A simple and reliable procedure for the isolation of pure DNA from human fetal liver is described.A high pure and natural DNA with an average molecular weight of 4 ?106 can be easily obtained after the sample is treated with SDS and trypsin and fractionated by a sepharose 4B column.The product meets the requirement for general research work of molecular biology.

This paper is to report the results of the clinical application of the Shexiang hemostasis-removing plaster. 265 cases of dermatohemostasis including chilblain, erythema multiforme, erythema nodosum, and so on were treated locally with Shexiang hemostasis-removing plaster. 20 cases of dermatohemostasis were treated with Shexiang analgesic plaster and served as control.It was found the rates of marked improvement and improvement after the application of Shexiang hemostasis-removing plaster were 85.61% and 96.60%, while those after Shxiang analgesic plaster treatment were 65% and 90% respectively. It is bovious that Shexiang hemostasis-removing plaster is more effective in treating dermatohemostasis than Shexiang analgesic plaster. The difference between the 2 is statistically significant(P

Acute kidney injury(AKI) can lead to remote organ damage.The specific mechanism by which the AKI results in distant organ damage is yet to be determined.In animal models,it has become increasingly clear that AKI is not an isolated event but results in remote organ dysfunction involving the heart,lungs,liver,and brain.This review summarizes the effects of AKI on remote organs.

The zymograms of lactate dehydrogenase (LDH) and esterase (EST) isoenzymes in different physiologic state from two species of Anopheles mosquitoes were studied with polyacrylamide gel electro -phoresis. Some zymogram variation of LDH from single female mosquito was found after engorged blood meals for 48 h, that consisted of two main bands whose relative mobility (Rm) was different. The isozymograms of EST showed some similar characteristics of sibling which divided into three groups ESTⅠ, ESTⅡand ESTⅢ, respectively. The two species of mosquitoes could be differentiated from each other by the particular band in EST Ⅱ group of An. anthropophagus.

Coronal restoration of endodontically treated teeth may be viewed as one of the main parameters that influence the outcome of endodontic treatment. The purposes of restoring endodontically-treated teeth include preventing recontamination of the root canal system and periapical area, replacing the compromised dental hard tissue, restoring the coronal morphology and function, providing necessary strength for the restoration/tooth complex for functional stress, and avoiding crown and/or root fracture. This article reviewed recent researches on the restoration of endodontically treated teeth, provided evidence for clinical practice on topics as when to restore them, basic principles to be considered during treatment planning, and specific restoration options for both anterior and posterior teeth under different functional occulsal load conditions. Several issues should be taken into account during the decision making process, such as remaining tooth tissue, functional masticatory forces, comprehensive oral rehabilitation, and esthetic requirements.
Decision Making ; Dental Restoration, Permanent ; Humans ; Root Canal Therapy ; Tooth Crown ; Tooth, Nonvital

Asthma ; epidemiology ; prevention & control ; therapy ; Child ; China ; epidemiology ; Drug Therapy, Combination ; Humans ; Immunotherapy

Zebrafish has unique advantages over other animal models in the aspect of drug screens for active ingredients and gains more and more attentions in drug research and development recently. Thus, this article reviews the recent advance of zebrafish-based drug screens in Chinese traditional medicine (TCM) effective part research, monomer drug screening, activity evaluation of natural products, discovery of new uses for old drugs, and toxicity assessment in early-phase drug discovery.
Animals ; Drug Evaluation, Preclinical ; instrumentation ; methods ; Drugs, Chinese Herbal ; pharmacology ; Models, Animal ; Zebrafish ; growth & development

Toxicogenomics is a new undertaking in the pursuit of human genomics relevant to health risk from environmental toxicants and related stress, and also is a new scientific subdiscipline derived from a combination of the fields of toxicology and genomics. Microarray technique, i. e. gene-chip technique includes gene-chip development, pretreatment of detected samples, probe hybridization, detection data analysis. The microarray technique in toxicology mainly was applied to identification of potential hazardous substances, screening for mechanism of action, dose-response relationship assessment, identification of interaction among chemical mixtures, biomarker of exposure and biomarker of susceptibility. Microarray technique has been used to study biomarker for human bladder cancer, to study fingerprints of gene regulation associated with cadmium chloride, benzo(a) pyrene and trichloroethylene. fn this paper, a new automated and multi-applied molecular biology workstation, NanoChip biomolecular system was introduced .

Objective To investigate the optimum extraction of total flavonoids and baicalin in baical skullcap root. Methods Ultraviolet spectrophotometry was used to detect the content of total flavonoids, HPLC was used to detect the content of baicalin. With the test of extracting method, time, frequency, and adding water volume, the optimum extracting craft was determined. Results The best craft for extraction was:heating water for recirculation, setting extracting time for 1.5 h, extracting frequency for 3 times, and water volume for 8 times of baical skullcap root. Conclusion The optimized process for extraction are stable, reasonable and feasible.

Objective To compare the regulatory functions of Danhong and Salvia Ligustrazin on abnormal coagulation factors Ⅶ caused by ACS.Methods All cases were randomly divided into group A and B and a control group.Each group contains 60 patients.The control group was treated with conventional western therapies.On this basis,group A Was treated with Danhong injection and group B was treatedwith Salvia Ligustrazin injection.Compare the regulatory function of its group on abnormal level of coagulation factor Ⅶ caused by ACS.Results Group B showed better effects in its regulating abnormal level of coagulation factor Ⅶ as compaared to group A(χ~2=3.67,P＜0.05).Both groups did better than the control group in adjusting coagulation factors Ⅶ (P＜0.05).Conclusion Salvia ligustrazine has better effect in regulating coagulation factors Ⅶ than Dan Hong injection,which is better than conventional western medical therapy.