Objective:To investigate the effects of Penehyclidinehydrochloride(PHCD) on endotoxin-induced cerebral edema.Methods:one handred and five S-D male rats(200～220g)were randomly divided into 5 groups(n=21):groupC control;groupL LPS;group DL,DM and DH received intraperitoneal PHCD 0.05,0.15 or 0.45mg/kg 10min before lipopolysaccharide(LPS) administration.Cerebral edema was induced by internal carotid arterial LPS 150?g.Seven animals each group were decapitated at 4、6 and 12h after operation and their brains were immediately removed for determination of water content of brain and microscopic examination.Results:Administration of LPS caused severe brain damage and significantly increased water content of the brain.The LPS-induced changes were mitigated by pretreatment with different doses of PHCD in groupDM and DH,but there was no significant difference between the two groups.Conclusion:Pretreatment with PHCD can attenuate LPS-induced cerebral edema.

Objective:To study the factors affecting amplified fragment length polymorphism(AFLP) of Carthamus tinctorins L.genome,and to develop and optimize the AFLP reaction system,laying a foundation for Marker-assisted breeding of Carthamus tinctorins L..Methods: Cetyl-trimethyl-ammonium-bromide(CTAB) method was used to extract the genomic DNA of Carthamus tinctorins L.,whose concentration and purity were determined by UV spectrophotometry.The genomic DNA was digested by restricted enzyme and ligated with one-step method or two-step method so as to find out which one is more suitable.Then the products were prepared with different dilution multiples(5,10,15,20,25 and 30) for pre-amplification and with different concentrations of gradients(10,25,50,75,100,150 and 200)for selected amplification.Then they were subjected to PAGE electrophoresis and silver-staining after denaturing at 95℃ for 8 min.Results: The AFLP reaction system for Carthamus tinctorins L.genome included the following steps: the genomic DNA was precipitated with alcohol by CTAB method to avoid contamination by any inhibitors;the DNA was completely digested by MseⅠ and EcoRⅠ at 37℃ for 3 hours.Two-step method was superior to one-step method in this AFLP system.The digestion time was 3 hours at 37℃ and ligated at 16℃ over night,with Buffer 2(NEB Company) as the specific buffer.Furthermore,the optimal dilution was 25 folds for pre-amplification and 75 folds for selected amplification. In this reaction system,the strips in PAGE electrophoresis were clear and less decomposed.Conclusion: The AFLP reaction system in this experiment is suitable for silver-stained AFLP of Carthamus tinctorins L.analysis.

The incidence of bronchial asthma in children is increasing nowadays.More and more studies have showed that epigenetics plays an important role in the pathogenesis of asthma.One of them are histone modification(acetylation and deacetylation),which is catalyzed by histone acetyltransferase (HAT) and histone deacetylase (HDAC).HAT and HDAC can mediate asthma via causing Th1/Th2 imbalance,increased airway hyperresponsiveness,regulation of various inflammatory medium and so on.This paper reviews the HAT and HDAC mediated incidence of asthma and other possible mechanisms,the latest of glucocorticoid in the treatment of children with asthma epigenetic mechanisms.

Sulfur dioxide has been considered as a toxic gas.However,recently more and more studies have shown that sulfur dioxide can be generated endogenously in cardiovascular system and has broad physiologic effects.Studies demonstrate that sulfur dioxide plays an important role in the regulation in the pathophysiologic process of many cardiovascular diseases,such as hypertension,atherosclerosis.These findings suggest that sulfur dioxide might be a novel endogenous gaseous signaling molecule involved in the regulation of cardiovascular functions.

Since Epstein Barr virus was shown to encode microRNAs(miRNAs) in 2004, more than 470 miRNAs have been discovered in α-, β-, and γ-herpesviruses. MiRNAs are small non-coding RNA molecules and generally only have 18-25 nucleotides in length, which can regulate the expression of target genes by targeting its transcripts. Herpesvirus-encoded miRNAs not only target the key genes from latency to lytic replication, but also regulate various host cellular genes. Current data manifest that herpesvirus-encoded miRNAs can regulate viral latent infection and lytic replication, immune recognition, apoptosis, and tumorigenesis. The purpose of this paper is to summarize the targets and their fuction of hepesvirus-encoded miRNAs, in order to provide theoretical support for further analysis herpesviral pathogenesis.
Animals ; Herpesviridae ; genetics ; metabolism ; Herpesviridae Infections ; virology ; Humans ; MicroRNAs ; genetics ; metabolism ; RNA, Viral ; genetics ; metabolism

Entrepreneurship of the students in TCM colleges can help alleviate the pressure of employment and take advantages of TCM to benefit the public, deepen medical reform, promote the heritage and develop the TCM culture. The influence of the environment could be throughout the whole process of entrepreneurship. The entrepreneurial environment of the students from TCM colleges were analyzed in the following three factors, such as family environment, school environment, and social environment. Only these three environment work together could help entrepreneurship go well. Thus, it is important to change the traditional concept, develop education system of entrepreneurship, and improve the entrepreneurial support system.

OBJECTIVE: To analyze the characteristics of adverse drug reaction (ADR) induced by Levofloxacin (LVFX) injection and to promote rational use of drugs in the clinic. METHODS: 137 LVFX injection-induced ADR cases reported in our hospital during 2006～2009 were collected and analyzed statistically with Excel in respect of age, gender, medication and clinical manifestation, etc. RESULTS: ADR cases occurred nearly in all ages of people, and 32.85% were appeared in people over 60 years. Main clinical manifestations were lesion of skin and its appedants (62.04%) and gastrointestinal injury (16.06%). Most of them were mild and medium ADR. CONCLUSION: Great importance should be attached to the rational use of LVFX injection and ADR monitoring to guarantee the safety of patients.

Although reports on the diagnosis of CHD angina pec-toris are plenty, yet studies on nature and typing of Zheng on its two main types, spontaneous and overworked, of anginal attacks are scarce. During the period Jan. 1985 -July 1994. we treated 92 cases of spontaneous angina pectoris and 303 cases of overworked angina on the basis of TCM syndrome typing, in the hope of giving assistance to the correcttherapy of this disease.

OBJECTIVE:To study the effects of different extraction methods on the content of cholalic acid from Artificial Bezoar. METHODS: Artificial Bezoar from 3 different habitats was used to extract cholalic acid by hydrolization vs. hydrolization-free method, and the content of cholalic acid was determined by HPLC-ELSD(high performance liquid chromatography with evaporative light scattering detection). RESULTS: The content of cholalic acid in the hydrolyzed sample was higher than that in the non-hydrolyzed sample of the same batch. CONCLUSION: Through hydrolization, the binding cholalic acid in the artificial bezoar was transformed into free cholalic acid so that the content of cholalic acid was increased. Therefore, it is more reasonable to control the quality of Artificial Bezoar by taking the contents of total cholalic acid and free cholalic acid as indexes.