamous cell carcinoma(SCC) by immunohistochemical EnVision two-step staining method. Results The expression of ?-catenin was significantly higher in SK,AK,BD and SCC than in normal skin(P0.05),but that was significantly lower in SK and AK than in BD(P0.05). Conclusion The cytoplasmic and/or nuclear expression of ?-catenin may play a role in the progression from normal skin to keratosic dermatosis and non-melanocytic skin tumors.
5.Congenital central hypoventilation syndrome: report of a case.
Chinese Journal of Pediatrics 2005;43(8):636-637
Abnormalities, Multiple
;
physiopathology
;
Bronchoscopy
;
Diagnosis, Differential
;
Hirschsprung Disease
;
diagnosis
;
physiopathology
;
Humans
;
Hypoventilation
;
complications
;
physiopathology
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Infant
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Laryngoscopy
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Male
;
Respiratory Insufficiency
;
complications
;
diagnosis
;
physiopathology
;
Sleep Apnea, Central
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etiology
;
Syndrome
6.Clear cell renal carcinoma coexisting with primary renal lymphoma: report of a case.
Chinese Journal of Pathology 2012;41(10):701-702
Antigens, CD20
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metabolism
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CD79 Antigens
;
metabolism
;
Carcinoma, Neuroendocrine
;
metabolism
;
pathology
;
Carcinoma, Renal Cell
;
metabolism
;
pathology
;
Diagnosis, Differential
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Female
;
Follow-Up Studies
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Humans
;
Kidney Neoplasms
;
metabolism
;
pathology
;
Leukocyte Common Antigens
;
metabolism
;
Lymphoma, Large B-Cell, Diffuse
;
metabolism
;
pathology
;
Middle Aged
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Neoplasms, Multiple Primary
;
metabolism
;
pathology
;
Nephrectomy
;
PAX5 Transcription Factor
;
metabolism
;
Wilms Tumor
;
metabolism
;
pathology
7.Rapamycin for myeloid blast crisis in refractory chronic myeloid leukemia with imatinib-resistance.
Jing XIE ; Xiang ZHANG ; Bao-Zhi FANG
Chinese Journal of Hematology 2011;32(8):553-554
Adult
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Antibiotics, Antineoplastic
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therapeutic use
;
Benzamides
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Blast Crisis
;
drug therapy
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Drug Resistance, Neoplasm
;
Humans
;
Imatinib Mesylate
;
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
;
drug therapy
;
Male
;
Piperazines
;
pharmacology
;
Pyrimidines
;
pharmacology
;
Sirolimus
;
therapeutic use
8.Paroxysmal nonepileptic abnormal movement during sleep in children.
Chinese Journal of Pediatrics 2007;45(12):903-905
Child
;
Chorea
;
physiopathology
;
Dyskinesias
;
diagnosis
;
physiopathology
;
Humans
;
Polysomnography
;
methods
;
Sleep
;
physiology
9.A case report of genetic analysis in the OCRL1 gene in Lowe syndrome.
Fang JIANG ; Yan GAO ; Zhi-ying OU
Chinese Journal of Pediatrics 2012;50(9):708-709
Child
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Codon, Nonsense
;
genetics
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DNA Mutational Analysis
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DNA Primers
;
genetics
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Dent Disease
;
diagnosis
;
genetics
;
Exons
;
genetics
;
Humans
;
Infant
;
Male
;
Mutation
;
Oculocerebrorenal Syndrome
;
diagnosis
;
genetics
;
Phosphoric Monoester Hydrolases
;
genetics
;
Polymerase Chain Reaction
10.Construction of a full-length cDNA clone of a live attenuated vaccine strain against Japanese encephalitis virus and preliminary study of expressing exogenous gene.
Bing HU ; Shuang YANG ; Zhi-zheng FANG
Chinese Journal of Virology 2014;30(6):652-660
This study aimed to construct full-length cDNA clones of the Japanese encephalitis virus (JEV). SA14-14-2 strain and discuss the feasibility of constructing chimeric viruses for exogenous gene expression based on the JEV genetic skeleton. Long-fragment RT-PCR techniques were applied to amplify JEV cD-NAs, and two amplified fragments with corresponding restriction endonuclease sites at both ends were cloned into the pACYC184 vector sequentially. Using standard molecular techniques, the enhanced green fluorescent protein (EGFP) gene was inserted into the 3' non-coding region of JEV as a reporter gene. After in vitro transcription and transfection procedures, wild-type JEV and chimeric JEV that expressed the EGFP as the reporter gene were successfully rescued. The recovered viruses were characterized by RT-PCR, plaque assays, and direct fluorescence microscopy. After six serial passage generations, the stability of the recovered viruses were studied in terms of virus growth characteristics and structural gene expression. The results showed that cDNA clones of rJEV and rJEV-EGFP were successfully constructed and rescued in BHK-21 cells after in vitro transcription and transfection. Each generation of the recovered viruses was stable and the chimeric virus rJEV-EGFP could stably express EGFP. The findings of this study indicate that both rJEV and rJEV-EGFP could be constructed and rescued in BHK-21 cells, and the JEV SA14-14-2 strain could be obtained as a viral vector to express foreign genes.
Cloning, Molecular
;
DNA, Complementary
;
genetics
;
metabolism
;
Encephalitis Virus, Japanese
;
genetics
;
metabolism
;
Encephalitis, Japanese
;
virology
;
Gene Expression
;
Genetic Vectors
;
genetics
;
metabolism
;
Green Fluorescent Proteins
;
genetics
;
metabolism
;
Humans
;
Vaccines, Attenuated
;
genetics
;
metabolism
;
Viral Vaccines
;
genetics
;
metabolism