Objective: To examine the inflammatory indicators in patients with oral squamous cell carcinoma (OSCC), so as to provide insights into early screening and recognition of OSCC. Methods: A total of 86 OSCC patients, 61 patients with chronic periodontitis (CP) and 71 patients without the above diseases were selected from Peking University Hospital of Stomatology and were enrolled in the OSCC group, CP group and control group. Procalcitonin (PCT), serum amyloid A (SAA), C-reactive protein (CRP), white blood cell count (WBC), neutrophil percentage (NEUT%), neutrophil to lymphocyte ratio (NLR), lymphocyte to monocyte ratio (LMR), platelet count and lymphocyte ratio (PLR) were measured in peripheral blood. Receiver operating characteristic (ROC) curves were used to evaluate the effectiveness of the aforementioned inflammatory indicators in OSCC screening. Results: The OSCC group consisted of 46 males and 40 females, with a median age of 59.50 (interquartile range, 18.25) years; the CP group consisted of 32 males and 29 females, with a median age of 53.00 (interquartile range, 14.50) years; the control group consisted of 36 males and 35 females, with a median age of 54.00 (interquartile range, 16.00) years. Compared with the control group and the CP group, the PCT, SAA, CRP, WBC, NEUT%, NLR and PLR in the OSCC group were increased, and the LMR was decreased, and the difference was statistically significant (all P<0.05). The combined use of PCT, SAA and CRP had a good effect in distinguishing the OSCC group from the control group, with an area under the ROC curve (AUC) of 0.992, which was higher than the individual identification of the 8 indicators (all P<0.05). The combined use of PCT, WBC, and NLR also had a good effect in distinguishing the OSCC group from the CP group, with an AUC value of 0.841, which was higher than the individual identification of WBC, LMR and PLR (all P<0.05). Conclusion Compared with patients with CP, as well as patients without OSCC and CP, patients with OSCC exhibit significant differences in inflammatory indicator levels, which may facilitate early identification of OSCC.

ific primer pair S2-R2 can be used to detect S. schenckii in paraffin-embedded tissue.

Objective To evaluate the clinicophatholgic benefits and safty of antivirus therapy in patients with liver cirrhosis resulting from hepatitis B.Methods 80 patients with HBV-ralated liver cirrhosis were divided into three groups by the histopathology of liver:group of lamivudine treated with lamivudine 100 mg once daily;Adefovir group treated with Adefovir 10 mg once daily;control group treated with liver protective treatment only.Liver and renal function,PTA and HBV DNA were regularly measured.The Child push-Turotte sore and histopathology wag compared before and after treatment.All courses of treatment were 36 weeks.Results The scores of Child Pugh-Turotte sore in groups of lamivudine and Adefovir were lowered sinificantly (3.9 and 2.1 respectively),the load of HBV-DNA was decreased also[(4.1±0.9) copies/ml and(2.8±1.0) copies/ml],liver inflammmation decreased by more than 2 scores and liver fiber was improved by more than one score,with obviously significant difference(P<0.05) as compared with control group.Conclusion Patients with HBV-related cirrhosis treated with lamivudine and adefovir for antivims are improved and antivirus is important and safe to those during cirrhosis decompensation.

Objective To assess the efficacy of topical application of Ephedrine and furacilin nasal drops combined with tetracaine in reducing complications of pack removal after nasal surgery.Methods 81caseshaving un-dergone nasal septum surgery were randomly divided into the experimental group and control group: the former were treated with Ephedrine and furacilin nasal drops combined with tetracaine and the blank control group.The occur-rence of complications at removing nasal fillers after nasal surgery was assessed.Results The hemorrhage and pain in the experimental group were significantly less than those in the blank control group (p <0.05).Conclusion top-ical application of Ephedrine and furacilin nasal drops combined with tetracaineis an effective method for reducing the pains of pack removal after nasal surgery.

Objective:To study the protective effect of telmisartan on rats with renal failure and its mechanism. Methods:60 Wistar rats were chosen as study objective, and were divided into 4 groups randomly:15 in group A (sham operation group), 15 in group B (model group), 15 in group C (telmisartan group) and 15 in group D (telmisartan+GW9962 group). The difference of survival rate, blood-urine biochemical indexes, renal pathological change, and the expression level of PPARγ and nNOS were compared. Results:After 12 weeks, the survival rate of group A was 93.33%(14/15), that of group B was 46.67%(7/15), that of group C was 86.67%(13/15), that of group D was 60.00%(9/15), and the difference among 4 groups had statistical significance (P<0.05). After 1 week, the difference of Scr, that of BUN and that of 24h protein urine among 4 groups was not statistical significant (P>0.05);after 3 weeks, 6 weeks and 12 weeks, these difference was statistical significant (P<0.05). The difference of blood-urine biochemical indexes, that of renal pathological change, and that of the expression level of PPAR毭and nNOS was statistical significant (P<0.05). Conclusions:Telmisartan has protective effect on renal failure caused by 5/6 nephrectomy, which might be relative to the expression level of PPARγ and nNOS.

Objective:To study relativity between tumor metastasis and the level of immune competent cells from patients with malignant carcinoma.Methods:The percentage of NK cells and CTL cells and monocyte/macrophage cells were measured and analyzed in tumor tissue and peripheral blood from patients with malignant carcinoma by flow cytometry.Results:NK cells, CTL cells and monocyte/macrophage cells of tumor tissue and peripheral blood were lower than those of normal control groups ( P

Objective To study the relation between vascular endothelial growth factor(VEGF) and the degree of coronary artery stenosis as well as the formation of collateral circulation in patients with acute myocardial infarction (AMI). Methods Seventy-six cases of AMI had undertaken selective coronary angiography on the third to seventh day after admission and also at six months afterwards in order to define the degree of coronary artery stenosis and the presence of collateral circulation. A blood sample of 5 mL was taken in each patient before the first coronary angiography to test for the level of VEGF by enzyme linked immunosorbend assay. Results The level of VEGF in patients with coronary artery stenosis less than 50%, 50%~75% and greater than 75% were (97.6?17.3) ng/L, (241.6?28.9) ng/L and (391.7?48.4) ng/L respectively (P

Objective To compare the therapeutic effect and complication of immediate and selective interlocked intramedullary nailing in treatment of open tibial shaft fractures. Methods A total of 123 open tibial shaft fractures of 102 cases were treated with immediate fixation with interlocked intramedullary nailing after debridement (Group A) and with selective fixation with interlocking intramedullary nailing after debridement and closing the wound (Group B). Results The follow up for more than half a year showed that all fractures were healed, with excellent joint function of knee and ankle. Of all, 12 cases with skin necrosis and defect but without deep infection were healed after pedicle flap reconstruction. The result of Group A was much better than that of Group B in regard of total operation time, hospital stay, cost, amount of antibiotics used and partial weight bearing. No statistical difference in therapeutic effects and complications was found between two group. Conclusion Infection is not the contra-indication of interlocked intramedullary nailing in open tibial fractures. Immediate operation can be done after thorough debridement.

Objective To investigate the effects of NB-UVB on the expression of Gadd45α as well as cell apoptosis and cycle of human HaCaT keratinocytes.Methods Cultured HaCaT cells were exposed to various doses (100,200,400 mJ/cm2)of NB-UVB followed by an additional culture of 6,12 and 24 hours,respectively.Reverse transcription PCR and Western blot were performed to detect the mRNA and protein expression of Gadd45α respectively in HaCaT cells,cell counting kit 8 (CCK8)to measure the proliferation of cells,and flow cytometry to determine the cell cycle distribution of HaCaT cells before and after the exposure to NB-UVB.Results Gadd45α was expressed in HaCaT cells.After exposure to NB-UVB of the three doses,the mRNA and protein levels of Gadd45α increased at 6 hours and 12 hours,but declined at 24 hours,and significant changes were observed in HaCaT cells at the three time points after exposure to NB-UVB of the three doses (all P<0.05).The Gadd45α/β-actin mRNA ratio was 1.4360±0.6551.1.8633±0.0979,1.9266±0.1724 in HaCaT cells 12 hours after irradiation to NB-UVB of 100,200 and 400 mJ/cm2,respectively,significantly higher than that in unirradiated cells(0.6000±0.1276,all P<0.05).Also,increased Gadd45α/β-actin protein ratio was noted in HaCaT cells 12 hours after irradiation to NB-UVB of 100,200 and 400 mJ/cm2 compared with unirradiated cells (0.0773±0.0005,0.1936±0.0015,0.2373±0.0015 vs.0.0290±0.0010,all P<0.05).NB-UVB inhibited the proliferation of HaCaT cells in a time-and dose-dependent manner.Flow cytometry showed that irradiated HaCaT cells were blocked in G2 phase of the cell cycle.and the percentage of HaCaT cells in G2 phase was 13.53%±1.03%,17.77%±2.25%,30.03%±4.29%afler exposure to NB-UVB of 100,200 and 400 mJ/cm2,respectively,compared to 9.24%±0.97%in unirradiated cells (all P<0.05).Conclusions The expression of Gadd45α is increased in HaCaT cells after exposure to NB-UVB,and Gadd45α may be involved in the NB-UVB-induced suprression of cell proliferation of and cell cycle arrest in HaCaT cells.

A case of granular parakeratosis is reported. A 31-year-old woman presented with a 23-year history of pruritic erythema and erosion in the left axilla. On examination, there was a ring-like annular erythematous patch sized 8 cm×10 cm in the left axilla. Bright mauve, cone-shaped, millet-like papules were observed in the center of the lesion, some confluenced into plaques. Erythema was present in the pedlesional region along with mild erosion, exudation and small numbers of grain-sized pustules. Scar formed in some perilesional areas. No lesions were noted at any other intertriginous regions. Fungal microscopy of lesion secretions was negative. Histological examination of biopsy specimens from the center of the left axilla revealed psoriasiform hyperplasia of epidermis and thickened stratum comeum with hyperkeratosis and parakeratosis. Most cells in the stratum comenm retained nuclei and contained numerous basophilic granules. Granular layer could be noted under the parakeratotic cells with cytoplasm vacuolization of some cells. There was a perivascular, mixed inflammatory infiltration predominated by lymphocytes and hemangiectasis in the dermis. A diagnosis of granular parakeratosis was made.