Objective Amniotic membrane as the carrier to culture the vascular endothelial cells was investigated in this study in order to explore whether the corneal endothelial cells superseded by the vascular endothelial cells is feasible on the account of directing a kind of the original method to handle the bullous keratopathy.Methods The vascular endothelial cells adhering to the sphagitides lumen of the experimental rabbits were digested and gained by means of the perfusion method with 0.25%tripsin plus 0.02%EDTA.Fresh amniotic membrane without the amniotic epithelial cells was cut into the square tissue about 1.5 cm?1.5 cm and spread evenly in the 24-well culture dish.Primary cultured cells were subcultured on the amniotic membrane.We would not transplant the vascular endothelial cells feeding on the amniotic membrane until cells is full of the whole amniotic membrane surface.One to two months after the operation,the change of corneal diaphaneity was observed.Results About 12 days since the cell transplantation was performed,the corneal transparency alteration between the experimental groups and the control one is different.The corneal buttons in the experimental group show the severe edema and opacity,and the anterior chamber couldn't be seen unclearly.But,10 days after the operation,the corneal oedema which begins to extenuate was judged through the indicatrix that the corneal edema in the experimental group has been recovering slowly,among which the anterior chamber tissue of 7 animals was visible through the implant.The corneal edema in the control groups intensified evidently,even,the part of these appeared the ulcerous necrosis as result of the corneal severely edema.There is the existence of difference between two groups(P

Objective To develop a aided mechanical appliance for rapid reconstruction of three-dimensional(3D) relationship of dentition model after scanning and evaluation of its accuracy.Methods The appliance was designed by forward engineering software and fabricated by a high precision computer numerical control(CNC) system.It contained upper and lower body,magnetic pedestal and three pillars.Nine 3 mm diameter hemispheres were distributed equally on the axial surface of each pedestal.Faro Edge 1.8m was used to directly obtain center of each hemisphere(contact method),defined as known center.A pair of die-stone standard dentition model were fixed in intercuspal position and then fixed on the magnetic pedestals with low expansion ratio plaster.Activity 880 dental scanner was used to scan casts after the plaster was completely set.In Geomagic 2012,the centers of each hemisphere were fitted and defined as scanning centers.Scanning centers were aligned to known centers by reference point system to finish the 3D reconstruction of the intercuspal occlusion for the dentition casts.An observation coordinate system was interactively established.The straight-line distances in the X(coronal),Y(saggital),and Z(vertical) between the remaining 6 pairs of center points derived from contact method and fitting method were measured respectively and analyzed using a paired t-test.Results The differences of the straight-line distances of the remaining 6 pairs of center points between the two methods were X:(-0.05±0.10) mm,Y:(0.02±0.06) mm,and Z:(0.01 ± 0.05) mm.The results of paired t-test showed no significant differences(P＞0.05).Conclusions The mechanical appliance can help to reconstruct 3D jaw relation by scanning single upper and lower dentition model with usual commercial available dental cast scanning system.