In the treatment of 48 cases of menopause syndrome by moving cupping therapy along the pathways of the bladder meridian, Jiaji points (Ex-B 2) and pathway of the Governor Vessel, the results showed cure in 10 cases, remarkable effect in 27 cases, effect in 8 cases and failure in 3 cases, and the total effective rate in 93.7%. In comparison with before the treatments, all the symptoms were relieved (P＜0.05) after treatments, with insomnia, poor memory, depression and easy crying, warm sensation in the five body parts, aching and soft sensation in the low back and knee relieved more remarkably (P＜0.01).

Objective To evaluate CT findings of urinary obstruction caused by ureteral diseases.Methods CT findings in 157 cases with ureteral disease, proven surgically and pathologically were retrospectively reviewed.Results 27 cases of ureteral neoplasms, 12 casesof ureteritis, 10 of ureteral tuberculosis, 90 of ureteral stones, 3 of ureteral polyps, 3 of retroperitonium fibrosis and 12 cases ofretroperitonium or pelvic neoplasms involving ureter were included. Conclusion Spiral CT is of high accuracy in determining the natures and localizations of ureteral diseases.

Objective:To investigate the immune depression to donor-origin cells induced by the Mixed Chimera after sublethal whole body irradiation.Methods:Recipients in the experiment were Wistar and SD rat,and Wistar rats were selected as the donor.Donor and recipient rats were divided randomly into three groups.Recipient were conditioned with sublethal whole body irradiation (WBI).Group A was infused with bone marrow cells (BMC) of Wistar rats;group B infused with bone marrow mesenchymal stem cells (BM-MSCs)of Wistar rats;and group C with normal saline.Then they were administered cytoxan(CTX) by intraperitoneal injection.The mechanisms for immune depression were explored by performing mixed lymphocyte reaction (MLR).Results:The results showed that donor lymphoid chimeras could be found in the immune depression SD rats and chimerac cells in group A was more than in group B by FCM assay (P

This study was designed to determine the impact of chrysoeriol on proliferation and cell cycle progression in the human multiple myeloma cell lines RPMI 8226 and KM3, and its related molecular mechanisms. Chryseoriol was identified by using the phosphorylated AKT-specific cytoblot high throughput assay. CCK-8 assay was employed to examine the growth inhibition rate and IC(50) (48 h) in peripheral blood mononuclear cells (PBMNCs), RPMI 8226 and KM3 cells treated with chrysoeriol at various concentrations. Cells were labeled with 5-6-carboxyfluorescein diacetate succinimidyl ester (CFSE), and the proliferation dynamics was detected by flow cytometry and analyzed with ModFit software. The cell cycles of RPMI 8226 and KM3 cells were measured by flow cytometry when the IC(50) concentration of chrysoeriol was adopted. The alterations in cell-cycle related proteins (Cyclin B1, Cyclin D1, p21) and proteins in PI3K-AKT-mTOR pathway were determined by Western blot analysis. The results showed the proliferation of multiple myeloma cells was significantly inhibited by chrysoeriol, resulting in cell cycle arrest in G(2)/M phase. Chrysoeriol could significantly reduce the expression of p-AKT (s473) and p-4eBP1 (t37/46) protein, meanwhile enhanced Cyclin B1 and p21 protein expression. Similar effects were not observed in PBMNCs from normal donors. It was concluded that chrysoeriol was a selective PI3K-AKT-mTOR pathway inhibitor. It restrained the proliferation of human multiple myeloma cells, but didn't affect proliferation of PBMNCs from normal donors. It might exhibit the cell cycle regulatory effect via the inhibition of PI3K-AKT-mTOR signal pathway.

OBJECTIVETo explore an effective method for treatment of senile habitual constipation.

METHODSSixty-four cases were randomly divided into a treatment group treated with moving cupping at Hechelu plus Shenque (CV 8) Bazhenxue moxibustion, and a control group with oral administration of Tab phenolphthaleinum, 32 cases in each group. Improvement of constipation in the patients were observed and compared.

RESULTSThe short-term (14 days) clinical cured-markedly effective rate was 84.4% in the treatment group, which was significantly better than 59.4% of the control group (P < 0.05), and there was a significant difference between the two groups in the long-term (2 months) therapeutic effect (P < 0. 01).

CONCLUSIONMoving cupping at the Hechelu combined with Shenque (CV 8) Bazhenxue moxibustion is an effective method for senile habitual constipation.

OBJECTIVETo explore an effective method for treatment of tennis elbow.

METHODSOne hundred and twenty-eight cases were randomly divided into a treatment group and a control group, 64 cases in each group. The treatment group were treated by electroacupuncture at Hegu (LI 4) and Taichong (LR 3), combined with cake-seperated moxibustion at Ashi points and Shousanli (LI 10) in the affected elbow, and the control group by routine block treatment of 1 mL lidocane and 25 mg prednisone.

RESULTSThe cured rate and the effective rate were 40.6% and 93.7% in the treatment group, and 25.0% and 78.1% in the control group, respectively, with a significant difference between the two groups (P < 0.05).

CONCLUSIONElectroacupuncture combined with cake-separated moxibustion is an effective method for tennis elbow.

This study was designed to determine the impact of chrysoeriol on proliferation and cell cycle progression in the human multiple myeloma cell lines RPMI 8226 and KM3, and its related molecular mechanisms. Chryseoriol was identified by using the phosphorylated AKT-specific cytoblot high throughput assay. CCK-8 assay was employed to examine the growth inhibition rate and IC(50) (48 h) in peripheral blood mononuclear cells (PBMNCs), RPMI 8226 and KM3 cells treated with chrysoeriol at various concentrations. Cells were labeled with 5-6-carboxyfluorescein diacetate succinimidyl ester (CFSE), and the proliferation dynamics was detected by flow cytometry and analyzed with ModFit software. The cell cycles of RPMI 8226 and KM3 cells were measured by flow cytometry when the IC(50) concentration of chrysoeriol was adopted. The alterations in cell-cycle related proteins (Cyclin B1, Cyclin D1, p21) and proteins in PI3K-AKT-mTOR pathway were determined by Western blot analysis. The results showed the proliferation of multiple myeloma cells was significantly inhibited by chrysoeriol, resulting in cell cycle arrest in G(2)/M phase. Chrysoeriol could significantly reduce the expression of p-AKT (s473) and p-4eBP1 (t37/46) protein, meanwhile enhanced Cyclin B1 and p21 protein expression. Similar effects were not observed in PBMNCs from normal donors. It was concluded that chrysoeriol was a selective PI3K-AKT-mTOR pathway inhibitor. It restrained the proliferation of human multiple myeloma cells, but didn't affect proliferation of PBMNCs from normal donors. It might exhibit the cell cycle regulatory effect via the inhibition of PI3K-AKT-mTOR signal pathway.
Antineoplastic Agents, Phytogenic ; pharmacology ; Aspalathus ; chemistry ; Cell Cycle ; drug effects ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Flavones ; isolation & purification ; pharmacology ; Humans ; Multiple Myeloma ; pathology ; Phosphatidylinositol 3-Kinases ; antagonists & inhibitors ; metabolism ; Proto-Oncogene Proteins c-akt ; antagonists & inhibitors ; metabolism ; Signal Transduction ; drug effects ; physiology ; TOR Serine-Threonine Kinases ; antagonists & inhibitors ; metabolism

Pan-immune inflammation value(PIV)is a comprehensive immune inflammatory bio-marker based on complete blood cell counts,which has been proven to predict treatment response and survival outcomes for different types of tumors.However,the predictive value of the PIV varies in dif-ferent strategies for tumor treatment.This paper aims to systematically review the latest progress of PIV in predicting survival outcomes and tumor prognosis for immunotherapy,radiotherapy,targeted therapy,endocrine therapy,surgical treatment and neoadjuvant therapy,and analyze its existing challenges and issues,as well as look forward to its future development direction and application prospects.

Objective To investigate the correlation between pre-treatment pan-immune inflam-mation value(PIV)and clinicopathological features in esophageal squamous cell carcinoma(ESCC)patients with postoperative adjuvant radiotherapy and evaluate its value in prognosis assessment com-bined with T stage.Methods A retrospective analysis was conducted on data of 85 ESCC patients with postoperative adjuvant radiotherapy in the Department of Radiation Oncology of the Affiliated Hospital of Yangzhou University from January 2019 to January 2023.The receiver operating character-istic(ROC)curve was drew to obtain the optimal cut-off value of PIV and other immune-inflammatory biomarkers.The area under the curve(AUC)and clinical applicability of PIV and other immune-in-flammatory biomarkers were compared based on the ROC curve and decision curve analysis(DC A).According to the optimal cut-off value,patients were divided into high PIV group and low PIV group,and the correlation between PIV level and clinicopathological features of ESCC was evaluated.Kaplan-Meier method was used for survival analysis,the Cox proportional hazards model was used for multivariate analysis,and a risk stratification model combining PIV and T stage was established by recursive partitioning analysis(RPA).Results The optimal cut-off value of pre-treatment PIV was determined as 187.22 based on the ROC curve.The AUC of PIV was 0.679,which was greater than 0.640,0.583,0.656 and 0.644 of the other four immune-inflammatory biomarkers such as the systemic immune-inflammation index(SII),platelet-to-lymphocyte ratio(PLR),monocyte-to-lymphocyte ratio(MLR),and neutrophil-to-lymphocyte ratio(NLR).The 85 patients were divided into low PIV group(＜187.22,n=48)and high PIV group(≥187.22,n=37).The level of PIV was significantly correlated with tumor diameter(P＜0.05).The 3-year overall survival(OS)(75.6％versus 30.6％,P＜0.001)and 3-year disease-free survival(DFS)(56.1％versus 21.0％,P＜0.001)were significantly higher in the low PIV group than the high PIV group.Tumor diameter,T stage and PIV were independent factors affecting OS in ESCC patients(P＜0.05),and T stage and PIV were independent factors affecting DFS in ESCC patients(P＜0.05).A new stag-ing system with three risk groups was established by the RPA stratification model based on T stage and PIV,which further improved the predictive value of prognosis compared with T stage or PIV alone.Conclusion Pre-treatment PIV is helpful in predicting the prognosis of ESCC patients with postoper-ative adjuvant radiotherapy,and the combination of PIV and T stage can improve the predictive value.

Pan-immune inflammation value(PIV)is a comprehensive immune inflammatory bio-marker based on complete blood cell counts,which has been proven to predict treatment response and survival outcomes for different types of tumors.However,the predictive value of the PIV varies in dif-ferent strategies for tumor treatment.This paper aims to systematically review the latest progress of PIV in predicting survival outcomes and tumor prognosis for immunotherapy,radiotherapy,targeted therapy,endocrine therapy,surgical treatment and neoadjuvant therapy,and analyze its existing challenges and issues,as well as look forward to its future development direction and application prospects.